Mycetocola lacteus (DSM 15177, IFO 16278, NBRC 16278)

Name: Mycetocola lacteus (DSM 15177, IFO 16278, NBRC 16278)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7468

Type strain

Strain Designation CM-10

Culture col. no. DSM 15177 IFO 16278 NBRC 16278 NRRL B-24121 CM-10 5 more

NCBI tax ID(s) 76637

Special Collections DSMZ JCM Wink compendium

History <- NBRC <- T. Tsukamoto; CM-10 IFO 16278 <-- T. Tsukamoto CM-10.

Links

Mycetocola lacteus CM-10 is a bacterium that was isolated from cultivated mushroom Pleurotus ostreatus.

genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 62367

IFO 16278,DSM 15177,NRRL B-24121,JCM 11654,NBRC 16278,LMG 22187,IAM 15091

@ref 20215

Last LPSN update 2026-02-09 11:19:29

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Micrococcales

Family Microbacteriaceae

Genus Mycetocola

Species Mycetocola lacteus

Full scientific name Mycetocola lacteus Tsukamoto et al. 2001

Morphology

Cell morphology

@ref	Motility	Confidence	Gram stain
125438		91.5
125438		91.992	positive

Colony morphology

@ref	Colony color	Incubation period	Medium used
18519	Beige (1001)	10-14 days	ISP 2
18519	Beige (1001)	10-14 days	ISP 3
18519		10-14 days	ISP 4
18519		10-14 days	ISP 5
18519		10-14 days	ISP 6
18519		10-14 days	ISP 7

Multicellular morphology

@ref	Forms multicellular complex	Medium name
18519		ISP 2
18519		ISP 3
18519		ISP 4
18519		ISP 5
18519		ISP 6
18519		ISP 7

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
5742	TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)	Medium recipe at MediaDive	Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
18519	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18519	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18519	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18519	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18519	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18519	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

Culture temp

@ref	Growth	Type	Temperature (°C)
5742	positive	growth	28
18519	positive	optimum	28
67770	positive	growth	30

Physiology and metabolism

Observation

67770

Observationquinones: MK-10, MK-9, MK-11

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
18519	22599 ChEBI	arabinose	-
18519	62968 ChEBI	cellulose	-
68379	4853 ChEBI	esculin	+	hydrolysis	from API Coryne
18519	28757 ChEBI	fructose	+/-
68379	5291 ChEBI	gelatin	-	hydrolysis	from API Coryne
18519	17234 ChEBI	glucose	-
18519	29864 ChEBI	mannitol	+/-
18519	17268 ChEBI	myo-inositol	-
18519	16634 ChEBI	raffinose	-
18519	26546 ChEBI	rhamnose	-
18519	17992 ChEBI	sucrose	-
68379	17992 ChEBI	sucrose	-	fermentation	from API Coryne
18519	18222 ChEBI	xylose	+

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68379	alkaline phosphatase	-	3.1.3.1	from API Coryne
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68379	alpha-glucosidase	+	3.2.1.20	from API Coryne
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	+	3.2.1.23	from API zym
68379	beta-galactosidase	+	3.2.1.23	from API Coryne
68382	beta-glucosidase	+	3.2.1.21	from API zym
68379	beta-glucosidase	+	3.2.1.21	from API Coryne
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68379	beta-glucuronidase	-	3.2.1.31	from API Coryne
68382	cystine arylamidase	+	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68379	gelatinase	-		from API Coryne
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68379	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API Coryne
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
68379	pyrazinamidase	+	3.5.1.B15	from API Coryne
68379	pyrrolidonyl arylamidase	+	3.4.19.3	from API Coryne
68382	trypsin	-	3.4.21.4	from API zym
68382	valine arylamidase	+		from API zym

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	suberin monomers biosynthesis	100	2 of 2
66794	acetate fermentation	100	4 of 4
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	vitamin K metabolism	100	5 of 5
66794	palmitate biosynthesis	100	22 of 22
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	coenzyme A metabolism	100	4 of 4
66794	formaldehyde oxidation	100	3 of 3
66794	adipate degradation	100	2 of 2
66794	ppGpp biosynthesis	100	4 of 4
66794	methylglyoxal degradation	100	5 of 5
66794	folate polyglutamylation	100	1 of 1
66794	valine metabolism	100	9 of 9
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	pentose phosphate pathway	90.91	10 of 11
66794	threonine metabolism	90	9 of 10
66794	Entner Doudoroff pathway	90	9 of 10
66794	chorismate metabolism	88.89	8 of 9
66794	ketogluconate metabolism	87.5	7 of 8
66794	peptidoglycan biosynthesis	80	12 of 15
66794	flavin biosynthesis	80	12 of 15
66794	glycogen metabolism	80	4 of 5
66794	pyrimidine metabolism	80	36 of 45
66794	cellulose degradation	80	4 of 5
66794	glutamate and glutamine metabolism	78.57	22 of 28
66794	photosynthesis	78.57	11 of 14
66794	serine metabolism	77.78	7 of 9
66794	gluconeogenesis	75	6 of 8
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	isoleucine metabolism	75	6 of 8
66794	sulfopterin metabolism	75	3 of 4
66794	dTDPLrhamnose biosynthesis	75	6 of 8
66794	proline metabolism	72.73	8 of 11
66794	metabolism of disaccharids	72.73	8 of 11
66794	alanine metabolism	72.41	21 of 29
66794	purine metabolism	72.34	68 of 94
66794	NAD metabolism	72.22	13 of 18
66794	propanol degradation	71.43	5 of 7
66794	cardiolipin biosynthesis	71.43	5 of 7
66794	glycolysis	70.59	12 of 17
66794	phenylalanine metabolism	69.23	9 of 13
66794	vitamin B1 metabolism	69.23	9 of 13
66794	aspartate and asparagine metabolism	66.67	6 of 9
66794	enterobactin biosynthesis	66.67	2 of 3
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	CO2 fixation in Crenarchaeota	66.67	6 of 9
66794	cyanate degradation	66.67	2 of 3
66794	d-mannose degradation	66.67	6 of 9
66794	acetoin degradation	66.67	2 of 3
66794	histidine metabolism	65.52	19 of 29
66794	heme metabolism	64.29	9 of 14
66794	citric acid cycle	64.29	9 of 14
66794	d-xylose degradation	63.64	7 of 11
66794	degradation of sugar alcohols	62.5	10 of 16
66794	C4 and CAM-carbon fixation	62.5	5 of 8
66794	oxidative phosphorylation	61.54	56 of 91
66794	starch degradation	60	6 of 10
66794	degradation of sugar acids	60	15 of 25
66794	metabolism of amino sugars and derivatives	60	3 of 5
66794	lipoate biosynthesis	60	3 of 5
66794	lysine metabolism	59.52	25 of 42
66794	arginine metabolism	58.33	14 of 24
66794	methionine metabolism	57.69	15 of 26
66794	isoprenoid biosynthesis	57.69	15 of 26
66794	tetrahydrofolate metabolism	57.14	8 of 14
66794	cysteine metabolism	55.56	10 of 18
66794	non-pathway related	55.26	21 of 38
66794	tryptophan metabolism	55.26	21 of 38
66794	lipid metabolism	54.84	17 of 31
66794	leucine metabolism	53.85	7 of 13
66794	urea cycle	53.85	7 of 13
66794	degradation of pentoses	50	14 of 28
66794	propionate fermentation	50	5 of 10
66794	glycine metabolism	50	5 of 10
66794	ethanol fermentation	50	1 of 2
66794	quinate degradation	50	1 of 2
66794	degradation of aromatic, nitrogen containing compounds	50	6 of 12
66794	glycolate and glyoxylate degradation	50	3 of 6
66794	kanosamine biosynthesis II	50	1 of 2
66794	ribulose monophosphate pathway	50	1 of 2
66794	phenylmercury acetate degradation	50	1 of 2
66794	glutathione metabolism	50	7 of 14
66794	glycogen biosynthesis	50	2 of 4
66794	butanoate fermentation	50	2 of 4
66794	ascorbate metabolism	45.45	10 of 22
66794	vitamin B6 metabolism	45.45	5 of 11
66794	androgen and estrogen metabolism	43.75	7 of 16
66794	tyrosine metabolism	42.86	6 of 14
66794	reductive acetyl coenzyme A pathway	42.86	3 of 7
66794	bacilysin biosynthesis	40	2 of 5
66794	4-hydroxyphenylacetate degradation	40	4 of 10
66794	gallate degradation	40	2 of 5
66794	arachidonate biosynthesis	40	2 of 5
66794	phenylacetate degradation (aerobic)	40	2 of 5
66794	D-cycloserine biosynthesis	40	2 of 5
66794	myo-inositol biosynthesis	40	4 of 10
66794	ethylmalonyl-CoA pathway	40	2 of 5
66794	3-chlorocatechol degradation	40	2 of 5
66794	phenylpropanoid biosynthesis	38.46	5 of 13
66794	allantoin degradation	33.33	3 of 9
66794	octane oxidation	33.33	1 of 3
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	selenocysteine biosynthesis	33.33	2 of 6
66794	lipid A biosynthesis	33.33	3 of 9
66794	degradation of hexoses	33.33	6 of 18
66794	pantothenate biosynthesis	33.33	2 of 6
66794	IAA biosynthesis	33.33	1 of 3
66794	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
66794	coenzyme M biosynthesis	30	3 of 10
66794	benzoyl-CoA degradation	28.57	2 of 7
66794	ubiquinone biosynthesis	28.57	2 of 7
66794	arachidonic acid metabolism	27.78	5 of 18
66794	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
66794	3-phenylpropionate degradation	26.67	4 of 15
66794	polyamine pathway	26.09	6 of 23
66794	CMP-KDO biosynthesis	25	1 of 4
66794	phenol degradation	25	5 of 20
66794	toluene degradation	25	1 of 4
66794	cyclohexanol degradation	25	1 of 4
66794	lactate fermentation	25	1 of 4
66794	carotenoid biosynthesis	22.73	5 of 22
66794	nitrate assimilation	22.22	2 of 9

API coryne

@ref	Reduction of nitrateNIT	PYZ	PYRA	PAL	beta GUR	beta GAL	alpha GLU	beta NAG	ESC	URE	GEL	Control fermentationControl	GLU	RIB	XYL	MAN	MAL	LAC	SAC	GLYG	CAT
18519	not determinedn.d.	+	+	-	-	+	+	-	+	+/-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	-	not determinedn.d.	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
18519	not determinedn.d.	-	+	+	-	+	+	+	-	-	+	+	+	+	-	+	+	+	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Fungi	#Mushroom

Isolation

@ref	Sample type	Host species	Geographic location	Country	Country ISO 3 Code	Continent
5742	cultivated mushroom Pleurotus ostreatus	Pleurotus ostreatus		Japan	JPN	Asia
67770	Fruiting body of rotting cultivated mushroom Pleurotusostreatus	Pleurotusostreatus	Gifu Pref.	Japan	JPN	Asia

Taxonmaps

69479

Global distribution of 16S sequence AM410678 (>99% sequence identity) for Microbacterium from Microbeatlas

Aquatic Samples	298
Soil Samples	136
Animal Samples	351
Plant Samples	258
Total Samples	1043

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
18519	1	Risk group (German classification) According to TRBA 466
5742	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM366757v1 assembly for Mycetocola lacteus JCM 11654	contig	GCA_003667575	76637.3	2832382567	76637	64.32

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Mycetocola lacteus partial 16S rRNA gene, type strain DSM 15177	AM410678	1445		76637
5742	Mycetocola lacteus gene for 16S rRNA	AB012648	1445		76637

GC content

@ref	GC-content (mol%)	Method
5742	64	high performance liquid chromatography (HPLC)
67770	63.9	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Mycetocola lacteus JCM 11654
NCBI: GCA_003667575

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	89.80	no
125439	motility	BacteriaNetⓘ	no	57.60	no
125439	gram_stain	BacteriaNetⓘ	negative	71.70	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	65.10	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Mycetocola lacteus strain JCM 11654
PATRIC: 76637.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	91.99	no
125438	anaerobic	anaerobicⓘ	no	95.23	no
125438	aerobic	aerobicⓘ	yes	81.96	no
125438	spore-forming	spore-formingⓘ	no	80.46	no
125438	thermophilic	thermophileⓘ	no	95.98	yes
125438	flagellated	motile2+ⓘ	no	91.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Mycetocola miduiensis sp. nov., a psychrotolerant bacterium isolated from Midui glacier.	Zhu L, Liu Q, Liu H, Zhou Y, Xin Y, Dong X	Int J Syst Evol Microbiol	10.1099/ijs.0.047985-0	2013
Phylogeny	Proposal of Mycetocola gen. nov. in the family Microbacteriaceae and three new species, Mycetocola saprophilus sp. nov., Mycetocola tolaasinivorans sp. nov. and Mycetocola lacteus sp. nov., isolated from cultivated mushroom, Pleurotus ostreatus.	Tsukamoto T, Takeuchi M, Shida O, Murata H, Shirata A	Int J Syst Evol Microbiol	10.1099/00207713-51-3-937	2001

References

#5742	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15177
#18519	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68379	Automatically annotated from API Coryne .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Mycetocola lacteus (DSM 15177, IFO 16278, NBRC 16278)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Observation

Metabolite utilization

Enzymes

Pathway annotation

API coryne

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_003667575

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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