Mycobacterium stomatepiae (DSM 45059, CIP 109275, NCIMB 14252)

Name: Mycobacterium stomatepiae (DSM 45059, CIP 109275, NCIMB 14252)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 8541

Type strain

Strain Designation T 11, T 3

Culture col. no. DSM 45059 CIP 109275 NCIMB 14252 T 11 T 3 1 more

NCBI tax ID(s) 470076

Special Collections DSMZ JCM CIP Literature strains

Links

Mycobacterium stomatepiae T 11 is an aerobe, mesophilic, rod-shaped animal pathogen that was isolated from frozen fish tissues.

rod-shaped aerobe mesophilic animal pathogen genome sequence 16S sequence

Name and taxonomic classification

SI-ID 360284

CIP 109275,DSM 45059,NCIMB 14252,JCM 17783

@ref 20215

Last LPSN update 2025-12-16 09:48:52

Domain Bacillati

Phylum Actinomycetota

Class Actinomycetes

Order Mycobacteriales

Family Mycobacteriaceae

Genus Mycobacterium

Species Mycobacterium stomatepiae

Full scientific name Mycobacterium stomatepiae Pourahmad et al. 2008

Morphology

Cell morphology

@ref	Cell shape
31202	rod-shaped
123322
125439
125438

Pigmentation

31202

Productionyes

Multimedia

@ref:	12304
multimedia content:	DSM_45059.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_45059.jpg
caption:	Medium 645 28°C
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
12304	MIDDLEBROOK MEDIUM (DSMZ Medium 645)	Medium recipe at MediaDive	Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water
37490	MEDIUM 93 - for Mycobacterium avium subsp. sylvaticum		Distilled water make up to (900.000 ml);Glycerol (4.500 ml);Middlebrook7H10 agar (17.000 g);Middelbrook OADC enrichment (100.000 ml)
123322	CIP Medium 93	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
12304	positive	growth	28	mesophilic
31202	positive	growth	22-30
31202	positive	optimum	30	mesophilic
37490	positive	growth	30	mesophilic
67770	positive	growth	28	mesophilic

Culture pH

@ref	Ability	Type	PH
31202	positive	optimum	7
31202	positive	growth	7

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
31202	aerobe
123322	facultative anaerobe
125439	obligate aerobe

Spore formation

31202

Spore formationno

Observation

31202

Observationaggregates in chains

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68371	18305 ChEBI	arbutin	-	builds acid from	from API 50CH acid
68371	17057 ChEBI	cellobiose	-	builds acid from	from API 50CH acid
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	-	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	-	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	-	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	-	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	-	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
68371	17306 ChEBI	maltose	-	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	-	builds acid from	from API 50CH acid
123322	17632 ChEBI	nitrate	-	reduction
123322	16301 ChEBI	nitrite	-	reduction
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	-	builds acid from	from API 50CH acid
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
68371	17992 ChEBI	sucrose	-	builds acid from	from API 50CH acid
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Metabolite production

@ref	Chebi-ID	Metabolite	Production
123322	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	+	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
31202	arylsulfatase	+	3.1.6.1
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
31202	catalase	+	1.11.1.6
123322	catalase	+	1.11.1.6
68382	cystine arylamidase	+	3.4.11.3	from API zym
68382	esterase (C 4)	-		from API zym
68382	esterase lipase (C 8)	+		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
123322	oxidase	-
68382	trypsin	+	3.4.21.4	from API zym
123322	urease	+	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
123322	-	-	-	+	-	+	-	+	+	+	+	+	+	-	-	-	-	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
123322	not determinedn.d.	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+/-	-	-	-	-	-	-	+/-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+/-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2
#Host	#Fishes

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
12304	frozen fish tissues	London Zoo Aquarium	United Kingdom	GBR	Europe
67770	Granulomatous lesions in spleen tissue from a striped barombi mbo cichlid, Stomatepia mariae	London Zoo Aquarium	United Kingdom	GBR	Europe
123322	Animal, Fish, Barombi Mbo cichlid	London	United Kingdom	GBR	Europe

Interaction and safety

Risk assessment

@ref	Pathogenicity animal	Biosafety level	Biosafety level comment
12304		1	Risk group (German classification) According to TRBA 466
123322		1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM1073171v1 assembly for Mycobacterium stomatepiae JCM 17783	complete	GCA_010731715	470076.10	2990133754	470076	96.87
66792	ASM2582178v1 assembly for Mycobacterium stomatepiae DSM 45059	contig	GCA_025821785	470076.12	8113875198	470076	33.96

Genome browser: GCA_010731715

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Mycobacterium stomatepiae strain DSM 45059 16S ribosomal RNA gene and 16S-23S ribosomal RNA intergenic spacer, partial sequence	HM022202	1797		470076
12304	Mycobacterium stomatepiae partial 16S rRNA gene, type strain DSM 45059T	AM884331	1471		470076

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Mycobacterium stomatepiae strain JCM 17783
PATRIC: 470076.10

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	51.10	no
125439	motility	BacteriaNetⓘ	no	84.40	no
125439	gram_stain	BacteriaNetⓘ	positive	96.70	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	99.30	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Mycobacterium stomatepiae JCM 17783
NCBI: GCA_010731715.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	91.64	no
125438	anaerobic	anaerobicⓘ	no	97.77	no
125438	aerobic	aerobicⓘ	yes	78.73	no
125438	spore-forming	spore-formingⓘ	no	62.02	yes
125438	thermophilic	thermophileⓘ	no	89.79	yes
125438	flagellated	motile2+ⓘ	no	84.50	no

Literature

Title	Authors	Journal	DOI	Year
Prevalence of Mycobacterium lentiflavum in cystic fibrosis patients, France.	Phelippeau M, Dubus JC, Reynaud-Gaubert M, Gomez C, Stremler le Bel N, Bedotto M, Prudent E, Drancourt M.	BMC Pulm Med	10.1186/s12890-015-0123-y	2015
Microbiological features and clinical relevance of new species of the genus Mycobacterium.	Tortoli E.	Clin Microbiol Rev	10.1128/cmr.00035-14	2014
The Presence of esat-6 and cfp10 and Other Gene Orthologs of the RD 1 Region in Non-Tuberculous Mycobacteria, Mycolicibacteria, Mycobacteroides and Mycolicibacter as Possible Impediments for the Diagnosis of (Animal) Tuberculosis	Gcebe N, Hlokwe T, Bouw A, Michel A, Rutten V.	Microorganisms		2024
Mycobacterium ahvazicum sp. nov., the nineteenth species of the Mycobacterium simiae complex.	Bouam A, Heidarieh P, Shahraki AH, Pourahmad F, Mirsaeidi M, Hashemzadeh M, Baptiste E, Armstrong N, Levasseur A, Robert C, Drancourt M.	Sci Rep	10.1038/s41598-018-22526-z	2018
Mycobacterium terramassiliense, Mycobacterium rhizamassiliense and Mycobacterium numidiamassiliense sp. nov., three new Mycobacterium simiae complex species cultured from plant roots.	Bouam A, Armstrong N, Levasseur A, Drancourt M.	Sci Rep	10.1038/s41598-018-27629-1	2018
Characterization of Mycobacterium salfingeri sp. nov.: A novel nontuberculous mycobacteria isolated from a human wound infection.	Musser E, Smith C, Halse TA, Kohlerschmidt D, Rourke A, Fiero A, Musser KA, Escuyer V, Lapierre P.	Front Microbiol	10.3389/fmicb.2022.992610	2022
Mycobacterium stomatepiae sp. nov., a slowly growing, non-chromogenic species isolated from fish.	Pourahmad F, Cervellione F, Thompson KD, Taggart JB, Adams A, Richards RH	Int J Syst Evol Microbiol	10.1099/ijs.0.2008/001164-0	2008

References

#12304	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 45059
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#31202	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27529 (see below)
#37490	; Curators of the CIP;
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#123322	Collection of Institut Pasteur ; Curators of the CIP; CIP 109275
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Mycobacterium stomatepiae (DSM 45059, CIP 109275, NCIMB 14252)

Name and taxonomic classification

Morphology

Cell morphology

Pigmentation

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Observation

Metabolite utilization

Metabolite production

Enzymes

API zym

API 50CHac

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_010731715

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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