Name: Mycobacterium intracellulare 3600
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 8403

Type strain:

Species: Mycobacterium intracellulare

Strain Designation: 3600, 140310001

Culture col. no.: DSM 43223, ATCC 13950, ATCC 15985, JCM 6384, NCTC 10682, CCUG 28005, NCTC 13025, TMC 1406, CIP 104243, SN 424, KCTC 9514

Strain history: CIP <- 1994, V. Vincent, Inst. Pasteur, Paris, France: strain 14 031 0001 <- 1966, ATCC <- R.E. Gordon <- D. Smith (Cuttino and McCabe's original strain)

NCBI tax ID(s): 487521 (strain), 1767 (species)

SI-ID 92812

ATCC 13950, CCUG 28005, CIP 104243, ATCC 15985, DSM 43223, JCM 6384, NCTC 10682, NCTC 43223, KCTC 9514, NCTC 13025

Special Collections

Other strains from Mycobacterium intracellulare

Section

Mycobacterium intracellulare 3600 is a microaerophile, Gram-positive human pathogen that was isolated from Human lymph node.

Gram-positive
human pathogen
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Mycobacteriales*
	Family	*Mycobacteriaceae*
	Genus	*Mycobacterium*
	Species	**Mycobacterium intracellulare**
	Full Scientific Name (LPSN)	Mycobacterium intracellulare corrig. (Cuttino and McCabe 1949) Runyon 1965 (Approved Lists 1980)

	Synonym	Mycobacterium intracellularis
	Synonym	Mycobacterium paraintracellulare
	Synonym	"Nocardia* intracellularis"*

Information on morphological and physiological properties Morphology

[Ref.: #120272]	Cell shape	rod-shaped

[Ref.: #120272]	Motility	no

[Ref.: #49062]

Incubation period

6 days

Multimedia content

[Ref.: #10880]

Caption:

Medium 645 37°C

[Ref.: #10880]

Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

[Ref.: #10880]

Culture medium

MIDDLEBROOK MEDIUM (DSMZ Medium 645)

[Ref.: #10880]

Culture medium growth

[Ref.: #10880]

Culture medium link

Medium recipe at MediaDive

[Ref.: #10880]

Culture medium composition

expand / minimize

[Ref.: #120272]

Culture medium

CIP Medium 267

[Ref.: #120272]

Culture medium growth

[Ref.: #120272]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #10880]		growth	37 °C
[Ref.: #67770]		growth	37 °C
[Ref.: #120272]		growth	22-45 °C
[Ref.: #120272]	no	growth	10 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #49062]	Oxygen tolerance	microaerophile
[Ref.: #120272]	Oxygen tolerance	obligate aerobe

[Ref.: #69481]

Ability of spore formation

no Confidence in %100

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #120272]	hippurate ChEBI	-	hydrolysis
[Ref.: #120272]	nitrate ChEBI	+	reduction
[Ref.: #120272]	nitrate ChEBI	-	respiration
[Ref.: #120272]	nitrite ChEBI	-	reduction

	Metabolite production	Metabolite	Production
[Ref.: #120272]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #120272]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #120272]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	+	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #120272]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #120272]	gelatinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #120272]	oxidase	-
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #120272]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #120272]	7855	-	+	+	+	-	+	-	+	+	-	+	+	-	-	-	-	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #67770]	Sample type/isolated from	Human lymph node

[Ref.: #120272]	Geographic location (country and/or sea, region)	North Carolina
[Ref.: #120272]	Country	United States of America
[Ref.: #120272]	Country ISO 3 Code	USA
[Ref.: #120272]	Continent	North America
[Ref.: #120272]	Isolation date	1949
* marker position based on {}

[Ref.: #69479]

Global distribution of 16S sequence JF271818 (>99% sequence identity) for Mycobacterium from Microbeatlas

Aquatic Samples	4789
Soil Samples	12779
Animal Samples	3677
Plant Samples	2833
Total Samples	24078

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #10880]	Pathogenicity (human)	yes
[Ref.: #10880]	Pathogenicity (animal)	yes
[Ref.: #10880]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #120272]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Mycobacterium intracellulare ATCC 13950 16S ribosomal RNA gene, partial sequence	GQ153276	1495	GenBank ENA	487521 tax ID	*
[Ref.: #20218]	Mycobacterium intracellulare ATCC 13950 16S ribosomal RNA gene, partial sequence	JF271818	1395	GenBank ENA	487521 tax ID	*
[Ref.: #20218]	Mycobacterium intracellulare ATCC 13950 16S-23S ribosomal RNA intergenic spacer, partial sequence	JQ411532	280	GenBank ENA	487521 tax ID	*
[Ref.: #20218]	Mycobacterium intracellulare sequevar Min-A partial 16S ribosomal RNA gene, partial 23S ribosomal RNA gene, and complete internal transcribed spacer	L07859	309	GenBank ENA	1767 tax ID	*
[Ref.: #20218]	Mycobacterium intracellulare strain ATCC 15985 16S ribosomal RNA gene and 16S-23S ribosomal RNA intergenic spacer, partial sequence	EF521903	438	GenBank ENA	1767 tax ID	*
[Ref.: #20218]	M.intracellulare 16S ribosomal RNA gene	M59276	146	GenBank ENA	1767 tax ID	*
[Ref.: #20218]	Mycobacterium intracellulare 16S rRNA gene	X52927	1452	GenBank ENA	1767 tax ID	*
[Ref.: #20218]	Mycobacterium intracellulare CCUG:28005 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	HM584723	183	GenBank ENA	1767 tax ID	*
[Ref.: #20218]	Mycobacterium intracellulare strain CIP 104243 16S ribosomal RNA gene, partial sequence	AF547939	554	GenBank ENA	1767 tax ID	*
[Ref.: #20218]	Mycobacterium intracellulare 16S rRNA gene	AJ536036	1440	GenBank ENA	1767 tax ID	*
[Ref.: #20218]	Mycobacterium intracellulare DNA for 16S rRNA, internal transcribed spacer, 23S rRNA	AB026691	438	GenBank ENA	1767 tax ID	*
[Ref.: #20218]	M.intracellulare 16S-23S rDNA internal transcribed spacer	X74057	279	GenBank ENA	1767 tax ID	*
[Ref.: #20218]	M.intracellulare leader region of 16S rRNA gene	X74063	191	GenBank ENA	1767 tax ID	*
[Ref.: #124043]	Mycobacterium intracellulare ATCC 13950 DNA containing 16S-23S intergenic spacer region, strain KM05-111	LR031427	411	GenBank ENA	487521 tax ID	*
[Ref.: #124043]	Mycobacterium intracellulare ATCC 13950 DNA containing 16S-23S intergenic spacer region, strain KM05-173	LR031428	410	GenBank ENA	487521 tax ID	*
[Ref.: #124043]	Mycobacterium intracellulare ATCC 13950 DNA containing 16S-23S intergenic spacer region, strain KM06-08	LR031429	411	GenBank ENA	487521 tax ID	*
[Ref.: #124043]	Mycobacterium intracellulare ATCC 13950 DNA containing 16S-23S intergenic spacer region, strain KM06-472	LR031430	410	GenBank ENA	487521 tax ID	*
[Ref.: #124043]	Mycobacterium intracellulare ATCC 13950 16S-23S ribosomal RNA intergenic spacer, partial sequence.	JQ411526	281	GenBank ENA	1069222 tax ID	*
[Ref.: #124043]	Mycobacterium intracellulare ATCC 13950 16S-23S ribosomal RNA intergenic spacer, partial sequence.	JQ411527	280	GenBank ENA	1069223 tax ID	*
[Ref.: #124043]	Mycobacterium intracellulare ATCC 13950 16S-23S ribosomal RNA intergenic spacer, partial sequence.	JQ411528	280	GenBank ENA	1069224 tax ID	*
[Ref.: #124043]	Mycobacterium intracellulare ATCC 13950 16S-23S ribosomal RNA intergenic spacer, partial sequence.	JQ411529	280	GenBank ENA	1069225 tax ID	*
[Ref.: #20218]	Mycobacterium intracellulare strain ATCC13950 16S ribosomal RNA (rrs) gene, partial sequence	AF059849	173	GenBank ENA	487521 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Mycobacterium intracellulare ATCC 13950	GCA_000172115	contig	GenBank ENA	487521 tax ID
[Ref.: #66792]	Mycobacterium intracellulare ATCC 13950	GCA_000277125	complete	GenBank ENA	487521 tax ID	*
[Ref.: #66792]	Mycobacterium intracellulare ATCC 13950	487521.5	wgs	PATRIC	487521 tax ID	*
[Ref.: #66792]	Mycobacterium intracellulare ATCC 13950	487521.10	complete	PATRIC	487521 tax ID	*
[Ref.: #66792]	Mycobacterium intracellulare ATCC 13950	2519103109	complete	JGI IMG/M	487521 tax ID	*
[Ref.: #66792]	Mycobacterium intracellulare ATCC 13950	645058739	draft	JGI IMG/M	487521 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Mycobacterium intracellulare ATCC 13950 ATCC 13950 NCBI: GCA_000277125.1
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Mycobacterium intracellulare ATCC 13950 ATCC 13950 NCBI: GCA_000277125.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	89.958	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	96.294	yes
[Ref.: #69480]	aerobic	aerobicⓘ	yes	80.187	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	64.288	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	96.176	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	89	no

Availability in culture collections External links

[Ref.: #10880]

Culture collection no.

DSM 43223, ATCC 13950, ATCC 15985, JCM 6384, NCTC 10682, CCUG 28005, NCTC 13025, TMC 1406, CIP 104243, SN 424, KCTC 9514

[Ref.: #77802]

SI-ID 92812

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Comparative genomic analysis of Mycobacterium intracellulare: implications for clinical taxonomic classification in pulmonary Mycobacterium avium-intracellulare complex disease.	Tateishi Y, Ozeki Y, Nishiyama A, Miki M, Maekura R, Fukushima Y, Nakajima C, Suzuki Y, Matsumoto S	BMC Microbiol	10.1186/s12866-021-02163-9	2021	*
Genetics	Genome-wide identification of essential genes in Mycobacterium intracellulare by transposon sequencing - Implication for metabolic remodeling.	Tateishi Y, Minato Y, Baughn AD, Ohnishi H, Nishiyama A, Ozeki Y, Matsumoto S	Sci Rep	10.1038/s41598-020-62287-2	2020	*
Phylogeny	A definition of the Mycobacterium avium complex for taxonomical and clinical purposes, a review.	van Ingen J, Turenne CY, Tortoli E, Wallace RJ Jr, Brown-Elliott BA	Int J Syst Evol Microbiol	10.1099/ijsem.0.003026	2018	*
Genetics	Genome sequence of Mycobacterium yongonense RT 955-2015 isolate from a patient misdiagnosed with multidrug-resistant tuberculosis: First clinical detection in Tanzania.	Mnyambwa NP, Kim DJ, Ngadaya E, Chun J, Ha SM, Petrucka P, Addo KK, Kazwala RR, Mfinanga SG	Int J Infect Dis	10.1016/j.ijid.2018.04.796	2018	*
Phylogeny	Mycobacterium paraintracellulare sp. nov., for the genotype INT-1 of Mycobacterium intracellulare.	Lee SY, Kim BJ, Kim H, Won YS, Jeon CO, Jeong J, Lee SH, Lim JH, Lee SH, Kim CK, Kook YH, Kim BJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.001158	2016	*
Genetics	Molecular Taxonomic Evidence for Two Distinct Genotypes of Mycobacterium yongonense via Genome-Based Phylogenetic Analysis.	Kim BJ, Kim BR, Lee SY, Kim GN, Kook YH, Kim BJ	PLoS One	10.1371/journal.pone.0152703	2016	*
Genetics	Comparative analyses of nonpathogenic, opportunistic, and totally pathogenic mycobacteria reveal genomic and biochemical variabilities and highlight the survival attributes of Mycobacterium tuberculosis.	Rahman SA, Singh Y, Kohli S, Ahmad J, Ehtesham NZ, Tyagi AK, Hasnain SE	mBio	10.1128/mBio.02020-14	2014	*
Genetics	Complete genome sequence of Mycobacterium intracellulare strain ATCC 13950(T).	Kim BJ, Choi BS, Lim JS, Choi IY, Lee JH, Chun J, Kook YH, Kim BJ	J Bacteriol	10.1128/JB.00295-12	2012	*
Phylogeny	Mycobacterium yongonense sp. nov., a slow-growing non-chromogenic species closely related to Mycobacterium intracellulare.	Kim BJ, Math RK, Jeon CO, Yu HK, Park YG, Kook YH, Kim BJ	Int J Syst Evol Microbiol	10.1099/ijs.0.037465-0	2012	*
Phylogeny	Variable-number tandem-repeat markers for typing Mycobacterium intracellulare strains isolated in humans.	Dauchy FA, Degrange S, Charron A, Dupon M, Xin Y, Bebear C, Maugein J	BMC Microbiol	10.1186/1471-2180-10-93	2010	*
Pathogenicity	Antitubercular agents. Part 1: synthesis of phthalimido- and naphthalimido-linked phenazines as new prototype antitubercular agents.	Kamal A, Hari Babu A, Venkata Ramana A, Sinha R, Yadav JS, Arora SK	Bioorg Med Chem Lett	10.1016/j.bmcl.2005.01.085	2005	*
Enzymology	Cloning, sequencing and expression of the gene for alpha antigen from Mycobacterium intracellulare and use of PCR for the rapid identification of Mycobacterium intracellulare.	Kitaura H, Ohara N, Matsuo T, Tasaka H, Kobayashi K, Yamada T	Biochem Biophys Res Commun	10.1006/bbrc.1993.2417	1993	*
Pathogenicity	Further studies on colistin (polymyxin E)-induced cell leakage in mycobacteria: Mg++ efflux in Mycobacterium avium and its effects on drug-susceptibility.	Rastogi N, Potar MC, Henrotte JG, Franck G, David HL	Zentralbl Bakteriol Mikrobiol Hyg A	10.1016/s0176-6724(88)80010-5	1988	*
	Feature of Adhesins Produced by Human Clinical Isolates of Mycobacterium intracellulare, Mycobacterium intracellulare subsp. chimaera and Closely Related Species.	Lefrancois LH, Cochard T, Branger M, Peuchant O, Conde C, Pastuszka A, Locht C, Lanotte P, Biet F	Microorganisms	10.3390/microorganisms8081154	2020	*

References

#10880

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 43223

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#49062 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 28005

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#77802

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID92812.1 )

#120272 Collection of Institut Pasteur ; Curators of the CIP; CIP 104243

#124043

Dr. Isabel Schober, Dr. Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Mycobacterium intracellulare subsp. intracellulare in external resources:
SILVA
BRENDA
PANGAEA
GBIF

Mycobacterium intracellulare ATCC 13950 ATCC 13950

Mycobacterium intracellulare ATCC 13950 ATCC 13950

Change proposal

Change proposal