Rhodococcus fascians DSM 20669 is an aerobe, mesophilic, Gram-positive plant pathogen that was isolated from Fasciation of sweet peas .
Gram-positive oval-shaped aerobe mesophilic plant pathogen genome sequence 16S sequence| @ref 20215 |
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| Domain Bacillati |
| Phylum Actinomycetota |
| Class Actinomycetes |
| Order Mycobacteriales |
| Family Nocardiaceae |
| Genus Rhodococcus |
| Species Rhodococcus fascians |
| Full scientific name Rhodococcus fascians (Tilford 1936) Goodfellow 1984 |
| Synonyms (4) |
| @ref | Name | Growth | Medium link | Composition | |
|---|---|---|---|---|---|
| 8991 | TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) | Medium recipe at MediaDive | Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water | ||
| 8991 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | Medium recipe at MediaDive | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water | ||
| 37045 | MEDIUM 3 - Columbia agar | Columbia agar (39.000 g);distilled water (1000.000 ml) | |||
| 119291 | CIP Medium 3 | Medium recipe at CIP |
| 67770 | Observationquinones: MK-8(H2) |
| @ref | Chebi-ID | Metabolite | Utilization activity | Kind of utilization tested | |
|---|---|---|---|---|---|
| 119291 | 16947 ChEBI | citrate | + | carbon source | |
| 68379 | 17634 ChEBI | D-glucose | - | fermentation | from API Coryne |
| 68379 | 16899 ChEBI | D-mannitol | - | fermentation | from API Coryne |
| 68379 | 16988 ChEBI | D-ribose | - | fermentation | from API Coryne |
| 68379 | 65327 ChEBI | D-xylose | - | fermentation | from API Coryne |
| 68379 | 4853 ChEBI | esculin | - | hydrolysis | from API Coryne |
| 119291 | 4853 ChEBI | esculin | - | hydrolysis | |
| 68379 | 5291 ChEBI | gelatin | - | hydrolysis | from API Coryne |
| 68379 | 28087 ChEBI | glycogen | - | fermentation | from API Coryne |
| 68379 | 17716 ChEBI | lactose | - | fermentation | from API Coryne |
| 68379 | 17306 ChEBI | maltose | - | fermentation | from API Coryne |
| 68379 | 17632 ChEBI | nitrate | - | reduction | from API Coryne |
| 119291 | 17632 ChEBI | nitrate | - | reduction | |
| 119291 | 17632 ChEBI | nitrate | - | respiration | |
| 119291 | 16301 ChEBI | nitrite | - | reduction | |
| 68379 | 17992 ChEBI | sucrose | - | fermentation | from API Coryne |
| 68379 | 16199 ChEBI | urea | - | hydrolysis | from API Coryne |
| @ref | Value | Activity | Ec | |
|---|---|---|---|---|
| 119291 | alcohol dehydrogenase | - | 1.1.1.1 | |
| 68379 | alkaline phosphatase | + | 3.1.3.1 | from API Coryne |
| 68382 | alpha-chymotrypsin | - | 3.4.21.1 | from API zym |
| 68382 | alpha-fucosidase | - | 3.2.1.51 | from API zym |
| 68382 | alpha-galactosidase | - | 3.2.1.22 | from API zym |
| 68379 | alpha-glucosidase | + | 3.2.1.20 | from API Coryne |
| 68382 | alpha-mannosidase | - | 3.2.1.24 | from API zym |
| 119291 | amylase | - | ||
| 68382 | beta-galactosidase | - | 3.2.1.23 | from API zym |
| 119291 | beta-galactosidase | + | 3.2.1.23 | |
| 68379 | beta-galactosidase | - | 3.2.1.23 | from API Coryne |
| 68382 | beta-glucosidase | - | 3.2.1.21 | from API zym |
| 68379 | beta-glucosidase | - | 3.2.1.21 | from API Coryne |
| 68382 | beta-glucuronidase | - | 3.2.1.31 | from API zym |
| 68379 | beta-glucuronidase | - | 3.2.1.31 | from API Coryne |
| 119291 | caseinase | - | 3.4.21.50 | |
| 119291 | catalase | + | 1.11.1.6 | |
| 119291 | DNase | - | ||
| 68382 | esterase lipase (C 8) | + | from API zym | |
| 119291 | gamma-glutamyltransferase | - | 2.3.2.2 | |
| 119291 | gelatinase | - | ||
| 68379 | gelatinase | - | from API Coryne | |
| 119291 | lecithinase | - | ||
| 68382 | lipase (C 14) | - | from API zym | |
| 119291 | lysine decarboxylase | - | 4.1.1.18 | |
| 68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 | from API zym |
| 68379 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 | from API Coryne |
| 68382 | naphthol-AS-BI-phosphohydrolase | - | from API zym | |
| 119291 | ornithine decarboxylase | - | 4.1.1.17 | |
| 119291 | oxidase | - | ||
| 68379 | pyrazinamidase | + | 3.5.1.B15 | from API Coryne |
| 119291 | tryptophan deaminase | - | ||
| 119291 | tween esterase | - | ||
| 119291 | urease | + | 3.5.1.5 | |
| 68379 | urease | - | 3.5.1.5 | from API Coryne |
| 68382 | valine arylamidase | - | from API zym |
| @ref | Control | Alkaline phosphatase | Esterase (C 4) | 2-naphtyl caprylateEsterase Lipase (C 8) | Lipase (C 14) | L-leucyl-2-naphthylamideLeucine arylamidase | L-valyl-2-naphthylamideValine arylamidase | L-cystyl-2-naphthylamideCystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 8991 | - | - | - | + | - | +/- | - | +/- | - | - | +/- | - | - | - | - | +/- | - | - | - | - | |
| 119291 | - | + | + | + | - | + | - | + | + | - | + | - | - | - | - | + | - | - | - | - |
| @ref | Sample type | Host species | |
|---|---|---|---|
| 67770 | Fasciation of sweet peas (Lathyrus odoratus) | Lathyrus odoratus |
Global distribution of 16S sequence X81930 (>99% sequence identity) for Rhodococcus fascians from Microbeatlas ![]()
| @ref | Description | Assembly level | INSDC accession | BV-BRC accession | NCBI tax ID | Score | |
|---|---|---|---|---|---|---|---|
| 67770 | ASM189478v1 assembly for Rhodococcoides fascians NBRC 12155 = LMG 3623 | contig | 1219015 | 71.62 | |||
| 67770 | R_fas_LMG3623 assembly for Rhodococcoides fascians NBRC 12155 = LMG 3623 | scaffold | 1219015 | 64.25 | |||
| 66792 | ASM33395v1 assembly for Rhodococcus sp. AW25M09 | contig | 1268303 | 45.77 |
| @ref | Description | Accession | Length | Database | NCBI tax ID | |
|---|---|---|---|---|---|---|
| 20218 | R.fascians 16S rRNA gene (ATCC 12974T) | X81930 | 1314 | 1828 | ||
| 20218 | R.fascians (DSM 20669) 16S rRNA gene | X79186 | 1424 | 1828 | ||
| 124043 | Rhodococcus fascians strain LMG 3623(T) 16S ribosomal RNA gene, partial sequence. | MN686685 | 510 | 1828 | ||
| 124043 | Rhodococcus fascians strain LMG 3623(T) 16S ribosomal RNA gene, partial sequence. | MN686663 | 593 | 1828 |
| @ref | GC-content (mol%) | Method | |
|---|---|---|---|
| 67770 | 64.4 | genome sequence analysis |
| @ref | Trait | Model | Prediction | Confidence in % | In training data |
|---|---|---|---|---|---|
| 125439 | oxygen_tolerance | BacteriaNetⓘ | obligate aerobe | 98.50 | no |
| 125439 | gram_stain | BacteriaNetⓘ | positive | 96.00 | no |
| 125439 | motility | BacteriaNetⓘ | no | 80.70 | no |
| 125439 | spore_formation | BacteriaNetⓘ | no | 50.40 | no |
| @ref | Trait | Model | Prediction | Confidence in % | In training data |
|---|---|---|---|---|---|
| 125438 | gram-positive | gram-positiveⓘ | yes | 89.10 | no |
| 125438 | anaerobic | anaerobicⓘ | no | 97.42 | yes |
| 125438 | spore-forming | spore-formingⓘ | no | 59.96 | no |
| 125438 | aerobic | aerobicⓘ | yes | 88.31 | yes |
| 125438 | thermophilic | thermophileⓘ | no | 96.00 | no |
| 125438 | flagellated | motile2+ⓘ | no | 91.50 | no |
| Title | Authors | Journal | DOI | Year | |
|---|---|---|---|---|---|
| Draft Genome Sequence of the Actinomycete Rhodococcus sp. Strain AW25M09, Isolated from the Hadsel Fjord, Northern Norway. | Hjerde E, Pierechod MM, Williamson AK, Bjerga GE, Willassen NP, Smalas AO, Altermark B. | Genome Announc | 10.1128/genomea.00055-13 | 2013 | |
| Horizontal Spread of Rhodococcus equi Macrolide Resistance Plasmid pRErm46 across Environmental Actinobacteria. | Alvarez-Narvaez S, Giguere S, Berghaus LJ, Dailey C, Vazquez-Boland JA. | Appl Environ Microbiol | 10.1128/aem.00108-20 | 2020 | |
| Improved-high-quality draft genome sequence of Rhodococcus sp. JG-3, a eurypsychrophilic Actinobacteria from Antarctic Dry Valley permafrost. | Goordial J, Raymond-Bouchard I, Ronholm J, Shapiro N, Woyke T, Whyte L, Bakermans C. | Stand Genomic Sci | 10.1186/s40793-015-0043-8 | 2015 | |
| Assessing the effect of temperature on Rhodococcus metabolite production. | Macias-Contreras MI, Millan-Aguinaga N, Parra J, Duncan KR. | Microbiology (Reading) | 10.1099/mic.0.001598 | 2025 | |
| Identification of a Novel Class of Photolyases as Possible Ancestors of Their Family. | Xu L, Chen S, Wen B, Shi H, Chi C, Liu C, Wang K, Tao X, Wang M, Lv J, Yan L, Ling L, Zhu G. | Mol Biol Evol | 10.1093/molbev/msab191 | 2021 | |
| Validation of the Peel Plate Staphylococcus Aureus (SA) Test for Enumeration of S. aureus in Selected Foods and Non-Cultured Dairy Products: AOAC Performance Tested MethodSM 082401. | Salter RS, Durbin GW, Li S, Gilbert M, Crowley ES, Deterding A, Bastin B. | J AOAC Int | 10.1093/jaoacint/qsae083 | 2025 | |
| Fasciation induction by the phytopathogen Rhodococcus fascians depends upon a linear plasmid encoding a cytokinin synthase gene. | Crespi M, Messens E, Caplan AB, van Montagu M, Desomer J. | EMBO J | 10.1002/j.1460-2075.1992.tb05116.x | 1992 | |
| Genome Sequences of Plant-Associated Rhodococcus sp. Isolates from Tunisia. | Dhaouadi S, Win J, Mougou AH, Harant A, Kamoun S, Rhouma A. | Microbiol Resour Announc | 10.1128/mra.00293-20 | 2020 | |
| Surfactants tailored by the class Actinobacteria. | Kugler JH, Le Roes-Hill M, Syldatk C, Hausmann R. | Front Microbiol | 10.3389/fmicb.2015.00212 | 2015 | |
| Genetic analysis of Clavibacter toxicus, the agent of annual ryegrass toxicity. | Johnston MS, Sutherland SS, Constantine CC, Hampson DJ. | Epidemiol Infect | 10.1017/s0950268800001588 | 1996 | |
| Microorganisms and enzymes involved in polybutylene adipate terephthalate biodegradation. | Fernandes M, Vicente AA, Salvador AF. | Appl Microbiol Biotechnol | 10.1007/s00253-025-13565-4 | 2025 | |
| The ant Lasius niger is a new source of bacterial enzymes with biotechnological potential for bleaching dye. | Diez-Mendez A, Garcia-Fraile P, Solano F, Rivas R. | Sci Rep | 10.1038/s41598-019-51669-w | 2019 | |
| Characterization of antarctic hydrocarbon-degrading bacteria capable of producing bioemulsifiers. | Yakimov MM, Giuliano L, Bruni V, Scarfi S, Golyshin PN | New Microbiol | 1999 | ||
| Pulsed-field gel electrophoresis analysis of the genome of Rhodococcus fascians: genome size and linear and circular replicon composition in virulent and avirulent strains. | Pisabarro A, Correia A, Martin JF | Curr Microbiol | 10.1007/s002849900314 | 1998 | |
| Characterization of the rrnB operon of the plant pathogen Rhodococcus fascians and targeted integrations of exogenous genes at rrn loci. | Pisabarro A, Correia A, Martin JF | Appl Environ Microbiol | 10.1128/AEM.64.4.1276-1282.1998 | 1998 | |
| Conjugative transfer of cadmium resistance plasmids in Rhodococcus fascians strains. | Desomer J, Dhaese P, Van Montagu M | J Bacteriol | 10.1128/jb.170.5.2401-2405.1988 | 1988 | |
| The Predatory Myxobacterium Citreicoccus inhibens gen. nov. sp. nov. Showed Antifungal Activity and Bacteriolytic Property against Phytopathogens. | Zhou Y, Yi S, Zang Y, Yao Q, Zhu H. | Microorganisms | 10.3390/microorganisms9102137 | 2021 | |
| Rhodococcus sovatensis sp. nov., an actinomycete isolated from the hypersaline and heliothermal Lake Ursu. | Tancsics A, Mathe I, Benedek T, Toth EM, Atasayar E, Sproer C, Marialigeti K, Felfoldi T, Kriszt B | Int J Syst Evol Microbiol | 10.1099/ijsem.0.001514 | 2017 | |
| Rhodococcus cercidiphylli sp. nov., a new endophytic actinobacterium isolated from a Cercidiphyllum japonicum leaf. | Li J, Zhao GZ, Chen HH, Qin S, Xu LH, Jiang CL, Li WJ | Syst Appl Microbiol | 10.1016/j.syapm.2008.03.004 | 2008 | |
| Rhodococcus kyotonensis sp. nov., a novel actinomycete isolated from soil. | Li B, Furihata K, Ding LX, Yokota A | Int J Syst Evol Microbiol | 10.1099/ijs.0.64770-0 | 2007 | |
| Rhodococcus yunnanensis sp. nov., a mesophilic actinobacterium isolated from forest soil. | Zhang YQ, Li WJ, Kroppenstedt RM, Kim CJ, Chen GZ, Park DJ, Xu LH, Jiang CL | Int J Syst Evol Microbiol | 10.1099/ijs.0.63390-0 | 2005 |
| #8991 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20669 |
| #20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 ) |
| #20216 | Curators of the HKI: Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI) . Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI): |
| #20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 ) |
| #37045 | ; Curators of the CIP; |
| #59099 | Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 51073 |
| #66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) . |
| #67770 | Japan Collection of Microorganism (JCM) ; Curators of the JCM; |
| #68379 | Automatically annotated from API Coryne . |
| #68382 | Automatically annotated from API zym . |
| #69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta . |
| #119291 | Collection of Institut Pasteur ; Curators of the CIP; CIP 104713 |
| #124043 | Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy . |
| #125438 | Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 ) |
| #125439 | Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 . |
| #126262 | A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 ) |
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