Legionella maceachernii (DSM 16642, ATCC 35300, NCTC 11982)

Name: Legionella maceachernii (DSM 16642, ATCC 35300, NCTC 11982)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 6793

Type strain

Strain Designation PX-1-G2-E2

Culture col. no. DSM 16642 ATCC 35300 NCTC 11982 CCUG 31116 CIP 103846 2 more

NCBI tax ID(s) 466

Special Collections DSMZ CCUG JCM CIP

History <- E. Müller <- ATCC <- G. W. Gorman GIFU 10745 <-- CDC PX-1-G2-E2. CIP <- 1993, R.F. Benson, CDC: strain PX-1-G2-E2 <- G.W. Gorman, Legionella maceachernii

Links

Legionella maceachernii PX-1-G2-E2 is a microaerophile, Gram-negative, rod-shaped bacterium that was isolated from water in home evaporator-cooler.

Gram-negative rod-shaped microaerophile genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 40981

ATCC 35300,NCTC 11982,CCUG 31116,CIP 103846,JCM 7566,DSM 16642

@ref 20215

Last LPSN update 2026-02-09 11:19:19

Domain Bacteria

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Legionellales

Family Legionellaceae

Genus Legionella

Species Legionella maceachernii

Full scientific name Legionella maceachernii Brenner et al. 1985

Synonyms (1)

Tatlockia maceachernii

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
120732	negative	rod-shaped
125438	negative		96.994
125439	negative		95.9

Colony morphology

6545

Incubation period2-3 days

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
6545	BCYE-AGAR (DSMZ Medium 585)	Medium recipe at MediaDive	Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base
34035	MEDIUM 23 - for Afipia and Legionella		Distilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml)
120732	CIP Medium 23	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
6545	positive	growth	37
34035	positive	growth	37
50432	positive	growth	37
67770	positive	growth	37

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
6545	microaerophile
125439	obligate aerobe	98.6

Spore formation

@ref	Spore formation	Confidence
125438		90.796
125439		97.4

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
120732	17632 ChEBI	nitrate	-	reduction
120732	16301 ChEBI	nitrite	-	reduction

Metabolite production

@ref	Chebi-ID	Metabolite	Production
120732	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
120732	catalase	-	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase lipase (C 8)	-		from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
120732	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
120732	urease	-	3.5.1.5

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
120732	-	+	-	-	-	+	+	-	-	-	+	+	-	-	-	-	-	-	-	-
6545	-	+/-	+/-	-	-	-	+/-	-	-	-	+/-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Industrial	#Engineered product
#Environmental	#Aquatic	-

Isolation

@ref	Sample type	Sampling date	Geographic location	Country	Country ISO 3 Code	Continent
6545	water in home evaporator-cooler		Arizona	USA	USA	North America
50432	Water in home evaporator cooler	1979	Phoenix	USA	USA	North America
67770	Water, home evaporator-cooler
120732	Environment, Water in home evaporator cooler		Phoenix, Arizona	United States of America	USA	North America

Taxonmaps

69479

Global distribution of 16S sequence NR_041790 (>99% sequence identity) for Legionella maceachernii from Microbeatlas

Aquatic Samples	576
Soil Samples	461
Animal Samples	120
Plant Samples	142
Total Samples	1299

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
6545	2	Risk group (German classification) According to TRBA 466
120732	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	50592_A01 assembly for Legionella maceachernii NCTC11982	contig	GCA_900460175	466.12	2958701271	466	76.88
67770	IMG-taxon 2585428143 annotated assembly for Legionella maceachernii ATCC 35300	scaffold	GCA_900167045	466.9	2585428143	466	69.04
66792	ASM146784v1 assembly for Legionella maceachernii PX-1-G2-E2	scaffold	GCA_001467845	466.6	2660238140	466	57.7

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Legionella maceachernii 16S ribosomal RNA gene, partial sequence	AF227161	1474	466
20218	Legionella maceachernii strain MDC1610 16S ribosomal RNA gene, partial sequence	JF720368	526	466
20218	L.maceachernii gene for 16S ribosomal RNA	X60081	1453	466
6545	Legionella maceachernii strain ATCC 35300 16S ribosomal RNA, partial sequence	NR_041790	1474	466
124043	Legionella maceachernii 16S-23S ribosomal RNA intergenic spacer, partial sequence and tRNA-Ile gene, complete sequence.	KM610004	544	466
124043	Legionella maceachernii 16S-23S ribosomal RNA intergenic spacer, partial sequence and tRNA-Ala gene, complete sequence.	KM610003	750	466

GC content

@ref	GC-content (mol%)	Method
67770	43	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Legionella maceachernii PX-1-G2-E2
NCBI: GCA_001467845

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	97.40	no
125439	motility	BacteriaNetⓘ	no	55.50	no
125439	gram_stain	BacteriaNetⓘ	negative	95.90	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	98.60	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Legionella maceachernii PX-1-G2-E2
NCBI: GCA_001467845.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	96.99	no
125438	anaerobic	anaerobicⓘ	no	95.70	yes
125438	aerobic	aerobicⓘ	no	69.25	yes
125438	spore-forming	spore-formingⓘ	no	90.80	no
125438	thermophilic	thermophileⓘ	no	91.17	no
125438	flagellated	motile2+ⓘ	no	58.06	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Legionella confirmation using real-time PCR and SYTO9 is an alternative to current methodology.	Giglio S, Monis PT, Saint CP.	Appl Environ Microbiol	10.1128/aem.71.12.8944-8948.2005	2005
Enzymology	Fatal Legionella maceachernii pneumonia.	Wilkinson HW, Thacker WL, Brenner DJ, Ryan KJ.	J Clin Microbiol	10.1128/jcm.22.6.1055-.1985	1985
Enzymology	Development and evaluation of Chlamylege, a new commercial test allowing simultaneous detection and identification of Legionella, Chlamydophila pneumoniae, and Mycoplasma pneumoniae in clinical respiratory specimens by multiplex PCR.	Ginevra C, Barranger C, Ros A, Mory O, Stephan JL, Freymuth F, Joannes M, Pozzetto B, Grattard F.	J Clin Microbiol	10.1128/jcm.43.7.3247-3254.2005	2005
Pathogenicity	Susceptibility of Legionella strains to the chlorinated biocide, monochloramine.	Jakubek D, Guillaume C, Binet M, Leblon G, DuBow M, Le Brun M.	Microbes Environ	10.1264/jsme2.me12205	2013
Metabolism	Targeting species-specific low-affinity 16S rRNA binding sites by using peptide nucleic acids for detection of Legionellae in biofilms.	Wilks SA, Keevil CW.	Appl Environ Microbiol	10.1128/aem.02918-05	2006
Biotechnology	Detection of Salmonella spp. in oysters by PCR.	Bej AK, Mahbubani MH, Boyce MJ, Atlas RM.	Appl Environ Microbiol	10.1128/aem.60.1.368-373.1994	1994
	Genus-specific epitope on the 60-kilodalton Legionella heat shock protein recognized by a monoclonal antibody.	Steinmetz I, Rheinheimer C, Hubner I, Bitter-Suermann D.	J Clin Microbiol	10.1128/jcm.29.2.346-354.1991	1991
	Compilation of small ribosomal subunit RNA structures.	Neefs JM, Van de Peer Y, De Rijk P, Chapelle S, De Wachter R.	Nucleic Acids Res	10.1093/nar/21.13.3025	1993

References

#6545	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 16642
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#34035	; Curators of the CIP;
#50432	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 31116
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#120732	Collection of Institut Pasteur ; Curators of the CIP; CIP 103846
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Rate our new design

Content

About us

Legionella maceachernii (DSM 16642, ATCC 35300, NCTC 11982)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Metabolite utilization

Metabolite production

Enzymes

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_900460175

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

Help

Social Media