Agreia pratensis P229/10 is an obligate aerobe, Gram-positive, rod-shaped bacterium that was isolated from phyllosphere of grasses.
Gram-positive rod-shaped obligate aerobe genome sequence 16S sequence Bacteria| @ref 20215 |
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| Domain Bacteria |
| Phylum Actinomycetota |
| Class Actinomycetes |
| Order Micrococcales |
| Family Microbacteriaceae |
| Genus Agreia |
| Species Agreia pratensis |
| Full scientific name Agreia pratensis (Behrendt et al. 2002) Schumann et al. 2003 |
| Synonyms (1) |
| @ref | Name | Growth | Medium link | Composition | |
|---|---|---|---|---|---|
| 5269 | TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) | Medium recipe at MediaDive | Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water | ||
| 18512 | ISP 2 | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |||
| 18512 | ISP 3 | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |||
| 18512 | ISP 4 | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |||
| 18512 | ISP 5 | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |||
| 18512 | ISP 6 | Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes | |||
| 18512 | ISP 7 | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes | |||
| 39984 | MEDIUM 112 - for Janibacter | Distilled water make up to (1000.000 ml);Magnesium sulphate heptahydrate (1.000 g);Agar (20.000 g);Yeast extract (5.000 g);Peptone (10.000 g);Tween 80 (50.000 mg);Glycerol (2.000 g);Malt extract (5.000 g);Beef extract (2.000 g);Casamino acids (5.000 g);Ca | |||
| 121950 | CIP Medium 112 | Medium recipe at CIP |
| 121950 | Oxygen toleranceobligate aerobe |
| 67770 | Observationquinones: MK-10, MK-11 |
| @ref | Chebi-ID | Metabolite | Utilization activity | Kind of utilization tested | |
|---|---|---|---|---|---|
| 18512 | 22599 ChEBI | arabinose | + | ||
| 18512 | 62968 ChEBI | cellulose | + | ||
| 121950 | 16947 ChEBI | citrate | - | carbon source | |
| 68379 | 17634 ChEBI | D-glucose | - | fermentation | from API Coryne |
| 68379 | 16899 ChEBI | D-mannitol | - | fermentation | from API Coryne |
| 68379 | 16988 ChEBI | D-ribose | - | fermentation | from API Coryne |
| 68379 | 65327 ChEBI | D-xylose | - | fermentation | from API Coryne |
| 68379 | 4853 ChEBI | esculin | + | hydrolysis | from API Coryne |
| 121950 | 4853 ChEBI | esculin | + | hydrolysis | |
| 18512 | 28757 ChEBI | fructose | + | ||
| 68379 | 5291 ChEBI | gelatin | - | hydrolysis | from API Coryne |
| 18512 | 17234 ChEBI | glucose | - | ||
| 68379 | 28087 ChEBI | glycogen | - | fermentation | from API Coryne |
| 121950 | 606565 ChEBI | hippurate | + | hydrolysis | |
| 68379 | 17716 ChEBI | lactose | - | fermentation | from API Coryne |
| 68379 | 17306 ChEBI | maltose | - | fermentation | from API Coryne |
| 18512 | 29864 ChEBI | mannitol | + | ||
| 18512 | 17268 ChEBI | myo-inositol | - | ||
| 68379 | 17632 ChEBI | nitrate | - | reduction | from API Coryne |
| 121950 | 17632 ChEBI | nitrate | - | reduction | |
| 121950 | 17632 ChEBI | nitrate | - | respiration | |
| 121950 | 16301 ChEBI | nitrite | - | reduction | |
| 18512 | 16634 ChEBI | raffinose | - | ||
| 18512 | 26546 ChEBI | rhamnose | - | ||
| 18512 | 17992 ChEBI | sucrose | + | ||
| 68379 | 17992 ChEBI | sucrose | - | fermentation | from API Coryne |
| 68379 | 16199 ChEBI | urea | + | hydrolysis | from API Coryne |
| 18512 | 18222 ChEBI | xylose | - |
| @ref | Value | Activity | Ec | |
|---|---|---|---|---|
| 68382 | acid phosphatase | + | 3.1.3.2 | from API zym |
| 121950 | alcohol dehydrogenase | - | 1.1.1.1 | |
| 68382 | alkaline phosphatase | + | 3.1.3.1 | from API zym |
| 68379 | alkaline phosphatase | + | 3.1.3.1 | from API Coryne |
| 68382 | alpha-chymotrypsin | - | 3.4.21.1 | from API zym |
| 68382 | alpha-fucosidase | - | 3.2.1.51 | from API zym |
| 68382 | alpha-galactosidase | + | 3.2.1.22 | from API zym |
| 68382 | alpha-glucosidase | + | 3.2.1.20 | from API zym |
| 68379 | alpha-glucosidase | - | 3.2.1.20 | from API Coryne |
| 68382 | alpha-mannosidase | + | 3.2.1.24 | from API zym |
| 121950 | amylase | - | ||
| 68382 | beta-galactosidase | + | 3.2.1.23 | from API zym |
| 121950 | beta-galactosidase | + | 3.2.1.23 | |
| 68379 | beta-galactosidase | - | 3.2.1.23 | from API Coryne |
| 68382 | beta-glucosidase | + | 3.2.1.21 | from API zym |
| 68379 | beta-glucosidase | + | 3.2.1.21 | from API Coryne |
| 68382 | beta-glucuronidase | - | 3.2.1.31 | from API zym |
| 68379 | beta-glucuronidase | - | 3.2.1.31 | from API Coryne |
| 121950 | caseinase | - | 3.4.21.50 | |
| 121950 | catalase | + | 1.11.1.6 | |
| 68382 | cystine arylamidase | + | 3.4.11.3 | from API zym |
| 121950 | DNase | - | ||
| 68382 | esterase lipase (C 8) | + | from API zym | |
| 121950 | gamma-glutamyltransferase | + | 2.3.2.2 | |
| 121950 | gelatinase | - | ||
| 68379 | gelatinase | - | from API Coryne | |
| 121950 | lecithinase | - | ||
| 68382 | leucine arylamidase | + | 3.4.11.1 | from API zym |
| 121950 | lipase | - | ||
| 68382 | lipase (C 14) | - | from API zym | |
| 121950 | lysine decarboxylase | - | 4.1.1.18 | |
| 68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 | from API zym |
| 68379 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 | from API Coryne |
| 121950 | ornithine decarboxylase | - | 4.1.1.17 | |
| 121950 | oxidase | - | ||
| 121950 | phenylalanine ammonia-lyase | + | 4.3.1.24 | |
| 68379 | pyrazinamidase | - | 3.5.1.B15 | from API Coryne |
| 68379 | pyrrolidonyl arylamidase | - | 3.4.19.3 | from API Coryne |
| 121950 | tryptophan deaminase | - | ||
| 121950 | tween esterase | + | ||
| 121950 | urease | - | 3.5.1.5 | |
| 68379 | urease | + | 3.5.1.5 | from API Coryne |
| 68382 | valine arylamidase | + | from API zym |
| Cat1 | Cat2 | Cat3 | |
|---|---|---|---|
| #Host | #Plants | #Herbaceous plants (Grass,Crops) | |
| #Host Body-Site | #Plant | #Leaf (Phyllosphere) |
Global distribution of 16S sequence AJ310412 (>99% sequence identity) for Agreia from Microbeatlas ![]()
| @ref | Description | Assembly level | INSDC accession | BV-BRC accession | IMG accession | NCBI tax ID | Score | |
|---|---|---|---|---|---|---|---|---|
| 67770 | IMG-taxon 2708742395 annotated assembly for Agreia pratensis VKM Ac-2510 | contig | 150121 | 76.53 |
| @ref | Description | Accession | Length | Database | NCBI tax ID | |
|---|---|---|---|---|---|---|
| 5269 | Subtercola pratensis partial 16S rRNA gene, strain DSM 14226T | AJ310412 | 1513 | 150121 |
| @ref | GC-content (mol%) | Method | |
|---|---|---|---|
| 5269 | 65 | high performance liquid chromatography (HPLC) |
| @ref | Trait | Model | Prediction | Confidence in % | In training data |
|---|---|---|---|---|---|
| 125438 | gram-positive | gram-positiveⓘ | yes | 89.71 | no |
| 125438 | anaerobic | anaerobicⓘ | no | 96.96 | no |
| 125438 | aerobic | aerobicⓘ | yes | 87.33 | yes |
| 125438 | spore-forming | spore-formingⓘ | no | 75.09 | no |
| 125438 | thermophilic | thermophileⓘ | no | 97.43 | yes |
| 125438 | flagellated | motile2+ⓘ | no | 91.00 | no |
| Topic | Title | Authors | Journal | DOI | Year | |
|---|---|---|---|---|---|---|
| Monitoring of soil microbial inoculants and their impact on maize (Zea mays L.) rhizosphere using T-RFLP molecular fingerprint method | Kari A, Nagymate Z, Romsics C, Vajna B, Kutasi J, Puspan I, Karpati E, Kovacs R, Marialigeti K. | Appl Soil Ecol | 10.1016/j.apsoil.2019.03.010 | 2019 | ||
| Phylogeny | Reclassification of Subtercola pratensis Behrendt et al. 2002 as Agreia pratensis comb. nov. | Schumann P, Behrendt U, Ulrich A, Suzuki K. | Int J Syst Evol Microbiol | 10.1099/ijs.0.02664-0 | 2003 | |
| Genetics | Systematic Affiliation and Genome Analysis of Subtercola vilae DB165T with Particular Emphasis on Cold Adaptation of an Isolate from a High-Altitude Cold Volcano Lake. | Villalobos AS, Wiese J, Imhoff JF, Dorador C, Keller A, Hentschel U. | Microorganisms | 10.3390/microorganisms7040107 | 2019 | |
| Efficacy of pH elevation as a bactericidal strategy for treating ballast water of freight carriers. | Starliper CE, Watten BJ, Iwanowicz DD, Green PA, Bassett NL, Adams CR. | J Adv Res | 10.1016/j.jare.2015.02.005 | 2015 | ||
| Subtercola endophyticus sp. nov., a cold-adapted bacterium isolated from Abies koreana. | Jiang L, Peng Y, Seo J, Jeon D, Jo MG, Lee JH, Jeong JC, Kim CY, Park HC, Lee J. | Sci Rep | 10.1038/s41598-022-16116-3 | 2022 | ||
| Subtercola vilae sp. nov., a novel actinobacterium from an extremely high-altitude cold volcano lake in Chile. | Villalobos AS, Wiese J, Aguilar P, Dorador C, Imhoff JF. | Antonie Van Leeuwenhoek | 10.1007/s10482-017-0994-4 | 2018 | ||
| Phylogeny | Diversity of grass-associated Microbacteriaceae isolated from the phyllosphere and litter layer after mulching the sward; polyphasic characterization of Subtercola pratensis sp. nov., Curtobacterium herbarum sp. nov. and Plantibacter flavus gen. nov., sp. nov. | Behrendt U, Ulrich A, Schumann P, Naumann D, Suzuki KI | Int J Syst Evol Microbiol | 10.1099/00207713-52-5-1441 | 2002 |
| #5269 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 14246 |
| #18512 | Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig . |
| #20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 ) |
| #39984 | ; Curators of the CIP; |
| #67770 | Japan Collection of Microorganism (JCM) ; Curators of the JCM; |
| #68379 | Automatically annotated from API Coryne . |
| #68382 | Automatically annotated from API zym . |
| #69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta . |
| #121950 | Collection of Institut Pasteur ; Curators of the CIP; CIP 108226 |
| #125438 | Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 ) |
| #126262 | A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 ) |
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