Name: Leuconostoc citreum B2399
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 6828

Type strain:

Species: Leuconostoc citreum

Strain Designation: B2399

Culture col. no.: DSM 5577, ATCC 49370, NCDO 1837, CCUG 30060, JCM 9698, CCM 4544, CECT 4025, CGMCC 1.2461, CIP 103315, KCTC 3526, LMG 9849, NCFB 1837, NCIMB 13121, NRIC 1776, VTT E-93504

Strain history: CIP <- 1989, NCFB <- 1966, A.C. Hayward, Kew, UK: strain B2399

NCBI tax ID(s): 33964 (species)

SI-ID 3489

NCFB 1837, LMG 9849, ATCC 49370, CCUG 30060, NCDO 1837, CIP 103315, DSM 5577, JCM 9698, CECT 4025, NCIMB 13121, CCM4544, VTT E-93504, KCTC 3526, CGMCC 1.2461

Special Collections

Other strains from Leuconostoc citreum

Section

Leuconostoc citreum B2399 is a facultative anaerobe, mesophilic, Gram-positive bacterium that was isolated from honey dew of rye ear.

Gram-positive
ovoid-shaped
facultative anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Lactobacillaceae*
	Genus	*Leuconostoc*
	Species	**Leuconostoc citreum**
	Full Scientific Name (LPSN)	Leuconostoc citreum Farrow et al. 1989

Synonym

Leuconostoc amelibiosum

Information on morphological and physiological properties Morphology

[Ref.: #118298]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %94.621

[Ref.: #118298]	Cell shape	ovoid-shaped

[Ref.: #118298]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %91

Information on culture and growth conditions Culture and growth conditions

[Ref.: #2229]

Culture medium

MRS MEDIUM (DSMZ Medium 11)

[Ref.: #2229]

Culture medium growth

[Ref.: #2229]

Culture medium link

Medium recipe at MediaDive

[Ref.: #2229]

Culture medium composition

expand / minimize

[Ref.: #34244]

Culture medium

MEDIUM 40- for Lactobacillus and Leuconostoc

[Ref.: #34244]

Culture medium growth

[Ref.: #34244]

Culture medium composition

expand / minimize

[Ref.: #118298]

Culture medium

CIP Medium 40

[Ref.: #118298]

Culture medium growth

[Ref.: #118298]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #2229]		growth	28 °C
[Ref.: #34244]		growth	30 °C
[Ref.: #49880]		growth	30 °C
[Ref.: #67770]		growth	30 °C
[Ref.: #118298]		growth	10-37 °C
[Ref.: #118298]	no	growth	45 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #118298]

Oxygen tolerance

facultative anaerobe

[Ref.: #69481]

Ability of spore formation

no Confidence in %100

	Salt	Tested relation		Salt conc.
[Ref.: #118298]	NaCl		growth	0-4	%
[Ref.: #118298]	NaCl	no	growth	6	%
[Ref.: #118298]	NaCl	no	growth	8	%
[Ref.: #118298]	NaCl	no	growth	10	%

[Ref.: #2229]	Murein short key	A11.05
[Ref.: #2229]	Murein types	A3alpha L-Lys-L-Ala₂

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	arbutin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #118298]	citrate ChEBI	-	carbon source
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	esculin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	maltose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #118298]	nitrate ChEBI	-	reduction
[Ref.: #118298]	nitrate ChEBI	+	respiration
[Ref.: #118298]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #118298]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	no	yes

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #118298]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #118298]	caseinase	-	3.4.21.50
[Ref.: #118298]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #118298]	gelatinase	-
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #118298]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #118298]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #118298]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #49880]

Fatty acid	Percentage	ECL
C16:0	38.6	16
C19:0 CYCLO ω8c	27.9	18.9
C18:2 ω6,9c/C18:0 ANTE	12.5	17.724
C16:1 ω7c	8.5	15.819
C18:1 ω7c /12t/9t	7.5	17.824
C17:0 CYCLO	2.7	16.888
C14:0	2.3	14

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #2229]	15032	-	-	-	-	+	-	-	-	-	-	-	+	+	+	-	-	-	-	-	-	-	+	+	+	+	+	+	+	+	-	-	+	+	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-
[Ref.: #118298]	9246	not determinedn.d.	-	-	-	+	-	-	-	-	-	-	+	+	+	-	-	-	-	-	-	-	+	+	-	+	+	+	-	+	-	-	+	+	-	-	-	-	-	-	+/-	+	-	-	-	-	-	-	+	+	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #118298]	7494	-	-	+	-	-	-	-	-	-	-	+	+	-	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #2229]	Sample type/isolated from	honey dew of rye ear

[Ref.: #49880]	Sample type/isolated from	Honey-dew of rye ear

[Ref.: #67770]	Sample type/isolated from	Honeydew of rye ear

[Ref.: #118298]	Sample type/isolated from	Plant, Honey-dew of rye ear
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Fruit (Seed)
#Host Body Product	#Plant	#Plant exudate (Resin)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LC096222 (>99% sequence identity) for Leuconostoc from Microbeatlas

Aquatic Samples	3075
Soil Samples	1568
Animal Samples	19457
Plant Samples	1324
Total Samples	25424

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #2229]	Pathogenicity (human)	yes, in single cases
[Ref.: #2229]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #118298]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Leuconostoc citreum gene for 16S ribosomal RNA, partial sequence, strain: JCM 9698	LC096222	1491	GenBank ENA	33964 tax ID
[Ref.: #20218]	Leuconostoc citreum 16S ribosomal RNA gene, partial sequence	AF111948	1505	GenBank ENA	33964 tax ID	*
[Ref.: #20218]	Leuconostoc citreum gene for 16S rRNA, strain:NRIC 1776	AB022923	1448	GenBank ENA	33964 tax ID	*
[Ref.: #20218]	Leuconostoc citreum DNA, 16S-23S rRNA intergenic spacer region, strain: NRIC 1776	AB290437	377	GenBank ENA	33964 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Leuconostoc citreum ATCC 49370	GCA_004354555	contig	GenBank ENA	33964 tax ID
[Ref.: #66792]	Leuconostoc citreum NRIC 1776	GCA_008326545	scaffold	GenBank ENA	33964 tax ID	*
[Ref.: #66792]	Leuconostoc citreum strain ATCC 49370	33964.176	wgs	PATRIC	33964 tax ID	*
[Ref.: #66792]	Leuconostoc citreum strain NRIC 1776	33964.186	wgs	PATRIC	33964 tax ID	*

[Ref.: #2229]	GC-content	39.8 mol%
[Ref.: #67770]	GC-content	39.8 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Leuconostoc citreum NRIC 1776 NCBI: GCA_008326545.1
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Leuconostoc citreum NRIC 1776 NCBI: GCA_008326545.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	94.621	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	86.784	yes
[Ref.: #69480]	aerobic	aerobicⓘ	no	90.412	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	89.26	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	97.956	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	91	no

Availability in culture collections External links

[Ref.: #2229]

Culture collection no.

DSM 5577, ATCC 49370, NCDO 1837, CCUG 30060, JCM 9698, CCM 4544, CECT 4025, CGMCC 1.2461, CIP 103315, KCTC 3526, LMG 9849, NCFB 1837, NCIMB 13121, NRIC 1776, VTT E-93504

[Ref.: #76293]

SI-ID 3489

Literature:

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Mannitol production by lactic acid bacteria grown in supplemented carob syrup.	Carvalheiro F, Moniz P, Duarte LC, Esteves MP, Girio FM	J Ind Microbiol Biotechnol	10.1007/s10295-010-0823-5	2010	*
Phylogeny	Leuconostoc palmae sp. nov., a novel lactic acid bacterium isolated from palm wine.	Ehrmann MA, Freiding S, Vogel RF	Int J Syst Evol Microbiol	10.1099/ijs.0.005983-0	2009	*
Enzymology	Expression of alpha-galactosidase gene from Leuconostoc mesenteroides SY1 in Leuconostoc citreum.	Park JY, Jeong SJ, Lee AR, Park JY, Jeong WJ, Kim JH	J Microbiol Biotechnol	7090	2007	*
Phylogeny	Leuconostoc kimchii sp. nov., a new species from kimchi.	Kim J, Chun J, Han HU	Int J Syst Evol Microbiol	10.1099/00207713-50-5-1915	2000	*

References

#2229

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 5577

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#34244 ; Curators of the CIP;
#49880 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 30060

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#76293

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID3489.1 )

#118298 Collection of Institut Pasteur ; Curators of the CIP; CIP 103315
* These data were automatically processed and therefore are not curated

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Search for species Leuconostoc citreum in external resources:
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Leuconostoc citreum NRIC 1776

Leuconostoc citreum NRIC 1776

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Change proposal