Leucobacter tardus (DSM 19811, LMG 24388, K 70/01)

Name: Leucobacter tardus (DSM 19811, LMG 24388, K 70/01)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7354

Type strain

Strain Designation K 70/01

Culture col. no. DSM 19811 LMG 24388 K 70/01 JCM 16030

NCBI tax ID(s) 501483

Special Collections DSMZ JCM Wink compendium Literature strains

History <- U. Behrendt; K 70/01 LMG 24388 <-- U. Behrendt K 70/01.

Links

Leucobacter tardus K 70/01 is an aerobe, mesophilic, Gram-positive prokaryote that was isolated from phyllosphere of potato plants, Solanum tuberosum L..

Gram-positive rod-shaped aerobe mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 323087

LMG 24388,DSM 19811,JCM 16030

@ref 20215

Last LPSN update 2026-02-09 11:19:27

Domain Bacteria

Phylum Actinomycetota

Class Actinomycetes

Order Micrococcales

Family Microbacteriaceae

Genus Leucobacter

Species Leucobacter tardus

Full scientific name Leucobacter tardus Behrendt et al. 2008

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Confidence
31201	positive	0.8-1.6 µm	0.4-0.6 µm	rod-shaped
125438					92.75
125438	positive				90.399

Colony morphology

@ref	Colony color	Incubation period	Medium used
18615	Corn yellow (1006)	10-14 days	ISP 2
18615	Corn yellow (1006)	10-14 days	ISP 3
18615	Beige (1001)	10-14 days	ISP 4
18615	Corn yellow (1006)	10-14 days	ISP 5
18615	Corn yellow (1006)	10-14 days	ISP 6
18615	Corn yellow (1006)	10-14 days	ISP 7

Multicellular morphology

@ref	Forms multicellular complex	Medium name
18615		ISP 2
18615		ISP 3
18615		ISP 4
18615		ISP 5
18615		ISP 6
18615		ISP 7

Pigmentation

31201

Productionyes

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
8330	TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)	Medium recipe at MediaDive	Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
18615	ISP 2		Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
18615	ISP 3		Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
18615	ISP 4		Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
18615	ISP 5		Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
18615	ISP 6		Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
18615	ISP 7		Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
8330	positive	growth	28	mesophilic
18615	positive	optimum	28	mesophilic
31201	positive	growth	04-37
31201	positive	optimum	21-30
67770	positive	growth	28	mesophilic

Culture pH

@ref	Ability	Type	PH
31201	positive	optimum	7
31201	positive	growth	7

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
31201	aerobe
125439	obligate aerobe	99.1

Spore formation

@ref	Spore formation	Confidence
31201
125439		94

Observation

@ref	Observation
31201	aggregates in chains
67770	quinones: MK-10, MK-11, MK-9 (9:7:1)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
31201	22599 ChEBI	arabinose	+	carbon source
31201	4853 ChEBI	esculin	+	hydrolysis
31201	28757 ChEBI	fructose	+	carbon source
31201	24265 ChEBI	gluconate	+	carbon source
31201	17234 ChEBI	glucose	+	carbon source
31201	17754 ChEBI	glycerol	+	carbon source
31201	24996 ChEBI	lactate	+	carbon source
31201	29864 ChEBI	mannitol	+	carbon source
31201	15361 ChEBI	pyruvate	+	carbon source
31201	33942 ChEBI	ribose	+	carbon source
31201	17822 ChEBI	serine	+	carbon source
31201	30911 ChEBI	sorbitol	+	carbon source
31201	30031 ChEBI	succinate	+	carbon source
31201	53423 ChEBI	tween 40	+	carbon source
31201	53426 ChEBI	tween 80	+	carbon source
31201	18222 ChEBI	xylose	+	carbon source

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
31201	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
31201	cytochrome oxidase	+	1.9.3.1
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
68382	trypsin	-	3.4.21.4	from API zym
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
18615	not determinedn.d.	-	+	+	-	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Leaf (Phyllosphere)

Isolation

@ref	Sample type	Host species	Geographic location	Country	Country ISO 3 Code	Continent
8330	phyllosphere of potato plants, Solanum tuberosum L.	Solanum tuberosum	Dahnsdorf	Germany	DEU	Europe
67770	Phyllosphere of potato plants		Dahnsdorf	Germany	DEU	Europe

Taxonmaps

69479

Global distribution of 16S sequence AM940158 (>99% sequence identity) for Leucobacter tardus subclade from Microbeatlas

Aquatic Samples	148
Soil Samples	168
Animal Samples	453
Plant Samples	257
Total Samples	1026

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
8330	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM1758304v1 assembly for Leucobacter tardus K 70/01	contig	GCA_017583045	501483.3	2995723445	501483	66.67
124043	ASM3953183v1 assembly for Leucobacter tardus JCM 16030	scaffold	GCA_039531835			501483	50.96

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
8330	Leucobacter tardus partial 16S rRNA gene, type strain DSM 19811T	AM940158	1484		501483

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Leucobacter tardus K 70/01
NCBI: GCA_017583045

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	94.00	no
125439	motility	BacteriaNetⓘ	yes	60.70	no
125439	gram_stain	BacteriaNetⓘ	negative	85.80	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	99.10	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Leucobacter tardus K 70/01
NCBI: GCA_017583045.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	90.40	yes
125438	anaerobic	anaerobicⓘ	no	98.41	no
125438	aerobic	aerobicⓘ	yes	89.11	yes
125438	spore-forming	spore-formingⓘ	no	84.17	yes
125438	thermophilic	thermophileⓘ	no	96.49	yes
125438	flagellated	motile2+ⓘ	no	92.75	yes

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Leucobacter chromiisoli sp. nov., isolated from chromium-containing chemical plant soil.	Xu Z, Li X, Tian J, Gan L, Tian Y	Int J Syst Evol Microbiol	10.1099/ijsem.0.004923	2021
Phylogeny	Leucobacter ruminantium sp. nov., isolated from the bovine rumen.	Chun BH, Lee HJ, Jeong SE, Schumann P, Jeon CO	Int J Syst Evol Microbiol	10.1099/ijsem.0.002003	2017
Phylogeny	Leucobacter aerolatus sp. nov., from the air of a duck barn.	Martin E, Lodders N, Jackel U, Schumann P, Kampfer P	Int J Syst Evol Microbiol	10.1099/ijs.0.021303-0	2010
Phylogeny	Leucobacter tardus sp. nov., isolated from the phyllosphere of Solanum tuberosum L.	Behrendt U, Ulrich A, Schumann P	Int J Syst Evol Microbiol	10.1099/ijs.0.2008/001065-0	2008

References

#8330	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19811
#18615	Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#27528	IJSEM 2574 2008 ( DOI 10.1099/ijs.0.2008/001065-0 , PubMed 18984695 )
#31201	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27528
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Leucobacter tardus (DSM 19811, LMG 24388, K 70/01)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multicellular morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Observation

Metabolite utilization

Enzymes

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_017583045

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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