Name: Arthrobacter tecti R-5369
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 7608

Type strain:

Species: Arthrobacter tecti

Strain Designation: R-5369

Culture col. no.: DSM 16407, LMG 22282, JCM 21772, IAM 15323

Strain history: IAM 15323 <-- LMG 22282 <-- J. Heyrman R-5369.

NCBI tax ID(s): 271433 (species)

SI-ID 19294

LMG 22282, DSM 16407, JCM 21772, IAM 15323

Special Collections

Section

Arthrobacter tecti R-5369 is an aerobe, Gram-positive, coccus-shaped bacterium that was isolated from Biofilm covering the Servilia tomb, Roman necropolis of Carmona.

coccus-shaped
Gram-positive
aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Micrococcaceae*
	Genus	*Arthrobacter*
	Species	**Arthrobacter tecti**
	Full Scientific Name (LPSN)	Arthrobacter tecti Heyrman et al. 2005

Information on morphological and physiological properties Morphology

[Ref.: #31342]	Gram stain	positive

[Ref.: #31342]	Cell width	0.9 µm

[Ref.: #31342]	Cell shape	coccus-shaped

[Ref.: #31342]	Motility	no

[Ref.: #18403]	Colony color	Rapeseed yellow (1021)
[Ref.: #18403]	Incubation period	10-14 days
[Ref.: #18403]	Cultivation medium used	ISP 2

[Ref.: #18403]	Colony color	Lemon yellow (1012)
[Ref.: #18403]	Incubation period	10-14 days
[Ref.: #18403]	Cultivation medium used	ISP 3

[Ref.: #18403]	Colony color	Lemon yellow (1012)
[Ref.: #18403]	Incubation period	10-14 days
[Ref.: #18403]	Cultivation medium used	ISP 4

[Ref.: #18403]	Colony color	Lemon yellow (1012)
[Ref.: #18403]	Incubation period	10-14 days
[Ref.: #18403]	Cultivation medium used	ISP 5

[Ref.: #18403]	Colony color	Lemon yellow (1012)
[Ref.: #18403]	Incubation period	10-14 days
[Ref.: #18403]	Cultivation medium used	ISP 6

[Ref.: #18403]	Colony color	Lemon yellow (1012)
[Ref.: #18403]	Incubation period	10-14 days
[Ref.: #18403]	Cultivation medium used	ISP 7

[Ref.: #31342]

Pigment production

[Ref.: #18403]	Multicellular complex forming ability	no
[Ref.: #18403]	Medium Name (multicellularity)	ISP 2

[Ref.: #18403]	Multicellular complex forming ability	no
[Ref.: #18403]	Medium Name (multicellularity)	ISP 3

[Ref.: #18403]	Multicellular complex forming ability	no
[Ref.: #18403]	Medium Name (multicellularity)	ISP 4

[Ref.: #18403]	Multicellular complex forming ability	no
[Ref.: #18403]	Medium Name (multicellularity)	ISP 5

[Ref.: #18403]	Multicellular complex forming ability	no
[Ref.: #18403]	Medium Name (multicellularity)	ISP 6

[Ref.: #18403]	Multicellular complex forming ability	no
[Ref.: #18403]	Medium Name (multicellularity)	ISP 7

Information on culture and growth conditions Culture and growth conditions

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Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

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Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

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Culture medium

ISP 2

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Culture medium growth

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Culture medium composition

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Culture medium

ISP 7

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Culture medium growth

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Culture medium composition

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Culture medium

ISP 6

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Culture medium growth

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Culture medium composition

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Culture medium

ISP 5

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Culture medium growth

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Culture medium composition

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Culture medium

ISP 4

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Culture medium growth

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Culture medium composition

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Culture medium

ISP 3

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Culture medium growth

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Culture medium composition

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		Temperature
[Ref.: #6436]	growth	28 °C
[Ref.: #18403]	optimum	28 °C
[Ref.: #31342]	optimum	26 °C
[Ref.: #67770]	growth	28 °C

	pH	Kind of pH		pH
[Ref.: #31342]			optimum	8
[Ref.: #31342]			growth	06-09

Information on physiology and metabolism Physiology and metabolism

[Ref.: #31342]

Oxygen tolerance

aerobe

[Ref.: #31342]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #31342]		NaCl		growth	05-15	%
[Ref.: #31342]		NaCl		optimum	10	%

[Ref.: #6436]	Murein short key	A11.28
[Ref.: #6436]	Murein types	A3alpha L-Lys-L-Thr-L-Ala₃

[Ref.: #31342]	Observation	aggregates in clumps
[Ref.: #67770]	Observation	quinones: MK-9(H₂), MK-10(H₂)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #18403]	arabinose ChEBI	+
[Ref.: #18403]	cellulose ChEBI	+
[Ref.: #68379]	esculin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #18403]	fructose ChEBI	+/-
[Ref.: #31342]	gelatin ChEBI	+	carbon source
[Ref.: #68379]	gelatin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #18403]	glucose ChEBI	+
[Ref.: #18403]	mannitol ChEBI	+/-
[Ref.: #18403]	myo-inositol ChEBI	-
[Ref.: #18403]	raffinose ChEBI	+
[Ref.: #18403]	rhamnose ChEBI	+
[Ref.: #18403]	sucrose ChEBI	+
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	urea ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #18403]	xylose ChEBI	-
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #31342]	acid phosphatase	+	3.1.3.2
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #31342]	alkaline phosphatase	+	3.1.3.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym, API Coryne
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym, API Coryne
[Ref.: #68382]	alpha-mannosidase	+	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym, API Coryne
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym, API Coryne
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym, API Coryne
[Ref.: #31342]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #31342]	gelatinase	+
[Ref.: #68379]	gelatinase	-		* from API Coryne
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym, API Coryne
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68379]	urease	+	3.5.1.5	* from API Coryne
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #18403]	88	not determinedn.d.	-	-	+	-	-	-	+	-	+	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	-	not determinedn.d.	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #18403]	113	not determinedn.d.	+	+	+	-	+	+	-	-	-	+	+	-	-	-	-	-	+	+	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #6436]	Sample type/isolated from	Biofilm covering the Servilia tomb, Roman necropolis of Carmona
[Ref.: #6436]	Geographic location (country and/or sea, region)	Carmona
[Ref.: #6436]	Country	Spain
[Ref.: #6436]	Country ISO 3 Code	ESP
[Ref.: #6436]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Biofilm overgrowing the ceiling of the main room of the Servilia tomb
[Ref.: #67770]	Geographic location (country and/or sea, region)	Roman necropolis of Carmona
[Ref.: #67770]	Country	Spain
[Ref.: #67770]	Country ISO 3 Code	ESP
[Ref.: #67770]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Environmental	#Biofilm	-
#Environmental	#Terrestrial	-

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #18403]	Biosafety level	2
[Ref.: #6436]	Biosafety level	1	Risk group (German classification) According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #31342]	Arthrobacter tecti partial 16S rRNA gene, strain LMG 22285	AJ639828	1480	GenBank ENA	271433 tax ID
[Ref.: #6436]	Arthrobacter tecti partial 16S rRNA gene, type strain LMG 22282T	AJ639829	1476	GenBank ENA	271433 tax ID

[Ref.: #6436]	GC-content	63.7 mol%
[Ref.: #67770]	GC-content	63.7 mol%	high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #6436]

Culture collection no.

DSM 16407, LMG 22282, JCM 21772, IAM 15323

[Ref.: #77052]

SI-ID 19294

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Six novel Arthrobacter species isolated from deteriorated mural paintings.	Heyrman J, Verbeeren J, Schumann P, Swings J, De Vos P	Int J Syst Evol Microbiol	10.1099/ijs.0.63358-0	2005	*

References

#6436

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 16407

#18403

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#31342

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27656 (see below)

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#77052

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID19294.1 )

#27656

IJSEM 1457 2005 ( DOI 10.1099/ijs.0.63358-0 , PubMed 16014466 )

* These data were automatically processed and therefore are not curated

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