Bacillus smithii (DSM 459, CCUG 26018, LMG 6327)

Name: Bacillus smithii (DSM 459, CCUG 26018, LMG 6327)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 810

Type strain

Strain Designation E 28-66

Culture col. no. DSM 459 CCUG 26018 LMG 6327 NRRL B-14311 E 28-66

NCBI tax ID(s) 1479

Special Collections DSMZ CCUG

History <- F. Hollaus, E 28-66 (Bacillus coagulans)

Links

Bacillus smithii E 28-66 is a bacterium that was isolated from sugar beet juice from extraction installations.

Bacteria

Name and taxonomic classification

SI-ID 13588

LMG 6327,DSM 459,LMG 12527,NRRL B-14311,CCUG 26018

@ref 20215

Last LPSN update 2026-02-09 11:17:57

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Bacillaceae

Genus Bacillus

Species Bacillus smithii

Full scientific name Bacillus smithii Nakamura et al. 1988

BacDive ID	Other strains from **Bacillus smithii** (5)	Type strain
815	B. smithii NRS-173, DSM 4216, IFO 15311, NBRC 15311, ... (type strain)
811	B. smithii E 30-66, DSM 460
812	B. smithii 35-66, DSM 2319
813	B. smithii E 45-66, DSM 2320
814	B. smithii E 50-67, DSM 2321

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
222	NUTRIENT AGAR (DSMZ Medium 1)		Medium recipe at MediaDive	Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
222	positive	growth	55
48342	positive	growth
48342	positive	growth	42
48342	positive	growth	56

Physiology and metabolism

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	methylglyoxal degradation	100	5 of 5
66794	denitrification	100	2 of 2
66794	sulfopterin metabolism	100	4 of 4
66794	coenzyme A metabolism	100	4 of 4
66794	vitamin K metabolism	100	5 of 5
66794	palmitate biosynthesis	100	22 of 22
66794	ribulose monophosphate pathway	100	2 of 2
66794	L-lactaldehyde degradation	100	3 of 3
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	valine metabolism	100	9 of 9
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	adipate degradation	100	2 of 2
66794	taurine degradation	100	1 of 1
66794	folate polyglutamylation	100	1 of 1
66794	aerobactin biosynthesis	100	1 of 1
66794	suberin monomers biosynthesis	100	2 of 2
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	tetrahydrofolate metabolism	92.86	13 of 14
66794	vitamin B1 metabolism	92.31	12 of 13
66794	pentose phosphate pathway	90.91	10 of 11
66794	propionate fermentation	90	9 of 10
66794	alanine metabolism	89.66	26 of 29
66794	molybdenum cofactor biosynthesis	88.89	8 of 9
66794	chorismate metabolism	88.89	8 of 9
66794	aspartate and asparagine metabolism	88.89	8 of 9
66794	gluconeogenesis	87.5	7 of 8
66794	C4 and CAM-carbon fixation	87.5	7 of 8
66794	isoleucine metabolism	87.5	7 of 8
66794	propanol degradation	85.71	6 of 7
66794	heme metabolism	85.71	12 of 14
66794	reductive acetyl coenzyme A pathway	85.71	6 of 7
66794	methionine metabolism	84.62	22 of 26
66794	leucine metabolism	84.62	11 of 13
66794	glutamate and glutamine metabolism	82.14	23 of 28
66794	flavin biosynthesis	80	12 of 15
66794	threonine metabolism	80	8 of 10
66794	peptidoglycan biosynthesis	80	12 of 15
66794	pyrimidine metabolism	80	36 of 45
66794	glycogen metabolism	80	4 of 5
66794	metabolism of amino sugars and derivatives	80	4 of 5
66794	cellulose degradation	80	4 of 5
66794	citric acid cycle	78.57	11 of 14
66794	photosynthesis	78.57	11 of 14
66794	allantoin degradation	77.78	7 of 9
66794	CO2 fixation in Crenarchaeota	77.78	7 of 9
66794	purine metabolism	76.6	72 of 94
66794	ppGpp biosynthesis	75	3 of 4
66794	biotin biosynthesis	75	3 of 4
66794	acetate fermentation	75	3 of 4
66794	ketogluconate metabolism	75	6 of 8
66794	glycogen biosynthesis	75	3 of 4
66794	phenol degradation	75	15 of 20
66794	degradation of sugar alcohols	75	12 of 16
66794	CMP-KDO biosynthesis	75	3 of 4
66794	tryptophan metabolism	73.68	28 of 38
66794	proline metabolism	72.73	8 of 11
66794	NAD metabolism	72.22	13 of 18
66794	cardiolipin biosynthesis	71.43	5 of 7
66794	non-pathway related	71.05	27 of 38
66794	glycolysis	70.59	12 of 17
66794	phenylalanine metabolism	69.23	9 of 13
66794	urea cycle	69.23	9 of 13
66794	lysine metabolism	69.05	29 of 42
66794	glycolate and glyoxylate degradation	66.67	4 of 6
66794	acetoin degradation	66.67	2 of 3
66794	octane oxidation	66.67	2 of 3
66794	enterobactin biosynthesis	66.67	2 of 3
66794	cyanate degradation	66.67	2 of 3
66794	formaldehyde oxidation	66.67	2 of 3
66794	acetyl CoA biosynthesis	66.67	2 of 3
66794	serine metabolism	66.67	6 of 9
66794	oxidative phosphorylation	62.64	57 of 91
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	62.5	5 of 8
66794	isoprenoid biosynthesis	61.54	16 of 26
66794	lipoate biosynthesis	60	3 of 5
66794	myo-inositol biosynthesis	60	6 of 10
66794	3-chlorocatechol degradation	60	3 of 5
66794	glycine betaine biosynthesis	60	3 of 5
66794	factor 420 biosynthesis	60	3 of 5
66794	starch degradation	60	6 of 10
66794	arginine metabolism	58.33	14 of 24
66794	degradation of pentoses	57.14	16 of 28
66794	d-mannose degradation	55.56	5 of 9
66794	histidine metabolism	55.17	16 of 29
66794	pantothenate biosynthesis	50	3 of 6
66794	toluene degradation	50	2 of 4
66794	androgen and estrogen metabolism	50	8 of 16
66794	Entner Doudoroff pathway	50	5 of 10
66794	degradation of hexoses	50	9 of 18
66794	4-hydroxyphenylacetate degradation	50	5 of 10
66794	cysteine metabolism	50	9 of 18
66794	degradation of aromatic, nitrogen containing compounds	50	6 of 12
66794	ethanol fermentation	50	1 of 2
66794	lipid metabolism	48.39	15 of 31
66794	sulfate reduction	46.15	6 of 13
66794	vitamin B6 metabolism	45.45	5 of 11
66794	nitrate assimilation	44.44	4 of 9
66794	polyamine pathway	43.48	10 of 23
66794	ubiquinone biosynthesis	42.86	3 of 7
66794	coenzyme M biosynthesis	40	4 of 10
66794	glycine metabolism	40	4 of 10
66794	phenylacetate degradation (aerobic)	40	2 of 5
66794	gallate degradation	40	2 of 5
66794	arachidonate biosynthesis	40	2 of 5
66794	3-phenylpropionate degradation	40	6 of 15
66794	phenylpropanoid biosynthesis	38.46	5 of 13
66794	d-xylose degradation	36.36	4 of 11
66794	metabolism of disaccharids	36.36	4 of 11
66794	ascorbate metabolism	36.36	8 of 22
66794	glutathione metabolism	35.71	5 of 14
66794	IAA biosynthesis	33.33	1 of 3
66794	selenocysteine biosynthesis	33.33	2 of 6
66794	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
66794	lipid A biosynthesis	33.33	3 of 9
66794	bile acid biosynthesis, neutral pathway	29.41	5 of 17
66794	tyrosine metabolism	28.57	4 of 14
66794	arachidonic acid metabolism	27.78	5 of 18
66794	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
66794	lactate fermentation	25	1 of 4
66794	cyclohexanol degradation	25	1 of 4
66794	butanoate fermentation	25	1 of 4
66794	dTDPLrhamnose biosynthesis	25	2 of 8

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Root (Rhizome)
#Host Body Product	#Plant	#Juice (natural)

Isolation

@ref	Sample type	Country	Country ISO 3 Code	Continent
222	sugar beet juice from extraction installations	Austria	AUT	Europe
48342	Sugar beet juice,extraction installations	Austria	AUT	Europe

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
222	1	Risk group (German classification) According to TRBA 466

Sequence information

GC content

222

GC-content (mol%)38.9

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Isolation and screening of thermophilic bacilli from compost for electrotransformation and fermentation: characterization of Bacillus smithii ET 138 as a new biocatalyst.	Bosma EF, van de Weijer AH, Daas MJ, van der Oost J, de Vos WM, van Kranenburg R.	Appl Environ Microbiol	10.1128/aem.03640-14	2015
	Pilot market surveillance of GMM contaminations in alpha-amylase food enzyme products: A detection strategy strengthened by a newly developed qPCR method targeting a GM Bacillus licheniformis producing alpha-amylase.	Fraiture MA, Gobbo A, Guillitte C, Marchesi U, Verginelli D, De Greve J, D'aes J, Vanneste K, Papazova N, Roosens NHC.	Food Chem (Oxf)	10.1016/j.fochms.2023.100186	2024
Biotechnology	Identification of an unauthorized genetically modified bacteria in food enzyme through whole-genome sequencing.	Fraiture MA, Bogaerts B, Winand R, Deckers M, Papazova N, Vanneste K, De Keersmaecker SCJ, Roosens NHC.	Sci Rep	10.1038/s41598-020-63987-5	2020

References

#222	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 459
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#48342	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 26018
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Bacillus smithii (DSM 459, CCUG 26018, LMG 6327)

Name and taxonomic classification

Morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Pathway annotation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

GC content

Genome-based predictions

Literature

Literature

References

BacDive

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