Name: Legionella feeleii WO-44C
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 6734

Type strain:

Species: Legionella feeleii

Strain Designation: WO-44C

Culture col. no.: DSM 17645, ATCC 35072, CCUG 16417, CIP 103877, NCTC 12022

Strain history: CIP <- 1993, C. Tram, Inst. Pasteur, Paris, France <- ATCC <- G.W. Gorman: strain WO-44C

NCBI tax ID(s): 453 (species)

SI-ID 40852

ATCC 35072, NCTC 12022, CCUG 16417, CIP 103877, DSM 17645

Special Collections

Other strains from Legionella feeleii

Section

Legionella feeleii WO-44C is a microaerophile, Gram-negative bacterium that was isolated from grinding machine coolant fluid.

Gram-negative
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Legionellales*
	Family	*Legionellaceae*
	Genus	*Legionella*
	Species	**Legionella feeleii**
	Full Scientific Name (LPSN)	Legionella feeleii Herwaldt et al. 1984

Information on morphological and physiological properties Morphology

[Ref.: #119295]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %98.894

[Ref.: #119295]	Cell shape	rod-shaped

[Ref.: #119295]	Motility	no

[Ref.: #7107]

Incubation period

2-3 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7107]

Culture medium

BCYE-AGAR (DSMZ Medium 585)

[Ref.: #7107]

Culture medium growth

[Ref.: #7107]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7107]

Culture medium composition

expand / minimize

[Ref.: #40844]

Culture medium

MEDIUM 23 - for Afipia and Legionella

[Ref.: #40844]

Culture medium growth

[Ref.: #40844]

Culture medium composition

expand / minimize

[Ref.: #119295]

Culture medium

CIP Medium 23

[Ref.: #119295]

Culture medium growth

[Ref.: #119295]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #7107]	growth	37 °C
[Ref.: #40844]	growth	37 °C
[Ref.: #46480]	growth	37 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #7107]	Oxygen tolerance	microaerophile
[Ref.: #46480]	Oxygen tolerance	microaerophile

[Ref.: #69481]

Ability of spore formation

no Confidence in %100

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #119295]		nitrate ChEBI	-	reduction
[Ref.: #119295]		nitrite ChEBI	-	reduction

	Metabolite production	Metabolite	Production
[Ref.: #119295]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #7107]	catalase	+	1.11.1.6
[Ref.: #119295]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #7107]	cytochrome-c oxidase	-	1.9.3.1
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #119295]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #119295]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #7107]	13996	-	+	+/-	+/-	-	+	-	-	-	-	+/-	+/-	-	-	-	-	-	-	-	-
[Ref.: #119295]	7720	-	+	-	-	-	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-

	Pathway	Enzyme coverage	Annotated reactions
	Pathway annotation	Computationally determined by
[Ref.: #66794]	anapleurotic synthesis of oxalacetate	100	1 of 1
[Ref.: #66794]	biotin biosynthesis	100	4 of 4
[Ref.: #66794]	acetoin degradation	100	3 of 3
[Ref.: #66794]	adipate degradation	100	2 of 2
[Ref.: #66794]	palmitate biosynthesis	100	22 of 22
[Ref.: #66794]	cis-vaccenate biosynthesis	100	2 of 2
[Ref.: #66794]	cardiolipin biosynthesis	100	7 of 7
[Ref.: #66794]	formaldehyde oxidation	100	3 of 3
[Ref.: #66794]	ubiquinone biosynthesis	100	7 of 7
[Ref.: #66794]	coenzyme A metabolism	100	4 of 4
[Ref.: #66794]	sulfopterin metabolism	100	4 of 4
[Ref.: #66794]	CDP-diacylglycerol biosynthesis	100	2 of 2
[Ref.: #66794]	suberin monomers biosynthesis	100	2 of 2
[Ref.: #66794]	UDP-GlcNAc biosynthesis	100	3 of 3
[Ref.: #66794]	folate polyglutamylation	100	1 of 1
[Ref.: #66794]	lipid A biosynthesis	88.89	8 of 9
[Ref.: #66794]	CO2 fixation in Crenarchaeota	88.89	8 of 9
[Ref.: #66794]	d-mannose degradation	88.89	8 of 9
[Ref.: #66794]	chorismate metabolism	88.89	8 of 9
[Ref.: #66794]	NAD metabolism	83.33	15 of 18
[Ref.: #66794]	proline metabolism	81.82	9 of 11
[Ref.: #66794]	vitamin B6 metabolism	81.82	9 of 11
[Ref.: #66794]	peptidoglycan biosynthesis	80	12 of 15
[Ref.: #66794]	Entner Doudoroff pathway	80	8 of 10
[Ref.: #66794]	hydrogen production	80	4 of 5
[Ref.: #66794]	tetrahydrofolate metabolism	78.57	11 of 14
[Ref.: #66794]	valine metabolism	77.78	7 of 9
[Ref.: #66794]	serine metabolism	77.78	7 of 9
[Ref.: #66794]	vitamin B1 metabolism	76.92	10 of 13
[Ref.: #66794]	leucine metabolism	76.92	10 of 13
[Ref.: #66794]	phenylalanine metabolism	76.92	10 of 13
[Ref.: #66794]	glycolysis	76.47	13 of 17
[Ref.: #66794]	alanine metabolism	75.86	22 of 29
[Ref.: #66794]	butanoate fermentation	75	3 of 4
[Ref.: #66794]	CMP-KDO biosynthesis	75	3 of 4
[Ref.: #66794]	ppGpp biosynthesis	75	3 of 4
[Ref.: #66794]	acetate fermentation	75	3 of 4
[Ref.: #66794]	lactate fermentation	75	3 of 4
[Ref.: #66794]	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
[Ref.: #66794]	lipid metabolism	74.19	23 of 31
[Ref.: #66794]	purine metabolism	73.4	69 of 94
[Ref.: #66794]	flavin biosynthesis	73.33	11 of 15
[Ref.: #66794]	photosynthesis	71.43	10 of 14
[Ref.: #66794]	reductive acetyl coenzyme A pathway	71.43	5 of 7
[Ref.: #66794]	pyrimidine metabolism	71.11	32 of 45
[Ref.: #66794]	threonine metabolism	70	7 of 10
[Ref.: #66794]	propionate fermentation	70	7 of 10
[Ref.: #66794]	lysine metabolism	69.05	29 of 42
[Ref.: #66794]	glutamate and glutamine metabolism	67.86	19 of 28
[Ref.: #66794]	octane oxidation	66.67	2 of 3
[Ref.: #66794]	cyanate degradation	66.67	2 of 3
[Ref.: #66794]	L-lactaldehyde degradation	66.67	2 of 3
[Ref.: #66794]	IAA biosynthesis	66.67	2 of 3
[Ref.: #66794]	aspartate and asparagine metabolism	66.67	6 of 9
[Ref.: #66794]	acetyl CoA biosynthesis	66.67	2 of 3
[Ref.: #66794]	heme metabolism	64.29	9 of 14
[Ref.: #66794]	ketogluconate metabolism	62.5	5 of 8
[Ref.: #66794]	gluconeogenesis	62.5	5 of 8
[Ref.: #66794]	C4 and CAM-carbon fixation	62.5	5 of 8
[Ref.: #66794]	isoleucine metabolism	62.5	5 of 8
[Ref.: #66794]	histidine metabolism	62.07	18 of 29
[Ref.: #66794]	phosphatidylethanolamine bioynthesis	61.54	8 of 13
[Ref.: #66794]	lipoate biosynthesis	60	3 of 5
[Ref.: #66794]	methylglyoxal degradation	60	3 of 5
[Ref.: #66794]	cellulose degradation	60	3 of 5
[Ref.: #66794]	non-pathway related	57.89	22 of 38
[Ref.: #66794]	propanol degradation	57.14	4 of 7
[Ref.: #66794]	citric acid cycle	57.14	8 of 14
[Ref.: #66794]	glutathione metabolism	57.14	8 of 14
[Ref.: #66794]	ascorbate metabolism	54.55	12 of 22
[Ref.: #66794]	3-phenylpropionate degradation	53.33	8 of 15
[Ref.: #66794]	tryptophan metabolism	50	19 of 38
[Ref.: #66794]	degradation of aromatic, nitrogen containing compounds	50	6 of 12
[Ref.: #66794]	kanosamine biosynthesis II	50	1 of 2
[Ref.: #66794]	arginine metabolism	50	12 of 24
[Ref.: #66794]	pantothenate biosynthesis	50	3 of 6
[Ref.: #66794]	carnitine metabolism	50	4 of 8
[Ref.: #66794]	quinate degradation	50	1 of 2
[Ref.: #66794]	glycogen biosynthesis	50	2 of 4
[Ref.: #66794]	ethanol fermentation	50	1 of 2
[Ref.: #66794]	oxidative phosphorylation	48.35	44 of 91
[Ref.: #66794]	phenylpropanoid biosynthesis	46.15	6 of 13
[Ref.: #66794]	pentose phosphate pathway	45.45	5 of 11
[Ref.: #66794]	cysteine metabolism	44.44	8 of 18
[Ref.: #66794]	tyrosine metabolism	42.86	6 of 14
[Ref.: #66794]	methionine metabolism	42.31	11 of 26
[Ref.: #66794]	gallate degradation	40	2 of 5
[Ref.: #66794]	myo-inositol biosynthesis	40	4 of 10
[Ref.: #66794]	glycine metabolism	40	4 of 10
[Ref.: #66794]	3-chlorocatechol degradation	40	2 of 5
[Ref.: #66794]	arachidonate biosynthesis	40	2 of 5
[Ref.: #66794]	polyamine pathway	39.13	9 of 23
[Ref.: #66794]	urea cycle	38.46	5 of 13
[Ref.: #66794]	isoprenoid biosynthesis	38.46	10 of 26
[Ref.: #66794]	dTDPLrhamnose biosynthesis	37.5	3 of 8
[Ref.: #66794]	degradation of sugar alcohols	37.5	6 of 16
[Ref.: #66794]	androgen and estrogen metabolism	37.5	6 of 16
[Ref.: #66794]	cholesterol biosynthesis	36.36	4 of 11
[Ref.: #66794]	degradation of pentoses	35.71	10 of 28
[Ref.: #66794]	glycolate and glyoxylate degradation	33.33	2 of 6
[Ref.: #66794]	sulfoquinovose degradation	33.33	1 of 3
[Ref.: #66794]	methane metabolism	33.33	1 of 3
[Ref.: #66794]	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
[Ref.: #66794]	sphingosine metabolism	33.33	2 of 6
[Ref.: #66794]	enterobactin biosynthesis	33.33	1 of 3
[Ref.: #66794]	arachidonic acid metabolism	33.33	6 of 18
[Ref.: #66794]	selenocysteine biosynthesis	33.33	2 of 6
[Ref.: #66794]	4-hydroxyphenylacetate degradation	30	3 of 10
[Ref.: #66794]	mevalonate metabolism	28.57	2 of 7
[Ref.: #66794]	aclacinomycin biosynthesis	28.57	2 of 7
[Ref.: #66794]	degradation of hexoses	27.78	5 of 18
[Ref.: #66794]	carotenoid biosynthesis	27.27	6 of 22
[Ref.: #66794]	d-xylose degradation	27.27	3 of 11
[Ref.: #66794]	catecholamine biosynthesis	25	1 of 4
[Ref.: #66794]	cyclohexanol degradation	25	1 of 4
[Ref.: #66794]	vitamin E metabolism	25	1 of 4
[Ref.: #66794]	phenol degradation	25	5 of 20
[Ref.: #66794]	toluene degradation	25	1 of 4
[Ref.: #66794]	bile acid biosynthesis, neutral pathway	23.53	4 of 17
[Ref.: #66794]	sulfate reduction	23.08	3 of 13
[Ref.: #66794]	nitrate assimilation	22.22	2 of 9
		Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7107]	Sample type/isolated from	grinding machine coolant fluid
[Ref.: #7107]	Country	USA
[Ref.: #7107]	Country ISO 3 Code	USA
[Ref.: #7107]	Continent	North America

[Ref.: #46480]	Sample type/isolated from	Grinding machine coolant fluid

[Ref.: #119295]	Sample type/isolated from	Grinding machine coolant fluid
* marker position based on {}

Isolation sources categories

#Engineered	#Industrial	#Industrial production
#Engineered	#Industrial	#Machines and devices

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7107]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #119295]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #7107]	L.feeleii (ATCC 35072) gene for 16S rRNA	X73395	1466	GenBank ENA	453 tax ID
[Ref.: #20218]	Legionella feeleii strain MDC1579 16S ribosomal RNA gene, partial sequence	JF720366	524	GenBank ENA	453 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Legionella feeleii WO-44C	2695420407	draft	JGI IMG/M	453 tax ID	*
[Ref.: #66792]	Legionella feeleii ATCC 35072	GCA_001648615	scaffold	GenBank ENA	453 tax ID	*
[Ref.: #66792]	Legionella feeleii WO-44C	GCA_001467625	scaffold	GenBank ENA	453 tax ID	*
[Ref.: #66792]	Legionella feeleii WO-44C	GCA_900639755	scaffold	GenBank ENA	453 tax ID	*
[Ref.: #66792]	Legionella feeleii strain ATCC 35072	453.5	wgs	PATRIC	453 tax ID	*
[Ref.: #66792]	Legionella feeleii strain NCTC12022	453.9	wgs	PATRIC	453 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Legionella feeleii WO-44C NCBI: GCA_900639755.1
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Legionella feeleii ATCC 35072 NCBI: GCA_001648615.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	98.894	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	95.645	yes
[Ref.: #69480]	aerobic	aerobicⓘ	no	77.987	yes
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	86.584	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	96.438	no
[Ref.: #69480]	flagellated	motile2+ⓘ	yes	51.643	no

Availability in culture collections External links

[Ref.: #7107]

Culture collection no.

DSM 17645, ATCC 35072, CCUG 16417, CIP 103877, NCTC 12022

[Ref.: #76204]

SI-ID 40852

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Pathogenicity	Legionella feeleii: pneumonia or Pontiac fever? Bacterial virulence traits and host immune response.	Wang C, Chuai X, Liang M	Med Microbiol Immunol	10.1007/s00430-018-0571-0	2018	*
Pathogenicity	Colony types and virulence traits of Legionella feeleii determined by exopolysaccharide materials.	Wang C, Saito M, Ogawa M, Yoshida S	FEMS Microbiol Lett	10.1093/femsle/fnw098	2016	*
Cultivation	Comparative analysis of virulence traits between a Legionella feeleii strain implicated in Pontiac fever and a strain that caused Legionnaires' disease.	Wang C, Saito M, Tanaka T, Amako K, Yoshida S	Microb Pathog	10.1016/j.micpath.2015.09.004	2015	*
Phylogeny	Legionella tunisiensis sp. nov. and Legionella massiliensis sp. nov., isolated from environmental water samples.	Campocasso A, Boughalmi M, Fournous G, Raoult D, La Scola B	Int J Syst Evol Microbiol	10.1099/ijs.0.037853-0	2012	*
Pathogenicity	Morphological variety of intracellular microcolonies of Legionella species in Vero cells.	Ogawa M, Takade A, Miyamoto H, Taniguchi H, Yoshida S	Microbiol Immunol	10.1111/j.1348-0421.2001.tb02658.x	2001	*
Phylogeny	Second serogroup of Legionella feeleii strains isolated from humans.	Thacker WL, Wilkinson HW, Plikaytis BB, Steigerwalt AG, Mayberry WR, Moss CW, Brenner DJ	J Clin Microbiol	10.1128/jcm.22.1.1-4.1985	1985	*
Pathogenicity	A new Legionella species, Legionella feeleii species nova, causes Pontiac fever in an automobile plant.	Herwaldt LA, Gorman GW, McGrath T, Toma S, Brake B, Hightower AW, Jones J, Reingold AL, Boxer PA, Tang PW, et al.	Ann Intern Med	10.7326/0003-4819-100-3-333	1984	*

References

#7107

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 17645

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#40844 ; Curators of the CIP;
#46480 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 16417

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66794

Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#76204

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID40852.1 )

#119295 Collection of Institut Pasteur ; Curators of the CIP; CIP 103877
* These data were automatically processed and therefore are not curated

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Legionella feeleii WO-44C

Legionella feeleii ATCC 35072

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