Name: Actinoplanes cyaneus DSM 46137
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 7816

Type strain:

Species: Actinoplanes cyaneus

Culture col. no.: DSM 46137, IFO 14990, IMET 9243, INA 1569, JCM 9082, NBRC 14990, NCIMB 12736, VKM Ac-1095, BCC 49182, BCRC 16342, KCTC 9590, TBRC 174

Strain history: VKM Ac-1095 <-- INA 1569.

NCBI tax ID(s): 52696 (species)

SI-ID 297058

DSM 46137, IMET 9243, JCM 9082, IFO 14990, NCIMB 12736, KCTC 9590, NBRC 14990, BCRC 16342, CCRC 16342

Other strains from Actinoplanes cyaneus

A. cyaneus STH00303(ZIMET), HKI 0303

Section

Actinoplanes cyaneus DSM 46137 is a spore-forming, mesophilic, Gram-positive bacterium that was isolated from soil.

Gram-positive
spore-forming
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micromonosporales*
	Family	*Micromonosporaceae*
	Genus	*Actinoplanes*
	Species	**Actinoplanes cyaneus**
	Full Scientific Name (LPSN)	Actinoplanes cyaneus Terekhova et al. 1987

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #20192]	Colony color	Pastel orange (2003)
[Ref.: #20192]	Incubation period	10-14 days
[Ref.: #20192]	Cultivation medium used	ISP 2

[Ref.: #20192]	Colony color	Ochre yellow (1024)
[Ref.: #20192]	Incubation period	10-14 days
[Ref.: #20192]	Cultivation medium used	ISP 3

[Ref.: #20192]	Colony color	Pastel yellow (1034)
[Ref.: #20192]	Incubation period	10-14 days
[Ref.: #20192]	Cultivation medium used	ISP 4

[Ref.: #20192]	Colony color	Pastel yellow (1034)
[Ref.: #20192]	Incubation period	10-14 days
[Ref.: #20192]	Cultivation medium used	ISP 5

[Ref.: #20192]	Colony color	Pastel yellow (1034)
[Ref.: #20192]	Incubation period	10-14 days
[Ref.: #20192]	Cultivation medium used	ISP 6

[Ref.: #20192]	Colony color	Pastel yellow (1034)
[Ref.: #20192]	Incubation period	10-14 days
[Ref.: #20192]	Cultivation medium used	ISP 7

[Ref.: #20192]	Multicellular complex forming ability	no
[Ref.: #20192]	Medium Name (multicellularity)	ISP 2

[Ref.: #20192]	Multicellular complex forming ability	no
[Ref.: #20192]	Medium Name (multicellularity)	ISP 3

[Ref.: #20192]	Multicellular complex forming ability	no
[Ref.: #20192]	Medium Name (multicellularity)	ISP 4

[Ref.: #20192]	Multicellular complex forming ability	no
[Ref.: #20192]	Medium Name (multicellularity)	ISP 5

[Ref.: #20192]	Multicellular complex forming ability	no
[Ref.: #20192]	Medium Name (multicellularity)	ISP 6

[Ref.: #20192]	Multicellular complex forming ability	no
[Ref.: #20192]	Medium Name (multicellularity)	ISP 7

Information on culture and growth conditions Culture and growth conditions

[Ref.: #20192]

Culture medium

ISP 2

[Ref.: #20192]

Culture medium growth

[Ref.: #20192]

Culture medium composition

expand / minimize

[Ref.: #20192]

Culture medium

ISP 3

[Ref.: #20192]

Culture medium growth

[Ref.: #20192]

Culture medium composition

expand / minimize

[Ref.: #20192]

Culture medium

ISP 4

[Ref.: #20192]

Culture medium growth

[Ref.: #20192]

Culture medium composition

expand / minimize

[Ref.: #20192]

Culture medium

ISP 5

[Ref.: #20192]

Culture medium growth

[Ref.: #20192]

Culture medium composition

expand / minimize

[Ref.: #20192]

Culture medium

ISP 6

[Ref.: #20192]

Culture medium growth

[Ref.: #20192]

Culture medium composition

expand / minimize

[Ref.: #20192]

Culture medium

ISP 7

[Ref.: #20192]

Culture medium growth

[Ref.: #20192]

Culture medium composition

expand / minimize

[Ref.: #12460]

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

[Ref.: #12460]

Culture medium growth

[Ref.: #12460]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #12460]

Culture medium

GPHF-MEDIUM (DSMZ Medium 553)

[Ref.: #12460]

Culture medium growth

[Ref.: #12460]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #12460]	growth	28 °C
[Ref.: #20192]	optimum	28 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #12460]	Temperature range	mesophilic
[Ref.: #20192]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

[Ref.: #12460]

Name of produced compound

actinorhodin

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #20192]		NaCl		maximum	2.5	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68368]	arginine ChEBI	+	hydrolysis	* from API 20E
[Ref.: #68368]	citrate ChEBI	+	assimilation	* from API 20E
[Ref.: #68368]	gelatin ChEBI	+	hydrolysis	* from API 20E
[Ref.: #68368]	lysine ChEBI	+	degradation	* from API 20E
[Ref.: #68368]	ornithine ChEBI	+	degradation	* from API 20E
[Ref.: #68368]	tryptophan ChEBI	-	energy source	* from API 20E
[Ref.: #68368]	urea ChEBI	+	hydrolysis	* from API 20E

	Metabolite	Production
[Ref.: #68368]	acetoin ChEBI	yes	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68368]	indole ChEBI	no	* from API 20E

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #68368]		acetoin ChEBI	+		* from API 20E
[Ref.: #68368]		indole ChEBI		-	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68368]	arginine dihydrolase	+	3.5.3.6	* from API 20E
[Ref.: #68368]	beta-galactosidase	-	3.2.1.23	* from API 20E
[Ref.: #68368]	gelatinase	+		* from API 20E
[Ref.: #68368]	lysine decarboxylase	+	4.1.1.18	* from API 20E
[Ref.: #68368]	ornithine decarboxylase	+	4.1.1.17	* from API 20E
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #68368]	urease	+	3.5.1.5	* from API 20E

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #20192]	636	-	+	+	+	+	-	+	-	-	+	+	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #12460]	Sample type/isolated from	soil

[Ref.: #67770]	Sample type/isolated from	Soil
[Ref.: #67770]	Geographic location (country and/or sea, region)	Siberia
[Ref.: #67770]	Country	Russia
[Ref.: #67770]	Country ISO 3 Code	RUS
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence X93186 (>99% sequence identity) for Actinoplanes from Microbeatlas

Aquatic Samples	16
Soil Samples	190
Animal Samples	73
Plant Samples	20
Total Samples	299

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #12460]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #20192]	Biosafety level	1	German classification

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	A.cyaneus 16S rRNA gene	X93186	1438	ENA	52696 tax ID	*
[Ref.: #20218]	Actinoplanes cyaneus gene for 16S rRNA, partial sequence	AB036997	1469	ENA	52696 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Actinoplanes cyaneus NBRC 14990	GCA_016862095	contig	GenBank	52696 tax ID	*
[Ref.: #66792]	Actinoplanes cyaneus strain NBRC 14990	52696.3	wgs	PATRIC	52696 tax ID	*
[Ref.: #66792]	Actinoplanes cyaneus DSM 46137	2724679919	draft	JGI IMG/M	52696 tax ID	*
[Ref.: #66792]	Actinoplanes cyaneus DSM 46137	GCA_025960925	scaffold	GenBank	52696 tax ID	*
[Ref.: #66792]	Actinoplanes cyaneus DSM 46137	GCA_900182945	scaffold	GenBank	52696 tax ID	*

[Ref.: #67770]

GC-content

71 mol%

genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Actinoplanes cyaneus strain NBRC 14990 PATRIC: 52696.3
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Actinoplanes cyaneus strain NBRC 14990 PATRIC: 52696.3
[Ref.: #69480]	gram-positive	yes	88.099	no
[Ref.: #69480]	anaerobic	no	99.037	no
[Ref.: #69480]	halophile	no	95.604	no
[Ref.: #69480]	spore-forming	yes	94.002	no
[Ref.: #69480]	glucose-util	yes	89.932	no
[Ref.: #69480]	thermophile	no	99.368	yes
[Ref.: #69480]	motile	yes	65.651	no
[Ref.: #69480]	aerobic	yes	93.325	no
[Ref.: #69480]	flagellated	no	89.855	no
[Ref.: #69480]	glucose-ferment	no	89.262	no

Availability in culture collections External links

[Ref.: #12460]

Culture collection no.

DSM 46137, IFO 14990, IMET 9243, INA 1569, JCM 9082, NBRC 14990, NCIMB 12736, VKM Ac-1095, BCC 49182, BCRC 16342, KCTC 9590, TBRC 174

[Ref.: #77258]

SI-ID 297058

References

#12460

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 46137

#20192

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68368

Automatically annotated from API 20E .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#77258

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID297058.1 )

* These data were automatically processed and therefore are not curated

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Actinoplanes cyaneus strain NBRC 14990

Actinoplanes cyaneus strain NBRC 14990

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