Methanopyrus kandleri (DSM 6324, JCM 9639, AV19)

Name: Methanopyrus kandleri (DSM 6324, JCM 9639, AV19)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 7044

Type strain

Strain Designation AV19

Culture col. no. DSM 6324 JCM 9639 AV19 NBRC 100938

NCBI tax ID(s) 2320

Special Collections DSMZ JCM

History <- K. O. Stetter, Univ. Regensburg, Germany; AV19 <- R. Huber and K. O. Stetter {1988} DSM 6324 <-- K. O. Stetter and R. Huber AV19.

Links

Methanopyrus kandleri AV19 is an anaerobe, hyperthermophilic prokaryote that was isolated from geothermally heated deep sea sediment.

anaerobe hyperthermophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 48150

DSM 6324,JCM 9639,NBRC 100938

@ref 20215

Last LPSN update 2026-02-09 11:19:22

Domain Archaea

Phylum Methanobacteriota

Class Methanopyri

Order Methanopyrales

Family Methanopyraceae

Genus Methanopyrus

Species Methanopyrus kandleri

Full scientific name Methanopyrus kandleri Kurr et al. 1992

BacDive ID	Other strains from **Methanopyrus kandleri** (1)	Type strain
161561	M. kandleri JCM 15049

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
2591	METHANOPYRUS MEDIUM (DSMZ Medium 511)		Medium recipe at MediaDive	Name: METHANOPYRUS MEDIUM (DSMZ Medium 511) Composition: MgCl2 x 6 H2O 4.46872 g/l NaHCO3 0.993049 g/l Na2SO4 0.804369 g/l Na2S x 9 H2O 0.496524 g/l KCl 0.297915 g/l NH4Cl 0.248262 g/l KH2PO4 0.0893744 g/l K2HPO4 x 3 H2O 0.0695134 g/l NaBr 0.0397219 g/l MgSO4 x 7 H2O 0.0297915 g/l SrCl2 x 6 H2O 0.0178749 g/l Nitrilotriacetic acid 0.0148957 g/l H3BO3 0.0129096 g/l NaCl 0.00993049 g/l MnSO4 x H2O 0.00496524 g/l Na2WO4 x 2 H2O 0.0019861 g/l Fe(NH4)2(SO4)2 x 6 H2O 0.0019861 g/l (NH4)2Ni(SO4)2 0.0019861 g/l CoSO4 x 7 H2O 0.00178749 g/l ZnSO4 x 7 H2O 0.00178749 g/l FeSO4 x 7 H2O 0.000993049 g/l CaCl2 x 2 H2O 0.000993049 g/l Na-silicate 0.000993049 g/l NaF 0.000595829 g/l Sodium resazurin 0.000496524 g/l KNO3 0.000397219 g/l NiCl2 x 6 H2O 0.000297915 g/l AlK(SO4)2 x 12 H2O 0.00019861 g/l Pyridoxine hydrochloride 9.93049e-05 g/l CuSO4 x 5 H2O 9.93049e-05 g/l Na2MoO4 x 2 H2O 9.93049e-05 g/l Calcium D-(+)-pantothenate 4.96524e-05 g/l Riboflavin 4.96524e-05 g/l Nicotinic acid 4.96524e-05 g/l Thiamine HCl 4.96524e-05 g/l (DL)-alpha-Lipoic acid 4.96524e-05 g/l p-Aminobenzoic acid 4.96524e-05 g/l Biotin 1.9861e-05 g/l Folic acid 1.9861e-05 g/l KI 1.24131e-05 g/l Na2SeO3 x 5 H2O 2.97915e-06 g/l Na2HPO4 x 3 H2O 2.48262e-06 g/l Vitamin B12 9.93049e-07 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
2591	positive	growth	98	hyperthermophilic
67770	positive	growth	95	hyperthermophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
2591	anaerobe
125438	anaerobe	90.728
125439	facultative anaerobe	95.3

Spore formation

@ref	Spore formation	Confidence
125439		94

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Marine
#Environmental	#Aquatic	#Sediment

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
2591	geothermally heated deep sea sediment	Gulf of California, Guaymas Basin	Mexico	MEX	North America
67770	Hydrothermally heated deep sea sediment at the Guaymas Basin, the Gulf of California

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
2591	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM718v1 assembly for Methanopyrus kandleri AV19	complete	GCA_000007185	190192.8	638154507	190192	98.08

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
67770	Methanopyrus kandleri ribosomal RNA sequence	M59932	1477		2320

GC content

@ref	GC-content (mol%)	Method
2591	60
67770	60	high performance liquid chromatography (HPLC)
67770	59	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Methanopyrus kandleri AV19
IMG: 638154507

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	94.00	no
125439	motility	BacteriaNetⓘ	yes	67.00	no
125439	gram_stain	BacteriaNetⓘ	positive	73.20	no
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	95.30	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Methanopyrus kandleri AV19 AV19
NCBI: GCA_000007185.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	60.56	no
125438	anaerobic	anaerobicⓘ	yes	90.73	no
125438	aerobic	aerobicⓘ	no	91.25	yes
125438	spore-forming	spore-formingⓘ	no	86.46	no
125438	thermophilic	thermophileⓘ	yes	71.03	yes
125438	flagellated	motile2+ⓘ	no	73.98	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Data on the optimization of an archaea-specific probe-based qPCR assay.	Bomberg M, Miettinen H.	Data Brief	10.1016/j.dib.2020.106610	2020
	Large-Scale Molecular Evolutionary Analysis Uncovers a Variety of Polynucleotide Kinase Clp1 Family Proteins in the Three Domains of Life.	Saito M, Sato A, Nagata S, Tamaki S, Tomita M, Suzuki H, Kanai A.	Genome Biol Evol	10.1093/gbe/evz195	2019
Genetics	The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.	Slesarev AI, Mezhevaya KV, Makarova KS, Polushin NN, Shcherbinina OV, Shakhova VV, Belova GI, Aravind L, Natale DA, Rogozin IB, Tatusov RL, Wolf YI, Stetter KO, Malykh AG, Koonin EV, Kozyavkin SA.	Proc Natl Acad Sci U S A	10.1073/pnas.032671499	2002
	Organic solutes in hyperthermophilic archaea.	Martins LO, Huber R, Huber H, Stetter KO, Da Costa MS, Santos H.	Appl Environ Microbiol	10.1128/aem.63.3.896-902.1997	1997
Metabolism	Helix-hairpin-helix protein MJ1434 from Methanocaldococcus jannaschii and EndoIV homologue TTC0482 from Thermus thermophilus HB27 do not process DNA uracil residues.	Schomacher L, Smolorz S, Ciirdaeva E, Ber S, Kramer W, Fritz HJ.	Nucleic Acids Res	10.1093/nar/gkq270	2010
Metabolism	Presence of acetyl coenzyme A (CoA) carboxylase and propionyl-CoA carboxylase in autotrophic Crenarchaeota and indication for operation of a 3-hydroxypropionate cycle in autotrophic carbon fixation.	Menendez C, Bauer Z, Huber H, Gad'on N, Stetter KO, Fuchs G.	J Bacteriol	10.1128/jb.181.4.1088-1098.1999	1999
Enzymology	ADP-dependent phosphofructokinases in mesophilic and thermophilic methanogenic archaea.	Verhees CH, Tuininga JE, Kengen SW, Stams AJ, van der Oost J, de Vos WM.	J Bacteriol	10.1128/jb.183.24.7145-7153.2001	2001
Phylogeny	Discontinuous occurrence of the hsp70 (dnaK) gene among Archaea and sequence features of HSP70 suggest a novel outlook on phylogenies inferred from this protein.	Gribaldo S, Lumia V, Creti R, Conway de Macario E, Sanangelantoni A, Cammarano P.	J Bacteriol	10.1128/jb.181.2.434-443.1999	1999
	Compilation of small ribosomal subunit RNA structures.	Neefs JM, Van de Peer Y, De Rijk P, Chapelle S, De Wachter R.	Nucleic Acids Res	10.1093/nar/21.13.3025	1993

References

#2591	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 6324
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Methanopyrus kandleri (DSM 6324, JCM 9639, AV19)

Name and taxonomic classification

Morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000007185

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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