Methanobacterium alcaliphilum (DSM 3459, ATCC 43378, OCM 10)

Name: Methanobacterium alcaliphilum (DSM 3459, ATCC 43378, OCM 10)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 6902

Type strain

Strain Designation WeN3

Culture col. no. DSM 3459 ATCC 43378 OCM 10 WeN3

NCBI tax ID(s) 392018

Special Collections DSMZ

History <- D. R. Boone, University of California, School of Public Health, Los Angeles, USA; WeN3

Links

Methanobacterium alcaliphilum WeN3 is an anaerobe archaeon that was isolated from high-pH lake sediment.

anaerobe genome sequence Archaea

Name and taxonomic classification

SI-ID 41807

ATCC 43378,DSM 3459

@ref 20215

Last LPSN update 2026-02-09 11:19:20

Domain Archaea

Phylum Methanobacteriota

Class Methanobacteria

Order Methanobacteriales

Family Methanobacteriaceae

Genus Methanobacterium

Species Methanobacterium alcaliphilum

Full scientific name Methanobacterium alcaliphilum Worakit et al. 1986

BacDive ID	Other strains from **Methanobacterium alcaliphilum** (3)	Type strain
6903	M. alcaliphilum WeN4, DSM 3387, ATCC 43379, NBRC 105226, ... (type strain)
6900	M. alcaliphilum WeN1, DSM 3457, ATCC 43376, OCM 8
6901	M. alcaliphilum WeN2, DSM 3458, ATCC 43377, OCM 9

Morphology

Cell morphology

@ref	Motility	Confidence
125438		91

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
1317	METHANOBACTERIUM ALCALIPHILUM MEDIUM (DSMZ Medium 342)		Medium recipe at MediaDive	Name: METHANOBACTERIUM ALCALIPHILUM MEDIUM (DSMZ Medium 342) Composition: NaHCO3 9.88141 g/l Trypticase peptone 1.97628 g/l Yeast extract 1.97628 g/l NH4Cl 0.988145 g/l L-Cysteine HCl x H2O 0.494071 g/l Na2CO3 0.494071 g/l K2HPO4 0.395257 g/l MgCl2 x 6 H2O 0.0988145 g/l MgSO4 x 7 H2O 0.0296443 g/l Nitrilotriacetic acid 0.0148221 g/l NaCl 0.00988142 g/l MnSO4 x H2O 0.00494071 g/l ZnSO4 x 7 H2O 0.00177866 g/l CoSO4 x 7 H2O 0.00177866 g/l CaCl2 x 2 H2O 0.000988142 g/l FeSO4 x 7 H2O 0.000988142 g/l Sodium resazurin 0.000494071 g/l NiCl2 x 6 H2O 0.000296443 g/l AlK(SO4)2 x 12 H2O 0.000197628 g/l Na2MoO4 x 2 H2O 9.88142e-05 g/l H3BO3 9.88142e-05 g/l CuSO4 x 5 H2O 9.88142e-05 g/l Na2WO4 x 2 H2O 3.95257e-06 g/l Na2SeO3 x 5 H2O 2.96443e-06 g/l Tris-HCl buffer Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
1317	positive	growth	37

Physiology and metabolism

Oxygen tolerance

1317

Oxygen toleranceanaerobe

Spore formation

@ref	Spore formation	Confidence
125438		90.317

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Lake (large)
#Environmental	#Aquatic	#Sediment
#Condition	#Alkaline	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
1317	high-pH lake sediment	Wadi el Natrun	Egypt	EGY	Africa

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
1317	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM2322771v1 assembly for Methanobacterium alcaliphilum DSM 3459	contig	GCA_023227715	392018.4	8055140161	392018	62.28

Genome-based predictions

Predictions

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Methanobacterium alcaliphilum DSM 3459
NCBI: GCA_023227715.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	60.49	no
125438	anaerobic	anaerobicⓘ	yes	85.47	yes
125438	aerobic	aerobicⓘ	no	89.18	yes
125438	spore-forming	spore-formingⓘ	no	90.32	no
125438	thermophilic	thermophileⓘ	no	65.63	no
125438	flagellated	motile2+ⓘ	no	91.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Methanobacterium alkalithermotolerans sp. nov., a novel alkaliphilic and hydrogen-utilizing methanogen isolated from an alkaline geothermal spring (La Crouen, New Caledonia).	Mei N, Postec A, Bartoli M, Vandecasteele C, Wils L, Gil L, Monnin C, Pelletier B, Erauso G, Quemeneur M	Int J Syst Evol Microbiol	10.1099/ijsem.0.005554	2022

References

#1317	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 3459
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Methanobacterium alcaliphilum (DSM 3459, ATCC 43378, OCM 10)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_023227715

Genome-based predictions

Predictions

Literature

Literature

References

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