Methanobacterium alcaliphilum (DSM 3458, ATCC 43377, OCM 9)

Name: Methanobacterium alcaliphilum (DSM 3458, ATCC 43377, OCM 9)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 6901

Type strain

Strain Designation WeN2

Culture col. no. DSM 3458 ATCC 43377 OCM 9 WeN2

NCBI tax ID(s) 392018

Special Collections DSMZ

History <- D. R. Boone, University of California, School of Public Health, Los Angeles, USA; WeN2

Links

Methanobacterium alcaliphilum WeN2 is an anaerobe archaeon that was isolated from high-pH lake sediment.

anaerobe Archaea

Name and taxonomic classification

SI-ID 41806

ATCC 43377,DSM 3458

@ref 20215

Last LPSN update 2026-02-09 11:19:20

Domain Archaea

Phylum Methanobacteriota

Class Methanobacteria

Order Methanobacteriales

Family Methanobacteriaceae

Genus Methanobacterium

Species Methanobacterium alcaliphilum

Full scientific name Methanobacterium alcaliphilum Worakit et al. 1986

BacDive ID	Other strains from **Methanobacterium alcaliphilum** (3)	Type strain
6903	M. alcaliphilum WeN4, DSM 3387, ATCC 43379, NBRC 105226, ... (type strain)
6900	M. alcaliphilum WeN1, DSM 3457, ATCC 43376, OCM 8
6902	M. alcaliphilum WeN3, DSM 3459, ATCC 43378, OCM 10

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
1316	METHANOBACTERIUM ALCALIPHILUM MEDIUM (DSMZ Medium 342)		Medium recipe at MediaDive	Name: METHANOBACTERIUM ALCALIPHILUM MEDIUM (DSMZ Medium 342) Composition: NaHCO3 9.88141 g/l Trypticase peptone 1.97628 g/l Yeast extract 1.97628 g/l NH4Cl 0.988145 g/l L-Cysteine HCl x H2O 0.494071 g/l Na2CO3 0.494071 g/l K2HPO4 0.395257 g/l MgCl2 x 6 H2O 0.0988145 g/l MgSO4 x 7 H2O 0.0296443 g/l Nitrilotriacetic acid 0.0148221 g/l NaCl 0.00988142 g/l MnSO4 x H2O 0.00494071 g/l ZnSO4 x 7 H2O 0.00177866 g/l CoSO4 x 7 H2O 0.00177866 g/l CaCl2 x 2 H2O 0.000988142 g/l FeSO4 x 7 H2O 0.000988142 g/l Sodium resazurin 0.000494071 g/l NiCl2 x 6 H2O 0.000296443 g/l AlK(SO4)2 x 12 H2O 0.000197628 g/l Na2MoO4 x 2 H2O 9.88142e-05 g/l H3BO3 9.88142e-05 g/l CuSO4 x 5 H2O 9.88142e-05 g/l Na2WO4 x 2 H2O 3.95257e-06 g/l Na2SeO3 x 5 H2O 2.96443e-06 g/l Tris-HCl buffer Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
1316	positive	growth	37

Physiology and metabolism

Oxygen tolerance

1316

Oxygen toleranceanaerobe

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Lake (large)
#Environmental	#Aquatic	#Sediment
#Condition	#Alkaline	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
1316	high-pH lake sediment	Wadi el Natrun	Egypt	EGY	Africa

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
1316	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome-based predictions

Literature

References

#1316	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 3458
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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