Archive BacDiveID:10.13145/bacdive7044.20230509.8.1

Strain identifier

BacDive ID: 7044

Type strain:

Species: Methanopyrus kandleri

Strain Designation: AV19

Strain history: DSM 6324 <-- K. O. Stetter and R. Huber AV19.

NCBI tax ID(s): 2320 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 2591

BacDive-ID: 7044

DSM-Number: 6324

keywords: genome sequence, 16S sequence, Archaea, anaerobe, hyperthermophilic

description: Methanopyrus kandleri AV19 is an anaerobe, hyperthermophilic archaeon that was isolated from geothermally heated deep sea sediment.

NCBI tax id

NCBI tax id: 2320
Matching level: species

strain history

@ref	history
2591	<- K. O. Stetter, Univ. Regensburg, Germany; AV19 <- R. Huber and K. O. Stetter {1988}
67770	DSM 6324 <-- K. O. Stetter and R. Huber AV19.

doi: 10.13145/bacdive7044.20230509.8.1

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/archaea
keyword: phylum/methanobacteriota
domain: Archaea
phylum: Methanobacteriota
class: Methanopyri
order: Methanopyrales
family: Methanopyraceae
genus: Methanopyrus
species: Methanopyrus kandleri
full scientific name: Methanopyrus kandleri Kurr et al. 1992

@ref: 2591

domain: Archaea

phylum: Euryarchaeota

class: Methanopyri

order: Methanopyrales

family: Methanopyraceae

genus: Methanopyrus

species: Methanopyrus kandleri

full scientific name: Methanopyrus kandleri Kurr et al. 1992

strain designation: AV19

type strain: yes

Culture and growth conditions

culture medium

@ref: 2591
name: METHANOPYRUS MEDIUM (DSMZ Medium 511)
growth: yes
link: https://mediadive.dsmz.de/medium/511
composition: Name: METHANOPYRUS MEDIUM (DSMZ Medium 511) Composition: MgCl2 x 6 H2O 4.46872 g/l NaHCO3 0.993049 g/l Na2SO4 0.804369 g/l Na2S x 9 H2O 0.496524 g/l KCl 0.297915 g/l NH4Cl 0.248262 g/l KH2PO4 0.0893744 g/l K2HPO4 x 3 H2O 0.0695134 g/l NaBr 0.0397219 g/l MgSO4 x 7 H2O 0.0297915 g/l SrCl2 x 6 H2O 0.0178749 g/l Nitrilotriacetic acid 0.0148957 g/l H3BO3 0.0129096 g/l NaCl 0.00993049 g/l MnSO4 x H2O 0.00496524 g/l Na2WO4 x 2 H2O 0.0019861 g/l Fe(NH4)2(SO4)2 x 6 H2O 0.0019861 g/l (NH4)2Ni(SO4)2 0.0019861 g/l CoSO4 x 7 H2O 0.00178749 g/l ZnSO4 x 7 H2O 0.00178749 g/l FeSO4 x 7 H2O 0.000993049 g/l CaCl2 x 2 H2O 0.000993049 g/l Na-silicate 0.000993049 g/l NaF 0.000595829 g/l Sodium resazurin 0.000496524 g/l KNO3 0.000397219 g/l NiCl2 x 6 H2O 0.000297915 g/l AlK(SO4)2 x 12 H2O 0.00019861 g/l Pyridoxine hydrochloride 9.93049e-05 g/l CuSO4 x 5 H2O 9.93049e-05 g/l Na2MoO4 x 2 H2O 9.93049e-05 g/l Calcium D-(+)-pantothenate 4.96524e-05 g/l Riboflavin 4.96524e-05 g/l Nicotinic acid 4.96524e-05 g/l Thiamine HCl 4.96524e-05 g/l (DL)-alpha-Lipoic acid 4.96524e-05 g/l p-Aminobenzoic acid 4.96524e-05 g/l Biotin 1.9861e-05 g/l Folic acid 1.9861e-05 g/l KI 1.24131e-05 g/l Na2SeO3 x 5 H2O 2.97915e-06 g/l Na2HPO4 x 3 H2O 2.48262e-06 g/l Vitamin B12 9.93049e-07 g/l Distilled water

culture temp

@ref	growth	type	temperature	range
2591	positive	growth	98	hyperthermophilic
67770	positive	growth	95	hyperthermophilic

Physiology and metabolism

oxygen tolerance

@ref: 2591
oxygen tolerance: anaerobe

Isolation, sampling and environmental information

isolation

@ref	sample type	geographic location	country	origin.country	continent
2591	geothermally heated deep sea sediment	Gulf of California, Guaymas Basin	Mexico	MEX	North America
67770	Hydrothermally heated deep sea sediment at the Guaymas Basin, the Gulf of California

isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Marine
#Environmental	#Aquatic	#Sediment

Safety information

risk assessment

@ref: 2591
biosafety level: 1
biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@ref: 67770
description: Methanopyrus kandleri ribosomal RNA sequence
accession: M59932
length: 1477
database: ena
NCBI tax ID: 2320

Genome sequences

@ref	description	accession	assembly level	database	NCBI tax ID
66792	Methanopyrus kandleri AV19	GCA_000007185	complete	ncbi	190192
66792	Methanopyrus kandleri AV19	638154507	complete	img	190192
66792	Methanopyrus kandleri AV19	190192.8	complete	patric	190192

GC content

@ref	GC-content	method
2591	60
67770	60	high performance liquid chromatography (HPLC)
67770	59	thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

trait	prediction	confidence	training_data
motile	no	83.967	no
flagellated	no	93.865	no
gram-positive	no	95.419	no
anaerobic	yes	99.163	yes
halophile	no	77.63	no
spore-forming	no	95.176	no
glucose-util	yes	76.263	no
aerobic	no	97.487	yes
thermophile	yes	99.304	yes
glucose-ferment	no	56.235	no

External links

@ref: 2591

culture collection no.: DSM 6324, JCM 9639, NBRC 100938

straininfo link

@ref: 76503
straininfo: 48150

Reference

@id	authors	catalogue	doi/url	title
2591	Curators of the DSMZ	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 6324)	https://www.dsmz.de/collection/catalogue/details/culture/DSM-6324
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.		10.1099/ijsem.0.004332	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770	Curators of the JCM		https://jcm.brc.riken.jp/en/
76503	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.		10.60712/SI-ID48150.1	StrainInfo: A central database for resolving microbial strain identifiers