Name: Acinetobacter johnsonii 3865/60
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 8088

Type strain:

Species: Acinetobacter johnsonii

Strain Designation: 3865/60

Culture col. no.: DSM 6963, ATCC 17909, CIP 64.6, NCTC 10308, CCUG 19095, NCTC 12154

Strain history: 1964, W. Mannheim, Hyg. Inst., Marburg, Germany: strain 3865/60, "Achromobacter metalcaligenes"

NCBI tax ID(s): 1217663 (strain), 40214 (species)

Other strains from Acinetobacter johnsonii

Section

Acinetobacter johnsonii 3865/60 is an aerobe, mesophilic bacterium that was isolated from human duodenum.

aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Pseudomonadales*
	Family	*Moraxellaceae*
	Genus	*Acinetobacter*
	Species	**Acinetobacter johnsonii**
	Full Scientific Name (PNU)	Acinetobacter johnsonii Bouvet and Grimont 1986

Information on morphological and physiological properties Morphology

[Ref.: #2924]	Type of hemolysis	gamma
[Ref.: #2924]	Incubation period	1-2 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #2924]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #2924]

Culture medium growth

[Ref.: #2924]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #2924]

Culture medium composition

expand / minimize

[Ref.: #42154]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #42154]

Culture medium growth

[Ref.: #42154]

Culture medium composition

expand / minimize

[Ref.: #2924]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #2924]

Culture medium growth

[Ref.: #2924]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #2924]

Culture medium composition

expand / minimize

[Ref.: #2924]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #2924]

Culture medium growth

[Ref.: #2924]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #2924]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #2924]	growth	30 °C
[Ref.: #2924]	growth	28 °C
[Ref.: #42154]	growth	30 °C
[Ref.: #46953]	growth	28-37 °C

[Ref.: #2924]	Temperature range	mesophilic
[Ref.: #42154]	Temperature range	mesophilic
[Ref.: #46953]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #46953]

Oxygen tolerance

aerobe

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68374]	arginine ChEBI	-	hydrolysis	* from API ID32E, API 20NE
[Ref.: #68374]	cellobiose ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68374]	D-arabitol ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68374]	D-galacturonic acid ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68369]	D-glucose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68374]	D-glucose ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68374]	D-mannitol ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68369]	D-mannose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	decanoate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	esculin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	gluconate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68374]	L-arabinose ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68374]	L-arabitol ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68374]	L-rhamnose ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68374]	lysine ChEBI	-	degradation	* from API ID32E
[Ref.: #68374]	malonate ChEBI	-	assimilation	* from API ID32E
[Ref.: #68369]	maltose ChEBI	-	assimilation	* from API 20NE
[Ref.: #68374]	maltose ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68374]	myo-inositol ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68369]	N-acetylglucosamine ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #68374]	ornithine ChEBI	-	degradation	* from API ID32E
[Ref.: #68374]	palatinose ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68374]	Potassium 5-ketogluconate	-	builds acid from	* from API ID32E
[Ref.: #68374]	ribitol ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68374]	sorbitol ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68374]	sucrose ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68374]	trehalose ChEBI	-	builds acid from	* from API ID32E
[Ref.: #68374]	tryptophan ChEBI	-	energy source	* from API ID32E, API 20NE
[Ref.: #68374]	urea ChEBI	-	hydrolysis	* from API ID32E, API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite
[Ref.: #68374]		indole ChEBI	* from API ID32E, API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68374]		indole ChEBI	-	* from API ID32E, API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68374]	alpha-galactosidase	-	3.2.1.22	* from API ID32E
[Ref.: #68374]	alpha-glucosidase	-	3.2.1.20	* from API ID32E
[Ref.: #68374]	alpha-maltosidase	-		* from API ID32E
[Ref.: #68374]	arginine dihydrolase	-	3.5.3.6	* from API ID32E, API 20NE
[Ref.: #68374]	beta-galactosidase	-	3.2.1.23	* from API ID32E
[Ref.: #68374]	beta-glucosidase	-	3.2.1.21	* from API ID32E, API 20NE
[Ref.: #68374]	beta-glucuronidase	-	3.2.1.31	* from API ID32E
[Ref.: #2924]	catalase	+	1.11.1.6
[Ref.: #2924]	cytochrome-c oxidase	-	1.9.3.1
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #68374]	L-aspartate arylamidase	+	3.4.11.21	* from API ID32E
[Ref.: #68374]	lipase	-		* from API ID32E
[Ref.: #68374]	lysine decarboxylase	-	4.1.1.18	* from API ID32E
[Ref.: #68374]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API ID32E
[Ref.: #68374]	ornithine decarboxylase	-	4.1.1.17	* from API ID32E
[Ref.: #68374]	urease	-	3.5.1.5	* from API ID32E, API 20NE
	Only first 10 entries are displayed. Click here to see all.

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #2924]	22467	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	+	-	-	+
[Ref.: #2924]	20978	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-
[Ref.: #2924]	20977	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	+	-	not determinedn.d.
[Ref.: #2924]	20976	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	+	-	-	-

API® ID32E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Ornithine decarboxylaseODC	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Urease/urea hydrolysisURE	Acid from L-arabitolLARL	Acid from D-galacturonic acidGAT	Acid from 5-Ketogluconate5KG	LipaseLIP	Phenol red (Acidification)RP	beta-Glucosidasebeta GLU	Acid from D-mannitolMAN	Acid from maltoseMAL	Acid from D-adonitolADO	Acid from palatinosePLE	beta-Glucuronidasebeta GUR	MalonateMNT	Indole productionIND	N-Acetyl-beta-Glucosaminidasebeta NAG	beta-Galactosidasebeta GAL	Acid from D-glucoseGLU	Acid from sucroseSAC	Acid from L-arabinoseLARA	Acid from D-arabitolDARL	alpha-Glucosidasealpha GLU	alpha-Galactosidasealpha GAL	Acid from trehaloseTRE	Acid from L-rhamnoseRHA	Acid from inositolINO	Acid from cellobioseCEL	Acid from sorbitolSOR	alpha-MaltosidasealphaMAL	L-aspartic acid arylamidaseAspA
[Ref.: #46953]	734	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #2924]	Sample type/isolated from	human duodenum

[Ref.: #46953]	Sample type/isolated from	Duodenum
* marker position based on {}

Isolation sources categories

#Host	#Human	-
#Infection	#Patient	-
#Host Body-Site	#Gastrointestinal tract	#Small intestine

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #2924]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Acinetobacter johnsonii a6aw 16S ribosomal RNA gene, partial sequence	AF311990	496	ENA	40214 tax ID	*
[Ref.: #20218]	Acinetobacter johnsonii a6b 16S ribosomal RNA gene, partial sequence	AF311991	496	ENA	40214 tax ID	*
[Ref.: #20218]	Acinetobacter johnsonii a25 16S ribosomal RNA gene, partial sequence	AF311992	493	ENA	40214 tax ID	*
[Ref.: #20218]	Acinetobacter johnsonii a39b 16S ribosomal RNA gene, partial sequence	AF311993	493	ENA	40214 tax ID	*
[Ref.: #20218]	Acinetobacter johnsonii a22 16S ribosomal RNA gene, partial sequence	AF311994	489	ENA	40214 tax ID	*
[Ref.: #20218]	Acinetobacter johnsonii 16S rRNA gene, partial	AJ247202	452	ENA	40214 tax ID	*
[Ref.: #20218]	Acinetobacter johnsonii genomic DNA containing 16S-23S IGS, strain ATCC 17909, clone 01	HE651868	693	ENA	40214 tax ID	*
[Ref.: #20218]	Acinetobacter johnsonii genomic DNA containing 16S-23S IGS, strain ATCC 17909, clone 03	HE651869	707	ENA	40214 tax ID	*
[Ref.: #20218]	Acinetobacter johnsonii genomic DNA containing 16S-23S IGS, strain ATCC 17909, clone 04	HE651870	717	ENA	40214 tax ID	*
[Ref.: #20218]	Acinetobacter johnsonii genomic DNA containing 16S-23S IGS, strain ATCC 17909, clone 05	HE651871	707	ENA	40214 tax ID	*
[Ref.: #20218]	Acinetobacter johnsonii genomic DNA containing 16S-23S IGS, strain ATCC 17909, clone 06	HE651872	731	ENA	40214 tax ID	*
[Ref.: #20218]	Acinetobacter johnsonii genomic DNA containing 16S-23S IGS, strain ATCC 17909, clone 09	HE651873	731	ENA	40214 tax ID	*
[Ref.: #20218]	Acinetobacter johnsonii genomic DNA containing 16S-23S IGS, strain ATCC 17909, clone 11	HE651874	717	ENA	40214 tax ID	*
[Ref.: #20218]	Acinetobacter johnsonii genomic DNA containing 16S-23S IGS, strain ATCC 17909, clone 12	HE651875	707	ENA	40214 tax ID	*
[Ref.: #20218]	Acinetobacter johnsonii genomic DNA containing 16S-23S IGS, strain ATCC 17909, clone 13	HE651876	717	ENA	40214 tax ID	*
[Ref.: #20218]	Acinetobacter johnsonii genomic DNA containing 16S-23S IGS, strain ATCC 17909, clone 14	HE651877	693	ENA	40214 tax ID	*
[Ref.: #20218]	Acinetobacter johnsonii genomic DNA containing 16S-23S IGS, strain ATCC 17909, clone 15	HE651878	693	ENA	40214 tax ID	*
[Ref.: #20218]	Acinetobacter johnsonii genomic DNA containing 16S-23S IGS, strain ATCC 17909, clone 16	HE651879	703	ENA	40214 tax ID	*
[Ref.: #20218]	Acinetobacter johnsonii genomic DNA containing 16S-23S IGS, strain ATCC 17909, clone 18	HE651880	717	ENA	40214 tax ID	*
[Ref.: #20218]	Acinetobacter johnsonii 16S rRNA gene (strain ATCC 17909T)	Z93440	1417	ENA	40214 tax ID	*
[Ref.: #2924]	A.johnsonii 16S rRNA gene (DSM6963)	X81663	1460	ENA	40214 tax ID
[Ref.: #2924]	Acinetobacter johnsonii 16S rRNA gene, strain ATCC 17909	HE651920	1530	ENA	40214 tax ID

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Acinetobacter johnsonii DSM 6963	GCA_000949655	contig	GenBank	40214 tax ID	*
[Ref.: #66792]	Acinetobacter johnsonii CIP 64.6 CIP 64.6	GCA_000368045	scaffold	GenBank	1217663 tax ID	*
[Ref.: #66792]	Acinetobacter johnsonii NCTC10308	GCA_900444855	contig	GenBank	40214 tax ID	*
[Ref.: #66792]	Acinetobacter johnsonii CIP 64.6	1217663.8	wgs	PATRIC	1217663 tax ID	*
[Ref.: #66792]	Acinetobacter johnsonii CIP 64.6	1217663.3	wgs	PATRIC	1217663 tax ID	*
[Ref.: #66792]	Acinetobacter johnsonii strain DSM 6963	40214.29	wgs	PATRIC	40214 tax ID	*

[Ref.: #2924]

GC-content

44.0 mol%

thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

[Ref.: #2924]

Culture collection no.

DSM 6963, ATCC 17909, CIP 64.6, NCTC 10308, CCUG 19095, NCTC 12154

[Ref.: #20218]

Associated Passport(s) in StrainInfo

99496, 51543, 231555, 51555

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Diverse responses to UV-B radiation and repair mechanisms of bacteria isolated from high-altitude aquatic environments.	Fernandez Zenoff V, Sineriz F, Farias ME	Appl Environ Microbiol	10.1128/AEM.01333-06	2006	*
Phylogeny	Acinetobacter kyonggiensis sp. nov., a beta-glucosidase-producing bacterium, isolated from sewage treatment plant.	Lee HJ, Lee SS	J Microbiol	10.1007/s12275-010-0355-8	2011	*
Phylogeny	Acinetobacter kookii sp. nov., isolated from soil.	Choi JY, Ko G, Jheong W, Huys G, Seifert H, Dijkshoorn L, Ko KS	Int J Syst Evol Microbiol	10.1099/ijs.0.047969-0	2013	*

References

#2924

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 6963

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#42154 ; Curators of the CIP;
#46953 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 19095

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68369

Automatically annotated from API 20NE .

#68374

Automatically annotated from API ID32E .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

* These data were automatically processed and therefore are not curated

Change proposal

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