Dactylosporangium matsuzakiense (DSM 43810, ATCC 31570, FERM P-4670)

Colony morphology

Multicellular morphology

Multimedia

• @ref:	11284
  multimedia content:	DSM_43810.jpg
  multimedia.multimedia content:	https://www.dsmz.de/microorganisms/photos/DSM_43810.jpg
  caption:	Medium 554 28°C
  intellectual property rights:	© Leibniz-Institut DSMZ
  manual_annotation:	1

Culture medium

@ref	Name	Growth	Medium link	Composition
11284	N-Z-AMINE-MEDIUM (DSMZ Medium 554)		Medium recipe at MediaDive	Name: N-Z-AMINE-MEDIUM (DSMZ Medium 554) Composition: Starch 20.0 g/l Agar 20.0 g/l Glucose 10.0 g/l N-Z amine 5.0 g/l Yeast extract 5.0 g/l CaCO3 1.0 g/l Distilled water
11284	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)		Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
19958	ISP 2			Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19958	ISP 3			Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19958	ISP 4			Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19958	ISP 5			Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19958	ISP 6			Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19958	ISP 7			Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

Culture temp

Spore formation

Compound production

Halophily

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
19958	22599 ChEBI	arabinose	+/-
68368	29016 ChEBI	arginine	-	hydrolysis	from API 20E
19958	62968 ChEBI	cellulose	+/-
68368	16947 ChEBI	citrate	-	assimilation	from API 20E
19958	28757 ChEBI	fructose	+/-
68368	5291 ChEBI	gelatin	+	hydrolysis	from API 20E
19958	17234 ChEBI	glucose	+/-
68368	25094 ChEBI	lysine	-	degradation	from API 20E
19958	29864 ChEBI	mannitol	+/-
19958	17268 ChEBI	myo-inositol	+/-
68368	18257 ChEBI	ornithine	-	degradation	from API 20E
19958	16634 ChEBI	raffinose	+/-
19958	26546 ChEBI	rhamnose	+/-
19958	17992 ChEBI	sucrose	+/-
68368	27897 ChEBI	tryptophan	-	energy source	from API 20E
68368	16199 ChEBI	urea	-	hydrolysis	from API 20E
19958	18222 ChEBI	xylose	+/-

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68368	15688 ChEBI	acetoin		from API 20E
67770	81430 ChEBI	dactimicin
68368	16136 ChEBI	hydrogen sulfide		from API 20E
68368	35581 ChEBI	indole		from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Indole test
68368	15688 ChEBI	acetoin	+		from API 20E
68368	35581 ChEBI	indole		-	from API 20E

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68368	arginine dihydrolase	-	3.5.3.6	from API 20E
68382	beta-galactosidase	+	3.2.1.23	from API zym
68368	beta-galactosidase	-	3.2.1.23	from API 20E
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68368	gelatinase	+		from API 20E
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68368	lysine decarboxylase	-	4.1.1.18	from API 20E
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
68368	ornithine decarboxylase	-	4.1.1.17	from API 20E
68382	trypsin	+	3.4.21.4	from API zym
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
68368	urease	-	3.5.1.5	from API 20E
68382	valine arylamidase	-		from API zym

API 20E

API zym

Isolation source categories

Isolation

Risk assessment

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
124043	ASM2526472v1 assembly for Dactylosporangium matsuzakiense NRRL B-16293	complete	GCA_025264725	53360.4	8076228864	53360	92.83
124043	ASM3953535v1 assembly for Dactylosporangium matsuzakiense JCM 3311	contig	GCA_039535355			53360	68.2
124043	ASM4242864v1 assembly for Dactylosporangium matsuzakiense JCM 3311	scaffold	GCA_042428645			53360	46.14
124043	ASM2792240v1 assembly for Dactylosporangium matsuzakiense VKM Ac-1321	scaffold	GCA_027922405	53360.5	8104519485	53360

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	D.matsuzakiense 16S rRNA gene	X93193	1469		53360
20218	Dactylosporangium matsuzakiense gene for 16S ribosomal RNA, partial sequence	D86940	1465		53360
124043	Dactylosporangium matsuzakiense strain JCM 3311 16S ribosomal RNA gene, partial sequence.	MT760444	1317		53360

Literature

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Bacterial discrimination by means of a universal array approach mediated by LDR (ligase detection reaction).	Busti E, Bordoni R, Castiglioni B, Monciardini P, Sosio M, Donadio S, Consolandi C, Rossi Bernardi L, Battaglia C, De Bellis G.	BMC Microbiol	10.1186/1471-2180-2-27	2002
	Improving activity of GenB3 and GenB4 in gentamicin dideoxygenation biosynthesis by semi-rational engineering.	Zhai H, Yang L, Ye Q, Kong Z, Pei J, Ji Y, Liu B, Chen X, Tian T, Ni X, Xia H, Zhang S.	Microb Cell Fact	10.1186/s12934-025-02678-0	2025
	Organization and nature of fortimicin A (astromicin) biosynthetic genes studied using a cosmid library of Micromonospora olivasterospora DNA.	Dairi T, Ohta T, Hashimoto E, Hasegawa M.	Mol Gen Genet	10.1007/bf00279641	1992
Enzymology	Pharmacokinetics of dactimicin in rats.	Fraschini F, Scaglione F, Nebuloni R, Falchi M, Balsamo V, Cattaneo G.	Drugs Exp Clin Res		1989
Metabolism	Studies on a new aminoglycoside antibiotic, dactimicin. I. Producing organism and fermentation.	Shomura T, Kojima M, Yoshida J, Ito M, Amano S, Totsugawa K, Niwa T, Inouye S, Ito T, Niida T.	J Antibiot (Tokyo)	10.7164/antibiotics.33.924	1980
Pathogenicity	Characterization of two different types of resistance genes among producers of fortimicin-group antibiotics.	Ohta T, Dairi T, Hasegawa M	J Gen Microbiol	10.1099/00221287-139-3-591	1993
Enzymology	N-formimidoyl fortimicin A synthase, a unique oxidase involved in fortimicin A biosynthesis: purification, characterization and gene cloning.	Dairi T, Yamaguchi K, Hasegawa M	Mol Gen Genet	10.1007/BF00279642	1992
Enzymology	Cloning and analysis of a gene (sms13) encoding sannamycin B-glycyltransferase from Streptomyces sannanensis and its distribution among actinomycetes.	Ohta T, Hashimoto E, Hasegawa M	J Antibiot (Tokyo)	10.7164/antibiotics.45.1167	1992