Name: Mycetocola saprophilus CM-01
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 7469

Type strain:

Species: Mycetocola saprophilus

Strain Designation: CM-01

Culture col. no.: DSM 15178, IFO 16274, NBRC 16274, NRRL B-24119, JCM 11655, CGMCC 1.10735, IAM 15092, LMG 22188, MAFF 211324

Strain history: IFO 16274 <-- T. Tsukamoto CM-01.

NCBI tax ID(s): 76636 (species)

SI-ID 62363

IFO 16274, DSM 15178, NRRL B-24119, JCM 11655, NBRC 16274, LMG 22188, IAM 15092

Section

Mycetocola saprophilus CM-01 is a mesophilic bacterium that was isolated from cultivated mushroom Pleurotus ostreatus.

mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Microbacteriaceae*
	Genus	*Mycetocola*
	Species	**Mycetocola saprophilus**
	Full Scientific Name (LPSN)	Mycetocola saprophilus Tsukamoto et al. 2001

Information on morphological and physiological properties Morphology

[Ref.: #18520]	Colony color	Beige (1001)
[Ref.: #18520]	Incubation period	10-14 days
[Ref.: #18520]	Cultivation medium used	ISP 2

[Ref.: #18520]	Colony color	Beige (1001)
[Ref.: #18520]	Incubation period	10-14 days
[Ref.: #18520]	Cultivation medium used	ISP 3

[Ref.: #18520]	Incubation period	10-14 days
[Ref.: #18520]	Cultivation medium used	ISP 4

[Ref.: #18520]	Incubation period	10-14 days
[Ref.: #18520]	Cultivation medium used	ISP 5

[Ref.: #18520]	Incubation period	10-14 days
[Ref.: #18520]	Cultivation medium used	ISP 6

[Ref.: #18520]	Incubation period	10-14 days
[Ref.: #18520]	Cultivation medium used	ISP 7

[Ref.: #18520]	Multicellular complex forming ability	no
[Ref.: #18520]	Medium Name (multicellularity)	ISP 2

[Ref.: #18520]	Multicellular complex forming ability	no
[Ref.: #18520]	Medium Name (multicellularity)	ISP 3

[Ref.: #18520]	Multicellular complex forming ability	no
[Ref.: #18520]	Medium Name (multicellularity)	ISP 4

[Ref.: #18520]	Multicellular complex forming ability	no
[Ref.: #18520]	Medium Name (multicellularity)	ISP 5

[Ref.: #18520]	Multicellular complex forming ability	no
[Ref.: #18520]	Medium Name (multicellularity)	ISP 6

[Ref.: #18520]	Multicellular complex forming ability	no
[Ref.: #18520]	Medium Name (multicellularity)	ISP 7

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5743]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

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Culture medium

ISP 2

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Culture medium growth

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Culture medium composition

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Culture medium

ISP 3

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Culture medium growth

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Culture medium composition

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Culture medium

ISP 4

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Culture medium growth

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Culture medium composition

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Culture medium

ISP 5

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Culture medium growth

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Culture medium composition

expand / minimize

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Culture medium

ISP 6

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Culture medium growth

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Culture medium composition

expand / minimize

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Culture medium

ISP 7

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Culture medium growth

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Culture medium composition

expand / minimize

		Temperature
[Ref.: #5743]	growth	28 °C
[Ref.: #18520]	optimum	28 °C
[Ref.: #67770]	growth	30 °C

[Ref.: #5743]	Temperature range	mesophilic
[Ref.: #18520]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #67770]

Observation

quinones: MK-10, MK-9, MK-11

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #18520]	arabinose ChEBI	-
[Ref.: #18520]	cellulose ChEBI	-
[Ref.: #68379]	esculin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #18520]	fructose ChEBI	+/-
[Ref.: #68379]	gelatin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #18520]	glucose ChEBI	-
[Ref.: #18520]	mannitol ChEBI	+/-
[Ref.: #18520]	myo-inositol ChEBI	-
[Ref.: #18520]	raffinose ChEBI	-
[Ref.: #18520]	rhamnose ChEBI	-
[Ref.: #18520]	sucrose ChEBI	+/-
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #18520]	xylose ChEBI	+
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym, API Coryne
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym, API Coryne
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym, API Coryne
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68379]	beta-glucosidase	-	3.2.1.21	* from API Coryne
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym, API Coryne
[Ref.: #68382]	cystine arylamidase	+	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68379]	gelatinase	-		* from API Coryne
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API Coryne
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #68379]	pyrazinamidase	+	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	+	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #18520]	154	not determinedn.d.	+	+	-	-	+	+	-	-	+/-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	-	not determinedn.d.	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #18520]	213	not determinedn.d.	-	+	+	-	+	+	+	-	-	-	-	+	+	-	+	+	+	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5743]	Sample type/isolated from	cultivated mushroom Pleurotus ostreatus
[Ref.: #5743]	Host species	Pleurotus ostreatus
[Ref.: #5743]	Country	Japan
[Ref.: #5743]	Country ISO 3 Code	JPN
[Ref.: #5743]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Fruiting body of rotting cultivated mushroom Pleurotusostreatus
[Ref.: #67770]	Host species	Pleurotusostreatus
[Ref.: #67770]	Geographic location (country and/or sea, region)	Yamagata Pref.
[Ref.: #67770]	Country	Japan
[Ref.: #67770]	Country ISO 3 Code	JPN
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Host

#Fungi

#Mushroom

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AM410677 (>99% sequence identity) for Microbacterium from Microbeatlas

Aquatic Samples	298
Soil Samples	136
Animal Samples	351
Plant Samples	258
Total Samples	1043

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #18520]	Biosafety level	1	Risk group (German classification)
[Ref.: #5743]	Biosafety level	1	Risk group (German classification) According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #5743]	Mycetocola saprophilus gene for 16S rRNA	AB012647	1444	ENA	76636 tax ID
[Ref.: #20218]	Mycetocola saprophilus partial 16S rRNA gene, type strain DSM 15178	AM410677	1444	ENA	76636 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Mycetocola saprophilus NRRL B-24119	GCA_000718085	contig	GenBank	76636 tax ID

[Ref.: #5743]	GC-content	65.2 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67770]	GC-content	66.1 mol%	genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Mycetocola saprophilus NRRL B-24119 NCBI: GCA_000718085.1
[Ref.: #69480]	motile	no	93.357	no
[Ref.: #69480]	flagellated	no	97.187	no
[Ref.: #69480]	gram-positive	yes	94.288	no
[Ref.: #69480]	anaerobic	no	98.772	no
[Ref.: #69480]	halophile	no	78.545	no
[Ref.: #69480]	spore-forming	no	93.194	no
[Ref.: #69480]	glucose-util	yes	62.891	yes
[Ref.: #69480]	aerobic	yes	89.671	no
[Ref.: #69480]	thermophile	no	99.076	yes
[Ref.: #69480]	glucose-ferment	no	81.839	no

Availability in culture collections External links

[Ref.: #5743]

Culture collection no.

DSM 15178, IFO 16274, NBRC 16274, NRRL B-24119, JCM 11655, CGMCC 1.10735, IAM 15092, LMG 22188, MAFF 211324

[Ref.: #76914]

SI-ID 62363

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Mycetocola spongiae sp. nov., isolated from deep-sea sponge Cacospongia mycofijiensis.	Chen Y, Sang J, Sun W, Song Q, Li Z	Int J Syst Evol Microbiol	10.1099/ijsem.0.005291	2022	*
Phylogeny	Mycetocola miduiensis sp. nov., a psychrotolerant bacterium isolated from Midui glacier.	Zhu L, Liu Q, Liu H, Zhou Y, Xin Y, Dong X	Int J Syst Evol Microbiol	10.1099/ijs.0.047985-0	2013	*
Phylogeny	Proposal of Mycetocola gen. nov. in the family Microbacteriaceae and three new species, Mycetocola saprophilus sp. nov., Mycetocola tolaasinivorans sp. nov. and Mycetocola lacteus sp. nov., isolated from cultivated mushroom, Pleurotus ostreatus.	Tsukamoto T, Takeuchi M, Shida O, Murata H, Shirata A	Int J Syst Evol Microbiol	10.1099/00207713-51-3-937	2001	*

References

#5743

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15178

#18520

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#76914

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID62363.1 )

* These data were automatically processed and therefore are not curated

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Mycetocola saprophilus NRRL B-24119

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