Dactylosporangium aurantiacum (DSM 43157, ATCC 23491, IFO 12592)

Name: Dactylosporangium aurantiacum (DSM 43157, ATCC 23491, IFO 12592)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7869

Type strain

Strain Designation D-748

Culture col. no. DSM 43157 ATCC 23491 IFO 12592 IMET 9028 JCM 3083 17 more

NCBI tax ID(s) 35754

Special Collections DSMZ JCM KCTC

History <- KCC <- J.E. Thiemann, D-748 KCC A-0083 <-- J. E. Thiemann D-748.

Links

Dactylosporangium aurantiacum D-748 is a bacterium that was isolated from soil.

genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 38041

MTCC 500,ATCC 23491,DSM 43157,IFO 12592,IMET 9028,JCM 3083,CCRC 13312,KCTC 9073,NCIMB 12887,NRRL B-8018,NRRL B-8111,VKM Ac-654,CBS 621.67,AS 4.1066,CECT 3288,NBRC 12592,AS4.1006,BCRC 13312,CGMCC 4.1066,CGMCC 4.1006

@ref 20215

Last LPSN update 2026-02-09 11:19:34

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Micromonosporales

Family Micromonosporaceae

Genus Dactylosporangium

Species Dactylosporangium aurantiacum

Full scientific name Dactylosporangium aurantiacum Thiemann et al. 1967 (Approved Lists 1980)

Morphology

Cell morphology

@ref	Gram stain	Confidence
125439	positive	99.9

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
10825	GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)	Medium recipe at MediaDive	Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
10825	ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84)	Medium recipe at MediaDive	Name: ROLLED OATS MINERAL MEDIUM (DSMZ Medium 84) Composition: Agar 20.0 g/l Rolled oats 20.0 g/l ZnSO4 x 7 H2O 0.001 g/l MnCl2 x 4 H2O 0.001 g/l FeSO4 x 7 H2O 0.001 g/l Distilled water
10825	CZAPEK PEPTONE AGAR (DSMZ Medium 83)	Medium recipe at MediaDive	Name: CZAPEK PEPTONE AGAR (DSMZ Medium 83) Composition: Sucrose 30.0 g/l Agar 20.0 g/l Peptone 5.0 g/l NaNO3 3.0 g/l Yeast extract 2.0 g/l K2HPO4 1.0 g/l MgSO4 x 7 H2O 0.5 g/l KCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
10825	positive	growth	28
67770	positive	growth	28

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125439	obligate aerobe	99.6

Observation

67770

Observationquinones: MK-9(H₈), MK-9(H₆)

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type
10825	soil
67770	Soil

Taxonmaps

69479

Global distribution of 16S sequence U58528 (>99% sequence identity) for Dactylosporangium from Microbeatlas

Aquatic Samples	58
Soil Samples	1581
Animal Samples	75
Plant Samples	21
Total Samples	1735

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
10825	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
124043	ASM2526470v1 assembly for Dactylosporangium aurantiacum NRRL B-8018	complete	GCA_025264705	35754.8	8049210031	35754	92.4
124043	ASM2975980v1 assembly for Dactylosporangium aurantiacum ATCC 23491	scaffold	GCA_029759805	35754.10	8118249771	35754	36.31
67770	ASM71671v1 assembly for Dactylosporangium aurantiacum NRRL B-8018	contig	GCA_000716715	35754.4	2772190741	35754	26.26
67770	ASM71605v1 assembly for Dactylosporangium aurantiacum NRRL B-8111	scaffold	GCA_000716055	35754.3	2806310743	35754

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Dactylosporangium aurantiacum 16S ribosomal RNA gene sequence	U58528	1410	35754
20218	D.aurantiacum gene for 16S rRNA (DSM 43157)	X72779	1358	35754
20218	D.aurantiacum 16S rRNA gene	X93191	1476	35754
20218	Dactylosporangium aurantiacum gene for 16S ribosomal RNA, partial sequence	D85480	1472	35754
124043	Dactylosporangium aurantiacum strain ATCC 23491 16S ribosomal RNA gene, partial sequence.	OQ352622	1355	35754

GC content

@ref	GC-content (mol%)	Method
67770	73	thermal denaturation, midpoint method (Tm)
67770	73.2	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Dactylosporangium aurantiacum NRRL B-8018
NCBI: GCA_000716715

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	84.60	no
125439	motility	BacteriaNetⓘ	no	84.90	no
125439	gram_stain	BacteriaNetⓘ	positive	99.90	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	99.60	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Dactylosporangium aurantiacum NRRL B-8111
NCBI: GCA_000716055.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	85.64	no
125438	anaerobic	anaerobicⓘ	no	95.88	no
125438	aerobic	aerobicⓘ	yes	86.52	no
125438	spore-forming	spore-formingⓘ	yes	89.76	no
125438	thermophilic	thermophileⓘ	no	93.99	no
125438	flagellated	motile2+ⓘ	no	63.33	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Dactylides A-C, three new bioactive 22-membered macrolides produced by Dactylosporangium aurantiacum.	Kumar P, Nalli Y, Singh S, Wakchaure PD, Gor R, Ghadge VA, Kim E, Ramalingam S, Azger Dusthackeer VN, Yoon YJ, Ganguly B, Shinde PB.	J Antibiot (Tokyo)	10.1038/s41429-023-00632-z	2023
Phylogeny	Real-time PCR for the detection and quantification of geodermatophilaceae from stone samples and identification of new members of the genus blastococcus.	Salazar O, Valverde A, Genilloud O.	Appl Environ Microbiol	10.1128/aem.72.1.346-352.2006	2006
Enzymology	Analysis of actinomycete communities by specific amplification of genes encoding 16S rRNA and gel-electrophoretic separation in denaturing gradients.	Heuer H, Krsek M, Baker P, Smalla K, Wellington EM.	Appl Environ Microbiol	10.1128/aem.63.8.3233-3241.1997	1997
Metabolism	Reassociation of deoxyribonucleic acids from Actinoplanes and other actinomycetes.	Farina G, Bradley SG.	J Bacteriol	10.1128/jb.102.1.30-35.1970	1970
Metabolism	Denitrification by actinomycetes and purification of dissimilatory nitrite reductase and azurin from Streptomyces thioluteus.	Shoun H, Kano M, Baba I, Takaya N, Matsuo M.	J Bacteriol	10.1128/jb.180.17.4413-4415.1998	1998
	Biosynthetic Diversification of Fidaxomicin Aglycones by Heterologous Expression and Promoter Refactoring.	Wang L, Zhu M, Zhang Q, Zhai S, Zhu Y, Zhang H, Zhang C.	J Nat Prod	10.1021/acs.jnatprod.3c00001	2023
Genetics	Stepwise increase of fidaxomicin in an engineered heterologous host Streptomyces albus through multi-level metabolic engineering.	Xie H, Su YT, Bu QT, Li YP, Zhao QW, Du YL, Li YQ.	Synth Syst Biotechnol	10.1016/j.synbio.2024.06.004	2024
	Assessing the effect of temperature on Rhodococcus metabolite production.	Macias-Contreras MI, Millan-Aguinaga N, Parra J, Duncan KR.	Microbiology (Reading)	10.1099/mic.0.001598	2025
Transcriptome	Transcriptional regulation of the fidaxomicin gene cluster and cellular development in Actinoplanes deccanensis YP-1 by the pleiotropic regulator MtrA.	Xie H, Ruan J-Y, Bu Q-T, Li Y-P, Su Y-T, Zhao Q-W, Du Y-L, Li Y-Q.	Microbiol Spectr	10.1128/spectrum.02702-23	2023
	Improving fidaxomicin production through ARTP mutagenesis and fermentation optimization in Actinoplanes deccanensis.	Ruan JY, Xie H, Zheng YL, Zhao QW, Chen XA, Lyu ZY, Li YQ.	Synth Syst Biotechnol	10.1016/j.synbio.2025.06.002	2025
	Tiacumicin Congeners with Improved Antibacterial Activity from a Halogenase-Inactivated Mutant.	Zhang H, Tian X, Pu X, Zhang Q, Zhang W, Zhang C.	J Nat Prod	10.1021/acs.jnatprod.7b00990	2018
Metabolism	Functional characterization of salt-tolerant microbial esterase WDEst17 and its use in the generation of optically pure ethyl (R)-3-hydroxybutyrate.	Wang Y, Xu Y, Zhang Y, Sun A, Hu Y.	Chirality	10.1002/chir.22847	2018
	The Co-identity of Lipiarmycin A3 and Tiacumicin B.	Bedeschi A, Fonte P, Fronza G, Fuganti C, Serra S.	Nat Prod Commun	10.1177/1934578x1400900227	2016
Metabolism	Functional Characterization of a Novel Dactylosporangium Esterase and Its Utilization in the Asymmetric Synthesis of (R)-Methyl Mandelate.	Deng D, Zhang Y, Sun A, Hu Y.	Appl Biochem Biotechnol	10.1007/s12010-016-2095-7	2016
	Comparison of fidaxomicin, thuricin CD, vancomycin and nisin highlights the narrow spectrum nature of thuricin CD.	Walsh L, Lavelle A, O'Connor PM, Hill C, Ross RP.	Gut Microbes	10.1080/19490976.2024.2342583	2024
	The ssgB gene is required for the early stages of sporangium formation in Actinoplanes missouriensis.	Akutsu T, Tezuka T, Maruko M, Hirata A, Ohnishi Y.	J Bacteriol	10.1128/jb.00428-23	2024
Enzymology	Role of fidaxomicin for the treatment of Clostridium difficile infection.	Juang P, Hardesty JS.	J Pharm Pract	10.1177/0897190013499526	2013
	Characterization of a sugar-O-methyltransferase TiaS5 affords new Tiacumicin analogues with improved antibacterial properties and reveals substrate promiscuity.	Niu S, Hu T, Li S, Xiao Y, Ma L, Zhang G, Zhang H, Yang X, Ju J, Zhang C.	Chembiochem	10.1002/cbic.201100129	2011
Enzymology	Fidaxomicin for the treatment of Clostridioides difficile in children.	Skinner AM, Scardina T, Kociolek LK.	Future Microbiol	10.2217/fmb-2020-0104	2020
	Drug evaluation: OPT-80, a narrow-spectrum macrocyclic antibiotic.	Johnson AP.	Curr Opin Investig Drugs		2007
	Final Demonstration of the Co-Identity of Lipiarmycin A3 and Tiacumicin B (Fidaxomicin) through Single Crystal X-ray Analysis.	Serra S, Malpezzi L, Bedeschi A, Fuganti C, Fonte P.	Antibiotics (Basel)	10.3390/antibiotics6010007	2017
Pathogenicity	Biofilm Formation of Clostridioides difficile, Toxin Production and Alternatives to Conventional Antibiotics in the Treatment of CDI.	Dicks LMT.	Microorganisms	10.3390/microorganisms11092161	2023
	Identification of BgP, a Cutinase-Like Polyesterase From a Deep-Sea Sponge-Derived Actinobacterium.	Carr CM, de Oliveira BFR, Jackson SA, Laport MS, Clarke DJ, Dobson ADW.	Front Microbiol	10.3389/fmicb.2022.888343	2022
	Production of brominated tiacumicin derivatives	Hochlowski JE, Jackson M, Rasmussen RR, Buko AM, Clement JJ, Whittern DN, McAlpine JB.	J Antibiot (Tokyo)		1997
Metabolism	Characterization of Zoospore Type IV Pili in Actinoplanes missouriensis.	Kimura T, Tezuka T, Nakane D, Nishizaka T, Aizawa SI, Ohnishi Y.	J Bacteriol	10.1128/jb.00746-18	2019
Enzymology	Tiacumicins, a novel complex of 18-membered macrolides. II. Isolation and structure determination.	Hochlowski JE, Swanson SJ, Ranfranz LM, Whittern DN, Buko AM, McAlpine JB.	J Antibiot (Tokyo)	10.7164/antibiotics.40.575	1987
	Antibiotic Treatments for Clostridium difficile Infection Are Associated with Distinct Bacterial and Fungal Community Structures.	Lamendella R, Wright JR, Hackman J, McLimans C, Toole DR, Bernard Rubio W, Drucker R, Wong HT, Sabey K, Hegarty JP, Stewart DB.	mSphere	10.1128/msphere.00572-17	2018
Phylogeny	A new antibiotic SF-2185 produced by Dactylosporangium. I. Taxonomy, fermentation and biological properties.	Matsumoto K, Shomura T, Shimura M, Yoshida J, Ito M, Watanabe T, Ito T.	J Antibiot (Tokyo)	10.7164/antibiotics.38.1487	1985
Enzymology	Tiacumicins, a novel complex of 18-membered macrolide antibiotics. I. Taxonomy, fermentation and antibacterial activity.	Theriault RJ, Karwowski JP, Jackson M, Girolami RL, Sunga GN, Vojtko CM, Coen LJ.	J Antibiot (Tokyo)	10.7164/antibiotics.40.567	1987
Pathogenicity	Natural products as inspiration for the development of bacterial antibiofilm agents.	Melander RJ, Basak AK, Melander C.	Nat Prod Rep	10.1039/d0np00022a	2020
Genetics	Genome-Based Taxonomic Classification of the Phylum Actinobacteria.	Nouioui I, Carro L, Garcia-Lopez M, Meier-Kolthoff JP, Woyke T, Kyrpides NC, Pukall R, Klenk HP, Goodfellow M, Goker M.	Front Microbiol	10.3389/fmicb.2018.02007	2018
Phylogeny	Dactylosporangium luridum sp. nov., Dactylosporangium luteum sp. nov. and Dactylosporangium salmoneum sp. nov., nom. rev., isolated from soil.	Kim BY, Stach JEM, Weon HY, Kwon SW, Goodfellow M.	Int J Syst Evol Microbiol	10.1099/ijs.0.016451-0	2010
Phylogeny	Dactylosporangium cerinum sp. nov., a novel actinobacterium isolated from the rhizosphere of Pinus koraiensis Sieb. et Zucc.	Liu C, Guan X, Jin P, Li J, Li Y, Li L, Zhou Y, Shen Y, Wang X, Xiang W	Antonie Van Leeuwenhoek	10.1007/s10482-015-0478-3	2015
Phylogeny	Dactylosporangium maewongense sp. nov., isolated from soil.	Chiaraphongphon S, Suriyachadkun C, Tamura T, Thawai C	Int J Syst Evol Microbiol	10.1099/ijs.0.016378-0	2009

References

#10825	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 43157
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Rate our new design

Content

About us

Dactylosporangium aurantiacum (DSM 43157, ATCC 23491, IFO 12592)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Observation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_025264705

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

Help

Social Media