Name: Mycobacterium intracellulare subsp. intracellulare 3600
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 8403

Type strain:

Species: Mycobacterium intracellulare subsp. intracellulare

Strain Designation: 3600

Culture col. no.: DSM 43223, ATCC 13950, ATCC 15985, JCM 6384, NCTC 10682, CCUG 28005, NCTC 13025, TMC 1406, CIP 104243, SN 424, KCTC 9514

Strain history: M. Tsukamura 13085 <-- ATCC 13950 <-- R. E. Gordon 3600 <-- D. Smith (J. T. Cuttino and A. M. McCabe's original strain).

NCBI tax ID(s): 1767 (species)

Section

Mycobacterium intracellulare subsp. intracellulare 3600 is a microaerophile, mesophilic human pathogen that was isolated from Human lymph node.

human pathogen
mesophilic
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Mycobacteriales*
	Family	*Mycobacteriaceae*
	Genus	*Mycobacterium*
	Species	**Mycobacterium intracellulare subsp. intracellulare**
	Full Scientific Name (PNU)	Mycobacterium intracellulare subsp. intracellulare (Cuttino and McCabe 1949) Castejon et al. 2018

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #69480]	Motility	no Confidence in %92.229

[Ref.: #49062]

Incubation period

6 days

Multimedia content

[Ref.: #10880]

Caption:

Medium 645 37°C

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Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

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Culture medium

MIDDLEBROOK MEDIUM (DSMZ Medium 645)

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Culture medium growth

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Culture medium link

Medium recipe provided by DSMZ

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Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #10880]			growth	37 °C
[Ref.: #67770]			growth	37 °C

[Ref.: #10880]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #49062]

Oxygen tolerance

microaerophile

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.956

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #67770]	Sample type/isolated from	Human lymph node
* marker position based on {}

[Ref.: #69479]

Global distribution of 16S sequence JF271818 (>99% sequence identity) for Mycobacterium from Microbeatlas

Aquatic Samples	4789
Soil Samples	12779
Animal Samples	3677
Plant Samples	2833
Total Samples	24078

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #10880]	Pathogenicity (human)	yes
[Ref.: #10880]	Pathogenicity (animal)	yes
[Ref.: #10880]	Biosafety level	2	Risk group (German classification) According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Mycobacterium intracellulare ATCC 13950 16S ribosomal RNA gene, partial sequence	GQ153276	1495	ENA	487521 tax ID	*
[Ref.: #20218]	Mycobacterium intracellulare ATCC 13950 16S ribosomal RNA gene, partial sequence	JF271818	1395	ENA	487521 tax ID	*
[Ref.: #20218]	Mycobacterium intracellulare ATCC 13950 16S-23S ribosomal RNA intergenic spacer, partial sequence	JQ411532	280	ENA	487521 tax ID	*
[Ref.: #20218]	Mycobacterium intracellulare sequevar Min-A partial 16S ribosomal RNA gene, partial 23S ribosomal RNA gene, and complete internal transcribed spacer	L07859	309	ENA	1767 tax ID	*
[Ref.: #20218]	Mycobacterium intracellulare strain ATCC 15985 16S ribosomal RNA gene and 16S-23S ribosomal RNA intergenic spacer, partial sequence	EF521903	438	ENA	1767 tax ID	*
[Ref.: #20218]	M.intracellulare 16S ribosomal RNA gene	M59276	146	ENA	1767 tax ID	*
[Ref.: #20218]	Mycobacterium intracellulare 16S rRNA gene	X52927	1452	ENA	1767 tax ID	*
[Ref.: #20218]	Mycobacterium intracellulare CCUG:28005 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	HM584723	183	ENA	1767 tax ID	*
[Ref.: #20218]	Mycobacterium intracellulare strain CIP 104243 16S ribosomal RNA gene, partial sequence	AF547939	554	ENA	1767 tax ID	*
[Ref.: #20218]	Mycobacterium intracellulare 16S rRNA gene	AJ536036	1440	ENA	1767 tax ID	*
[Ref.: #20218]	Mycobacterium intracellulare DNA for 16S rRNA, internal transcribed spacer, 23S rRNA	AB026691	438	ENA	1767 tax ID	*
[Ref.: #20218]	M.intracellulare 16S-23S rDNA internal transcribed spacer	X74057	279	ENA	1767 tax ID	*
[Ref.: #20218]	M.intracellulare leader region of 16S rRNA gene	X74063	191	ENA	1767 tax ID	*
[Ref.: #20218]	Mycobacterium intracellulare strain ATCC13950 16S ribosomal RNA (rrs) gene, partial sequence	AF059849	173	ENA	487521 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Mycobacterium intracellulare ATCC 13950 ATCC 13950	GCA_000172115	contig	GenBank	487521 tax ID
[Ref.: #66792]	Mycobacterium intracellulare ATCC 13950 ATCC 13950	GCA_000277125	complete	GenBank	487521 tax ID	*
[Ref.: #66792]	Mycobacterium intracellulare ATCC 13950	487521.5	wgs	PATRIC	487521 tax ID	*
[Ref.: #66792]	Mycobacterium intracellulare ATCC 13950	487521.10	complete	PATRIC	487521 tax ID	*
[Ref.: #66792]	Mycobacterium intracellulare ATCC 13950	2519103109	complete	JGI IMG/M	487521 tax ID	*
[Ref.: #66792]	Mycobacterium intracellulare ATCC 13950	645058739	draft	JGI IMG/M	487521 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Mycobacterium intracellulare ATCC 13950 ATCC 13950 NCBI: GCA_000277125.1
[Ref.: #69480]	gram-positive	yes	100	yes
[Ref.: #69480]	spore-forming	no	99.956	yes
[Ref.: #69480]	anaerobic	no	99.432	no
[Ref.: #69480]	thermophile	no	98.147	no
[Ref.: #69480]	motile	no	92.229	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Mycobacterium intracellulare ATCC 13950 ATCC 13950 NCBI: GCA_000277125.1
[Ref.: #69481]	spore-forming	no	100	no

Availability in culture collections External links

[Ref.: #10880]

Culture collection no.

DSM 43223, ATCC 13950, ATCC 15985, JCM 6384, NCTC 10682, CCUG 28005, NCTC 13025, TMC 1406, CIP 104243, SN 424, KCTC 9514

[Ref.: #20218]

Associated Passport(s) in StrainInfo

153020, 97238, 153019, 153021, 153022

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Comparative genomic analysis of Mycobacterium intracellulare: implications for clinical taxonomic classification in pulmonary Mycobacterium avium-intracellulare complex disease.	Tateishi Y, Ozeki Y, Nishiyama A, Miki M, Maekura R, Fukushima Y, Nakajima C, Suzuki Y, Matsumoto S	BMC Microbiol	10.1186/s12866-021-02163-9	2021	*
Genetics	Genome-wide identification of essential genes in Mycobacterium intracellulare by transposon sequencing - Implication for metabolic remodeling.	Tateishi Y, Minato Y, Baughn AD, Ohnishi H, Nishiyama A, Ozeki Y, Matsumoto S	Sci Rep	10.1038/s41598-020-62287-2	2020	*
Phylogeny	A definition of the Mycobacterium avium complex for taxonomical and clinical purposes, a review.	van Ingen J, Turenne CY, Tortoli E, Wallace RJ Jr, Brown-Elliott BA	Int J Syst Evol Microbiol	10.1099/ijsem.0.003026	2018	*
Genetics	Genome sequence of Mycobacterium yongonense RT 955-2015 isolate from a patient misdiagnosed with multidrug-resistant tuberculosis: First clinical detection in Tanzania.	Mnyambwa NP, Kim DJ, Ngadaya E, Chun J, Ha SM, Petrucka P, Addo KK, Kazwala RR, Mfinanga SG	Int J Infect Dis	10.1016/j.ijid.2018.04.796	2018	*
Phylogeny	Mycobacterium paraintracellulare sp. nov., for the genotype INT-1 of Mycobacterium intracellulare.	Lee SY, Kim BJ, Kim H, Won YS, Jeon CO, Jeong J, Lee SH, Lim JH, Lee SH, Kim CK, Kook YH, Kim BJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.001158	2016	*
Genetics	Molecular Taxonomic Evidence for Two Distinct Genotypes of Mycobacterium yongonense via Genome-Based Phylogenetic Analysis.	Kim BJ, Kim BR, Lee SY, Kim GN, Kook YH, Kim BJ	PLoS One	10.1371/journal.pone.0152703	2016	*
Genetics	Comparative analyses of nonpathogenic, opportunistic, and totally pathogenic mycobacteria reveal genomic and biochemical variabilities and highlight the survival attributes of Mycobacterium tuberculosis.	Rahman SA, Singh Y, Kohli S, Ahmad J, Ehtesham NZ, Tyagi AK, Hasnain SE	mBio	10.1128/mBio.02020-14	2014	*
Genetics	Complete genome sequence of Mycobacterium intracellulare strain ATCC 13950(T).	Kim BJ, Choi BS, Lim JS, Choi IY, Lee JH, Chun J, Kook YH, Kim BJ	J Bacteriol	10.1128/JB.00295-12	2012	*
Phylogeny	Mycobacterium yongonense sp. nov., a slow-growing non-chromogenic species closely related to Mycobacterium intracellulare.	Kim BJ, Math RK, Jeon CO, Yu HK, Park YG, Kook YH, Kim BJ	Int J Syst Evol Microbiol	10.1099/ijs.0.037465-0	2012	*
Phylogeny	Variable-number tandem-repeat markers for typing Mycobacterium intracellulare strains isolated in humans.	Dauchy FA, Degrange S, Charron A, Dupon M, Xin Y, Bebear C, Maugein J	BMC Microbiol	10.1186/1471-2180-10-93	2010	*
Pathogenicity	Antitubercular agents. Part 1: synthesis of phthalimido- and naphthalimido-linked phenazines as new prototype antitubercular agents.	Kamal A, Hari Babu A, Venkata Ramana A, Sinha R, Yadav JS, Arora SK	Bioorg Med Chem Lett	10.1016/j.bmcl.2005.01.085	2005	*
Enzymology	Cloning, sequencing and expression of the gene for alpha antigen from Mycobacterium intracellulare and use of PCR for the rapid identification of Mycobacterium intracellulare.	Kitaura H, Ohara N, Matsuo T, Tasaka H, Kobayashi K, Yamada T	Biochem Biophys Res Commun	10.1006/bbrc.1993.2417	1993	*
Pathogenicity	Further studies on colistin (polymyxin E)-induced cell leakage in mycobacteria: Mg++ efflux in Mycobacterium avium and its effects on drug-susceptibility.	Rastogi N, Potar MC, Henrotte JG, Franck G, David HL	Zentralbl Bakteriol Mikrobiol Hyg A	10.1016/s0176-6724(88)80010-5	1988	*
	Feature of Adhesins Produced by Human Clinical Isolates of Mycobacterium intracellulare, Mycobacterium intracellulare subsp. chimaera and Closely Related Species.	Lefrancois LH, Cochard T, Branger M, Peuchant O, Conde C, Pastuszka A, Locht C, Lanotte P, Biet F	Microorganisms	10.3390/microorganisms8081154	2020	*

References

#10880

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 43223

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#49062 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 28005

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

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Mycobacterium intracellulare ATCC 13950 ATCC 13950

Mycobacterium intracellulare ATCC 13950 ATCC 13950

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