Strain identifier

BacDive ID: 6760

Type strain: Yes

Species: Legionella shakespearei

Strain Designation: 214

Strain history: CIP <- 2011, CCUG <- 1993, R.F. Benson, CDC, Atlanta, USA: strain CDC 214

NCBI tax ID(s): 1122169 (strain), 45075 (species)

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General

@ref: 16572

BacDive-ID: 6760

DSM-Number: 23087

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-negative

description: Legionella shakespearei 214 is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from water .

NCBI tax id

NCBI tax idMatching level
45075species
1122169strain

strain history

@refhistory
16572<- CIP <- W. L. Thacker, CDC <- U. K. Verma, Houseman Ltd., Slough, U.K.; 214
122579CIP <- 2011, CCUG <- 1993, R.F. Benson, CDC, Atlanta, USA: strain CDC 214

doi: 10.13145/bacdive6760.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Legionellales
  • family: Legionellaceae
  • genus: Legionella
  • species: Legionella shakespearei
  • full scientific name: Legionella shakespearei Verma et al. 1992

@ref: 16572

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Legionellales

family: Legionellaceae

genus: Legionella

species: Legionella shakespearei

full scientific name: Legionella shakespearei Verma et al. 1992

strain designation: 214

type strain: yes

Morphology

cell morphology

  • @ref: 69480
  • gram stain: negative
  • confidence: 99.989

colony morphology

@refincubation period
165722-3 days
504843 days

multimedia

@refmultimedia contentcaptionintellectual property rights
66793EM_DSM_23087_1.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_23087_2.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_23087_3.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_23087_4.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_23087_5.jpgelectron microscopic image© HZI/Manfred Rohde

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16572BCYE-AGAR (DSMZ Medium 585)yeshttps://mediadive.dsmz.de/medium/585Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base
34345MEDIUM 23 - for Afipia and LegionellayesDistilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml)
122579CIP Medium 23yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=23

culture temp

@refgrowthtypetemperaturerange
16572positivegrowth30mesophilic
34345positivegrowth37mesophilic
50484positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
16572microaerophile
50484aerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.977

enzymes

@refvalueactivityec
16572catalase+1.11.1.6
16572cytochrome-c oxidase-1.9.3.1

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
16572water (evaporative cooling tower)Stratford-upon-AvonUnited KingdomGBREurope
50484Water,cooling towerStratford-upon-AvonUnited KingdomGBREurope
122579Environment, Water, cooling towerStratford upon AvonUnited KingdomGBREurope1986

isolation source categories

Cat1Cat2Cat3
#Engineered#Industrial#Cooling tower
#Environmental#Aquatic

taxonmaps

  • @ref: 69479
  • File name: preview.99_886.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_35;96_527;97_608;98_699;99_886&stattab=map
  • Last taxonomy: Legionella shakespearei subclade
  • 16S sequence: Z49736
  • Sequence Identity:
  • Total samples: 73
  • soil counts: 14
  • aquatic counts: 34
  • animal counts: 20
  • plant counts: 5

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
165721Risk group (German classification)
1225792Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Legionella shakespearei strain MDC1617 16S ribosomal RNA gene, partial sequenceJF720416524ena45075
16572L.shakespearei gene for ribosomal RNA, small subunitZ497361443ena1122169

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Legionella shakespearei DSM 23087 ATCC 49655GCA_001468025scaffoldncbi1122169
66792Legionella shakespearei DSM 23087GCA_000373765scaffoldncbi1122169
66792Legionella shakespearei DSM 230871122169.6wgspatric1122169
66792Legionella shakespearei DSM 230871122169.3wgspatric1122169
66792Legionella shakespearei DSM 230872515154162draftimg1122169
66792Legionella shakespearei ATCC 496552660237952draftimg1122169

GC content

  • @ref: 16572
  • GC-content: 45.5

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedno79.469no
gram-positiveno97.768no
anaerobicno99.009no
halophileno81.605no
spore-formingno94.346no
glucose-utilyes61.729no
motileyes81.12no
aerobicno85.187no
thermophileno98.517yes
glucose-fermentno88.825no

External links

@ref: 16572

culture collection no.: DSM 23087, ATCC 49655, CCUG 31228 A, CIP 103541, NCTC 12829, CDC 214

straininfo link

  • @ref: 76230
  • straininfo: 42605

literature

  • topic: Phylogeny
  • Pubmed-ID: 1503972
  • title: Legionella shakespearei sp. nov., isolated from cooling tower water.
  • authors: Verma UK, Brenner DJ, Thacker WL, Benson RF, Vesey G, Kurtz JB, Dennis PJ, Steigerwalt AG, Robinson JS, Moss CW
  • journal: Int J Syst Bacteriol
  • DOI: 10.1099/00207713-42-3-404
  • year: 1992
  • mesh: Base Composition, DNA, Bacterial/chemistry, Fatty Acids/analysis, Legionella/chemistry/*classification/isolation & purification/physiology, Ubiquinone/analysis, *Water Microbiology
  • topic2: Enzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
16572Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23087)https://www.dsmz.de/collection/catalogue/details/culture/DSM-23087
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
34345Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15350
50484Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 31228 A)https://www.ccug.se/strain?id=31228
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66793Mukherjee et al.10.1038/nbt.3886GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life 35: 676-683 201728604660
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
76230Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID42605.1StrainInfo: A central database for resolving microbial strain identifiers
122579Curators of the CIPCollection of Institut Pasteur (CIP 103541)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103541