Microbacterium trichothecenolyticum (DSM 8608, ATCC 51475, CCM 4373)

Name: Microbacterium trichothecenolyticum (DSM 8608, ATCC 51475, CCM 4373)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7398

Type strain

Strain Designation 114-2

Culture col. no. DSM 8608 ATCC 51475 CCM 4373 CIP 103817 IFO 15077 7 more

NCBI tax ID(s) 69370

Special Collections DSMZ JCM CIP Wink compendium

History <- IFO <- JCM <- K. Nakayama, 114-2 K. Nakayama 114-2. CIP <- 1993, IFO <- 1990, JCM <- K. Nakayama: strain 114-2

Links

Microbacterium trichothecenolyticum 114-2 is an obligate aerobe, Gram-positive, rod-shaped bacterium that was isolated from soil.

Gram-positive rod-shaped obligate aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 8211

LMG 16696,ATCC 51475,CCM 4373,CIP 103817,IFO 15077,JCM 1358,DSM 8608,VKM Ac-1948,NBRC 15077,NRRL B-24212,IAM 15206

@ref 20215

Last LPSN update 2026-02-09 11:19:28

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Micrococcales

Family Microbacteriaceae

Genus Microbacterium

Species Microbacterium trichothecenolyticum

Full scientific name Microbacterium trichothecenolyticum (Yokota et al. 1993) Takeuchi and Hatano 1998

Synonyms (1)

Aureobacterium trichothecenolyticum

BacDive ID	Other strains from **Microbacterium trichothecenolyticum** (1)	Type strain
100778	M. trichothecenolyticum ST033251(HKI),

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
123583	positive	rod-shaped
125438			93.5
125438	positive		92.624
125439	positive		94.5

Colony morphology

@ref	Colony color	Incubation period	Medium used
20207	Beige (1001)	10-14 days	ISP 2
20207	Beige (1001)	10-14 days	ISP 3
20207	Beige (1001)	10-14 days	ISP 4
20207	Beige (1001)	10-14 days	ISP 5
20207	Lemon yellow (1012)	10-14 days	ISP 6
20207	Beige (1001)	10-14 days	ISP 7
123583

Multicellular morphology

@ref	Forms multicellular complex	Medium name
20207		ISP 2
20207		ISP 3
20207		ISP 4
20207		ISP 5
20207		ISP 6
20207		ISP 7

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
3330	TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)	Medium recipe at MediaDive	Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
20207	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
20207	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
20207	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
20207	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
20207	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
20207	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
39859	MEDIUM 3 - Columbia agar		Columbia agar (39.000 g);distilled water (1000.000 ml)
123583	CIP Medium 3	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
3330	positive	growth	30
20207	positive	optimum	28
39859	positive	growth	30
67770	positive	growth	30
123583	positive	growth	15-37
123583	negative	growth	10
123583	negative	growth	41
123583	negative	growth	45

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
123583	obligate aerobe
125439	obligate aerobe	99.7

Halophily

@ref	Salt	Growth	Tested relation	Concentration
123583	NaCl	positive	growth	0-4 %
123583	NaCl		growth	6 %
123583	NaCl		growth	8 %
123583	NaCl		growth	10 %

Murein

@ref	Murein short key	Type
3330	B06	B2ß {Gly} [L-Hsr] D-Glu(Hyg)-Gly-D-Orn

Observation

67770

Observationquinones: MK-12, MK-13

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
123583	16947 ChEBI	citrate	+	carbon source
68379	17634 ChEBI	D-glucose	-	fermentation	from API Coryne
68379	16899 ChEBI	D-mannitol	-	fermentation	from API Coryne
68379	16988 ChEBI	D-ribose	-	fermentation	from API Coryne
68379	65327 ChEBI	D-xylose	-	fermentation	from API Coryne
68379	4853 ChEBI	esculin	+	hydrolysis	from API Coryne
123583	4853 ChEBI	esculin	+	hydrolysis
68379	5291 ChEBI	gelatin	+	hydrolysis	from API Coryne
68379	28087 ChEBI	glycogen	-	fermentation	from API Coryne
123583	606565 ChEBI	hippurate	+	hydrolysis
68379	17716 ChEBI	lactose	-	fermentation	from API Coryne
68379	17306 ChEBI	maltose	-	fermentation	from API Coryne
68379	17632 ChEBI	nitrate	-	reduction	from API Coryne
123583	17632 ChEBI	nitrate	+	reduction
123583	17632 ChEBI	nitrate	-	respiration
123583	16301 ChEBI	nitrite	-	reduction
123583	132112 ChEBI	sodium thiosulfate	+	builds gas from
68379	17992 ChEBI	sucrose	-	fermentation	from API Coryne
123583	35020 ChEBI	tributyrin	-	hydrolysis
68379	16199 ChEBI	urea	+	hydrolysis	from API Coryne

Antibiotic resistance

@ref	Metabolite	Is sensitive	Is resistant
123583	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)

Metabolite production

@ref	Chebi-ID	Metabolite	Production
123583	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test
123583	15688 ChEBI	acetoin	-
123583	17234 ChEBI	glucose		-

Enzymes

@ref	Value	Activity	Ec
123583	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68379	alkaline phosphatase	-	3.1.3.1	from API Coryne
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68379	alpha-glucosidase	-	3.2.1.20	from API Coryne
123583	amylase	+
68382	beta-galactosidase	+	3.2.1.23	from API zym
123583	beta-galactosidase	+	3.2.1.23
68379	beta-galactosidase	-	3.2.1.23	from API Coryne
68382	beta-glucosidase	+	3.2.1.21	from API zym
68379	beta-glucosidase	+	3.2.1.21	from API Coryne
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68379	beta-glucuronidase	-	3.2.1.31	from API Coryne
123583	caseinase	+	3.4.21.50
123583	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
123583	DNase	+
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
123583	gamma-glutamyltransferase	-	2.3.2.2
123583	gelatinase	+/-
68379	gelatinase	+		from API Coryne
123583	lecithinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
123583	lipase	-
123583	lysine decarboxylase	-	4.1.1.18
68379	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API Coryne
123583	ornithine decarboxylase	-	4.1.1.17
123583	oxidase	-
123583	phenylalanine ammonia-lyase	-	4.3.1.24
123583	protease	-
68379	pyrazinamidase	-	3.5.1.B15	from API Coryne
68379	pyrrolidonyl arylamidase	-	3.4.19.3	from API Coryne
123583	tryptophan deaminase	-
123583	urease	-	3.5.1.5
68379	urease	+	3.5.1.5	from API Coryne
68382	valine arylamidase	+		from API zym

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	ketogluconate metabolism	100	8 of 8
66794	phenylacetate degradation (aerobic)	100	5 of 5
66794	pentose phosphate pathway	100	11 of 11
66794	kanosamine biosynthesis II	100	2 of 2
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	threonine metabolism	100	10 of 10
66794	gallate degradation	100	5 of 5
66794	adipate degradation	100	2 of 2
66794	acetate fermentation	100	4 of 4
66794	lipoate biosynthesis	100	5 of 5
66794	palmitate biosynthesis	100	22 of 22
66794	ppGpp biosynthesis	100	4 of 4
66794	methylglyoxal degradation	100	5 of 5
66794	starch degradation	100	10 of 10
66794	butanoate fermentation	100	4 of 4
66794	ribulose monophosphate pathway	100	2 of 2
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	folate polyglutamylation	100	1 of 1
66794	suberin monomers biosynthesis	100	2 of 2
66794	formaldehyde oxidation	100	3 of 3
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	cellulose degradation	100	5 of 5
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	sulfopterin metabolism	100	4 of 4
66794	coenzyme A metabolism	100	4 of 4
66794	vitamin K metabolism	100	5 of 5
66794	photosynthesis	92.86	13 of 14
66794	phenylalanine metabolism	92.31	12 of 13
66794	propionate fermentation	90	9 of 10
66794	Entner Doudoroff pathway	90	9 of 10
66794	NAD metabolism	88.89	16 of 18
66794	valine metabolism	88.89	8 of 9
66794	allantoin degradation	88.89	8 of 9
66794	4-hydroxymandelate degradation	88.89	8 of 9
66794	d-mannose degradation	88.89	8 of 9
66794	chorismate metabolism	88.89	8 of 9
66794	gluconeogenesis	87.5	7 of 8
66794	flavin biosynthesis	86.67	13 of 15
66794	purine metabolism	86.17	81 of 94
66794	pyrimidine metabolism	84.44	38 of 45
66794	glycolysis	82.35	14 of 17
66794	glutamate and glutamine metabolism	82.14	23 of 28
66794	3-chlorocatechol degradation	80	4 of 5
66794	peptidoglycan biosynthesis	80	12 of 15
66794	glycogen metabolism	80	4 of 5
66794	ethylmalonyl-CoA pathway	80	4 of 5
66794	factor 420 biosynthesis	80	4 of 5
66794	tetrahydrofolate metabolism	78.57	11 of 14
66794	citric acid cycle	78.57	11 of 14
66794	heme metabolism	78.57	11 of 14
66794	aspartate and asparagine metabolism	77.78	7 of 9
66794	serine metabolism	77.78	7 of 9
66794	CO2 fixation in Crenarchaeota	77.78	7 of 9
66794	molybdenum cofactor biosynthesis	77.78	7 of 9
66794	leucine metabolism	76.92	10 of 13
66794	alanine metabolism	75.86	22 of 29
66794	glycogen biosynthesis	75	3 of 4
66794	toluene degradation	75	3 of 4
66794	isoleucine metabolism	75	6 of 8
66794	phenol degradation	75	15 of 20
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	C4 and CAM-carbon fixation	75	6 of 8
66794	tryptophan metabolism	73.68	28 of 38
66794	non-pathway related	73.68	28 of 38
66794	histidine metabolism	72.41	21 of 29
66794	degradation of sugar acids	72	18 of 25
66794	cardiolipin biosynthesis	71.43	5 of 7
66794	reductive acetyl coenzyme A pathway	71.43	5 of 7
66794	ubiquinone biosynthesis	71.43	5 of 7
66794	vitamin B1 metabolism	69.23	9 of 13
66794	methionine metabolism	69.23	18 of 26
66794	degradation of sugar alcohols	68.75	11 of 16
66794	degradation of pentoses	67.86	19 of 28
66794	glycolate and glyoxylate degradation	66.67	4 of 6
66794	enterobactin biosynthesis	66.67	2 of 3
66794	octane oxidation	66.67	2 of 3
66794	degradation of hexoses	66.67	12 of 18
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	methane metabolism	66.67	2 of 3
66794	acetoin degradation	66.67	2 of 3
66794	acetyl CoA biosynthesis	66.67	2 of 3
66794	isoprenoid biosynthesis	65.38	17 of 26
66794	tyrosine metabolism	64.29	9 of 14
66794	glutathione metabolism	64.29	9 of 14
66794	d-xylose degradation	63.64	7 of 11
66794	metabolism of disaccharids	63.64	7 of 11
66794	arginine metabolism	62.5	15 of 24
66794	dTDPLrhamnose biosynthesis	62.5	5 of 8
66794	urea cycle	61.54	8 of 13
66794	cysteine metabolism	61.11	11 of 18
66794	myo-inositol biosynthesis	60	6 of 10
66794	metabolism of amino sugars and derivatives	60	3 of 5
66794	4-hydroxyphenylacetate degradation	60	6 of 10
66794	propanol degradation	57.14	4 of 7
66794	oxidative phosphorylation	57.14	52 of 91
66794	androgen and estrogen metabolism	56.25	9 of 16
66794	lipid metabolism	54.84	17 of 31
66794	proline metabolism	54.55	6 of 11
66794	phosphatidylethanolamine bioynthesis	53.85	7 of 13
66794	3-phenylpropionate degradation	53.33	8 of 15
66794	lactate fermentation	50	2 of 4
66794	cyclohexanol degradation	50	2 of 4
66794	quinate degradation	50	1 of 2
66794	ethanol fermentation	50	1 of 2
66794	lysine metabolism	50	21 of 42
66794	glycine metabolism	50	5 of 10
66794	resorcinol degradation	50	1 of 2
66794	phenylmercury acetate degradation	50	1 of 2
66794	sulfate reduction	46.15	6 of 13
66794	ascorbate metabolism	45.45	10 of 22
66794	vitamin B6 metabolism	45.45	5 of 11
66794	lipid A biosynthesis	44.44	4 of 9
66794	nitrate assimilation	44.44	4 of 9
66794	polyamine pathway	43.48	10 of 23
66794	benzoyl-CoA degradation	42.86	3 of 7
66794	degradation of aromatic, nitrogen containing compounds	41.67	5 of 12
66794	glycine betaine biosynthesis	40	2 of 5
66794	arachidonate biosynthesis	40	2 of 5
66794	chlorophyll metabolism	38.89	7 of 18
66794	dolichyl-diphosphooligosaccharide biosynthesis	36.36	4 of 11
66794	cyanate degradation	33.33	1 of 3
66794	selenocysteine biosynthesis	33.33	2 of 6
66794	pantothenate biosynthesis	33.33	2 of 6
66794	IAA biosynthesis	33.33	1 of 3
66794	carotenoid biosynthesis	31.82	7 of 22
66794	coenzyme M biosynthesis	30	3 of 10
66794	bile acid biosynthesis, neutral pathway	29.41	5 of 17
66794	mevalonate metabolism	28.57	2 of 7
66794	arachidonic acid metabolism	27.78	5 of 18
66794	biotin biosynthesis	25	1 of 4
66794	CMP-KDO biosynthesis	25	1 of 4
66794	carnitine metabolism	25	2 of 8
66794	catecholamine biosynthesis	25	1 of 4

API coryne

@ref	Reduction of nitrateNIT	PYZ	PYRA	PAL	beta GUR	beta GAL	alpha GLU	beta NAG	ESC	URE	GEL	Control fermentationControl	GLU	RIB	XYL	MAN	MAL	LAC	SAC	GLYG	CAT
20207	-	-	-	-	-	-	-	-	+	+	+	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
20207	not determinedn.d.	-	+	+	+	+	+	-	+	-	-	-	+	+	-	+	+	-	+	-
123583	-	-	+	+	-	+	+	-	-	-	+	+	+	+	-	+	+	+	-	-

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
123583	not determinedn.d.	+	+	-	+	+	-	+	+	+	+	+	-	-	-	-	+	+	+	+	-	+	+	+	-	-	+	-	-	-	-	-	+	+	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	+	-	+	+	-	-	+	+	-	-	-	-	-	-	-	-	-	-	+	-	-	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type
3330	soil
67770	Soil
123583	Environment, Soil

Taxonmaps

69479

Global distribution of 16S sequence Y17240 (>99% sequence identity) for Microbacterium from Microbeatlas

Aquatic Samples	21
Soil Samples	125
Animal Samples	83
Plant Samples	108
Total Samples	337

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
3330	1	Risk group (German classification) According to TRBA 466
20207	1	German classification According to TRBA 466
123583	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM95646v1 assembly for Microbacterium trichothecenolyticum DSM 8608	contig	GCA_000956465	69370.6	2698536670	69370	70.55

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Microbacterium trichotecenolyticum 16S rRNA gene	Y17240	1461		69370
20218	Microbacterium trichotecenolyticum gene for 16S rRNA, partial sequence	AB004722	1463		69370

GC content

@ref	GC-content (mol%)	Method
3330	69	high performance liquid chromatography (HPLC)
67770	70.2	genome sequence analysis
67770	70.1	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Microbacterium trichothecenolyticum DSM 8608
NCBI: GCA_000956465

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	88.40	no
125439	motility	BacteriaNetⓘ	no	56.80	no
125439	gram_stain	BacteriaNetⓘ	positive	94.50	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	99.70	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Microbacterium trichothecenolyticum strain DSM 8608
PATRIC: 69370.6

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	92.62	no
125438	anaerobic	anaerobicⓘ	no	97.57	no
125438	spore-forming	spore-formingⓘ	no	79.25	no
125438	aerobic	aerobicⓘ	yes	89.68	no
125438	thermophilic	thermophileⓘ	no	97.88	yes
125438	flagellated	motile2+ⓘ	no	93.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	An Isolated Arthrobacter sp. Enhances Rice (Oryza sativa L.) Plant Growth.	Chhetri G, Kim I, Kang M, So Y, Kim J, Seo T.	Microorganisms	10.3390/microorganisms10061187	2022
Phylogeny	Phenotypic and genotypic properties of Microbacterium yannicii, a recently described multidrug resistant bacterium isolated from a lung transplanted patient with cystic fibrosis in France.	Sharma P, Diene SM, Thibeaut S, Bittar F, Roux V, Gomez C, Reynaud-Gaubert M, Rolain JM.	BMC Microbiol	10.1186/1471-2180-13-97	2013
	Efficient Degradation for Raffinose and Stachyose of a beta-D-Fructofuranosidase and Its New Function to Improve Gel Properties of Coagulated Fermented-Soymilk.	Chen Z, Shen Y, Xu J.	Gels	10.3390/gels9040345	2023
Pathogenicity	Case Series: Microbacterium aurum Bacteremia in Immunosuppressed Patients-An Emerging Threat.	Varghese G, Sarawat D, Jamwal A, Patel SS, Tejan N, Sahu C.	Am J Trop Med Hyg	10.4269/ajtmh.23-0701	2024
	Microbial degradation and community structure analysis of hydroxyl-terminated polybutadiene (HTPB).	Zhang Y, Zou M, Lodhi AF, Deng YL.	AMB Express	10.1186/s13568-021-01334-1	2021
	Differences of airborne and mural microorganisms in a 1,500-year-old Xu Xianxiu's Tomb, Taiyuan, China.	Liu J, Wu F, Xiang T, Ma W, He D, Zhang Q, Wang W, Duan Y, Tian T, Feng H.	Front Microbiol	10.3389/fmicb.2023.1253461	2023
Enzymology	Characterization of a Pyranose Oxidase/C-Glycoside Oxidase from Microbacterium sp. 3H14, Belonging to the Unexplored Clade II of Actinobacterial POx/CGOx.	Martschini A, Kostelac A, Haltrich D, Peterbauer CK.	Biomolecules	10.3390/biom14121510	2024
Enzymology	Shifting the substrate scope of dimeric pyranose oxidase from monosaccharide to glycoside preference through oligomeric state modification.	Kostelac A, Hermann E, Peterbauer C, Oostenbrink C, Haltrich D.	FEBS J	10.1111/febs.70004	2025
Enzymology	Biochemical Characterization of Pyranose Oxidase from Streptomyces canus-Towards a Better Understanding of Pyranose Oxidase Homologues in Bacteria.	Kostelac A, Sutzl L, Puc J, Furlanetto V, Divne C, Haltrich D.	Int J Mol Sci	10.3390/ijms232113595	2022
	Expression and characterization of a protease-resistant beta-d-fructofuranosidase BbFFase9 gene suitable for preparing invert sugars from soybean meal.	Chen Z, Shen Y, Wang R, Li S, Jia Y.	Heliyon	10.1016/j.heliyon.2023.e19889	2023
	Mercury alters the rhizobacterial community in Brazilian wetlands and it can be bioremediated by the plant-bacteria association.	Mariano C, Mello IS, Barros BM, da Silva GF, Terezo AJ, Soares MA.	Environ Sci Pollut Res Int	10.1007/s11356-020-07913-2	2020
	A Strategy for Rapid Acquisition of the beta-D-Fructofuranosidase Gene through Chemical Synthesis and New Function of Its Encoded Enzyme to Improve Gel Properties during Yogurt Processing.	Chen Z, Shen Y, Xu J.	Foods	10.3390/foods12081704	2023
Enzymology	Evolution and separation of actinobacterial pyranose and C-glycoside-3-oxidases.	Kostelac A, Taborda A, Martins LO, Haltrich D.	Appl Environ Microbiol	10.1128/aem.01676-23	2024
Phylogeny	Heterotrophic Microbiota from the Oligotrophic Waters of Lake Vostok, Antarctica.	Epova EY, Shevelev AB, Akbayev RM, Biryukova YK, Zylkova MV, Bogdanova ES, Guseva MA, Tynio YY, Egorov VV.	Int J Environ Res Public Health	10.3390/ijerph19074025	2022
Metabolism	Ginsenoside Rb1 is transformed into Rd and Rh2 by Microbacterium trichothecenolyticum.	Kim H, Kim JH, Lee PY, Bae KH, Cho S, Park BC, Shin H, Park SG.	J Microbiol Biotechnol	10.4014/jmb.1307.07049	2013
Enzymology	Identification of a robust bacterial pyranose oxidase that displays an unusual pH dependence.	Santema LL, Rozeboom HJ, Borger VP, Kaya SG, Fraaije MW.	J Biol Chem	10.1016/j.jbc.2024.107885	2024
	Identification of an Invertase With High Specific Activity for Raffinose Hydrolysis and Its Application in Soymilk Treatment.	Liu J, Cheng J, Huang M, Shen C, Xu K, Xiao Y, Pan W, Fang Z.	Front Microbiol	10.3389/fmicb.2021.646801	2021
	Microbial network and fermentation modulation of Napier grass and sugarcane top silage in southern Africa.	Du Z, Yamasaki S, Oya T, Nguluve D, Euridse D, Tinga B, Macome F, Cai Y.	Microbiol Spectr	10.1128/spectrum.03032-23	2024
Metabolism	C-Glycoside metabolism in the gut and in nature: Identification, characterization, structural analyses and distribution of C-C bond-cleaving enzymes.	Mori T, Kumano T, He H, Watanabe S, Senda M, Moriya T, Adachi N, Hori S, Terashita Y, Kawasaki M, Hashimoto Y, Awakawa T, Senda T, Abe I, Kobayashi M.	Nat Commun	10.1038/s41467-021-26585-1	2021
	Microbial Co-occurrence Network and Fermentation Information of Natural Woody-Plant Silage Prepared With Grass and Crop By-Product in Southern Africa.	Du Z, Yamasaki S, Oya T, Nguluve D, Euridse D, Tinga B, Macome F, Cai Y.	Front Microbiol	10.3389/fmicb.2022.756209	2022
	Characterisation of sequence-structure-function space in sensor-effector integrators of phytochrome-regulated diguanylate cyclases.	Bohm C, Gourinchas G, Zweytick S, Hujdur E, Reiter M, Trstenjak S, Sensen CW, Winkler A.	Photochem Photobiol Sci	10.1007/s43630-022-00255-7	2022
Enzymology	Bacteremia caused by Microbacterium binotii in a patient with sickle cell anemia.	Buss SN, Starlin R, Iwen PC.	J Clin Microbiol	10.1128/jcm.02443-13	2014
Genetics	Four new Microbacterium species isolated from seaweeds and reclassification of five Microbacterium species with a proposal of Paramicrobacterium gen. nov. under a genome-based framework of the genus Microbacterium.	Lee SD, Yang HL, Kim IS.	Front Microbiol	10.3389/fmicb.2023.1299950	2023
Metabolism	d-Lactic acid secreted by Chlorella fusca primes pattern-triggered immunity against Pseudomonas syringae in Arabidopsis.	Lee SM, Kim SK, Lee N, Ahn CY, Ryu CM.	Plant J	10.1111/tpj.14661	2020
Enzymology	Novel NAD+-Farnesal Dehydrogenase from Polygonum minus Leaves. Purification and Characterization of Enzyme in Juvenile Hormone III Biosynthetic Pathway in Plant.	Seman-Kamarulzaman AF, Mohamed-Hussein ZA, Ng CL, Hassan M.	PLoS One	10.1371/journal.pone.0161707	2016
Phylogeny	In vitro fermentation of arabinoxylan from oat (Avena sativa L.) by Pekin duck intestinal microbiota.	Tian D, Xu X, Peng Q, Wen Z, Zhang Y, Wei C, Qiao Y, Shi B.	3 Biotech	10.1007/s13205-019-1571-5	2019
	Exploring the potentialities of beneficial endophytes for improved plant growth.	Fadiji AE, Babalola OO.	Saudi J Biol Sci	10.1016/j.sjbs.2020.08.002	2020
Metabolism	FAD-dependent C-glycoside-metabolizing enzymes in microorganisms: Screening, characterization, and crystal structure analysis.	Kumano T, Hori S, Watanabe S, Terashita Y, Yu HY, Hashimoto Y, Senda T, Senda M, Kobayashi M.	Proc Natl Acad Sci U S A	10.1073/pnas.2106580118	2021
Phylogeny	Union of the genera Microbacterium Orla-Jensen and Aureobacterium Collins et al. in a redefined genus Microbacterium.	Takeuchi M, Hatano K.	Int J Syst Bacteriol	10.1099/00207713-48-3-739	1998
Phylogeny	Coaggregation occurs amongst bacteria within and between biofilms in domestic showerheads.	Vornhagen J, Stevens M, McCormick DW, Dowd SE, Eisenberg JN, Boles BR, Rickard AH.	Biofouling	10.1080/08927014.2012.744395	2013
	Characterization of the plant growth promoting bacterium, Enterobacter cloacae MSR1, isolated from roots of non-nodulating Medicago sativa.	Khalifa AY, Alsyeeh AM, Almalki MA, Saleh FA.	Saudi J Biol Sci	10.1016/j.sjbs.2015.06.008	2016
Metabolism	Emulsifying activity and stability of a non-toxic bioemulsifier synthesized by Microbacterium sp. MC3B-10.	Camacho-Chab JC, Guezennec J, Chan-Bacab MJ, Rios-Leal E, Sinquin C, Muniz-Salazar R, De la Rosa-Garcia Sdel C, Reyes-Estebanez M, Ortega-Morales BO.	Int J Mol Sci	10.3390/ijms140918959	2013
	Impact of Soil Salinity on the Structure of the Bacterial Endophytic Community Identified from the Roots of Caliph Medic (Medicago truncatula).	Yaish MW, Al-Lawati A, Jana GA, Vishwas Patankar H, Glick BR.	PLoS One	10.1371/journal.pone.0159007	2016
Phylogeny	Isolation and Characterization of Lake Erie Bacteria that Degrade the Cyanobacterial Microcystin Toxin MC-LR.	Thees A, Atari E, Birbeck J, Westrick JA, Huntley JF.	J Great Lakes Res	10.1016/j.jglr.2018.10.013	2019
Enzymology	Distinctive molecular and biochemical characteristics of a glycoside hydrolase family 20 beta-N-acetylglucosaminidase and salt tolerance.	Zhou J, Song Z, Zhang R, Liu R, Wu Q, Li J, Tang X, Xu B, Ding J, Han N, Huang Z.	BMC Biotechnol	10.1186/s12896-017-0358-1	2017
Genetics	Genome-Based Taxonomic Classification of the Phylum Actinobacteria.	Nouioui I, Carro L, Garcia-Lopez M, Meier-Kolthoff JP, Woyke T, Kyrpides NC, Pukall R, Klenk HP, Goodfellow M, Goker M.	Front Microbiol	10.3389/fmicb.2018.02007	2018
Phylogeny	Proposal of six new species in the genus Aureobacterium and transfer of Flavobacterium esteraromaticum Omelianski to the genus Aureobacterium as Aureobacterium esteraromaticum comb. nov.	Yokota A, Takeuchi M, Sakane T, Weiss N	Int J Syst Bacteriol	10.1099/00207713-43-3-555	1993
	Lysinibacillus timonensis sp. nov., Microbacterium timonense sp. nov., and Erwinia mediterraneensis sp. nov., three new species isolated from the human skin.	Ndiaye C, Lo CI, Bassene H, Raoult D, Lagier JC, Sokhna C.	New Microbes New Infect	10.1016/j.nmni.2019.100579	2019
	Bacteremia due to a novel Microbacterium species in a patient with leukemia and description of Microbacterium paraoxydans sp. nov.	Laffineur K, Avesani V, Cornu G, Charlier J, Janssens M, Wauters G, Delmee M.	J Clin Microbiol	10.1128/jcm.41.5.2242-2246.2003	2003
Phylogeny	Microbacterium helvum sp. nov., a novel actinobacterium isolated from cow dung.	Li X, Zhang L, Huang F, Zhao J, Wang H, Jiao Y, Qian L, Wang X, Xiang W	Arch Microbiol	10.1007/s00203-021-02311-9	2021
Phylogeny	Microbacterium hibisci sp. nov., isolated from rhizosphere of mugunghwa (Hibiscus syriacus L.).	Yan ZF, Lin P, Won KH, Yang JE, Li CT, Kook M, Wang QJ, Yi TH	Int J Syst Evol Microbiol	10.1099/ijsem.0.002167	2017
Phylogeny	Microbacterium aureliae sp. nov., a novel actinobacterium isolated from Aurelia aurita, the moon jellyfish.	Kaur G, Mual P, Kumar N, Verma A, Kumar A, Krishnamurthi S, Mayilraj S	Int J Syst Evol Microbiol	10.1099/ijsem.0.001407	2016
Phylogeny	Microbacterium kyungheense sp. nov. and Microbacterium jejuense sp. nov., isolated from salty soil.	Kook M, Son HM, Yi TH	Int J Syst Evol Microbiol	10.1099/ijs.0.054973-0	2014

References

#3330	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 8608
#20207	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#39859	; Curators of the CIP;
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68379	Automatically annotated from API Coryne .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#123583	Collection of Institut Pasteur ; Curators of the CIP; CIP 103817
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Microbacterium trichothecenolyticum (DSM 8608, ATCC 51475, CCM 4373)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Halophily

Murein

Observation

Metabolite utilization

Antibiotic resistance

Metabolite production

Metabolite tests

Enzymes

Pathway annotation

API coryne

API zym

API biotype100

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000956465

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

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