Methanococcus voltae (DSM 1537, ATCC 33273, OCM 70)

Name: Methanococcus voltae (DSM 1537, ATCC 33273, OCM 70)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 6994

Type strain

Strain Designation PS

Culture col. no. DSM 1537 ATCC 33273 OCM 70 PS

NCBI tax ID(s) 523842 2188

Special Collections DSMZ

History DSMZ <- W. E. Balch, University of Illinois, Urbana, USA; PS <- J. M. Ward, University of Miami, Florida, USA; (Methanococcus sp.) {1970}

Links

Methanococcus voltae PS is an anaerobe archaeon that was isolated from mud.

anaerobe genome sequence 16S sequence Archaea

Name and taxonomic classification

SI-ID 47450

DSM 1537,ATCC 33273,NBRC 100457,NCAIM B.01912

@ref 20215

Last LPSN update 2026-05-29 08:26:32

Domain Archaea

Phylum Methanobacteriota

Class Methanococci

Order Methanococcales

Family Methanococcaceae

Genus Methanococcus

Species Methanococcus voltae

Full scientific name Methanococcus voltae Balch and Wolfe 1981

BacDive ID	Other strains from **Methanococcus voltae** (6)	Type strain
6995	M. voltae PS-3, DSM 4254
6996	M. voltae PS-6, DSM 4310
6997	M. voltae PS-7, DSM 4311
6998	M. voltae DSM 7078, MCM B603
6999	M. voltae P2F9701a, DSM 14649, OCM 745
161888	M. voltae JCM 16865, OCM 197

Morphology

Cell morphology

@ref	Gram stain	Confidence
125439	negative	98.034

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
814	METHANOGENIUM MEDIUM (H2/CO2) (DSMZ Medium 141)		Medium recipe at MediaDive	Name: METHANOGENIUM MEDIUM (H2/CO2) (DSMZ Medium 141) Composition: NaHCO3 4.89237 g/l MgCl2 x 6 H2O 3.91389 g/l Yeast extract 1.95695 g/l Trypticase peptone 1.95695 g/l Na-acetate 0.978474 g/l Na2S x 9 H2O 0.489237 g/l L-Cysteine HCl x H2O 0.489237 g/l KCl 0.332681 g/l NH4Cl 0.244618 g/l K2HPO4 0.136986 g/l MgSO4 x 7 H2O 0.0293542 g/l Nitrilotriacetic acid 0.0146771 g/l NaCl 0.00978474 g/l MnSO4 x H2O 0.00489237 g/l Fe(NH4)2(SO4)2 x 6 H2O 0.00195695 g/l CoSO4 x 7 H2O 0.00176125 g/l ZnSO4 x 7 H2O 0.00176125 g/l FeSO4 x 7 H2O 0.000978474 g/l CaCl2 x 2 H2O 0.000978474 g/l Sodium resazurin 0.000489237 g/l NiCl2 x 6 H2O 0.000293542 g/l AlK(SO4)2 x 12 H2O 0.000195695 g/l CuSO4 x 5 H2O 9.78474e-05 g/l Pyridoxine hydrochloride 9.78474e-05 g/l Na2MoO4 x 2 H2O 9.78474e-05 g/l H3BO3 9.78474e-05 g/l Nicotinic acid 4.89237e-05 g/l Riboflavin 4.89237e-05 g/l (DL)-alpha-Lipoic acid 4.89237e-05 g/l Thiamine HCl 4.89237e-05 g/l Calcium D-(+)-pantothenate 4.89237e-05 g/l p-Aminobenzoic acid 4.89237e-05 g/l Folic acid 1.95695e-05 g/l Biotin 1.95695e-05 g/l Na2WO4 x 2 H2O 3.91389e-06 g/l Na2SeO3 x 5 H2O 2.93542e-06 g/l Vitamin B12 9.78474e-07 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
814	positive	growth	35

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
814	anaerobe
125439	obligate aerobe	97.319

Spore formation

@ref	Spore formation	Confidence
125439		97.389

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Terrestrial	#Mud (Sludge)

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
814	mud	Florida, Wacasassa Estuary	USA	USA	North America

Taxonmaps

69479

Global distribution of 16S sequence KC139248 (>99% sequence identity) for Methanococcus voltae subclade from Microbeatlas

Aquatic Samples	2
Soil Samples	0
Animal Samples	1
Plant Samples	0
Total Samples	3

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
814	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM2480703v1 assembly for Methanococcus voltae PS	contig	GCA_024807035	523842.4	2918187917	523842	76.83

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Methanococcus voltae 16S ribosomal RNA	M59290	1466	2188
20218	Methanococcus voltae PS strain NBRC 100457 16S ribosomal RNA gene, partial sequence	KC139248	1398	523842
814	Methanococcus voltae 16S ribosomal RNA, complete sequence	U38461	1466	523842

GC content

814

GC-content (mol%)30.7

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM2480703v1 assembly for Methanococcus voltae PS
NCBI: GCA_024807035

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	97.32	no
125439	gram_stain	BacteriaNetⓘ	negative	98.03	no
125439	motility	BacteriaNetⓘ	no	69.62	no
125439	spore_formation	BacteriaNetⓘ	no	97.39	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Teaching old dogs new tricks: genetic engineering methanogens.	Myers T, Myers T, Dykstra CM.	Appl Environ Microbiol	10.1128/aem.02247-23	2024
	Drivers of Antibiotic Resistance Gene Abundance in an Urban River.	Morina JC, Franklin RB.	Antibiotics (Basel)	10.3390/antibiotics12081270	2023
	Archaeosomes: New Generation of Liposomes Based on Archaeal Lipids for Drug Delivery and Biomedical Applications.	Santhosh PB, Genova J.	ACS Omega	10.1021/acsomega.2c06034	2023
Metabolism	Hydrogen production by Sulfurospirillum species enables syntrophic interactions of Epsilonproteobacteria.	Kruse S, Goris T, Westermann M, Adrian L, Diekert G.	Nat Commun	10.1038/s41467-018-07342-3	2018
	Identification of the Methanococcus voltae S-layer structural gene.	Konisky J, Lynn D, Hoppert M, Mayer F, Haney P.	J Bacteriol	10.1128/jb.176.6.1790-1792.1994	1994
	Transcriptional regulation of genes encoding the selenium-free [NiFe]-hydrogenases in the archaeon Methanococcus voltae involves positive and negative control elements.	Noll I, Muller S, Klein A.	Genetics	10.1093/genetics/152.4.1335	1999
	Hydroxydiether Lipid Structures in Methanosarcina spp. and Methanococcus voltae.	Sprott GD, Dicaire CJ, Choquet CG, Patel GB, Ekiel I.	Appl Environ Microbiol	10.1128/aem.59.3.912-914.1993	1993
Phylogeny	Estimation of methanogen biomass by quantitation of coenzyme M.	Elias DA, Krumholz LR, Tanner RS, Suflita JM.	Appl Environ Microbiol	10.1128/aem.65.12.5541-5545.1999	1999
	Natural and Electroporation-Mediated Transformation of Methanococcus voltae Protoplasts.	Patel GB, Nash JH, Agnew BJ, Sprott GD.	Appl Environ Microbiol	10.1128/aem.60.3.903-907.1994	1994
Metabolism	Excretion of amino acids by 1,2,4-triazole-3-alanine-resistant mutants of Methanococcus voltae.	Sment KA, Konisky J.	Appl Environ Microbiol	10.1128/aem.55.5.1295-1297.1989	1989
Pathogenicity	Isolation and characterization of a copper-resistant methanogen from a copper-mining soil sample.	Kim BK, Conway de Macario E, Nolling J, Daniels L.	Appl Environ Microbiol	10.1128/aem.62.7.2629-2635.1996	1996
	Fibrillarin-like proteins occur in the domain Archaea.	Amiri KA.	J Bacteriol	10.1128/jb.176.7.2124-2127.1994	1994
Metabolism	Isolation of a coenzyme M-auxotrophic mutant and transformation by electroporation in Methanococcus voltae.	Micheletti PA, Sment KA, Konisky J.	J Bacteriol	10.1128/jb.173.11.3414-3418.1991	1991
	Insertional Mutations in the Hydrogenase vhc and frc Operons Encoding Selenium-Free Hydrogenases in Methanococcus voltae.	Berghofer Y, Klein A.	Appl Environ Microbiol	10.1128/aem.61.5.1770-1775.1995	1995
Metabolism	Transport of coenzyme M (2-mercaptoethanesulfonic acid) and methylcoenzyme M [(2-methylthio)ethanesulfonic acid] in Methanococcus voltae: identification of specific and general uptake systems.	Dybas M, Konisky J.	J Bacteriol	10.1128/jb.171.11.5866-5871.1989	1989
	Termination of a transcription unit comprising highly expressed genes in the archaebacterium Methanococcus voltae.	Muller B, Allmansberger R, Klein A.	Nucleic Acids Res	10.1093/nar/13.18.6439	1985
Metabolism	Characterization of bromoethanesulfonate-resistant mutants of Methanococcus voltae: evidence of a coenzyme M transport system.	Santoro N, Konisky J.	J Bacteriol	10.1128/jb.169.2.660-665.1987	1987
	Light sensitivity of methanogenic archaebacteria.	Olson KD, McMahon CW, Wolfe RS.	Appl Environ Microbiol	10.1128/aem.57.9.2683-2686.1991	1991
Pathogenicity	Analysis of drug resistance in the archaebacterium Methanococcus voltae with respect to potential use in genetic engineering.	Possot O, Gernhardt P, Klein A, Sibold L.	Appl Environ Microbiol	10.1128/aem.54.3.734-740.1988	1988
	Isolation and characterization of the 7S RNA gene from Methanococcus voltae.	Kaine BP, Merkel VL.	J Bacteriol	10.1128/jb.171.8.4261-4266.1989	1989
Enzymology	Characterization of enzymes of the branched-chain amino acid biosynthetic pathway in Methanococcus spp.	Xing RY, Whitman WB.	J Bacteriol	10.1128/jb.173.6.2086-2092.1991	1991
	Immunocytochemical localization of component C of the methylreductase system in Methanococcus voltae and Methanobacterium thermoautotrophicum.	Ossmer R, Mund T, Hartzell PL, Konheiser U, Kohring GW, Klein A, Wolfe RS, Gottschalk G, Mayer F.	Proc Natl Acad Sci U S A	10.1073/pnas.83.16.5789	1986
	Folic acid and pteroylpolyglutamate contents of archaebacteria.	Worrell VE, Nagle DP.	J Bacteriol	10.1128/jb.170.9.4420-4423.1988	1988
	Cloning and physical mapping of RNA polymerase genes from Methanobacterium thermoautotrophicum and comparison of homologies and gene orders with those of RNA polymerase genes from other methanogenic archaebacteria.	Schallenberg J, Moes M, Truss M, Reiser W, Thomm M, Stetter KO, Klein A.	J Bacteriol	10.1128/jb.170.5.2247-2253.1988	1988
	Genetic transformation in the methanogen Methanococcus voltae PS.	Bertani G, Baresi L.	J Bacteriol	10.1128/jb.169.6.2730-2738.1987	1987
Metabolism	Thermal adaptation analyzed by comparison of protein sequences from mesophilic and extremely thermophilic Methanococcus species.	Haney PJ, Badger JH, Buldak GL, Reich CI, Woese CR, Olsen GJ.	Proc Natl Acad Sci U S A	10.1073/pnas.96.7.3578	1999
Metabolism	Hydrogenotrophic methanogenesis by moderately acid-tolerant methanogens of a methane-emitting acidic peat.	Horn MA, Matthies C, Kusel K, Schramm A, Drake HL.	Appl Environ Microbiol	10.1128/aem.69.1.74-83.2003	2003
	Growth and plating efficiency of methanococci on agar media.	Jones WJ, Whitman WB, Fields RD, Wolfe RS.	Appl Environ Microbiol	10.1128/aem.46.1.220-226.1983	1983
	Isolation and characterization of a novel thermophilic, freshwater methanogen.	Harris JE, Pinn PA, Davis RP.	Appl Environ Microbiol	10.1128/aem.48.6.1123-1128.1984	1984
Metabolism	Nutrition and carbon metabolism of Methanococcus voltae.	Whitman WB, Ankwanda E, Wolfe RS.	J Bacteriol	10.1128/jb.149.3.852-863.1982	1982
	Compilation of 5S rRNA and 5S rRNA gene sequences.	Specht T, Wolters J, Erdmann VA.	Nucleic Acids Res	10.1093/nar/18.suppl.2215	1990
Enzymology	Characterization of amino acid aminotransferases of Methanococcus aeolicus.	Xing RY, Whitman WB.	J Bacteriol	10.1128/jb.174.2.541-548.1992	1992
Metabolism	Presence of acetyl coenzyme A (CoA) carboxylase and propionyl-CoA carboxylase in autotrophic Crenarchaeota and indication for operation of a 3-hydroxypropionate cycle in autotrophic carbon fixation.	Menendez C, Bauer Z, Huber H, Gad'on N, Stetter KO, Fuchs G.	J Bacteriol	10.1128/jb.181.4.1088-1098.1999	1999
	Detection and quantitation of methanogens by enzyme-linked immunosorbent assay.	Archer DB.	Appl Environ Microbiol	10.1128/aem.48.4.797-801.1984	1984
Phylogeny	Methanogens: reevaluation of a unique biological group.	Balch WE, Fox GE, Magrum LJ, Woese CR, Wolfe RS.	Microbiol Rev	10.1128/mr.43.2.260-296.1979	1979

References

#814	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 1537
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Methanococcus voltae (DSM 1537, ATCC 33273, OCM 70)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_024807035

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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