Legionella rubrilucens (CCUG 29671, DSM 11884, ATCC 35304)

Name: Legionella rubrilucens (CCUG 29671, DSM 11884, ATCC 35304)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 6731

Type strain

Strain Designation WA-270A-C2

Culture col. no. CCUG 29671 DSM 11884 ATCC 35304 NCTC 11987 JCM 7565 2 more

NCBI tax ID(s) 458

Special Collections DSMZ CCUG JCM CIP

History DSMZ <- H. E. Müller <- ATCC <- G. W. Gorman JCM <-- GIFU 10743 <-- CDC WA-270A-C2. CIP <- 1993, R.F. Benson, CDC: strain WA-270A-C2 <- G.W. Gormann

Links

Legionella rubrilucens WA-270A-C2 is a microaerophile, Gram-negative, rod-shaped bacterium of the family Legionellaceae.

Gram-negative rod-shaped microaerophile genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 40984

ATCC 35304,NCTC 11987,CCUG 29671,CIP 103848,JCM 7565,DSM 11884

@ref 20215

Last LPSN update 2026-05-29 08:26:28

Domain Bacteria

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Legionellales

Family Legionellaceae

Genus Legionella

Species Legionella rubrilucens

Full scientific name Legionella rubrilucens Brenner et al. 1985

BacDive ID	Other strains from **Legionella rubrilucens** (1)	Type strain
162118	L. rubrilucens JCM 17837

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
122290	negative	rod-shaped
125438	negative		98.494
125439	negative		97.606

Colony morphology

4522

Incubation period1-2 days

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
4522	BCYE-AGAR (DSMZ Medium 585)	Medium recipe at MediaDive	Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base
34426	MEDIUM 23 - for Afipia and Legionella		Distilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml)
122290	CIP Medium 23	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
4522	positive	growth	37
34426	positive	growth	37
67770	positive	growth	37

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
4522	microaerophile
125439	facultative anaerobe	90.384

Spore formation

@ref	Spore formation	Confidence
125439		99.201

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
122290	17632 ChEBI	nitrate	-	reduction
122290	16301 ChEBI	nitrite	-	reduction

Metabolite production

@ref	Chebi-ID	Metabolite	Production
122290	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
4522	catalase	+	1.11.1.6
122290	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
4522	cytochrome-c oxidase	-	1.9.3.1
68382	esterase lipase (C 8)	+		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
122290	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
122290	urease	-	3.5.1.5

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	biotin biosynthesis	100	4 of 4
66794	coenzyme A metabolism	100	4 of 4
66794	formaldehyde oxidation	100	3 of 3
66794	acetate fermentation	100	4 of 4
66794	adipate degradation	100	2 of 2
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	suberin monomers biosynthesis	100	2 of 2
66794	ubiquinone biosynthesis	100	7 of 7
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	cardiolipin biosynthesis	100	7 of 7
66794	octane oxidation	100	3 of 3
66794	folate polyglutamylation	100	1 of 1
66794	ppGpp biosynthesis	100	4 of 4
66794	palmitate biosynthesis	100	22 of 22
66794	lipid A biosynthesis	88.89	8 of 9
66794	chorismate metabolism	88.89	8 of 9
66794	aspartate and asparagine metabolism	88.89	8 of 9
66794	valine metabolism	88.89	8 of 9
66794	serine metabolism	88.89	8 of 9
66794	NAD metabolism	83.33	15 of 18
66794	vitamin B6 metabolism	81.82	9 of 11
66794	Entner Doudoroff pathway	80	8 of 10
66794	hydrogen production	80	4 of 5
66794	myo-inositol biosynthesis	80	8 of 10
66794	glycogen metabolism	80	4 of 5
66794	peptidoglycan biosynthesis	80	12 of 15
66794	threonine metabolism	80	8 of 10
66794	tetrahydrofolate metabolism	78.57	11 of 14
66794	CO2 fixation in Crenarchaeota	77.78	7 of 9
66794	vitamin B1 metabolism	76.92	10 of 13
66794	phenylalanine metabolism	76.92	10 of 13
66794	leucine metabolism	76.92	10 of 13
66794	purine metabolism	76.6	72 of 94
66794	pyrimidine metabolism	75.56	34 of 45
66794	CMP-KDO biosynthesis	75	3 of 4
66794	sulfopterin metabolism	75	3 of 4
66794	butanoate fermentation	75	3 of 4
66794	flavin biosynthesis	73.33	11 of 15
66794	alanine metabolism	72.41	21 of 29
66794	reductive acetyl coenzyme A pathway	71.43	5 of 7
66794	glutamate and glutamine metabolism	67.86	19 of 28
66794	enterobactin biosynthesis	66.67	2 of 3
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	acetoin degradation	66.67	2 of 3
66794	d-mannose degradation	66.67	6 of 9
66794	(5R)-carbapenem carboxylate biosynthesis	66.67	2 of 3
66794	histidine metabolism	65.52	19 of 29
66794	glycolysis	64.71	11 of 17
66794	photosynthesis	64.29	9 of 14
66794	heme metabolism	64.29	9 of 14
66794	lysine metabolism	64.29	27 of 42
66794	proline metabolism	63.64	7 of 11
66794	tryptophan metabolism	63.16	24 of 38
66794	gluconeogenesis	62.5	5 of 8
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	62.5	5 of 8
66794	C4 and CAM-carbon fixation	62.5	5 of 8
66794	isoleucine metabolism	62.5	5 of 8
66794	ketogluconate metabolism	62.5	5 of 8
66794	lipid metabolism	61.29	19 of 31
66794	non-pathway related	60.53	23 of 38
66794	3-phenylpropionate degradation	60	9 of 15
66794	propionate fermentation	60	6 of 10
66794	methylglyoxal degradation	60	3 of 5
66794	lipoate biosynthesis	60	3 of 5
66794	cellulose degradation	60	3 of 5
66794	citric acid cycle	57.14	8 of 14
66794	glutathione metabolism	57.14	8 of 14
66794	tyrosine metabolism	57.14	8 of 14
66794	propanol degradation	57.14	4 of 7
66794	degradation of sugar alcohols	56.25	9 of 16
66794	pentose phosphate pathway	54.55	6 of 11
66794	polyamine pathway	52.17	12 of 23
66794	degradation of aromatic, nitrogen containing compounds	50	6 of 12
66794	ethanol fermentation	50	1 of 2
66794	kanosamine biosynthesis II	50	1 of 2
66794	quinate degradation	50	1 of 2
66794	pantothenate biosynthesis	50	3 of 6
66794	glycine metabolism	50	5 of 10
66794	phenylmercury acetate degradation	50	1 of 2
66794	methionine metabolism	50	13 of 26
66794	arginine metabolism	45.83	11 of 24
66794	oxidative phosphorylation	43.96	40 of 91
66794	mevalonate metabolism	42.86	3 of 7
66794	ascorbate metabolism	40.91	9 of 22
66794	glycine betaine biosynthesis	40	2 of 5
66794	arachidonate biosynthesis	40	2 of 5
66794	cysteine metabolism	38.89	7 of 18
66794	phosphatidylethanolamine bioynthesis	38.46	5 of 13
66794	phenylpropanoid biosynthesis	38.46	5 of 13
66794	androgen and estrogen metabolism	37.5	6 of 16
66794	cholesterol biosynthesis	36.36	4 of 11
66794	isoprenoid biosynthesis	34.62	9 of 26
66794	4-hydroxymandelate degradation	33.33	3 of 9
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	IAA biosynthesis	33.33	1 of 3
66794	selenocysteine biosynthesis	33.33	2 of 6
66794	arachidonic acid metabolism	33.33	6 of 18
66794	methane metabolism	33.33	1 of 3
66794	glycolate and glyoxylate degradation	33.33	2 of 6
66794	cyanate degradation	33.33	1 of 3
66794	urea cycle	30.77	4 of 13
66794	degradation of pentoses	28.57	8 of 28
66794	benzoyl-CoA degradation	28.57	2 of 7
66794	degradation of hexoses	27.78	5 of 18
66794	d-xylose degradation	27.27	3 of 11
66794	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
66794	metabolism of disaccharids	27.27	3 of 11
66794	cyclohexanol degradation	25	1 of 4
66794	toluene degradation	25	1 of 4
66794	lactate fermentation	25	1 of 4
66794	dTDPLrhamnose biosynthesis	25	2 of 8
66794	catecholamine biosynthesis	25	1 of 4
66794	glycogen biosynthesis	25	1 of 4
66794	phenol degradation	25	5 of 20
66794	alginate biosynthesis	25	1 of 4
66794	sulfate reduction	23.08	3 of 13
66794	carotenoid biosynthesis	22.73	5 of 22
66794	nitrate assimilation	22.22	2 of 9

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
122290	-	+	+	+	-	+	+	-	-	-	+	+	-	-	-	-	-	-	-	-
4522	-	+	-	+	-	+	+/-	-	-	-	+	+	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
4522			USA	USA	North America
67770	Tap water	Los Angeles, CA	USA	USA	North America
122290	Other, Tap water	Los Angeles, California	United States of America	USA	North America

Taxonmaps

69479

Global distribution of 16S sequence Z32643 (>99% sequence identity) for Legionella from Microbeatlas

Aquatic Samples	438
Soil Samples	154
Animal Samples	55
Plant Samples	35
Total Samples	682

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
4522	1	Risk group (German classification) According to TRBA 466
122290	2	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
124043	ASM146812v1 assembly for Legionella rubrilucens WA-270A-C2	scaffold	GCA_001468125	458.5	2660238232	458	70.4

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Legionella rubrilucens strain MDC1576 16S ribosomal RNA gene, partial sequence	JF720409	525	458
20218	L.rubrilucens (WA-270A-C2) gene for 16S ribosomal RNA	Z32643	1456	458
4522	L.rubrilucens (ATCC 35304) gene for 16S rRNA	X73398	1468	458

GC content

@ref	GC-content (mol%)	Method
67770	52	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM146812v1 assembly for Legionella rubrilucens WA-270A-C2
NCBI: GCA_001468125

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	90.38	no
125439	gram_stain	BacteriaNetⓘ	negative	97.61	no
125439	motility	BacteriaNetⓘ	yes	63.09	no
125439	spore_formation	BacteriaNetⓘ	no	99.20	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Legionella rubrilucens strain WA-270A-C2
PATRIC: 458.5

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	98.49	no
125438	anaerobic	anaerobicⓘ	no	96.99	no
125438	spore-forming	spore-formingⓘ	no	88.05	no
125438	aerobic	aerobicⓘ	no	79.16	yes
125438	thermophilic	thermophileⓘ	no	95.99	no
125438	flagellated	motile2+ⓘ	no	64.30	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	New Insight regarding Legionella Non-Pneumophila Species Identification: Comparison between the Traditional mip Gene Classification Scheme and a Newly Proposed Scheme Targeting the rpoB Gene.	Pascale MR, Salaris S, Mazzotta M, Girolamini L, Fregni Serpini G, Manni L, Grottola A, Cristino S.	Microbiol Spectr	10.1128/spectrum.01161-21	2021
	Rapid Detection and Differentiation of Legionella pneumophila and Non-Legionella pneumophila Species by Using Recombinase Polymerase Amplification Combined With EuNPs-Based Lateral Flow Immunochromatography.	Du J, Ma B, Li J, Wang Y, Dou T, Xu S, Zhang M.	Front Chem	10.3389/fchem.2021.815189	2021
Phylogeny	Legionella confirmation using real-time PCR and SYTO9 is an alternative to current methodology.	Giglio S, Monis PT, Saint CP.	Appl Environ Microbiol	10.1128/aem.71.12.8944-8948.2005	2005
Enzymology	Development and evaluation of Chlamylege, a new commercial test allowing simultaneous detection and identification of Legionella, Chlamydophila pneumoniae, and Mycoplasma pneumoniae in clinical respiratory specimens by multiplex PCR.	Ginevra C, Barranger C, Ros A, Mory O, Stephan JL, Freymuth F, Joannes M, Pozzetto B, Grattard F.	J Clin Microbiol	10.1128/jcm.43.7.3247-3254.2005	2005
Biotechnology	Detection of Salmonella spp. in oysters by PCR.	Bej AK, Mahbubani MH, Boyce MJ, Atlas RM.	Appl Environ Microbiol	10.1128/aem.60.1.368-373.1994	1994
Enzymology	Rapid method for enumeration of viable Legionella pneumophila and other Legionella spp. in water.	Delgado-Viscogliosi P, Simonart T, Parent V, Marchand G, Dobbelaere M, Pierlot E, Pierzo V, Menard-Szczebara F, Gaudard-Ferveur E, Delabre K, Delattre JM.	Appl Environ Microbiol	10.1128/aem.71.7.4086-4096.2005	2005
Phylogeny	Legionella dresdenensis sp. nov., isolated from river water.	Luck PC, Jacobs E, Roske I, Schroter-Bobsin U, Dumke R, Gronow S	Int J Syst Evol Microbiol	10.1099/ijs.0.017863-0	2009

References

#4522	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 11884
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#34426	; Curators of the CIP;
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#122290	Collection of Institut Pasteur ; Curators of the CIP; CIP 103848
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Legionella rubrilucens (CCUG 29671, DSM 11884, ATCC 35304)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Metabolite utilization

Metabolite production

Enzymes

Pathway annotation

API zym

Isolation, sampling and environmental information

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_001468125

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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