Legionella anisa (DSM 17627, ATCC 35292, CIP 103870)

Name: Legionella anisa (DSM 17627, ATCC 35292, CIP 103870)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 6732

Type strain

Strain Designation WA-316-C3

Culture col. no. DSM 17627 ATCC 35292 CIP 103870 NCTC 11974 WDCM 00106 5 more

NCBI tax ID(s) 28082

Special Collections DSMZ CCUG JCM KCTC CIP

History DSMZ <- H. E. Müller, Medizinal-Untersuchungsamt, Braunschweig; <- ATCC; ATCC 35292 <- G. W. Gorman, CDC, Atlanta; WA-316-C3 JCM <-- GIFU 12075 <-- ATCC 35292 <-- G. W. Gorman WA-316-C3. CIP <- 1993, C. Tram, Inst. Pasteur, Paris, France <- ATCC <- G.W. Gorman: strain WA-316-C3

Links

Legionella anisa WA-316-C3 is a microaerophile, Gram-negative, rod-shaped bacterium that was isolated from tap water.

Gram-negative rod-shaped microaerophile genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 40977

NBIMCC 8852,ATCC 35292,NCTC 11974,CCUG 29665,CIP 103870,JCM 7573,KCTC 12010,BCRC 17043,CCRC 17043,DSM 17627,CECT 8177

@ref 20215

Last LPSN update 2026-05-29 08:26:28

Domain Bacteria

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Legionellales

Family Legionellaceae

Genus Legionella

Species Legionella anisa

Full scientific name Legionella anisa Gorman et al. 1985

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
116396	negative	rod-shaped
125439	negative		96.581
125438	negative		98.979

Colony morphology

6917

Incubation period3-7 days

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
6917	BCYE-AGAR (DSMZ Medium 585)	Medium recipe at MediaDive	Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base
41839	MEDIUM 23 - for Afipia and Legionella		Distilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml)
116396	CIP Medium 23	Medium recipe at CIP
116396	CIP Medium 748	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
6917	positive	growth	37
41839	positive	growth	37
49701	positive	growth	37
67770	positive	growth	37

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
6917	microaerophile
49701	microaerophile
125439	obligate aerobe	99.183

Spore formation

@ref	Spore formation	Confidence
125439		99.063

Observation

67770

Observationquinones: Q-10

Enzymes

@ref	Value	Activity	Ec
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
6917	catalase	+	1.11.1.6
116396	catalase	+	1.11.1.6
6917	cytochrome-c oxidase	+	1.9.3.1
68382	esterase (C 4)	+		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
116396	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
6917	-	+	+	+/-	-	+	-	+/-	-	-	+	+	-	-	-	-	-	-	-	-
116396	-	+	+	-	-	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-
6917	-	+	+	-	-	+	-	-	-	-	+/-	+	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Freshwater
#Engineered	#Built environment	#Water reservoir (Aquarium/pool)
#Engineered	#Built environment	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Latitude	Longitude	Sampling date
6917	tap water	Los Angeles	USA	USA	North America	34.0522	-118.244 34.0522/-118.244
49701	Tap water							1981
67770	Tap water	Los Angeles, CA	USA	USA	North America
116396	Environment, Tap water	Los Angeles, California	United States of America	USA	North America

Taxonmaps

69479

Global distribution of 16S sequence Z32635 (>99% sequence identity) for Legionella anisa from Microbeatlas

Aquatic Samples	179
Soil Samples	154
Animal Samples	45
Plant Samples	35
Total Samples	413

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
6917	2	Risk group (German classification) According to TRBA 466
116396	2	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
124043	ASM1993088v1 assembly for Legionella anisa FDAARGOS_1480	complete	GCA_019930885	28082.25		28082	94.33
66792	Legionella anisa strain FDAARGOS_1480	complete		28082.27		28082	91.97
66792	Legionella anisa strain FDAARGOS_1480	complete		28082.32		28082	91.97
66792	Legionella anisa strain FDAARGOS_1480	complete		28082.26		28082	91.97
66792	ASM146752v1 assembly for Legionella anisa WA-316-C3	scaffold	GCA_001467525	28082.12	2663763523	28082	59.81
66792	Leg_anisa_WA-316-C3.v1 assembly for Legionella anisa WA-316-C3	scaffold	GCA_900639785	28082.19	8005402247	28082	40.65

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	L.anisa (ATCC 35292) gene for 16S rRNA	X73394	1467	28082
20218	L.anisa (WA-316-C3) gene for small subunit ribosomal RNA	Z32635	1398	28082
6917	Legionella anisa 16S ribosomal RNA gene, partial sequence	AY744776	1486	28082
124043	Legionella anisa 16S-23S ribosomal RNA intergenic spacer, partial sequence and tRNA-Ala gene, complete sequence.	KM609988	548	28082
124043	Legionella anisa 16S-23S ribosomal RNA intergenic spacer, partial sequence and tRNA-Ile gene, complete sequence.	KM609989	694	28082

GC content

@ref	GC-content (mol%)	Method
67770	42	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM1993088v1 assembly for Legionella anisa FDAARGOS_1480
BV-BRC: 28082.25

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	99.18	no
125439	gram_stain	BacteriaNetⓘ	negative	96.58	no
125439	motility	BacteriaNetⓘ	yes	45.95	no
125439	spore_formation	BacteriaNetⓘ	no	99.06	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Legionella anisa strain FDAARGOS_1480 strain Not applicable
PATRIC: 28082.25

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	98.98	no
125438	anaerobic	anaerobicⓘ	no	98.13	yes
125438	spore-forming	spore-formingⓘ	no	89.90	no
125438	aerobic	aerobicⓘ	no	72.50	no
125438	thermophilic	thermophileⓘ	no	95.49	no
125438	flagellated	motile2+ⓘ	yes	64.11	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Impact of Chlorine Dioxide on Pathogenic Waterborne Microorganisms Occurring in Dental Chair Units.	Kruger TIM, Herzog S, Mellmann A, Kuczius T.	Microorganisms	10.3390/microorganisms11051123	2023
	Polyethersulfone (PES) Filters Improve the Recovery of Legionella spp. and Enhance Selectivity against Interfering Microorganisms in Water Samples.	Casino P, Lopez A, Peiro S, Rios S, Porta A, Agusti G, Terlevich D, Asensio D, Marques AM, Pique N.	Polymers (Basel)	10.3390/polym15122670	2023
Genetics	Combining Traditional and Molecular Techniques Supports the Discovery of a Novel Legionella Species During Environmental Surveillance in a Healthcare Facility.	Girolamini L, Pascale MR, Mazzotta M, Spiteri S, Marino F, Salaris S, Grottola A, Orsini M, Cristino S.	Front Microbiol	10.3389/fmicb.2022.900936	2022
	GVPC Medium Manufactured without Oxygen Improves the Growth of Legionella spp. and Exhibits Enhanced Selectivity Properties.	Casino P, Lopez A, Peiro S, Rios M, Rios S, Porta A, Agusti G, Asensio D, Marques AM, Pique N.	Microbiol Spectr	10.1128/spectrum.02401-21	2022
Phylogeny	Development of a Novel Peptide Nucleic Acid Probe for the Detection of Legionella spp. in Water Samples.	Nacher-Vazquez M, Barbosa A, Armelim I, Azevedo AS, Almeida GN, Pizarro C, Azevedo NF, Almeida C, Cerqueira L.	Microorganisms	10.3390/microorganisms10071409	2022
Phylogeny	Chronic endocarditis due to Legionella anisa: a first case difficult to diagnose.	Compain F, Bruneval P, Jarraud S, Perrot S, Aubert S, Napoly V, Ramahefasolo A, Mainardi JL, Podglajen I.	New Microbes New Infect	10.1016/j.nmni.2015.10.003	2015
Phylogeny	New Insight regarding Legionella Non-Pneumophila Species Identification: Comparison between the Traditional mip Gene Classification Scheme and a Newly Proposed Scheme Targeting the rpoB Gene.	Pascale MR, Salaris S, Mazzotta M, Girolamini L, Fregni Serpini G, Manni L, Grottola A, Cristino S.	Microbiol Spectr	10.1128/spectrum.01161-21	2021
Phylogeny	Legionella confirmation using real-time PCR and SYTO9 is an alternative to current methodology.	Giglio S, Monis PT, Saint CP.	Appl Environ Microbiol	10.1128/aem.71.12.8944-8948.2005	2005
Enzymology	Development and evaluation of Chlamylege, a new commercial test allowing simultaneous detection and identification of Legionella, Chlamydophila pneumoniae, and Mycoplasma pneumoniae in clinical respiratory specimens by multiplex PCR.	Ginevra C, Barranger C, Ros A, Mory O, Stephan JL, Freymuth F, Joannes M, Pozzetto B, Grattard F.	J Clin Microbiol	10.1128/jcm.43.7.3247-3254.2005	2005
	Pleural infection caused by Legionella anisa.	Bornstein N, Mercatello A, Marmet D, Surgot M, Deveaux Y, Fleurette J.	J Clin Microbiol	10.1128/jcm.27.9.2100-2101.1989	1989
Metabolism	Targeting species-specific low-affinity 16S rRNA binding sites by using peptide nucleic acids for detection of Legionellae in biofilms.	Wilks SA, Keevil CW.	Appl Environ Microbiol	10.1128/aem.02918-05	2006
Phylogeny	Characterization of a Legionella anisa strain isolated from a patient with pneumonia.	Thacker WL, Benson RF, Hawes L, Mayberry WR, Brenner DJ	J Clin Microbiol	10.1128/jcm.28.1.122-123.1990	1990
Phylogeny	Legionella anisa: a new species of Legionella isolated from potable waters and a cooling tower.	Gorman GW, Feeley JC, Steigerwalt A, Edelstein PH, Moss CW, Brenner DJ	Appl Environ Microbiol	10.1128/aem.49.2.305-309.1985	1985
Phylogeny	Characterization of a Novel Species of Legionella Isolated from a Healthcare Facility: Legionella resiliens sp. nov.	Cristino S, Pascale MR, Marino F, Derelitto C, Salaris S, Orsini M, Squarzoni S, Grottola A, Girolamini L.	Pathogens	10.3390/pathogens13030250	2024

References

#6917	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 17627
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#41839	; Curators of the CIP;
#49701	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 29665
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#116396	Collection of Institut Pasteur ; Curators of the CIP; CIP 103870
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Content

About us

Legionella anisa (DSM 17627, ATCC 35292, CIP 103870)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Observation

Enzymes

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_019930885

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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