Name: Mycobacterium chelonae RAMC
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 8570

Type strain:

Species: Mycobacterium chelonae

Strain Designation: RAMC

Culture col. no.: DSM 43804, ATCC 35752, CCUG 47445, IMET 10609, JCM 6388, NCTC 946, TMC 1544, CIP 104535, KCTC 9505, NCIMB 1474

Strain history: M. Tsukamura 19054 <-- J. L. Stanford 78 <-- NCTC 946 <-- F. M. Perry strain Friedman.

NCBI tax ID(s): 1460372 (strain), 1774 (species)

Other strains from Mycobacterium chelonae

M. chelonae MOTT36W, KCTC 29127, JCM 32454 (type strain)
M. chelonae CCUG 27981, DSM 44159, ATCC 35750, ...
M. chelonae 10546, DSM 43217, ATCC 19236, SN ...
M. chelonae Magnussen 921, DSM 43283, ATCC ...
M. chelonae DSM 43483, ATCC 19535, SN 282
M. chelonae DSM 43484, ATCC 19536, SN 283
M. chelonae DSM 43485, ATCC 19537, SN 284
M. chelonae DSM 43486, ATCC 19538, SN 285
M. chelonae DSM 43488, ATCC 19539, SN 287
M. chelonae DSM 43489, ATCC 19540, IMET 10657, ...
M. chelonae DSM 43490, SN 289
M. chelonae 788, DSM 46627, IMET 10611
M. chelonae STI07275(IMET), N95,
M. chelonae STI07367(IMET),
M. chelonae CCUG 27980, TMC 1524
M. chelonae CCUG 28057
M. chelonae CCUG 34239
M. chelonae CCUG 35585
M. chelonae CCUG 37827

Section

Mycobacterium chelonae RAMC is an aerobe, mesophilic human pathogen that was isolated from tortoise tubercle.

aerobe
human pathogen
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Mycobacteriales*
	Family	*Mycobacteriaceae*
	Genus	*Mycobacterium*
	Species	**Mycobacterium chelonae**
	Full Scientific Name (PNU)	Mycobacterium chelonae corrig. Bergey et al. 1923 (Approved Lists 1980)

	Synonym	Mycobacterium chelonei
	Synonym	Mycobacteroides chelonae

Information on morphological and physiological properties Morphology

[Ref.: #57692]

Incubation period

4-5 days

Multimedia content

[Ref.: #11257]

Caption:

Medium 645 37°C

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Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

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Culture medium

MIDDLEBROOK MEDIUM (DSMZ Medium 645)

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Culture medium growth

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Culture medium link

Medium recipe provided by DSMZ

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Culture medium composition

expand / minimize

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Culture medium

MEDIUM 55 - for Mycobacterium

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Culture medium growth

	Kind of temperature		Temperature
[Ref.: #11257]		growth	37 °C
[Ref.: #36330]		growth	37 °C
[Ref.: #43559]		growth	25-30 °C
[Ref.: #43559]	no	growth	45 °C
[Ref.: #43559]	no	growth	37 °C
[Ref.: #57692]		growth	30 °C
[Ref.: #67770]		growth	28 °C

[Ref.: #11257]	Temperature range	mesophilic
[Ref.: #36330]	Temperature range	mesophilic
[Ref.: #43559]	Temperature range	mesophilic
[Ref.: #43559]	Temperature range	thermophilic
[Ref.: #43559]	Temperature range	mesophilic
[Ref.: #57692]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

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Oxygen tolerance

aerobe

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Observation

quinones: MK-9(H₂)

	Enzyme	Enzyme activity	EC number
[Ref.: #43559]	acid phosphatase	+	3.1.3.2
[Ref.: #43559]	alkaline phosphatase	-	3.1.3.1
[Ref.: #43559]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #43559]	alpha-fucosidase	-	3.2.1.51
[Ref.: #43559]	alpha-galactosidase	-	3.2.1.22
[Ref.: #43559]	alpha-glucosidase	+	3.2.1.20
[Ref.: #43559]	alpha-mannosidase	+	3.2.1.24
[Ref.: #43559]	beta-galactosidase	+/-	3.2.1.23
[Ref.: #43559]	beta-glucosidase	+	3.2.1.21
[Ref.: #43559]	beta-glucuronidase	-	3.2.1.31
[Ref.: #43559]	cystine arylamidase	+	3.4.11.3
[Ref.: #43559]	esterase (C 4)	+
[Ref.: #43559]	esterase Lipase (C 8)	+
[Ref.: #43559]	leucine arylamidase	+	3.4.11.1
[Ref.: #43559]	lipase (C 14)	+
[Ref.: #43559]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #43559]	naphthol-AS-BI-phosphohydrolase	-
[Ref.: #43559]	trypsin	-	3.4.21.4
[Ref.: #43559]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #11257]	Sample type/isolated from	tortoise tubercle

[Ref.: #57692]	Sample type/isolated from	Tortoise tubercle

[Ref.: #67770]	Sample type/isolated from	Tortoise tubercle
* marker position based on {}

Isolation sources categories

#Host	#Reptilia	-
#Host Body-Site	-	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AF480594 (>99% sequence identity) for Mycobacteroides from Microbeatlas

Aquatic Samples	6199
Soil Samples	7046
Animal Samples	7007
Plant Samples	1742
Total Samples	21994

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #11257]	Pathogenicity (human)	yes
[Ref.: #11257]	Pathogenicity (animal)	yes
[Ref.: #11257]	Biosafety level	2	Risk group (German classification) According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Mycobacterium chelonae gene for 16S ribosomal RNA, partial sequence, strain: JCM 6388	AB548610	1468	ENA	1774 tax ID	*
[Ref.: #20218]	Mycobacterium chelonae DNA, 16S-23S ribosomal RNA intergenic spacer, partial sequence, strain: JCM 6388	AB548611	294	ENA	1774 tax ID	*
[Ref.: #20218]	Mycobacterium chelonae strain ATCC35752 16S ribosomal RNA (rrs) gene, partial sequence	AF059852	171	ENA	1774 tax ID	*
[Ref.: #20218]	Mycobacterium chelonae 16S ribosomal RNA gene, partial sequence	AF480594	1441	ENA	1774 tax ID	*
[Ref.: #20218]	Mycobacterium chelonae 16S ribosomal RNA gene and 16S-23S intergenic spacer, partial sequence	AY498739	253	ENA	1774 tax ID	*
[Ref.: #20218]	M.chelonae 16S rRNA gene	X82236	1071	ENA	1774 tax ID	*
[Ref.: #20218]	Mycobacterium chelonae murA and 16S rRNA gene	Y13911	904	ENA	1774 tax ID	*
[Ref.: #67770]	Mycobacteroides chelonae strain CIP 104535 16S ribosomal RNA gene, partial sequence	AY457072	1481	ENA	1774 tax ID

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Mycobacteroides chelonae CCUG 47445 CCUG 47445	GCA_001632805	complete	GenBank	1460372 tax ID	*
[Ref.: #66792]	[Mycobacterium] chelonae subsp. chelonae DSM 43804	GCA_004355245	complete	GenBank	2099321 tax ID	*
[Ref.: #66792]	Mycobacteroides chelonae ATCC 35752	GCA_000971805	chromosome	GenBank	1774 tax ID	*
[Ref.: #66792]	Mycobacterium chelonae CCUG 47445	1460372.6	complete	PATRIC	1460372 tax ID	*
[Ref.: #66792]	Mycobacterium chelonae strain ATCC 35752	1774.35	complete	PATRIC	1774 tax ID	*
[Ref.: #66792]	Mycobacterium chelonae strain ATCC 35752	1774.83	wgs	PATRIC	1774 tax ID	*

[Ref.: #67770]	GC-content	63.96 mol%	genome sequence analysis
[Ref.: #67770]	GC-content	63.9 mol%	genome sequence analysis

Availability in culture collections External links

[Ref.: #11257]

Culture collection no.

DSM 43804, ATCC 35752, CCUG 47445, IMET 10609, JCM 6388, NCTC 946, TMC 1544, CIP 104535, KCTC 9505, NCIMB 1474

[Ref.: #20218]

Associated Passport(s) in StrainInfo

152973, 111176, 331917, 152974, 168731, 152975, 111177

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Proposal of Mycobacterium peregrinum sp. nov., nom. rev., and elevation of Mycobacterium chelonae subsp. abscessus (Kubica et al.) to species status: Mycobacterium abscessus comb. nov.	Kusunoki S, Ezaki T	Int J Syst Bacteriol	10.1099/00207713-42-2-240	1992	*
Enzymology	Glutaraldehyde-resistant Mycobacterium chelonae from endoscope washer disinfectors.	Griffiths PA, Babb JR, Bradley CR, Fraise AP	J Appl Microbiol	10.1046/j.1365-2672.1997.00171.x	1997	*
Pathogenicity	Mycobactericidal activity of selected disinfectants using a quantitative suspension test.	Griffiths PA, Babb JR, Fraise AP	J Hosp Infect	10.1016/s0195-6701(99)90048-8	1999	*
Pathogenicity	Reduced glutaraldehyde susceptibility in Mycobacterium chelonae associated with altered cell wall polysaccharides.	Manzoor SE, Lambert PA, Griffiths PA, Gill MJ, Fraise AP	J Antimicrob Chemother	10.1093/jac/43.6.759	1999	*
Pathogenicity	Comparison of the mycobactericidal activity of ortho- phthalaldehyde, glutaraldehyde and other dialdehydes by a quantitative suspension test.	Fraud S, Maillard JY, Russell AD	J Hosp Infect	10.1053/jhin.2001.1009	2001	*
Pathogenicity	Effects of ortho-phthalaldehyde, glutaraldehyde and chlorhexidine diacetate on Mycobacterium chelonae and Mycobacterium abscessus strains with modified permeability.	Fraud S, Hann AC, Maillard JY, Russell AD	J Antimicrob Chemother	10.1093/jac/dkg099	2003	*
Pathogenicity	Use of a new alginate film test to study the bactericidal efficacy of the high-level disinfectant ortho-phthalaldehyde.	Shackelford JC, Hanlon GW, Maillard JY	J Antimicrob Chemother	10.1093/jac/dki432	2005	*
Cultivation	Comparative study with two different enrichments in the culture media used in the disinfectant efficacy assay.	Sabagh BP, Souto Ada S, Reis LM, Silva SA, Pereira DC, Neves Mde C, Pinheiro RR, Duarte RS, Miyazaki NH, Boas MH	J Microbiol Methods	10.1016/j.mimet.2011.12.004	2011	*
Metabolism	Gene replacement in Mycobacterium chelonae: application to the construction of porin knock-out mutants.	Calado Nogueira de Moura V, Gibbs S, Jackson M	PLoS One	10.1371/journal.pone.0094951	2014	*
Genetics	Draft Genome Sequence of Mycobacterium chelonae Type Strain ATCC 35752.	Hasan NA, Davidson RM, de Moura VC, Garcia BJ, Reynolds PR, Epperson LE, Farias-Hesson E, DeGroote MA, Jackson M, Strong M	Genome Announc	10.1128/genomeA.00536-15	2015	*
Genetics	Complete Genome Sequence of Mycobacterium chelonae Type Strain CCUG 47445, a Rapidly Growing Species of Nontuberculous Mycobacteria.	Jaen-Luchoro D, Salva-Serra F, Aliaga-Lozano F, Segui C, Busquets A, Ramirez A, Ruiz M, Gomila M, Lalucat J, Bennasar-Figueras A	Genome Announc	10.1128/genomeA.00550-16	2016	*
Pathogenicity	Detection of Mycobacterium chelonae, Mycobacterium abscessus Group, and Mycobacterium fortuitum Complex by a Multiplex Real-Time PCR Directly from Clinical Samples Using the BD MAX System.	Rocchetti TT, Silbert S, Gostnell A, Kubasek C, Campos Pignatari AC, Widen R	J Mol Diagn	10.1016/j.jmoldx.2016.10.004	2017	*
Pathogenicity	Discovery of novel antimycobacterial drug therapy in biofilm of pathogenic nontuberculous mycobacterial keratitis.	Aung TT, Chor WHJ, Yam JKH, Givskov M, Yang L, Beuerman RW	Ocul Surf	10.1016/j.jtos.2017.06.002	2017	*
Phylogeny	Description of Mycobacterium chelonae subsp. bovis subsp. nov., isolated from cattle (Bos taurus coreanae), emended description of Mycobacterium chelonae and creation of Mycobacterium chelonae subsp. chelonae subsp. nov.	Kim BJ, Kim GN, Kim BR, Jeon CO, Jeong J, Lee SH, Lim JH, Lee SH, Kim CK, Kook YH, Kim BJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.002217	2017	*
Phylogeny	A description of Mycobacterium chelonae subsp. gwanakae subsp. nov., a rapidly growing mycobacterium with a smooth colony phenotype due to glycopeptidolipids.	Kim BJ, Kim BR, Jeong J, Lim JH, Park SH, Lee SH, Kim CK, Kook YH, Kim BJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.003056	2018	*
Phylogeny	Variation among human, veterinary and environmental Mycobacterium chelonae-abscessus complex isolates observed using core genome phylogenomic analysis, targeted gene comparison, and anti-microbial susceptibility patterns.	Fogelson SB, Camus AC, Lorenz WW, Vasireddy R, Vasireddy S, Smith T, Brown-Elliott BA, Wallace RJ Jr, Hasan NA, Reischl U, Sanchez S	PLoS One	10.1371/journal.pone.0214274	2019	*

References

#11257

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 43804

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#36330 ; Curators of the CIP;

#43559

Byoung-Jun Kim, Ga-Na Kim, Bo-Ram Kim, Che Ok Jeon, Joseph Jeong, Seon Ho Lee, Ji-Hun Lim, Seung-Heon Lee, Chang Ki Kim, Yoon-Hoh Kook, Bum-Joon Kim: Description of Mycobacterium chelonae subsp. bovis subsp. nov., isolated from cattle (Bos taurus coreanae), emended description of Mycobacterium chelonae and creation of Mycobacterium chelonae subsp. chelonae subsp. nov.. IJSEM 67: 3882 - 3887 2017 ( DOI 10.1099/ijsem.0.002217 , PubMed 28895525 )

#57692 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 47445

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

* These data were automatically processed and therefore are not curated

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