Rhodococcus hoagii (DSM 20295, ATCC 7005, CCUG 20991)

Name: Rhodococcus hoagii (DSM 20295, ATCC 7005, CCUG 20991)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 10842

Type strain

Culture col. no. DSM 20295 ATCC 7005 CCUG 20991 CIP 81.17 JCM 1319 7 more

NCBI tax ID(s) 43767

Special Collections DSMZ CCUG JCM CIP Wink compendium JMRC

History DSMZ <- H.G. Schlegel <- K. Komagata <- ATCC <- F.S. Orcutt JCM <-- K. Suzuki CNF 007 <-- AJ 1408 <-- ATCC 7005 <-- F. S. Orcutt. CIP <- 1981, NCTC <- ATCC <- F.S. Orcutt

Links

Rhodococcus hoagii DSM 20295 is a human pathogen of the family Nocardiaceae.

human pathogen genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 34830

ATCC 7005,NCTC 10673,CCUG 20991,CCUG 37875,DSM 20295,JCM 1319,CCRC 12158,IAM 12428,CIP 81.17,IMET 11373,AS 1.1887,BCRC 12158,CGMCC 1.1887,NBRC 103062

@ref 20215

Last LPSN update 2026-05-29 08:27:25

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Mycobacteriales

Family Nocardiaceae

Genus Rhodococcus

Species Rhodococcus hoagii

Full scientific name Rhodococcus hoagii (Morse 1912) Kämpfer et al. 2014

Synonyms (2)

Corynebacterium hoagii
"Bacillus hoagii"

BacDive ID	Other strains from Rhodococcus hoagii (31)	Type strain
10849	R. hoagii RW2/1, DSM 30791
130476	R. hoagii DSM 26605, NCIMB 10027
130477	R. hoagii VI1, DSM 26606
130478	R. hoagii CV1, DSM 26607
130479	R. hoagii GV1, DSM 26608
130480	R. hoagii GV2, DSM 26609
143270	R. hoagii CCUG 18145
143383	R. hoagii CCUG 18812
145182	R. hoagii CCUG 27822, ATCC 33703
145183	R. hoagii CCUG 27823, ATCC 33707
145443	R. hoagii CCUG 28565
145469	R. hoagii CCUG 28631
146163	R. hoagii CCUG 30487
146579	R. hoagii CCUG 31490
147113	R. hoagii CCUG 32889
147172	R. hoagii CCUG 32992
149690	R. hoagii CCUG 38197
152324	R. hoagii CCUG 46442
153177	R. hoagii CCUG 48649
153564	R. hoagii CCUG 50191 A
154560	R. hoagii CCUG 54412
161313	R. hoagii JCM 1312, ATCC 10146, IAM 12444, IMSNU 20113, ...
161314	R. hoagii JCM 1313, IAM 12445
161315	R. hoagii JCM 1314, IAM 12446
165418	R. hoagii JCM 6817
165419	R. hoagii JCM 6818
165420	R. hoagii JCM 6819, BCRC 13634
165421	R. hoagii JCM 6820
165429	R. hoagii JCM 6828
165430	R. hoagii JCM 6829
165433	R. hoagii JCM 6832

Morphology

Cell morphology

@ref	Motility	Confidence	Gram stain
125438		92.5
125438		93.242	positive

Colony morphology

@ref	Colony color	Incubation period	Medium used
19790	Bright red orange (2008)	10-14 days	ISP 2
19790	Beige (1001)	10-14 days	ISP 3
19790	Pastel yellow (1034)	10-14 days	ISP 4
19790	Pastel yellow (1034)	10-14 days	ISP 6

Multicellular morphology

@ref	Forms multicellular complex	Medium name
19790		ISP 2
19790		ISP 3
19790		ISP 4
19790		ISP 6

Multimedia

@ref:	8698
multimedia content:	DSM_20295.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_20295.jpg
caption:	Medium 693 28°C
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
8698	COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)	Medium recipe at MediaDive	Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
8698	TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)	Medium recipe at MediaDive	Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water
19790	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19790	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19790	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19790	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
37343	MEDIUM 3 - Columbia agar		Columbia agar (39.000 g);distilled water (1000.000 ml)
119111	CIP Medium 72	Medium recipe at CIP
119111	CIP Medium 3	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
8698	positive	growth	28
19790	positive	optimum	28
37343	positive	growth	30
67770	positive	growth	28

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125438	aerobe	92.071
125439	obligate aerobe	98.174

Spore formation

@ref	Spore formation	Confidence
125439		95.142

Compound production

8698

Compounddienes

Halophily

@ref	Salt	Growth	Tested relation	Concentration
19790	NaCl	positive	maximum	5 %

Murein

@ref	Murein short key	Type
8698	A31	A1gamma m-Dpm-direct

Observation

67770

Observationquinones: MK-8(H₂)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68368	27613 ChEBI	amygdalin	-	fermentation	from API 20E
19790	22599 ChEBI	arabinose	-
68368	29016 ChEBI	arginine	-	hydrolysis	from API 20E
19790	62968 ChEBI	cellulose	-
68368	16947 ChEBI	citrate	-	assimilation	from API 20E
68379	17634 ChEBI	D-glucose	-	fermentation	from API Coryne
68368	17634 ChEBI	D-glucose	-	fermentation	from API 20E
68379	16899 ChEBI	D-mannitol	-	fermentation	from API Coryne
68368	16899 ChEBI	D-mannitol	-	fermentation	from API 20E
68379	16988 ChEBI	D-ribose	-	fermentation	from API Coryne
68379	65327 ChEBI	D-xylose	-	fermentation	from API Coryne
68379	4853 ChEBI	esculin	-	hydrolysis	from API Coryne
19790	28757 ChEBI	fructose	-
68379	5291 ChEBI	gelatin	-	hydrolysis	from API Coryne
68368	5291 ChEBI	gelatin	-	hydrolysis	from API 20E
19790	17234 ChEBI	glucose	+
68379	28087 ChEBI	glycogen	-	fermentation	from API Coryne
68368	30849 ChEBI	L-arabinose	-	fermentation	from API 20E
68368	62345 ChEBI	L-rhamnose	-	fermentation	from API 20E
68379	17716 ChEBI	lactose	-	fermentation	from API Coryne
68368	25094 ChEBI	lysine	-	degradation	from API 20E
68379	17306 ChEBI	maltose	-	fermentation	from API Coryne
19790	29864 ChEBI	mannitol	-
68368	28053 ChEBI	melibiose	-	fermentation	from API 20E
19790	17268 ChEBI	myo-inositol	-
68368	17268 ChEBI	myo-inositol	-	fermentation	from API 20E
68368	18257 ChEBI	ornithine	-	degradation	from API 20E
19790	16634 ChEBI	raffinose	-
19790	26546 ChEBI	rhamnose	-
68368	30911 ChEBI	sorbitol	-	fermentation	from API 20E
19790	17992 ChEBI	sucrose	-
68368	17992 ChEBI	sucrose	-	fermentation	from API 20E
68368	27897 ChEBI	tryptophan	-	energy source	from API 20E
68379	16199 ChEBI	urea	-	hydrolysis	from API Coryne
68368	16199 ChEBI	urea	-	hydrolysis	from API 20E
19790	18222 ChEBI	xylose	-

Metabolite production

@ref	Chebi-ID	Metabolite
68368	15688 ChEBI	acetoin	from API 20E
68368	16136 ChEBI	hydrogen sulfide	from API 20E
68368	35581 ChEBI	indole	from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Indole test
68368	15688 ChEBI	acetoin	+		from API 20E
68368	35581 ChEBI	indole		-	from API 20E

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68379	alkaline phosphatase	+	3.1.3.1	from API Coryne
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68379	alpha-glucosidase	+	3.2.1.20	from API Coryne
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68368	arginine dihydrolase	-	3.5.3.6	from API 20E
68382	beta-galactosidase	-	3.2.1.23	from API zym
68379	beta-galactosidase	-	3.2.1.23	from API Coryne
68368	beta-galactosidase	-	3.2.1.23	from API 20E
68382	beta-glucosidase	-	3.2.1.21	from API zym
68379	beta-glucosidase	-	3.2.1.21	from API Coryne
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68379	beta-glucuronidase	-	3.2.1.31	from API Coryne
68379	catalase	+	1.11.1.6	from API Coryne
68382	cystine arylamidase	+	3.4.11.3	from API zym
68382	esterase (C 4)	-		from API zym
68382	esterase lipase (C 8)	+		from API zym
68379	gelatinase	-		from API Coryne
68368	gelatinase	-		from API 20E
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68368	lysine decarboxylase	-	4.1.1.18	from API 20E
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68379	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API Coryne
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
68368	ornithine decarboxylase	-	4.1.1.17	from API 20E
68379	pyrazinamidase	-	3.5.1.B15	from API Coryne
68379	pyrrolidonyl arylamidase	-	3.4.19.3	from API Coryne
68382	trypsin	+	3.4.21.4	from API zym
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
68379	urease	-	3.5.1.5	from API Coryne
68368	urease	-	3.5.1.5	from API 20E
68382	valine arylamidase	+		from API zym

API 20E

@ref	ONPG	ADH (Arg)	LDC (Lys)	ODC	CIT	H2S productionH2S	URE	TDA (Trp)	IND	Acetoin production (Voges Proskauer test)VP	GEL	GLU	MAN	INO	Sor	RHA	SAC	MEL	AMY	ARA	OX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	OF-O	OF-F
19790	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API coryne

@ref	Reduction of nitrateNIT	PYZ	PYRA	PAL	beta GUR	beta GAL	alpha GLU	beta NAG	ESC	URE	GEL	Control fermentationControl	GLU	RIB	XYL	MAN	MAL	LAC	SAC	GLYG	CAT
19790	-	-	-	+	-	-	+	-	-	-	-	not determinedn.d.	-	-	-	-	-	-	+	-	not determinedn.d.
65634	+	-	-	+	-	-	+	-	-	-	-	-	not determinedn.d.	-	-	-	-	-	-	-	+
8698	+	-	-	+	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	+

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
19790	not determinedn.d.	+	-	+	-	+	+	+	+	-	+	+	-	-	-	+	-	-	-	-
65634	-	+	-	+	-	+	+	+	+	-	+	+	-	-	-	+	-	-	-	-

Isolation, sampling and environmental information

Taxonmaps

69479

Global distribution of 16S sequence X82052 (>99% sequence identity) for Rhodococcus from Microbeatlas

Aquatic Samples	4265
Soil Samples	18366
Animal Samples	5588
Plant Samples	3054
Total Samples	31273

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
8698	2	Risk group (German classification) According to TRBA 466
19790	2	Risk group (German classification) According to TRBA 466
119111	2	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM164664v1 assembly for Prescottella equi DSM 20295	contig	GCA_001646645	43767.10	2744054612	43767	20.58

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
8698	C.hoagii 16S ribosomal DNA	X82052	1306		43767
124043	Rhodococcus hoagii strain DSM 20295 16S ribosomal RNA gene, partial sequence.	MZ276292	1419		43767

GC content

@ref	GC-content (mol%)	Method
8698	64.6
67770	64.6	thermal denaturation, midpoint method (Tm)
67770	68.8	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM164664v1 assembly for Prescottella equi DSM 20295
NCBI: GCA_001646645

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	gram_stain	BacteriaNetⓘ	positive	76.07	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	98.17	no
125439	motility	BacteriaNetⓘ	no	64.56	no
125439	spore_formation	BacteriaNetⓘ	no	95.14	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Rhodococcus equi strain DSM 20295
PATRIC: 43767.10

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	93.24	no
125438	anaerobic	anaerobicⓘ	no	97.87	no
125438	spore-forming	spore-formingⓘ	no	56.57	no
125438	aerobic	aerobicⓘ	yes	92.07	no
125438	thermophilic	thermophileⓘ	no	97.50	yes
125438	flagellated	motile2+ⓘ	no	92.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Genomic analysis of a novel Rhodococcus (Prescottella) equi isolate from a bovine host.	Paterson ML, Ranasinghe D, Blom J, Dover LG, Sutcliffe IC, Lopes B, Sangal V.	Arch Microbiol	10.1007/s00203-019-01695-z	2019
	Comparative Genomic Analysis of Rhodococcus equi: An Insight into Genomic Diversity and Genome Evolution.	Ying J, Ye J, Xu T, Wang Q, Bao Q, Li A.	Int J Genomics	10.1155/2019/8987436	2019
Genetics	Genome-Based Taxonomic Classification of the Phylum Actinobacteria.	Nouioui I, Carro L, Garcia-Lopez M, Meier-Kolthoff JP, Woyke T, Kyrpides NC, Pukall R, Klenk HP, Goodfellow M, Goker M.	Front Microbiol	10.3389/fmicb.2018.02007	2018
	A Boron-Dependent Antibiotic Derived from a Calcium-Dependent Antibiotic.	Chiou SL, Chen YJ, Lee CT, Ho MN, Miao J, Kuo PC, Hsu CC, Lin YS, Chu J.	Angew Chem Int Ed Engl	10.1002/anie.202317522	2024
Phylogeny	Conservation of Rhodococcus equi (Magnusson 1923) Goodfellow and Alderson 1977 and rejection of Rhodococcus hoagii (Morse 1912) Kampfer et al. 2014.	Vazquez-Boland JA, Scortti M, Meijer WG	Int J Syst Evol Microbiol	10.1099/ijsem.0.004090	2020
Phylogeny	Rhodococcus agglutinans sp. nov., an actinobacterium isolated from a soil sample.	Guo QQ, Ming H, Meng XL, Duan YY, Gao R, Zhang JX, Huang JR, Li WJ, Nie GX	Antonie Van Leeuwenhoek	10.1007/s10482-015-0421-7	2015
Phylogeny	Rhodococcus soli sp. nov., an actinobacterium isolated from soil using a resuscitative technique.	Li SH, Yu XY, Park DJ, Hozzein WN, Kim CJ, Shu WS, Wadaan MA, Ding LX, Li WJ	Antonie Van Leeuwenhoek	10.1007/s10482-014-0334-x	2014
Phylogeny	Rhodococcus defluvii sp. nov., isolated from wastewater of a bioreactor and formal proposal to reclassify [Corynebacterium hoagii] and Rhodococcus equi as Rhodococcus hoagii comb. nov.	Kampfer P, Dott W, Martin K, Glaeser SP	Int J Syst Evol Microbiol	10.1099/ijs.0.053322-0	2013

References

#8698	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20295
#19790	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20216	Curators of the JMRC: Jena Microbial Resource Collection (JMRC):
#37343	; Curators of the CIP;
#65634	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 20991
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68368	Automatically annotated from API 20E .
#68379	Automatically annotated from API Coryne .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#119111	Collection of Institut Pasteur ; Curators of the CIP; CIP 81.17
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Rhodococcus hoagii (DSM 20295, ATCC 7005, CCUG 20991)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Compound production

Halophily

Murein

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API 20E

API coryne

API zym

Isolation, sampling and environmental information

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_001646645

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

Help

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