Legionella dumoffii (DSM 17625, ATCC 33279, CCUG 11881)

Name: Legionella dumoffii (DSM 17625, ATCC 33279, CCUG 11881)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 6710

Type strain

Strain Designation NY23, NY 23

Culture col. no. DSM 17625 ATCC 33279 CCUG 11881 CIP 103876 NCTC 11370 2 more

NCBI tax ID(s) 463

Special Collections DSMZ CCUG CIP

History DSMZ <- H. E. Müller, Medizinal-Untersuchungsamt, Braunschweig; <- ATCC; ATCC 33279 <- R. E. Weaver; NY23 CIP <- 1993, C. Tram, Inst. Pasteur, Paris, France <- ATCC <- R.E. Weaver, CDC: strain NY 23

Links

Legionella dumoffii NY23 is a microaerophile bacterium that was isolated from cooling tower.

microaerophile genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 40425

ATCC 33279,CCUG 11881,NCTC 11370,CIP 103876,BCRC 17048,CCRC 17048,DSM 17625,CECT 7349

@ref 20215

Last LPSN update 2026-05-29 08:26:28

Domain Bacteria

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Legionellales

Family Legionellaceae

Genus Legionella

Species Legionella dumoffii

Full scientific name Legionella dumoffii Brenner et al. 1980

Synonyms (1)

Fluoribacter dumoffii

BacDive ID	Other strains from **Legionella dumoffii** (1)	Type strain
6711	L. dumoffii R28, CCUG 47789, DSM 25314, ATCC 35850, Wadsworth ...

Morphology

Cell morphology

@ref	Gram stain	Confidence
125439	negative	98.761
125438	negative	99.494

Colony morphology

6915

Incubation period2-3 days

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
6915	BCYE-AGAR (DSMZ Medium 585)	Medium recipe at MediaDive	Name: BCYE AGAR (DSMZ Medium 585) Composition: OXOID Legionella CYE-Agar base
38153	MEDIUM 23 - for Afipia and Legionella		Distilled water make up to (1000.000 ml);Legionella agar (37.000 g);Legionella - enrichment mixture (10.000 ml)
118976	CIP Medium 23	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
6915	positive	growth	37
38153	positive	growth	37

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
6915	microaerophile
125439	obligate aerobe	99.321

Spore formation

@ref	Spore formation	Confidence
125439		99.001

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	-	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
6915	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
6915	cytochrome-c oxidase	-	1.9.3.1
68382	esterase lipase (C 8)	-		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
68382	trypsin	-	3.4.21.4	from API zym
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
6915	-	+	+/-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Industrial	#Cooling tower

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
6915	cooling tower	New York	USA	USA	North America
45616	Water,cooling tower	NY,New York	USA	USA	North America
118976	Cooling tower	New York	United States of America	USA	North America

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
6915	2	Risk group (German classification) According to TRBA 466
118976	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM23616v1 assembly for Fluoribacter dumoffii NY 23	chromosome	GCA_000236165	1094715.3	2547132117	1094715	82.23
66792	48903_A01 assembly for Fluoribacter dumoffii NCTC11370	contig	GCA_900450695	463.7	2839406339	463	77.48
124043	ASM146760v1 assembly for Fluoribacter dumoffii NY 23	scaffold	GCA_001467605	1094715.5	2657245499	1094715	73.31

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
6915	L.dumoffii (ATCC 33279) gene for 16S rRNA	X73405	1467	1094715
124043	Fluoribacter dumoffii 16S-23S ribosomal RNA intergenic spacer, partial sequence and tRNA-Ala gene, complete sequence.	KM609992	509	463
124043	Fluoribacter dumoffii 16S-23S ribosomal RNA intergenic spacer, partial sequence and tRNA-Ile gene, complete sequence.	KM609993	525	463

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM23616v1 assembly for Fluoribacter dumoffii NY 23
BV-BRC: 1094715.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	99.32	no
125439	gram_stain	BacteriaNetⓘ	negative	98.76	no
125439	motility	BacteriaNetⓘ	no	64.74	no
125439	spore_formation	BacteriaNetⓘ	no	99.00	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Fluoribacter dumoffii NY 23
PATRIC: 1094715.5

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	99.49	no
125438	anaerobic	anaerobicⓘ	no	94.88	yes
125438	spore-forming	spore-formingⓘ	no	88.89	no
125438	aerobic	aerobicⓘ	no	80.40	yes
125438	thermophilic	thermophileⓘ	no	95.99	yes
125438	flagellated	motile2+ⓘ	yes	61.03	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Genotypic and phenotypic profiling of 127 Legionella pneumophila strains: Insights into regional spread.	Colautti A, Civilini M, Bortolomeazzi R, Franchi M, Felice A, De Martin S, Iacumin L.	PLoS One	10.1371/journal.pone.0307646	2024
Phylogeny	Approach to determine the diversity of Legionella species by nested PCR-DGGE in aquatic environments.	Huang WC, Tsai HC, Tao CW, Chen JS, Shih YJ, Kao PM, Huang TY, Hsu BM.	PLoS One	10.1371/journal.pone.0170992	2017
Phylogeny	Legionella spp. All Ears? The Broad Occurrence of Quorum Sensing Elements outside Legionella pneumophila.	Herran B, Greve P, Berjeaud JM, Bertaux J, Crepin A.	Genome Biol Evol	10.1093/gbe/evab032	2021
Pathogenicity	Choline Supplementation Sensitizes Legionella dumoffii to Galleria mellonella Apolipophorin III.	Palusinska-Szysz M, Zdybicka-Barabas A, Luchowski R, Reszczynska E, Smialek J, Mak P, Gruszecki WI, Cytrynska M.	Int J Mol Sci	10.3390/ijms21165818	2020
Enzymology	Draft genome sequences of two Legionella dumoffii strains, TEX-KL and NY-23.	Qin T, Cui Y, Cen Z, Liang T, Ren H, Yang X, Zhao X, Liu Z, Xu L, Li D, Song Y, Yang R, Shao Z, Song Y.	J Bacteriol	10.1128/jb.06352-11	2012
	Anti-Legionella dumoffii activity of Galleria mellonella defensin and apolipophorin III.	Palusinska-Szysz M, Zdybicka-Barabas A, Pawlikowska-Pawlega B, Mak P, Cytrynska M.	Int J Mol Sci	10.3390/ijms131217048	2012
	A new oligonucleotide microarray for detection of pathogenic and non-pathogenic Legionella spp.	Cao B, Liu X, Yu X, Chen M, Feng L, Wang L.	PLoS One	10.1371/journal.pone.0113863	2014
Metabolism	Legionella dumoffii utilizes exogenous choline for phosphatidylcholine synthesis.	Palusinska-Szysz M, Szuster-Ciesielska A, Kania M, Janczarek M, Chmiel E, Danikiewicz W.	Int J Mol Sci	10.3390/ijms15058256	2014
Phylogeny	Legionella confirmation using real-time PCR and SYTO9 is an alternative to current methodology.	Giglio S, Monis PT, Saint CP.	Appl Environ Microbiol	10.1128/aem.71.12.8944-8948.2005	2005
Enzymology	Development and evaluation of Chlamylege, a new commercial test allowing simultaneous detection and identification of Legionella, Chlamydophila pneumoniae, and Mycoplasma pneumoniae in clinical respiratory specimens by multiplex PCR.	Ginevra C, Barranger C, Ros A, Mory O, Stephan JL, Freymuth F, Joannes M, Pozzetto B, Grattard F.	J Clin Microbiol	10.1128/jcm.43.7.3247-3254.2005	2005
Enzymology	Multilocus enzyme analysis of Legionella dumoffii.	Woods TC, McKinney RM, Plikaytis BD, Steigerwalt AG, Bibb WF, Brenner DJ.	J Clin Microbiol	10.1128/jcm.26.5.799-803.1988	1988
Metabolism	MTOR-Driven Metabolic Reprogramming Regulates Legionella pneumophila Intracellular Niche Homeostasis.	Abshire CF, Dragoi AM, Roy CR, Ivanov SS.	PLoS Pathog	10.1371/journal.ppat.1006088	2016
	Characterization of the alternative sigma factor sigma54 and the transcriptional regulator FleQ of Legionella pneumophila, which are both involved in the regulation cascade of flagellar gene expression.	Jacobi S, Schade R, Heuner K.	J Bacteriol	10.1128/jb.186.9.2540-2547.2004	2004
Enzymology	Development of a new seminested PCR method for detection of Legionella species and its application to surveillance of legionellae in hospital cooling tower water.	Miyamoto H, Yamamoto H, Arima K, Fujii J, Maruta K, Izu K, Shiomori T, Yoshida S.	Appl Environ Microbiol	10.1128/aem.63.7.2489-2494.1997	1997
	Cloning and genetic characterization of the flagellum subunit gene (flaA) of Legionella pneumophila serogroup 1.	Heuner K, Bender-Beck L, Brand BC, Luck PC, Mann KH, Marre R, Ott M, Hacker J.	Infect Immun	10.1128/iai.63.7.2499-2507.1995	1995
Phylogeny	Legionella bozemanii serogroup 2: a new etiological agent.	Tang PW, Toma S, Moss CW, Steigerwalt AG, Cooligan TG, Brenner DJ.	J Clin Microbiol	10.1128/jcm.19.1.30-33.1984	1984
	DNA probe specific for Legionella pneumophila.	Grimont PA, Grimont F, Desplaces N, Tchen P.	J Clin Microbiol	10.1128/jcm.21.3.431-437.1985	1985
Pathogenicity	In vitro and in vivo antibacterial activities of AT-4140, a new broad-spectrum quinolone.	Nakamura S, Minami A, Nakata K, Kurobe N, Kouno K, Sakaguchi Y, Kashimoto S, Yoshida H, Kojima T, Ohue T.	Antimicrob Agents Chemother	10.1128/aac.33.8.1167	1989
Enzymology	Legionella sainthelensi: a new species of Legionella isolated from water near Mt. St. Helens.	Campbell J, Bibb WF, Lambert MA, Eng S, Steigerwalt AG, Allard J, Moss CW, Brenner DJ.	Appl Environ Microbiol	10.1128/aem.47.2.369-373.1984	1984
Phylogeny	Serological and genotypic diversity among serogroup 5- reacting environmental Legionella isolates.	Garrity GM, Elder EM, Davis B, Vickers RM, Brown A.	J Clin Microbiol	10.1128/jcm.15.4.646-653.1982	1982
	Genus-specific epitope on the 60-kilodalton Legionella heat shock protein recognized by a monoclonal antibody.	Steinmetz I, Rheinheimer C, Hubner I, Bitter-Suermann D.	J Clin Microbiol	10.1128/jcm.29.2.346-354.1991	1991
	Cross-reactions between Legionella pneumophila (serogroup 1) and twenty-eight other bacterial species, including other members of the family Legionellaceae.	Collins MT, Espersen F, Hoiby N, Cho SN, Friis-Moller A, Reif JS.	Infect Immun	10.1128/iai.39.3.1441-1456.1983	1983

References

#6915	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 17625
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#38153	; Curators of the CIP;
#45616	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 11881
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68382	Automatically annotated from API zym .
#118976	Collection of Institut Pasteur ; Curators of the CIP; CIP 103876
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Legionella dumoffii (DSM 17625, ATCC 33279, CCUG 11881)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Enzymes

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000236165

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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