Strain identifier
BacDive ID: 6793
Type strain:
Species: Legionella maceachernii
Strain Designation: PX-1-G2-E2
Strain history: CIP <- 1993, R.F. Benson, CDC: strain PX-1-G2-E2 <- G.W. Gorman, Legionella maceachernii
NCBI tax ID(s): 466 (species)
General
@ref: 6545
BacDive-ID: 6793
DSM-Number: 16642
keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-negative
description: Legionella maceachernii PX-1-G2-E2 is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from water in home evaporator-cooler.
NCBI tax id
- NCBI tax id: 466
- Matching level: species
strain history
@ref | history |
---|---|
6545 | <- E. Müller <- ATCC <- G. W. Gorman |
67770 | GIFU 10745 <-- CDC PX-1-G2-E2. |
120732 | CIP <- 1993, R.F. Benson, CDC: strain PX-1-G2-E2 <- G.W. Gorman, Legionella maceachernii |
doi: 10.13145/bacdive6793.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Legionellales
- family: Legionellaceae
- genus: Legionella
- species: Legionella maceachernii
- full scientific name: Legionella maceachernii Brenner et al. 1985
synonyms
- @ref: 20215
- synonym: Tatlockia maceachernii
@ref: 6545
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Legionellales
family: Legionellaceae
genus: Legionella
species: Legionella maceachernii
full scientific name: Legionella maceachernii Brenner et al. 1985
strain designation: PX-1-G2-E2
type strain: yes
Morphology
cell morphology
@ref | gram stain | confidence | cell shape | motility |
---|---|---|---|---|
69480 | negative | 99.999 | ||
120732 | negative | rod-shaped | no |
colony morphology
- @ref: 6545
- incubation period: 2-3 days
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
6545 | BCYE-AGAR (DSMZ Medium 585) | yes | https://mediadive.dsmz.de/medium/585 | Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base |
34035 | MEDIUM 23 - for Afipia and Legionella | yes | Distilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml) | |
120732 | CIP Medium 23 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=23 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
6545 | positive | growth | 37 | mesophilic |
34035 | positive | growth | 37 | mesophilic |
50432 | positive | growth | 37 | mesophilic |
67770 | positive | growth | 37 | mesophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 6545
- oxygen tolerance: microaerophile
spore formation
@ref | spore formation | confidence |
---|---|---|
69481 | no | 99 |
69480 | no | 99.83 |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
120732 | 17632 | nitrate | - | reduction |
120732 | 16301 | nitrite | - | reduction |
metabolite production
- @ref: 120732
- Chebi-ID: 35581
- metabolite: indole
- production: no
enzymes
@ref | value | activity | ec |
---|---|---|---|
68382 | alpha-fucosidase | - | 3.2.1.51 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | trypsin | - | 3.4.21.4 |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | lipase (C 14) | - | |
68382 | esterase lipase (C 8) | - | |
120732 | oxidase | - | |
120732 | catalase | - | 1.11.1.6 |
120732 | urease | - | 3.5.1.5 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
6545 | - | +/- | +/- | - | - | - | +/- | - | - | - | +/- | - | - | - | - | - | - | - | - | - |
120732 | - | + | - | - | - | + | + | - | - | - | + | + | - | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | sampling date |
---|---|---|---|---|---|---|
6545 | water in home evaporator-cooler | Arizona | USA | USA | North America | |
50432 | Water in home evaporator cooler | Phoenix | USA | USA | North America | 1979 |
67770 | Water, home evaporator-cooler | |||||
120732 | Environment, Water in home evaporator cooler | Phoenix, Arizona | United States of America | USA | North America |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Engineered | #Industrial | #Engineered product |
#Environmental | #Aquatic |
taxonmaps
- @ref: 69479
- File name: preview.99_1192.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_35;96_306;97_340;98_383;99_1192&stattab=map
- Last taxonomy: Legionella maceachernii
- 16S sequence: NR_041790
- Sequence Identity:
- Total samples: 1299
- soil counts: 461
- aquatic counts: 576
- animal counts: 120
- plant counts: 142
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
6545 | 2 | Risk group (German classification) |
120732 | 1 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Legionella maceachernii 16S ribosomal RNA gene, partial sequence | AF227161 | 1474 | ena | 466 |
20218 | Legionella maceachernii strain MDC1610 16S ribosomal RNA gene, partial sequence | JF720368 | 526 | ena | 466 |
20218 | L.maceachernii gene for 16S ribosomal RNA | X60081 | 1453 | ena | 466 |
6545 | Legionella maceachernii strain ATCC 35300 16S ribosomal RNA, partial sequence | NR_041790 | 1474 | nuccore | 466 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Legionella maceachernii PX-1-G2-E2 | GCA_001467845 | scaffold | ncbi | 466 |
66792 | Legionella maceachernii strain ATCC 35300 | 466.9 | wgs | patric | 466 |
66792 | Legionella maceachernii strain NCTC11982 | 466.12 | wgs | patric | 466 |
66792 | Legionella maceachernii strain PX-1-G2-E2 | 466.6 | wgs | patric | 466 |
66792 | Legionella maceachernii ATCC 35300 | 2585428143 | draft | img | 466 |
67770 | Legionella maceachernii ATCC 35300 | GCA_900167045 | scaffold | ncbi | 466 |
67770 | Legionella maceachernii NCTC11982 | GCA_900460175 | contig | ncbi | 466 |
GC content
- @ref: 67770
- GC-content: 43
- method: thermal denaturation, midpoint method (Tm)
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 99 | no |
flagellated | no | 87.846 | no |
gram-positive | no | 98.61 | no |
anaerobic | no | 95.838 | yes |
aerobic | no | 90.406 | yes |
halophile | no | 86.627 | no |
spore-forming | no | 93.47 | no |
glucose-util | yes | 60.942 | no |
motile | yes | 57.685 | no |
thermophile | no | 98.12 | yes |
glucose-ferment | no | 86.644 | no |
External links
@ref: 6545
culture collection no.: DSM 16642, ATCC 35300, NCTC 11982, CCUG 31116, CIP 103846, JCM 7566
straininfo link
- @ref: 76263
- straininfo: 40981
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
6545 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16642) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-16642 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
34035 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/15688 | ||||
50432 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 31116) | https://www.ccug.se/strain?id=31116 | |||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68382 | Automatically annotated from API zym | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
76263 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID40981.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
120732 | Curators of the CIP | Collection of Institut Pasteur (CIP 103846) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103846 |