Name: Curtobacterium luteum 2Y-12
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 7315

Type strain:

Species: Curtobacterium luteum

Strain Designation: 2Y-12

Culture col. no.: DSM 20542, ATCC 15830, CCM 2298, NCIB 11029, CCUG 23848, IAM 1632, JCM 1480, BCRC 12127, CGMCC 1.1913, CIP 102694, IAM 15150, IAM 1623, IFO 12676, IMET 10360, KCCM 12167, KCTC 3493, LMG 8787, NBRC 12676, NCCB 71046, NCIMB 11029, VKM Ac-2188, VKM B-1210

Strain history: CIP <- 1986, NCIB <- K. Yamada: strain 2Y-12, Brevibacterium luteum

NCBI tax ID(s): 33881 (species)

SI-ID 264341

LMG 8787, ATCC 15830, CCM 2298, DSM 20542, IAM 1632, IFO 12676, NCIB 11029, CCUG 23848, CIP 102694, IAM 1623, IMET 10360, JCM 1480, CCRC 12127, NCIMB 11029, NBIMCC 1434, VKM B-1210, KCTC 3493, IAM 1622, NBRC 12676, NCCB 71046, LMD 71.46, BCRC 12127, CCT 3020, VKM Ac-2188, NCIM 2923, NCIM 2983, IAM 15150, CGMCC 1.1913, NCPPB 4112

Special Collections

Other strains from Curtobacterium luteum

Section

Curtobacterium luteum 2Y-12 is an aerobe, Gram-positive bacterium that was isolated from paddy.

Gram-positive
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Microbacteriaceae*
	Genus	*Curtobacterium*
	Species	**Curtobacterium luteum**
	Full Scientific Name (LPSN)	Curtobacterium luteum (Komagata and Iizuka 1964) Yamada and Komagata 1972 (Approved Lists 1980)

Synonym

Brevibacterium luteum

Information on morphological and physiological properties Morphology

[Ref.: #18757]	Colony color	Ivory
[Ref.: #18757]	Incubation period	10-14 days
[Ref.: #18757]	Cultivation medium used	ISP 4

[Ref.: #18757]	Colony color	Honey yellow
[Ref.: #18757]	Incubation period	10-14 days
[Ref.: #18757]	Cultivation medium used	ISP 6

[Ref.: #18757]	Incubation period	10-14 days
[Ref.: #18757]	Cultivation medium used	ISP 2

[Ref.: #18757]	Incubation period	10-14 days
[Ref.: #18757]	Cultivation medium used	ISP 3

[Ref.: #18757]	Incubation period	10-14 days
[Ref.: #18757]	Cultivation medium used	ISP 5

[Ref.: #18757]	Incubation period	10-14 days
[Ref.: #18757]	Cultivation medium used	ISP 7

[Ref.: #18757]	Multicellular complex forming ability	no
[Ref.: #18757]	Medium Name (multicellularity)	ISP 2

[Ref.: #18757]	Multicellular complex forming ability	no
[Ref.: #18757]	Medium Name (multicellularity)	ISP 3

[Ref.: #18757]	Multicellular complex forming ability	no
[Ref.: #18757]	Medium Name (multicellularity)	ISP 4

[Ref.: #18757]	Multicellular complex forming ability	no
[Ref.: #18757]	Medium Name (multicellularity)	ISP 5

[Ref.: #18757]	Multicellular complex forming ability	no
[Ref.: #18757]	Medium Name (multicellularity)	ISP 6

[Ref.: #18757]	Multicellular complex forming ability	no
[Ref.: #18757]	Medium Name (multicellularity)	ISP 7

Information on culture and growth conditions Culture and growth conditions

[Ref.: #18757]

Culture medium

ISP 2

[Ref.: #18757]

Culture medium growth

[Ref.: #18757]

Culture medium composition

expand / minimize

[Ref.: #18757]

Culture medium

ISP 3

[Ref.: #18757]

Culture medium growth

[Ref.: #18757]

Culture medium composition

expand / minimize

[Ref.: #18757]

Culture medium

ISP 4

[Ref.: #18757]

Culture medium growth

[Ref.: #18757]

Culture medium composition

expand / minimize

[Ref.: #18757]

Culture medium

ISP 5

[Ref.: #18757]

Culture medium growth

[Ref.: #18757]

Culture medium composition

expand / minimize

[Ref.: #18757]

Culture medium

ISP 6

[Ref.: #18757]

Culture medium growth

[Ref.: #18757]

Culture medium composition

expand / minimize

[Ref.: #18757]

Culture medium

ISP 7

[Ref.: #18757]

Culture medium growth

[Ref.: #18757]

Culture medium composition

expand / minimize

[Ref.: #8771]

Culture medium

CORYNEBACTERIUM AGAR (DSMZ Medium 53)

[Ref.: #8771]

Culture medium growth

[Ref.: #8771]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8771]

Culture medium composition

expand / minimize

[Ref.: #35425]

Culture medium

MEDIUM 3 - Columbia agar

[Ref.: #35425]

Culture medium growth

[Ref.: #35425]

Culture medium composition

expand / minimize

[Ref.: #120701]

Culture medium

CIP Medium 3

[Ref.: #120701]

Culture medium growth

[Ref.: #120701]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #8771]	growth	30 °C
[Ref.: #18757]	optimum	28 °C
[Ref.: #35425]	growth	30 °C
[Ref.: #47859]	growth	30 °C
[Ref.: #67770]	growth	30 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #47859]

Oxygen tolerance

aerobe

[Ref.: #69481]

Ability of spore formation

no Confidence in %92

[Ref.: #8771]	Name of produced compound	restriction endonuclease BluI
[Ref.: #20216]	Name of produced compound	Restrictase Blu I
[Ref.: #20216]	Name of produced compound	Restrictase Blu II

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #18757]		NaCl		maximum	5	%

[Ref.: #8771]	Murein short key	B05
[Ref.: #8771]	Murein types	B2ß {Gly} [L-Hsr] D-Glu-D-Orn

[Ref.: #67770]

Observation

quinones: MK-9

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #18757]	arabinose ChEBI	+
[Ref.: #68368]	arginine ChEBI	-	hydrolysis	* from API 20E
[Ref.: #18757]	cellulose ChEBI	-
[Ref.: #68368]	citrate ChEBI	-	assimilation	* from API 20E
[Ref.: #68379]	D-glucose ChEBI	+	fermentation	* from API Coryne
[Ref.: #68379]	D-mannitol ChEBI	+	fermentation	* from API Coryne
[Ref.: #68379]	D-ribose ChEBI	+	fermentation	* from API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	esculin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #18757]	fructose ChEBI	-
[Ref.: #68379]	gelatin ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #68368]	gelatin ChEBI	-	hydrolysis	* from API 20E
[Ref.: #18757]	glucose ChEBI	+
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	lactose ChEBI	+	fermentation	* from API Coryne
[Ref.: #68368]	lysine ChEBI	-	degradation	* from API 20E
[Ref.: #68379]	maltose ChEBI	+	fermentation	* from API Coryne
[Ref.: #18757]	mannitol ChEBI	-
[Ref.: #18757]	myo-inositol ChEBI	+
[Ref.: #68379]	nitrate ChEBI	+	reduction	* from API Coryne
[Ref.: #68368]	ornithine ChEBI	-	degradation	* from API 20E
[Ref.: #18757]	raffinose ChEBI	+
[Ref.: #18757]	rhamnose ChEBI	-
[Ref.: #18757]	sucrose ChEBI	+
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68368]	tryptophan ChEBI	-	energy source	* from API 20E
[Ref.: #68379]	urea ChEBI	+	hydrolysis	* from API Coryne
[Ref.: #68368]	urea ChEBI	-	hydrolysis	* from API 20E
[Ref.: #18757]	xylose ChEBI	+
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #68368]		hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68368]		indole ChEBI	no	* from API 20E

	Physiological tests	Metabolite	Indole test
[Ref.: #68368]		indole ChEBI	-	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym, API Coryne
[Ref.: #68382]	alpha-chymotrypsin	+	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym, API Coryne
[Ref.: #68382]	alpha-mannosidase	+	3.2.1.24	* from API zym
[Ref.: #68368]	arginine dihydrolase	-	3.5.3.6	* from API 20E
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym, API Coryne, API 20E
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym, API Coryne
[Ref.: #68379]	beta-glucuronidase	+	3.2.1.31	* from API Coryne
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #68382]	cystine arylamidase	+	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68379]	gelatinase	+		* from API Coryne
[Ref.: #68368]	gelatinase	-		* from API 20E
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68368]	lysine decarboxylase	-	4.1.1.18	* from API 20E
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym, API Coryne
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #68368]	ornithine decarboxylase	-	4.1.1.17	* from API 20E
[Ref.: #68379]	pyrazinamidase	+	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	+	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #68379]	urease	+	3.5.1.5	* from API Coryne
[Ref.: #68368]	urease	-	3.5.1.5	* from API 20E
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #18757]	236	+	-	-	-	-	-	-	-	-	not determinedn.d.	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #18757]	195	+	+	+	-	+	+	+	+	+	+	+	not determinedn.d.	+	+	-	+	+	+	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #18757]	324	not determinedn.d.	-	+	+	-	+	+	+	+	+	+	+	+	+	-	+	+	+	+	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #8771]	Sample type/isolated from	paddy

[Ref.: #47859]	Sample type/isolated from	Paddy

[Ref.: #67770]	Sample type/isolated from	Chinese paddy

[Ref.: #120701]	Sample type/isolated from	Environment, Paddy
* marker position based on {}

Isolation sources categories

#Engineered	#Agriculture	#Field
#Host	#Plants	#Herbaceous plants (Grass,Crops)

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #18757]	Biosafety level	1	Hazard group
[Ref.: #8771]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #120701]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #8771]	C.luteum (DSM20542) 16S rRNA gene	X77437	1472	GenBank ENA	33881 tax ID
[Ref.: #20218]	Curtobacterium luteum 16S-23S ribosomal RNA intergenic spacer region, partial sequence	AY191508	553	GenBank ENA	33881 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Curtobacterium luteum ATCC 15830	GCA_013359885	contig	GenBank ENA	33881 tax ID
[Ref.: #66792]	Curtobacterium luteum DSM 20542	GCA_016907595	contig	GenBank ENA	33881 tax ID	*
[Ref.: #66792]	Curtobacterium luteum JCM 1480	GCA_014646995	scaffold	GenBank ENA	33881 tax ID	*
[Ref.: #66792]	Curtobacterium luteum strain ATCC 15830	33881.5	wgs	PATRIC	33881 tax ID	*
[Ref.: #66792]	Curtobacterium luteum strain DSM 20542	33881.7	wgs	PATRIC	33881 tax ID	*
[Ref.: #66792]	Curtobacterium luteum strain JCM 1480	33881.6	wgs	PATRIC	33881 tax ID	*

[Ref.: #8771]	GC-content	69.8 mol%
[Ref.: #67770]	GC-content	69.8 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #67770]	GC-content	74.9 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Curtobacterium luteum strain ATCC 15830 PATRIC: 33881.3
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	92	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Curtobacterium luteum JCM 1480 NCBI: GCA_014646995.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	87.435	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	98.666	yes
[Ref.: #69480]	aerobic	aerobicⓘ	yes	85.011	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	71.6	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	97	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	78.5	no

Availability in culture collections External links

[Ref.: #8771]

Culture collection no.

DSM 20542, ATCC 15830, CCM 2298, NCIB 11029, CCUG 23848, IAM 1632, JCM 1480, BCRC 12127, CGMCC 1.1913, CIP 102694, IAM 15150, IAM 1623, IFO 12676, IMET 10360, KCCM 12167, KCTC 3493, LMG 8787, NBRC 12676, NCCB 71046, NCIMB 11029, VKM Ac-2188, VKM B-1210

[Ref.: #76765]

SI-ID 264341

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Curtobacterium ginsengisoli sp. nov., isolated from soil of a ginseng field.	Kim MK, Kim YJ, Kim HB, Kim SY, Yi TH, Yang DC	Int J Syst Evol Microbiol	10.1099/ijs.0.65591-0	2008	*
Phylogeny	Nucleic acid hybridization studies on Microbacterium, Curtobacterium, Agromyces and related taxa.	Dopfer H, Stackebrandt E, Fiedler F	J Gen Microbiol	10.1099/00221287-128-8-1697	1982	*

References

#8771

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20542

#18757

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20216

Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI) Curators of the HKI: Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI) .

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#35425 ; Curators of the CIP;
#47859 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 23848

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68368

Automatically annotated from API 20E .

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#76765

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID264341.1 )

#120701 Collection of Institut Pasteur ; Curators of the CIP; CIP 102694
* These data were automatically processed and therefore are not curated

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Search for species Curtobacterium luteum in external resources:
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Curtobacterium luteum strain ATCC 15830

Curtobacterium luteum JCM 1480

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