Strain identifier

BacDive ID: 6793

Type strain: Yes

Species: Legionella maceachernii

Strain Designation: PX-1-G2-E2

Strain history: CIP <- 1993, R.F. Benson, CDC: strain PX-1-G2-E2 <- G.W. Gorman, Legionella maceachernii

NCBI tax ID(s): 466 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 6545

BacDive-ID: 6793

DSM-Number: 16642

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-negative

description: Legionella maceachernii PX-1-G2-E2 is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from water in home evaporator-cooler.

NCBI tax id

  • NCBI tax id: 466
  • Matching level: species

strain history

@refhistory
6545<- E. Müller <- ATCC <- G. W. Gorman
67770GIFU 10745 <-- CDC PX-1-G2-E2.
120732CIP <- 1993, R.F. Benson, CDC: strain PX-1-G2-E2 <- G.W. Gorman, Legionella maceachernii

doi: 10.13145/bacdive6793.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Legionellales
  • family: Legionellaceae
  • genus: Legionella
  • species: Legionella maceachernii
  • full scientific name: Legionella maceachernii Brenner et al. 1985
  • synonyms

    • @ref: 20215
    • synonym: Tatlockia maceachernii

@ref: 6545

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Legionellales

family: Legionellaceae

genus: Legionella

species: Legionella maceachernii

full scientific name: Legionella maceachernii Brenner et al. 1985

strain designation: PX-1-G2-E2

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480negative99.999
120732negativerod-shapedno

colony morphology

  • @ref: 6545
  • incubation period: 2-3 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6545BCYE-AGAR (DSMZ Medium 585)yeshttps://mediadive.dsmz.de/medium/585Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base
34035MEDIUM 23 - for Afipia and LegionellayesDistilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml)
120732CIP Medium 23yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=23

culture temp

@refgrowthtypetemperaturerange
6545positivegrowth37mesophilic
34035positivegrowth37mesophilic
50432positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 6545
  • oxygen tolerance: microaerophile

spore formation

@refspore formationconfidence
69481no99
69480no99.83

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12073217632nitrate-reduction
12073216301nitrite-reduction

metabolite production

  • @ref: 120732
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382lipase (C 14)-
68382esterase lipase (C 8)-
120732oxidase-
120732catalase-1.11.1.6
120732urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
6545-+/-+/----+/----+/----------
120732-+---++---++--------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling date
6545water in home evaporator-coolerArizonaUSAUSANorth America
50432Water in home evaporator coolerPhoenixUSAUSANorth America1979
67770Water, home evaporator-cooler
120732Environment, Water in home evaporator coolerPhoenix, ArizonaUnited States of AmericaUSANorth America

isolation source categories

Cat1Cat2Cat3
#Engineered#Industrial#Engineered product
#Environmental#Aquatic

taxonmaps

  • @ref: 69479
  • File name: preview.99_1192.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_35;96_306;97_340;98_383;99_1192&stattab=map
  • Last taxonomy: Legionella maceachernii
  • 16S sequence: NR_041790
  • Sequence Identity:
  • Total samples: 1299
  • soil counts: 461
  • aquatic counts: 576
  • animal counts: 120
  • plant counts: 142

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
65452Risk group (German classification)
1207321Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Legionella maceachernii 16S ribosomal RNA gene, partial sequenceAF2271611474ena466
20218Legionella maceachernii strain MDC1610 16S ribosomal RNA gene, partial sequenceJF720368526ena466
20218L.maceachernii gene for 16S ribosomal RNAX600811453ena466
6545Legionella maceachernii strain ATCC 35300 16S ribosomal RNA, partial sequenceNR_0417901474nuccore466

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Legionella maceachernii PX-1-G2-E2GCA_001467845scaffoldncbi466
66792Legionella maceachernii strain ATCC 35300466.9wgspatric466
66792Legionella maceachernii strain NCTC11982466.12wgspatric466
66792Legionella maceachernii strain PX-1-G2-E2466.6wgspatric466
66792Legionella maceachernii ATCC 353002585428143draftimg466
67770Legionella maceachernii ATCC 35300GCA_900167045scaffoldncbi466
67770Legionella maceachernii NCTC11982GCA_900460175contigncbi466

GC content

  • @ref: 67770
  • GC-content: 43
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno99no
flagellatedno87.846no
gram-positiveno98.61no
anaerobicno95.838yes
aerobicno90.406yes
halophileno86.627no
spore-formingno93.47no
glucose-utilyes60.942no
motileyes57.685no
thermophileno98.12yes
glucose-fermentno86.644no

External links

@ref: 6545

culture collection no.: DSM 16642, ATCC 35300, NCTC 11982, CCUG 31116, CIP 103846, JCM 7566

straininfo link

  • @ref: 76263
  • straininfo: 40981

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
6545Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16642)https://www.dsmz.de/collection/catalogue/details/culture/DSM-16642
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
34035Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15688
50432Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 31116)https://www.ccug.se/strain?id=31116
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
76263Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID40981.1StrainInfo: A central database for resolving microbial strain identifiers
120732Curators of the CIPCollection of Institut Pasteur (CIP 103846)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103846