Name: Leuconostoc citreum B-742
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 6829

Type strain:

Species: Leuconostoc citreum

Strain Designation: B-742

Culture col. no.: DSM 20188, ATCC 13146, NRRL B-742, CCUG 30058, JCM 9699, CECT 4018, CIP 103405, KCTC 3524, LMG 9824, NCIMB 13227, NRIC 1772

Strain history: CIP <- 1990, DSM, Leuconostoc sp. <- W. Holzapfel, Leuconostoc mesenteroides subsp. amelibiosum <- ATCC <- NRRL: strain B-742

NCBI tax ID(s): 33964 (species)

SI-ID 125690

LMG 9824, ATCC 13146, CCUG 30058, DSM 20188, LMG 18974, NCFB 2787, NCIMB 13227, NRRL B-742, CIP 103405, JCM 9699, CECT 4018, KCTC 3524, VTT E-093125

Other strains from Leuconostoc citreum

L. citreum B2399, DSM 5577, ATCC 49370, NCDO ... (type strain)
L. citreum GL 87, DSM 15948
L. citreum CIP 102218
L. citreum 5, CIP 103326, NCIB 3351, NRRL ...
L. citreum CIP 106143, ATCC 10882
L. citreum CIP 106146, ATCC 11449, NRRL B-1299
L. citreum
L. citreum CCUG 30586
L. citreum CCUG 34887
L. citreum CCUG 39492
L. citreum CCUG 39979
L. citreum CCUG 51260
L. citreum JCM 12651
L. citreum JCM 20062, ATU L-24, IAM 1122
L. citreum JCM 21183, IAM 14400, NBRC 102476, ...

Section

Leuconostoc citreum B-742 is an anaerobe, mesophilic, Gram-positive human pathogen of the family Lactobacillaceae.

Gram-positive
oval-shaped
anaerobe
human pathogen
mesophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Lactobacillaceae*
	Genus	*Leuconostoc*
	Species	**Leuconostoc citreum**
	Full Scientific Name (LPSN)	Leuconostoc citreum Farrow et al. 1989

Synonym

Leuconostoc amelibiosum

Information on morphological and physiological properties Morphology

[Ref.: #118299]	Gram stain	positive

[Ref.: #118299]	Cell shape	oval-shaped

[Ref.: #118299]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #8463]

Culture medium

MRS MEDIUM (DSMZ Medium 11)

[Ref.: #8463]

Culture medium growth

[Ref.: #8463]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8463]

Culture medium composition

expand / minimize

[Ref.: #41342]

Culture medium

MEDIUM 40- for Lactobacillus and Leuconostoc

[Ref.: #41342]

Culture medium growth

[Ref.: #41342]

Culture medium composition

expand / minimize

[Ref.: #118299]

Culture medium

CIP Medium 41

[Ref.: #118299]

Culture medium growth

[Ref.: #118299]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #118299]

Culture medium

CIP Medium 40

[Ref.: #118299]

Culture medium growth

[Ref.: #118299]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #8463]	growth	30 °C
[Ref.: #41342]	growth	37 °C
[Ref.: #67770]	growth	30 °C

[Ref.: #8463]	Temperature range	mesophilic
[Ref.: #41342]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #49878]	Oxygen tolerance	anaerobe
[Ref.: #49878]	Oxygen tolerance	microaerophile

[Ref.: #8463]	Murein short key	A11.05
[Ref.: #8463]	Murein types	A3alpha L-Lys-L-Ala₂

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	cellobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	esculin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	maltose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #118299]	nitrate ChEBI	-	reduction
[Ref.: #118299]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #118299]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #118299]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #118299]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #118299]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #49878]

Fatty acid	Percentage	ECL
C16:0	38.1	16
C19:0 CYCLO ω8c	30.3	18.9
C19:0 CYCLO ω9c	10.3	18.87
C18:0	6.3	18
C18:2 ω6,9c/C18:0 ANTE	5.4	17.724
C17:0 CYCLO	4	16.888
C16:1 ω7c	3.1	15.819
C14:0	1.4	14
C18:1 ω9c	1.2	17.769

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #8463]	14549	-	-	-	-	+	-	-	-	-	-	-	+	+	+	-	-	-	-	+/-	-	-	-	+	-	-	+	-	-	+	-	-	+	+	-	-	-	-	-	-	-	+	-	-	-	-	-	-	+	-	-
[Ref.: #118299]	9262	not determinedn.d.	-	-	-	+/-	-	-	-	-	-	-	+	+	+/-	-	-	-	-	-	-	-	-	+	-	-	+	-	-	+	-	-	+	+	-	-	-	-	-	-	-	+	-	-	-	-	-	-	+	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #118299]	7521	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #69479]

Global distribution of 16S sequence LC260024 (>99% sequence identity) for Leuconostoc from Microbeatlas

Aquatic Samples	3075
Soil Samples	1568
Animal Samples	19457
Plant Samples	1324
Total Samples	25424

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #8463]	Pathogenicity (human)	yes, in single cases
[Ref.: #8463]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #118299]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Leuconostoc citreum gene for 16S ribosomal RNA, partial sequence, strain: JCM 9699	LC260024	1488	ENA	33964 tax ID

[Ref.: #8463]

GC-content

41.6 mol%

Availability in culture collections External links

[Ref.: #8463]

Culture collection no.

DSM 20188, ATCC 13146, NRRL B-742, CCUG 30058, JCM 9699, CECT 4018, CIP 103405, KCTC 3524, LMG 9824, NCIMB 13227, NRIC 1772

[Ref.: #76294]

SI-ID 125690

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Complete Genome Sequence of Leuconostoc citreum Strain NRRL B-742.	Passerini D, Vuillemin M, Laguerre S, Amari M, Loux V, Gabriel V, Robert H, Morel S, Monsan P, Gabriel B, Fontagne-Faucher C, Remaud-Simeon M, Moulis C	Genome Announc	10.1128/genomeA.01179-14	2014	*
Enzymology	Stability Study of Crude Dextransucrase from Leuconostoc citreum NRRL B-742.	Rabelo MC, Fontes CM, Rodrigues S	Indian J Microbiol	10.1007/s12088-011-0114-5	2011	*
Metabolism	Functional differences in Leuconostoc sensitive and resistant strains to mesenterocin 52A, a class IIa bacteriocin.	Jasniewski J, Cailliez-Grimal C, Younsi M, Milliere JB, Revol-Junelles AM	FEMS Microbiol Lett	10.1111/j.1574-6968.2008.01381.x	2008	*

References

#8463

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20188

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#41342 ; Curators of the CIP;
#49878 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 30058
#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#76294

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID125690.1 )

#118299 Collection of Institut Pasteur ; Curators of the CIP; CIP 103405
* These data were automatically processed and therefore are not curated

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