Legionella shakespearei (DSM 23087, ATCC 49655, CCUG 31228 A)

Name: Legionella shakespearei (DSM 23087, ATCC 49655, CCUG 31228 A)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 6760

Type strain

Strain Designation 214

Culture col. no. DSM 23087 ATCC 49655 CCUG 31228 A CIP 103541 NCTC 12829 2 more

NCBI tax ID(s) 1122169 45075

Special Collections DSMZ CCUG CIP

History DSMZ <- CIP <- W. L. Thacker, CDC <- U. K. Verma, Houseman Ltd., Slough, U.K.; 214 CIP <- 2011, CCUG <- 1993, R.F. Benson, CDC, Atlanta, USA: strain CDC 214

Links

Legionella shakespearei 214 is a microaerophile bacterium that was isolated from water .

microaerophile genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 42605

ATCC 49655,CCUG 31228 A,CIP 103541,NCTC 12829,DSM 23087

@ref 20215

Last LPSN update 2026-05-29 08:26:29

Domain Bacteria

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Legionellales

Family Legionellaceae

Genus Legionella

Species Legionella shakespearei

Full scientific name Legionella shakespearei Verma et al. 1992

Morphology

Cell morphology

@ref	Gram stain	Confidence
125438	negative	99.411
125439	negative	99.561

Colony morphology

@ref	Incubation period
16572	2-3 days
50484	3 days

Multimedia

@ref:	66793
multimedia content:	EM_DSM_23087_1.jpg
multimedia.multimedia content:	EM_DSM_23087_1.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

@ref:	66793
multimedia content:	EM_DSM_23087_2.jpg
multimedia.multimedia content:	EM_DSM_23087_2.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

@ref:	66793
multimedia content:	EM_DSM_23087_3.jpg
multimedia.multimedia content:	EM_DSM_23087_3.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

@ref:	66793
multimedia content:	EM_DSM_23087_4.jpg
multimedia.multimedia content:	EM_DSM_23087_4.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

@ref:	66793
multimedia content:	EM_DSM_23087_5.jpg
multimedia.multimedia content:	EM_DSM_23087_5.jpg
caption:	electron microscopic image
intellectual property rights:	© HZI/Manfred Rohde
manual_annotation:	1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
16572	BCYE-AGAR (DSMZ Medium 585)	Medium recipe at MediaDive	Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base
34345	MEDIUM 23 - for Afipia and Legionella		Distilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml)
122579	CIP Medium 23	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
16572	positive	growth	30
34345	positive	growth	37
50484	positive	growth	30

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
16572	microaerophile
50484	aerobe
125439	obligate aerobe	97.749

Spore formation

@ref	Spore formation	Confidence
125438		90.541
125439		99.951

Enzymes

@ref	Value	Activity	Ec
16572	catalase	+	1.11.1.6
16572	cytochrome-c oxidase	-	1.9.3.1

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	formaldehyde oxidation	100	3 of 3
66794	coenzyme A metabolism	100	4 of 4
66794	octane oxidation	100	3 of 3
66794	cardiolipin biosynthesis	100	7 of 7
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	ubiquinone biosynthesis	100	7 of 7
66794	acetate fermentation	100	4 of 4
66794	adipate degradation	100	2 of 2
66794	palmitate biosynthesis	100	22 of 22
66794	suberin monomers biosynthesis	100	2 of 2
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	folate polyglutamylation	100	1 of 1
66794	phenylalanine metabolism	92.31	12 of 13
66794	threonine metabolism	90	9 of 10
66794	aspartate and asparagine metabolism	88.89	8 of 9
66794	lipid A biosynthesis	88.89	8 of 9
66794	chorismate metabolism	88.89	8 of 9
66794	peptidoglycan biosynthesis	80	12 of 15
66794	glycogen metabolism	80	4 of 5
66794	Entner Doudoroff pathway	80	8 of 10
66794	tetrahydrofolate metabolism	78.57	11 of 14
66794	valine metabolism	77.78	7 of 9
66794	NAD metabolism	77.78	14 of 18
66794	serine metabolism	77.78	7 of 9
66794	CO2 fixation in Crenarchaeota	77.78	7 of 9
66794	vitamin B1 metabolism	76.92	10 of 13
66794	biotin biosynthesis	75	3 of 4
66794	CMP-KDO biosynthesis	75	3 of 4
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	ppGpp biosynthesis	75	3 of 4
66794	butanoate fermentation	75	3 of 4
66794	sulfopterin metabolism	75	3 of 4
66794	vitamin B6 metabolism	72.73	8 of 11
66794	alanine metabolism	72.41	21 of 29
66794	glutamate and glutamine metabolism	71.43	20 of 28
66794	purine metabolism	71.28	67 of 94
66794	lysine metabolism	69.05	29 of 42
66794	d-mannose degradation	66.67	6 of 9
66794	acetoin degradation	66.67	2 of 3
66794	pyrimidine metabolism	66.67	30 of 45
66794	flavin biosynthesis	66.67	10 of 15
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	(5R)-carbapenem carboxylate biosynthesis	66.67	2 of 3
66794	glycolysis	64.71	11 of 17
66794	heme metabolism	64.29	9 of 14
66794	photosynthesis	64.29	9 of 14
66794	proline metabolism	63.64	7 of 11
66794	ketogluconate metabolism	62.5	5 of 8
66794	C4 and CAM-carbon fixation	62.5	5 of 8
66794	isoleucine metabolism	62.5	5 of 8
66794	gluconeogenesis	62.5	5 of 8
66794	histidine metabolism	62.07	18 of 29
66794	propionate fermentation	60	6 of 10
66794	lipoate biosynthesis	60	3 of 5
66794	lipid metabolism	58.06	18 of 31
66794	tyrosine metabolism	57.14	8 of 14
66794	reductive acetyl coenzyme A pathway	57.14	4 of 7
66794	propanol degradation	57.14	4 of 7
66794	citric acid cycle	57.14	8 of 14
66794	glutathione metabolism	57.14	8 of 14
66794	oxidative phosphorylation	56.04	51 of 91
66794	tryptophan metabolism	55.26	21 of 38
66794	non-pathway related	55.26	21 of 38
66794	pentose phosphate pathway	54.55	6 of 11
66794	arginine metabolism	54.17	13 of 24
66794	leucine metabolism	53.85	7 of 13
66794	phosphatidylethanolamine bioynthesis	53.85	7 of 13
66794	urea cycle	53.85	7 of 13
66794	3-phenylpropionate degradation	53.33	8 of 15
66794	ethanol fermentation	50	1 of 2
66794	kanosamine biosynthesis II	50	1 of 2
66794	myo-inositol biosynthesis	50	5 of 10
66794	quinate degradation	50	1 of 2
66794	degradation of aromatic, nitrogen containing compounds	50	6 of 12
66794	cyclohexanol degradation	50	2 of 4
66794	glycogen biosynthesis	50	2 of 4
66794	cysteine metabolism	50	9 of 18
66794	phenylpropanoid biosynthesis	46.15	6 of 13
66794	polyamine pathway	43.48	10 of 23
66794	methionine metabolism	42.31	11 of 26
66794	ascorbate metabolism	40.91	9 of 22
66794	cellulose degradation	40	2 of 5
66794	bacilysin biosynthesis	40	2 of 5
66794	D-cycloserine biosynthesis	40	2 of 5
66794	glycine metabolism	40	4 of 10
66794	gallate degradation	40	2 of 5
66794	arachidonate biosynthesis	40	2 of 5
66794	factor 420 biosynthesis	40	2 of 5
66794	vitamin K metabolism	40	2 of 5
66794	methylglyoxal degradation	40	2 of 5
66794	4-hydroxyphenylacetate degradation	40	4 of 10
66794	carnitine metabolism	37.5	3 of 8
66794	androgen and estrogen metabolism	37.5	6 of 16
66794	degradation of sugar alcohols	37.5	6 of 16
66794	dTDPLrhamnose biosynthesis	37.5	3 of 8
66794	metabolism of disaccharids	36.36	4 of 11
66794	bile acid biosynthesis, neutral pathway	35.29	6 of 17
66794	phenol degradation	35	7 of 20
66794	isoprenoid biosynthesis	34.62	9 of 26
66794	enterobactin biosynthesis	33.33	1 of 3
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	cyanate degradation	33.33	1 of 3
66794	IAA biosynthesis	33.33	1 of 3
66794	glycolate and glyoxylate degradation	33.33	2 of 6
66794	pantothenate biosynthesis	33.33	2 of 6
66794	selenocysteine biosynthesis	33.33	2 of 6
66794	methane metabolism	33.33	1 of 3
66794	degradation of pentoses	28.57	8 of 28
66794	benzoyl-CoA degradation	28.57	2 of 7
66794	mevalonate metabolism	28.57	2 of 7
66794	degradation of hexoses	27.78	5 of 18
66794	arachidonic acid metabolism	27.78	5 of 18
66794	carotenoid biosynthesis	27.27	6 of 22
66794	d-xylose degradation	27.27	3 of 11
66794	lactate fermentation	25	1 of 4
66794	catecholamine biosynthesis	25	1 of 4
66794	alginate biosynthesis	25	1 of 4
66794	toluene degradation	25	1 of 4
66794	4-hydroxymandelate degradation	22.22	2 of 9
66794	nitrate assimilation	22.22	2 of 9

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Industrial	#Cooling tower
#Environmental	#Aquatic	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Isolation date
16572	water (evaporative cooling tower)	Stratford-upon-Avon	United Kingdom	GBR	Europe
50484	Water,cooling tower	Stratford-upon-Avon	United Kingdom	GBR	Europe
122579	Environment, Water, cooling tower	Stratford upon Avon	United Kingdom	GBR	Europe	1986

Taxonmaps

69479

Global distribution of 16S sequence Z49736 (>99% sequence identity) for Legionella shakespearei subclade from Microbeatlas

Aquatic Samples	34
Soil Samples	14
Animal Samples	20
Plant Samples	5
Total Samples	73

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
16572	1	Risk group (German classification) According to TRBA 466
122579	2	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM37376v1 assembly for Legionella shakespearei DSM 23087	scaffold	GCA_000373765	1122169.3	2515154162	1122169	65.04
66792	ASM146802v1 assembly for Legionella shakespearei DSM 23087 ATCC 49655	scaffold	GCA_001468025	1122169.6	2660237952	1122169	33.84

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Legionella shakespearei strain MDC1617 16S ribosomal RNA gene, partial sequence	JF720416	524		45075
16572	L.shakespearei gene for ribosomal RNA, small subunit	Z49736	1443		1122169

GC content

16572

GC-content (mol%)45.5

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM37376v1 assembly for Legionella shakespearei DSM 23087
NCBI: GCA_000373765

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	97.75	no
125439	gram_stain	BacteriaNetⓘ	negative	99.56	no
125439	motility	BacteriaNetⓘ	yes	45.58	no
125439	spore_formation	BacteriaNetⓘ	no	99.95	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Legionella shakespearei DSM 23087 ATCC 49655
NCBI: GCA_001468025.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	99.41	no
125438	anaerobic	anaerobicⓘ	no	95.72	yes
125438	aerobic	aerobicⓘ	no	69.78	no
125438	spore-forming	spore-formingⓘ	no	90.54	no
125438	thermophilic	thermophileⓘ	no	96.81	yes
125438	flagellated	motile2+ⓘ	no	54.13	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Development and evaluation of Chlamylege, a new commercial test allowing simultaneous detection and identification of Legionella, Chlamydophila pneumoniae, and Mycoplasma pneumoniae in clinical respiratory specimens by multiplex PCR.	Ginevra C, Barranger C, Ros A, Mory O, Stephan JL, Freymuth F, Joannes M, Pozzetto B, Grattard F.	J Clin Microbiol	10.1128/jcm.43.7.3247-3254.2005	2005
	Seasonal Host Shifts for Legionella Within an Industrial Water-Cooling System.	Crull S, Hammer E, Mann AE, O'Connell LM, Soule A, Griffith E, Blouin T, Brigmon RL, Richards VP.	Environ Microbiol Rep	10.1111/1758-2229.70132	2025
	In vitro Characterization of Biofilm Formation in Prevotella Species.	Albaghdadi SZ, Altaher JB, Drobiova H, Bhardwaj RG, Karched M.	Front Oral Health	10.3389/froh.2021.724194	2021
Phylogeny	Legionella spp. All Ears? The Broad Occurrence of Quorum Sensing Elements outside Legionella pneumophila.	Herran B, Greve P, Berjeaud JM, Bertaux J, Crepin A.	Genome Biol Evol	10.1093/gbe/evab032	2021
Enzymology	Evolutionary insights into Trm112-methyltransferase holoenzymes involved in translation between archaea and eukaryotes.	van Tran N, Muller L, Ross RL, Lestini R, Letoquart J, Ulryck N, Limbach PA, de Crecy-Lagard V, Cianferani S, Graille M.	Nucleic Acids Res	10.1093/nar/gky638	2018
Genetics	More than 18,000 effectors in the Legionella genus genome provide multiple, independent combinations for replication in human cells.	Gomez-Valero L, Rusniok C, Carson D, Mondino S, Perez-Cobas AE, Rolando M, Pasricha S, Reuter S, Demirtas J, Crumbach J, Descorps-Declere S, Hartland EL, Jarraud S, Dougan G, Schroeder GN, Frankel G, Buchrieser C.	Proc Natl Acad Sci U S A	10.1073/pnas.1808016116	2019
	Interaction of Legionella pneumophila and Helicobacter pylori with bacterial species isolated from drinking water biofilms.	Giao MS, Azevedo NF, Wilks SA, Vieira MJ, Keevil CW.	BMC Microbiol	10.1186/1471-2180-11-57	2011
Enzymology	Isolation of an amoeba naturally harboring a distinctive Legionella species.	Newsome AL, Scott TM, Benson RF, Fields BS.	Appl Environ Microbiol	10.1128/aem.64.5.1688-1693.1998	1998
Phylogeny	Legionella shakespearei sp. nov., isolated from cooling tower water.	Verma UK, Brenner DJ, Thacker WL, Benson RF, Vesey G, Kurtz JB, Dennis PJ, Steigerwalt AG, Robinson JS, Moss CW	Int J Syst Bacteriol	10.1099/00207713-42-3-404	1992

References

#16572	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 23087
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#34345	; Curators of the CIP;
#50484	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 31228 A
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#66793	Mukherjee et al.: GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life. 35: 676 - 683 2017 ( DOI 10.1038/nbt.3886 , PubMed 28604660 )
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#122579	Collection of Institut Pasteur ; Curators of the CIP; CIP 103541
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Legionella shakespearei (DSM 23087, ATCC 49655, CCUG 31228 A)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Multimedia

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Enzymes

Pathway annotation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000373765

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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