Name: Leucobacter alluvii RB-10
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 7353

Type strain:

Species: Leucobacter alluvii

Strain Designation: RB-10

Culture col. no.: DSM 18279, CIP 108819, LMG 23117, RB 10, JCM 14919, UCCCB14

Strain history: CIP <- 2005, P. Morais, Coimbra Univ., Coimbra, Portugal: strain RB10

NCBI tax ID(s): 340321 (species)

SI-ID 139546

LMG 23117, CIP 108819, DSM 18279, JCM 14919

Section

Leucobacter alluvii RB-10 is an obligate aerobe, mesophilic, Gram-positive bacterium that was isolated from activated sludge, chromium polluted wastewater.

Gram-positive
rod-shaped
mesophilic
obligate aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Microbacteriaceae*
	Genus	*Leucobacter*
	Species	**Leucobacter alluvii**
	Full Scientific Name (LPSN)	Leucobacter alluvii Morais et al. 2006

Information on morphological and physiological properties Morphology

[Ref.: #116260]	Gram stain	positive

[Ref.: #116260]	Cell shape	rod-shaped

[Ref.: #116260]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7484]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #7484]

Culture medium growth

[Ref.: #7484]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7484]

Culture medium composition

expand / minimize

[Ref.: #39546]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #39546]

Culture medium growth

[Ref.: #39546]

Culture medium composition

expand / minimize

[Ref.: #116260]

Culture medium

CIP Medium 72

[Ref.: #116260]

Culture medium growth

[Ref.: #116260]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #7484]	growth	28 °C
[Ref.: #39546]	growth	30 °C
[Ref.: #67770]	growth	28 °C
[Ref.: #67772]	optimum	30 °C
[Ref.: #116260]	growth	30-37 °C

[Ref.: #7484]	Temperature range	mesophilic
[Ref.: #39546]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #67772]	Temperature range	mesophilic
[Ref.: #116260]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #67772]			optimum	7.0-8.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #116260]

Oxygen tolerance

obligate aerobe

[Ref.: #67770]

Observation

quinones: MK-11

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	cellobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #116260]	esculin ChEBI	-	hydrolysis
[Ref.: #68371]	esculin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	maltose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #116260]	nitrate ChEBI	-	reduction
[Ref.: #116260]	nitrate ChEBI	-	respiration
[Ref.: #116260]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #116260]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #116260]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #116260]	beta-galactosidase	+	3.2.1.23
[Ref.: #116260]	catalase	+	1.11.1.6
[Ref.: #116260]	gelatinase	+
[Ref.: #116260]	lysine decarboxylase	-	4.1.1.18
[Ref.: #116260]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #116260]	oxidase	-
[Ref.: #116260]	tween esterase	-
[Ref.: #116260]	urease	-	3.5.1.5

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #116260]	11393	not determinedn.d.	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7484]	Sample type/isolated from	activated sludge, chromium polluted wastewater
[Ref.: #7484]	Geographic location (country and/or sea, region)	Alcanena
[Ref.: #7484]	Country	Portugal
[Ref.: #7484]	Country ISO 3 Code	PRT
[Ref.: #7484]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	River Alviela sediments
[Ref.: #67770]	Country	Portugal
[Ref.: #67770]	Country ISO 3 Code	PRT
[Ref.: #67770]	Continent	Europe

[Ref.: #67772]	Sample type/isolated from	River sediments with 0.3% Cr(VI)
[Ref.: #67772]	Sampling date	1997
[Ref.: #67772]	Geographic location (country and/or sea, region)	Ribatejo, Alcanena
[Ref.: #67772]	Country	Portugal
[Ref.: #67772]	Country ISO 3 Code	PRT
[Ref.: #67772]	Continent	Europe
[Ref.: #67772]	Geographic location	39.4667°/-8.6667°

[Ref.: #116260]	Sample type/isolated from	Environment, River sediments with chromium
[Ref.: #116260]	Geographic location (country and/or sea, region)	Alcanena
[Ref.: #116260]	Country	Portugal
[Ref.: #116260]	Country ISO 3 Code	PRT
[Ref.: #116260]	Continent	Europe
[Ref.: #116260]	Isolation date	2003
* marker position based on {}

Isolation sources categories

#Engineered	#Contamination	#Heavy metal
#Engineered	#Waste	#Activated sludge
#Engineered	#Waste	#Wastewater

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AM072820 (>99% sequence identity) for Leucobacter from Microbeatlas

Aquatic Samples	43
Soil Samples	137
Animal Samples	392
Plant Samples	88
Total Samples	660

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7484]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #67772]	Biosafety level	1
[Ref.: #116260]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #7484]	Leucobacter alluvii partial 16S rRNA gene, type strain RB10T	AM072820	1525	ENA	340321 tax ID

[Ref.: #7484]	GC-content	68.9 mol%
[Ref.: #67770]	GC-content	68.9 mol%	high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #7484]

Culture collection no.

DSM 18279, CIP 108819, LMG 23117, RB 10, JCM 14919, UCCCB14

[Ref.: #76803]

SI-ID 139546

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Leucobacter iarius sp. nov., in the family Microbacteriaceae.	Somvanshi VS, Lang E, Schumann P, Pukall R, Kroppenstedt RM, Ganguly S, Stackebrandt E	Int J Syst Evol Microbiol	10.1099/ijs.0.64683-0	2007	*
Phylogeny	Leucobacter luti sp. nov., and Leucobacter alluvii sp. nov., two new species of the genus Leucobacter isolated under chromium stress.	Morais PV, Paulo C, Francisco R, Branco R, Paula Chung A, da Costa MS	Syst Appl Microbiol	10.1016/j.syapm.2005.10.005	2005	*

References

#7484

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 18279

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#39546 ; Curators of the CIP;
#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67772 The University of Coimbra Bacteria Culture Collection (UCCCB) ; Curators of the UCCCB;

#68371

Automatically annotated from API 50CH acid .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#76803

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID139546.1 )

#116260 Collection of Institut Pasteur ; Curators of the CIP; CIP 108819
* These data were automatically processed and therefore are not curated

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