Strain identifier

BacDive ID: 6731

Type strain: Yes

Species: Legionella rubrilucens

Strain Designation: WA-270A-C2

Strain history: CIP <- 1993, R.F. Benson, CDC: strain WA-270A-C2 <- G.W. Gormann

NCBI tax ID(s): 458 (species)

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General

@ref: 4522

BacDive-ID: 6731

DSM-Number: 11884

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-negative, rod-shaped

description: Legionella rubrilucens WA-270A-C2 is a microaerophile, mesophilic, Gram-negative bacterium of the family Legionellaceae.

NCBI tax id

  • NCBI tax id: 458
  • Matching level: species

strain history

@refhistory
4522<- H. E. Müller <- ATCC <- G. W. Gorman
67770GIFU 10743 <-- CDC WA-270A-C2.
122290CIP <- 1993, R.F. Benson, CDC: strain WA-270A-C2 <- G.W. Gormann

doi: 10.13145/bacdive6731.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Legionellales
  • family: Legionellaceae
  • genus: Legionella
  • species: Legionella rubrilucens
  • full scientific name: Legionella rubrilucens Brenner et al. 1985

@ref: 4522

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Legionellales

family: Legionellaceae

genus: Legionella

species: Legionella rubrilucens

full scientific name: Legionella rubrilucens Brenner et al. 1985

strain designation: WA-270A-C2

type strain: yes

Morphology

cell morphology

  • @ref: 122290
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 4522
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
4522BCYE-AGAR (DSMZ Medium 585)yeshttps://mediadive.dsmz.de/medium/585Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base
34426MEDIUM 23 - for Afipia and LegionellayesDistilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml)
122290CIP Medium 23yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=23

culture temp

@refgrowthtypetemperaturerange
4522positivegrowth37mesophilic
34426positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 4522
  • oxygen tolerance: microaerophile

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12229017632nitrate-reduction
12229016301nitrite-reduction

metabolite production

  • @ref: 122290
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
4522catalase+1.11.1.6
4522cytochrome-c oxidase-1.9.3.1
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin-3.4.21.1
68382trypsin-3.4.21.4
68382cystine arylamidase-3.4.11.3
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)-
68382esterase lipase (C 8)+
68382alkaline phosphatase+3.1.3.1
122290oxidase-
122290catalase+1.11.1.6
122290urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
4522-+-+-++/----++--------
122290-+++-++---++--------

Isolation, sampling and environmental information

isolation

@refcountryorigin.countrycontinentsample typegeographic location
4522USAUSANorth America
67770USAUSANorth AmericaTap waterLos Angeles, CA
122290United States of AmericaUSANorth AmericaOther, Tap waterLos Angeles, California

taxonmaps

  • @ref: 69479
  • File name: preview.99_1469.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_35;96_839;97_981;98_1159;99_1469&stattab=map
  • Last taxonomy: Legionella
  • 16S sequence: Z32643
  • Sequence Identity:
  • Total samples: 682
  • soil counts: 154
  • aquatic counts: 438
  • animal counts: 55
  • plant counts: 35

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
45221Risk group (German classification)
1222902Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Legionella rubrilucens strain MDC1576 16S ribosomal RNA gene, partial sequenceJF720409525ena458
20218L.rubrilucens (WA-270A-C2) gene for 16S ribosomal RNAZ326431456ena458
4522L.rubrilucens (ATCC 35304) gene for 16S rRNAX733981468ena458

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Legionella rubrilucens strain WA-270A-C2458.5wgspatric458
66792Legionella rubrilucens ATCC353042660238232draftimg458

GC content

  • @ref: 67770
  • GC-content: 52
  • method: thermal denaturation, midpoint method (Tm)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
gram-positiveno98.461no
anaerobicno97.964no
halophileno84.581no
spore-formingno93.692no
glucose-utilyes64.484no
aerobicno92.473yes
flagellatedno81.05no
thermophileno95.252yes
motileyes84.054no
glucose-fermentno89.184no

External links

@ref: 4522

culture collection no.: CCUG 29671, DSM 11884, ATCC 35304, NCTC 11987, JCM 7565, CIP 103848

straininfo link

  • @ref: 76201
  • straininfo: 40984

literature

  • topic: Phylogeny
  • Pubmed-ID: 20008105
  • title: Legionella dresdenensis sp. nov., isolated from river water.
  • authors: Luck PC, Jacobs E, Roske I, Schroter-Bobsin U, Dumke R, Gronow S
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.017863-0
  • year: 2009
  • mesh: Bacterial Proteins/genetics, Cysteine/metabolism, DNA, Bacterial/genetics, Fresh Water/analysis/*microbiology, Legionella/*classification/genetics/*isolation & purification/metabolism, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/genetics
  • topic2: Metabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
4522Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 11884)https://www.dsmz.de/collection/catalogue/details/culture/DSM-11884
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
34426Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15691
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
76201Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID40984.1StrainInfo: A central database for resolving microbial strain identifiers
122290Curators of the CIPCollection of Institut Pasteur (CIP 103848)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103848