Strain identifier

BacDive ID: 6734

Type strain: Yes

Species: Legionella feeleii

Strain Designation: WO-44C

Strain history: CIP <- 1993, C. Tram, Inst. Pasteur, Paris, France <- ATCC <- G.W. Gorman: strain WO-44C

NCBI tax ID(s): 453 (species)

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General

@ref: 7107

BacDive-ID: 6734

DSM-Number: 17645

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-negative

description: Legionella feeleii WO-44C is a microaerophile, mesophilic, Gram-negative bacterium that was isolated from grinding machine coolant fluid.

NCBI tax id

  • NCBI tax id: 453
  • Matching level: species

strain history

@refhistory
7107<- H. E. Müller, Medizinal-Untersuchungsamt, Braunschweig <- ATCC <- G. W. Gorman, CDC; WO-44C
119295CIP <- 1993, C. Tram, Inst. Pasteur, Paris, France <- ATCC <- G.W. Gorman: strain WO-44C

doi: 10.13145/bacdive6734.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Legionellales
  • family: Legionellaceae
  • genus: Legionella
  • species: Legionella feeleii
  • full scientific name: Legionella feeleii Herwaldt et al. 1984

@ref: 7107

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Legionellales

family: Legionellaceae

genus: Legionella

species: Legionella feeleii

full scientific name: Legionella feeleii Herwaldt et al. 1984

strain designation: WO-44C

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480negative99.999
119295negativerod-shapedno

colony morphology

  • @ref: 7107
  • incubation period: 2-3 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7107BCYE-AGAR (DSMZ Medium 585)yeshttps://mediadive.dsmz.de/medium/585Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base
40844MEDIUM 23 - for Afipia and LegionellayesDistilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml)
119295CIP Medium 23yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=23

culture temp

@refgrowthtypetemperaturerange
7107positivegrowth37mesophilic
40844positivegrowth37mesophilic
46480positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
7107microaerophile
46480microaerophile

spore formation

@refspore formationconfidence
69481no100
69480no99.998

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11929517632nitrate-reduction
11929516301nitrite-reduction

metabolite production

  • @ref: 119295
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
7107catalase+1.11.1.6
7107cytochrome-c oxidase-1.9.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382valine arylamidase-
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382alkaline phosphatase+3.1.3.1
119295oxidase-
119295catalase+1.11.1.6
119295urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
7107-++/-+/--+----+/-+/---------
119295-+---+----++--------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
7107grinding machine coolant fluidUSAUSANorth America
46480Grinding machine coolant fluid
119295Grinding machine coolant fluid

isolation source categories

Cat1Cat2Cat3
#Engineered#Industrial#Industrial production
#Engineered#Industrial#Machines and devices

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
71072Risk group (German classification)
1192952Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Legionella feeleii strain MDC1579 16S ribosomal RNA gene, partial sequenceJF720366524ena453
7107L.feeleii (ATCC 35072) gene for 16S rRNAX733951466ena453

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Legionella feeleii NCTC12022GCA_900452475contigncbi453
66792Legionella feeleii ATCC 35072GCA_001648615scaffoldncbi453
66792Legionella feeleii WO-44CGCA_001467625scaffoldncbi453
66792Legionella feeleii WO-44CGCA_900639755scaffoldncbi453
66792Legionella feeleii strain ATCC 35072453.5wgspatric453
66792Legionella feeleii strain NCTC12022453.9wgspatric453
66792Legionella feeleii strain WO-44C453.4wgspatric453
66792Legionella feeleii NCTC 120222808606639draftimg453
66792Legionella feeleii WO-44C2695420407draftimg453

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedno73.495no
gram-positiveno98.608no
anaerobicno97.78yes
halophileno87.232no
spore-formingno92.957no
glucose-utilyes61.471no
motileyes85.984no
aerobicno92.548yes
thermophileno96.741yes
glucose-fermentno87.288no

External links

@ref: 7107

culture collection no.: DSM 17645, ATCC 35072, CCUG 16417, CIP 103877, NCTC 12022

straininfo link

  • @ref: 76204
  • straininfo: 40852

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny3894411Second serogroup of Legionella feeleii strains isolated from humans.Thacker WL, Wilkinson HW, Plikaytis BB, Steigerwalt AG, Mayberry WR, Moss CW, Brenner DJJ Clin Microbiol10.1128/jcm.22.1.1-4.19851985Animals, Antigens, Bacterial/immunology, Chick Embryo, DNA, Bacterial, Fatty Acids/analysis, Fluorescent Antibody Technique, Humans, Legionella/analysis/*classification/immunology/isolation & purification, Legionnaires' Disease/*microbiology, Nucleic Acid Hybridization, Pneumonia/*microbiology, Rabbits, Serotyping, Ubiquinone/analysisPathogenicity
Pathogenicity6696354A new Legionella species, Legionella feeleii species nova, causes Pontiac fever in an automobile plant.Herwaldt LA, Gorman GW, McGrath T, Toma S, Brake B, Hightower AW, Jones J, Reingold AL, Boxer PA, Tang PW, et al.Ann Intern Med10.7326/0003-4819-100-3-3331984Aerosols, Air Microbiology, Automobiles, Bacterial Infections/epidemiology/*microbiology, Disease Outbreaks/*epidemiology, Female, Humans, Legionella/classification/*isolation & purification, Male, Occupational Diseases/epidemiology/*microbiology, Ontario, Risk, Serologic Tests, Surveys and QuestionnairesPhylogeny
Pathogenicity11529563Morphological variety of intracellular microcolonies of Legionella species in Vero cells.Ogawa M, Takade A, Miyamoto H, Taniguchi H, Yoshida SMicrobiol Immunol10.1111/j.1348-0421.2001.tb02658.x2001Animals, Chlorocebus aethiops, Humans, Legionella/*classification/pathogenicity/physiology/*ultrastructure, Microscopy, Electron, Vero CellsPhylogeny
Phylogeny22307511Legionella tunisiensis sp. nov. and Legionella massiliensis sp. nov., isolated from environmental water samples.Campocasso A, Boughalmi M, Fournous G, Raoult D, La Scola BInt J Syst Evol Microbiol10.1099/ijs.0.037853-02012Amoeba/growth & development, Bacterial Typing Techniques, DNA, Bacterial/genetics, France, Genes, Bacterial, Legionella/*classification/genetics/*isolation & purification, Molecular Sequence Data, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Tunisia, *Water MicrobiologyGenetics
Cultivation26386398Comparative analysis of virulence traits between a Legionella feeleii strain implicated in Pontiac fever and a strain that caused Legionnaires' disease.Wang C, Saito M, Tanaka T, Amako K, Yoshida SMicrob Pathog10.1016/j.micpath.2015.09.0042015Animals, Bacterial Load, Bacteriological Techniques, Cell Line, Culture Media, Cytokines/metabolism, Epithelial Cells/immunology/microbiology, *Genetic Variation, Humans, Legionella/*genetics/growth & development/*isolation & purification/physiology, Legionellosis/*microbiology/*pathology, Locomotion, Macrophages/immunology/microbiology, Mice, Temperature, Virulence, Virulence Factors/*geneticsPathogenicity
Pathogenicity27190244Colony types and virulence traits of Legionella feeleii determined by exopolysaccharide materials.Wang C, Saito M, Ogawa M, Yoshida SFEMS Microbiol Lett10.1093/femsle/fnw0982016Animals, Guinea Pigs, HeLa Cells, Humans, Legionella/*cytology/growth & development/metabolism/*pathogenicity, Legionellosis/*microbiology, Macrophages/microbiology, Mice, Microscopy, Electron, Phagocytosis, Phenotype, Polysaccharides, Bacterial/*analysis/chemistry/metabolism, Ruthenium Red, U937 Cells, VirulenceMetabolism
Pathogenicity30386929Legionella feeleii: pneumonia or Pontiac fever? Bacterial virulence traits and host immune response.Wang C, Chuai X, Liang MMed Microbiol Immunol10.1007/s00430-018-0571-02018Animals, Cell Line, Cytokines/*metabolism, Humans, Legionella/growth & development/*pathogenicity, Legionnaires' Disease/*microbiology/*pathology, Mice, Polysaccharides, Bacterial/*metabolism/toxicity, Virulence, Virulence Factors/*metabolism/toxicityMetabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
7107Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 17645)https://www.dsmz.de/collection/catalogue/details/culture/DSM-17645
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40844Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15723
46480Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 16417)https://www.ccug.se/strain?id=16417
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
76204Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID40852.1StrainInfo: A central database for resolving microbial strain identifiers
119295Curators of the CIPCollection of Institut Pasteur (CIP 103877)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103877