Mycobacterium nebraskense (DSM 44803, ATCC BAA-837, CIP 108973)

Name: Mycobacterium nebraskense (DSM 44803, ATCC BAA-837, CIP 108973)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 8526

Type strain

Strain Designation UNMC-MY1349

Culture col. no. DSM 44803 ATCC BAA-837 CIP 108973 CCUG 55635 UNMC-MY1349

NCBI tax ID(s) 244292

Special Collections DSMZ CCUG CIP Literature strains

History <- A. M. Mohamed, Nebraska Univ. <- NMC CIP <- 2005, DSMZ <- A. M. Mohamed, Nebraska Univ., USA <- NMC: strain UNMC-MY1349

Links

Mycobacterium nebraskense UNMC-MY1349 is a microaerophile, rod-shaped bacterium that was isolated from sputum.

rod-shaped microaerophile genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 134439

CT2025-00090,ATCC BAA-837,DSM 44803,CIP 108973,CCUG 55635

@ref 20215

Last LPSN update 2026-02-09 11:19:43

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Mycobacteriales

Family Mycobacteriaceae

Genus Mycobacterium

Species Mycobacterium nebraskense

Full scientific name Mycobacterium nebraskense Mohamed et al. 2004

BacDive ID	Other strains from **Mycobacterium nebraskense** (1)	Type strain
156187	M. nebraskense CCUG 60657

Morphology

Cell morphology

@ref	Confidence	Cell shape	Gram stain
31247		rod-shaped
121249
125438	90
125439	98.8		positive
125439	91

Pigmentation

31247

Productionyes

Multimedia

@ref:	11990
multimedia content:	DSM_44803-1.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_44803-1.jpg
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

@ref:	11990
multimedia content:	DSM_44803-2.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_44803-2.jpg
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

@ref:	11990
multimedia content:	DSM_44803.jpg
multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_44803.jpg
caption:	Medium 645 37°C
intellectual property rights:	© Leibniz-Institut DSMZ
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
11990	MIDDLEBROOK MEDIUM (DSMZ Medium 645)	Medium recipe at MediaDive	Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water
36344	MEDIUM 93 - for Mycobacterium avium subsp. sylvaticum		Distilled water make up to (900.000 ml);Glycerol (4.500 ml);Middlebrook7H10 agar (17.000 g);Middelbrook OADC enrichment (100.000 ml)
121249	CIP Medium 93	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
11990	positive	growth	37
31247	positive	growth	25-35
31247	positive	optimum	32.5
36344	positive	growth	30
60680	positive	growth	37

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
60680	microaerophile
121249	obligate aerobe
125439	obligate aerobe	99

Spore formation

31247

Spore formationno

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
121249	17632 ChEBI	nitrate	+	reduction
121249	16301 ChEBI	nitrite	-	reduction

Metabolite production

@ref	Chebi-ID	Metabolite	Production
121249	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
31247	catalase	+	1.11.1.6
121249	catalase	+	1.11.1.6
68382	cystine arylamidase	+	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
121249	oxidase	-
68382	trypsin	+	3.4.21.4	from API zym
121249	urease	-	3.5.1.5
68382	valine arylamidase	+		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
121249	-	-	+	+	-	+	+	+	+	-	+	+	-	-	-	+	+	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host Body Product	#Fluids	#Sputum

Isolation

@ref	Sample type	Sampling date	Geographic location	Country	Country ISO 3 Code	Continent
11990	sputum		State of Nebraska	USA	USA	North America
60680	Human sputum	2002	Omaha	USA	USA	North America
121249	Sputum			United States of America	USA	North America

Taxonmaps

69479

Global distribution of 16S sequence GQ153277 (>99% sequence identity) for Mycobacterium from Microbeatlas

Aquatic Samples	382
Soil Samples	881
Animal Samples	203
Plant Samples	54
Total Samples	1520

Interaction and safety

Risk assessment

@ref	Pathogenicity human	Biosafety level	Biosafety level comment
11990	yes, in single cases	1	Risk group (German classification) According to TRBA 466
121249		1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM210225v1 assembly for Mycobacterium nebraskense DSM 44803	contig	GCA_002102255	244292.7	8111202701	244292	41.15
66792	ASM2582184v1 assembly for Mycobacterium nebraskense DSM 44803	scaffold	GCA_025821845	244292.12	8111839331	244292	33.8

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Mycobacterium nebraskense strain DSM 44803 16S ribosomal RNA gene, partial sequence	DQ124112	525	244292
20218	Mycobacterium nebraskense strain ATCC BAA-837 16S ribosomal RNA gene, partial sequence	GQ153277	1495	244292
31247	Mycobacterium nebraskense 16S-23S ribosomal RNA intergenic spacer, partial sequence	AY368458	209	244292
11990	Mycobacterium nebraskense strain UNMC-MY1349 16S ribosomal RNA gene, complete sequence	AY368456	1506	244292

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Mycobacterium nebraskense DSM 44803
NCBI: GCA_025821845

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	72.90	no
125439	motility	BacteriaNetⓘ	no	91.00	no
125439	gram_stain	BacteriaNetⓘ	positive	98.80	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	99.00	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Mycobacterium nebraskense strain DSM 44803
PATRIC: 244292.7

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	87.30	no
125438	anaerobic	anaerobicⓘ	no	91.56	yes
125438	spore-forming	spore-formingⓘ	no	59.96	yes
125438	aerobic	aerobicⓘ	yes	70.83	no
125438	thermophilic	thermophileⓘ	no	95.29	yes
125438	flagellated	motile2+ⓘ	no	90.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Computational approach involving use of the internal transcribed spacer 1 region for identification of Mycobacterium species.	Mohamed AM, Kuyper DJ, Iwen PC, Ali HH, Bastola DR, Hinrichs SH.	J Clin Microbiol	10.1128/jcm.43.8.3811-3817.2005	2005
Phylogeny	Mycobacterium nebraskense sp. nov., a novel slowly growing scotochromogenic species.	Mohamed AM, Iwen PC, Tarantolo S, Hinrichs SH	Int J Syst Evol Microbiol	10.1099/ijs.0.63126-0	2004

References

#11990	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44803
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#27570	IJSEM 2057 2004 ( DOI 10.1099/ijs.0.63126-0 , PubMed 15545434 )
#31247	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27570
#36344	; Curators of the CIP;
#60680	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 55635
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#121249	Collection of Institut Pasteur ; Curators of the CIP; CIP 108973
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Mycobacterium nebraskense (DSM 44803, ATCC BAA-837, CIP 108973)

Name and taxonomic classification

Morphology

Cell morphology

Pigmentation

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Metabolite utilization

Metabolite production

Enzymes

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_002102255

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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