Name: Schleiferilactobacillus harbinensis DSM 16991
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 6637

Type strain:

Species: Schleiferilactobacillus harbinensis

Culture col. no.: DSM 16991, CIP 109152, JCM 16178, CCUG 54937, AHU 1762, SBT 10908, LMG 24040, NBRC 100982

Strain history: CIP <- 2006, DSMZ <- H. Nakajima, SBT 10908 <- Y. Miyamoto

NCBI tax ID(s): 1122147 (strain), 304207 (species)

SI-ID 289862

DSM 16991, NBRC 100982, CIP 109152, CCUG 0100, LMG 24040, JCM 16178

Section

Schleiferilactobacillus harbinensis DSM 16991 is a microaerophile, mesophilic, Gram-positive bacterium that was isolated from chinese traditional fermented vegetable Suan cai.

Gram-positive
rod-shaped
mesophilic
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Lactobacillaceae*
	Genus	*Schleiferilactobacillus*
	Species	**Schleiferilactobacillus harbinensis**
	Full Scientific Name (LPSN)	Schleiferilactobacillus harbinensis (Miyamoto et al. 2006) Zheng et al. 2020

Synonym

Lactobacillus harbinensis

Information on morphological and physiological properties Morphology

[Ref.: #119839]	Gram stain	positive

[Ref.: #119839]	Cell shape	rod-shaped

[Ref.: #119839]	Motility	no

[Ref.: #60398]

Incubation period

2 days

Information on culture and growth conditions Culture and growth conditions

[Ref.: #6594]

Culture medium

MRS MEDIUM (DSMZ Medium 11)

[Ref.: #6594]

Culture medium growth

[Ref.: #6594]

Culture medium link

Medium recipe at MediaDive

[Ref.: #6594]

Culture medium composition

expand / minimize

[Ref.: #33998]

Culture medium

MEDIUM 40- for Lactobacillus and Leuconostoc

[Ref.: #33998]

Culture medium growth

[Ref.: #33998]

Culture medium composition

expand / minimize

[Ref.: #119839]

Culture medium

CIP Medium 40

[Ref.: #119839]

Culture medium growth

[Ref.: #119839]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #6594]	growth	37 °C
[Ref.: #33998]	growth	37 °C
[Ref.: #60398]	growth	37 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #6594]	Temperature range	mesophilic
[Ref.: #33998]	Temperature range	mesophilic
[Ref.: #60398]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #6594]	Oxygen tolerance	microaerophile
[Ref.: #60398]	Oxygen tolerance	anaerobe
[Ref.: #60398]	Oxygen tolerance	microaerophile
[Ref.: #119839]	Oxygen tolerance	facultative anaerobe

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	cellobiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	esculin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	maltose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #119839]	nitrate ChEBI	-	reduction
[Ref.: #119839]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #119839]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	+	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	+	3.2.1.31	* from API zym
[Ref.: #119839]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	+	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #119839]	oxidase	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #119839]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #6594]	14352	-	-	-	-	+	-	-	-	-	-	+	+	+	+	-	-	-	-	-	-	-	+	+	+	+	+	+	+	+	-	+	+	+	-	+	+	-	-	-	+	-	-	-	-	-	-	-	-	-	-
[Ref.: #6594]	14351	-	-	-	-	+	-	-	-	-	-	+	+	+	+	-	-	-	-	-	-	-	+	+	+	+	+	+	+	+	-	+	+	+	+	+	+	+	-	-	+	+	-	+	-	-	-	-	+	-	-
[Ref.: #119839]	11484	not determinedn.d.	-	-	-	+	-	-	-	-	-	+	+	+	+	-	-	-	-	-	-	-	+/-	+	+	+	+	+	+	+	-	+/-	+	+	+	+	+	+	-	-	+	-	-	-	-	-	-	-	+	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #119839]	11685	-	+	-	-	-	+	-	-	-	-	+	+	+	-	+	+	+	+	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #6594]	Sample type/isolated from	chinese traditional fermented vegetable Suan cai
[Ref.: #6594]	Geographic location (country and/or sea, region)	Harbin
[Ref.: #6594]	Country	China
[Ref.: #6594]	Country ISO 3 Code	CHN
[Ref.: #6594]	Continent	Asia

[Ref.: #60398]	Sample type/isolated from	Fermented vegetables 'Suan cai'
[Ref.: #60398]	Geographic location (country and/or sea, region)	Harbin
[Ref.: #60398]	Country	China
[Ref.: #60398]	Country ISO 3 Code	CHN
[Ref.: #60398]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Traditional fermented vegetables 'Suan cai'
[Ref.: #67770]	Geographic location (country and/or sea, region)	Harbin
[Ref.: #67770]	Country	China
[Ref.: #67770]	Country ISO 3 Code	CHN
[Ref.: #67770]	Continent	Asia

[Ref.: #119839]	Sample type/isolated from	Food, Traditional fermented vegetables Suan cai
[Ref.: #119839]	Country	China
[Ref.: #119839]	Country ISO 3 Code	CHN
[Ref.: #119839]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Food production	#Vegetable (incl. Grains)
#Engineered	#Food production	#Fermented

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LC519855 (>99% sequence identity) for Schleiferilactobacillus harbinensis from Microbeatlas

Aquatic Samples	395
Soil Samples	104
Animal Samples	981
Plant Samples	58
Total Samples	1538

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #6594]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #119839]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #6594]	Lactobacillus harbinensis gene for 16S rRNA, partial sequence, strain:SBT10908	AB196123	1507	ENA	304207 tax ID
[Ref.: #67770]	Lactobacillus harbinensis JCM 16178 gene for 16S rRNA, partial sequence	LC519855	1513	ENA	304207 tax ID

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Schleiferilactobacillus harbinensis DSM 16991	GCA_000425885	scaffold	GenBank	1122147 tax ID
[Ref.: #67770]	Schleiferilactobacillus harbinensis DSM 16991	GCA_001436325	scaffold	GenBank	1122147 tax ID
[Ref.: #66792]	Lactobacillus harbinensis DSM 16991	1122147.3	wgs	PATRIC	1122147 tax ID	*
[Ref.: #66792]	Lactobacillus harbinensis DSM 16991	1122147.4	wgs	PATRIC	1122147 tax ID	*
[Ref.: #66792]	Lactobacillus harbinensis DSM 16991	2690316249	draft	JGI IMG/M	1122147 tax ID	*
[Ref.: #66792]	Lactobacillus harbinensis DSM 16991	2523533608	draft	JGI IMG/M	1122147 tax ID	*

[Ref.: #6594]	GC-content	53 mol%
[Ref.: #67770]	GC-content	53.3 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67770]	GC-content	53.8 mol%	genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Lactobacillus harbinensis DSM 16991 PATRIC: 1122147.3
[Ref.: #69480]	motile	no	93.099	no
[Ref.: #69480]	flagellated	no	96.907	no
[Ref.: #69480]	gram-positive	yes	97.374	no
[Ref.: #69480]	anaerobic	no	91.538	no
[Ref.: #69480]	halophile	yes	80.972	no
[Ref.: #69480]	spore-forming	no	90.053	no
[Ref.: #69480]	thermophile	no	97.114	yes
[Ref.: #69480]	glucose-util	yes	91.2	no
[Ref.: #69480]	aerobic	no	93.953	yes
[Ref.: #69480]	glucose-ferment	yes	89.928	no

Availability in culture collections External links

[Ref.: #6594]

Culture collection no.

DSM 16991, CIP 109152, JCM 16178, CCUG 54937, AHU 1762, SBT 10908, LMG 24040, NBRC 100982

[Ref.: #76111]

SI-ID 289862

Literature:

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Lactobacillus harbinensis sp. nov., consisted of strains isolated from traditional fermented vegetables 'Suan cai' in Harbin, Northeastern China and Lactobacillus perolens DSM 12745.	Miyamoto M, Seto Y, Hao DH, Teshima T, Sun YB, Kabuki T, Yao LB, Nakajima H	Syst Appl Microbiol	10.1016/j.syapm.2005.04.001	2005	*

References

#6594

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 16991

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#33998 ; Curators of the CIP;
#60398 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 54937

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#76111

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID289862.1 )

#119839 Collection of Institut Pasteur ; Curators of the CIP; CIP 109152
* These data were automatically processed and therefore are not curated

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Lactobacillus harbinensis DSM 16991

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