Schleiferilactobacillus harbinensis (DSM 16991, CIP 109152, JCM 16178)

Name: Schleiferilactobacillus harbinensis (DSM 16991, CIP 109152, JCM 16178)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 6637

Type strain

Culture col. no. DSM 16991 CIP 109152 JCM 16178 CCUG 54937 AHU 1762 3 more

NCBI tax ID(s) 1122147 304207

Special Collections DSMZ CCUG JCM CIP

History DSMZ <- H. Nakajima; SBT 10908 <- Y. Miyamoto CIP <- 2006, DSMZ JCM <-- DSM 16991 <-- H. Nakajima SBT 10908 <-- Y. Miyamoto. CIP <- 2006, DSMZ <- H. Nakajima, SBT 10908 <- Y. Miyamoto

Links

Schleiferilactobacillus harbinensis DSM 16991 is a microaerophile, Gram-positive, rod-shaped bacterium that was isolated from chinese traditional fermented vegetable Suan cai.

Gram-positive rod-shaped microaerophile genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 289862

DSM 16991,NBRC 100982,CIP 109152,CCUG 0100,LMG 24040,JCM 16178,CCUG 54937T

@ref 20215

Last LPSN update 2026-05-29 08:26:27

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Lactobacillales

Family Lactobacillaceae

Genus Schleiferilactobacillus

Species Schleiferilactobacillus harbinensis

Full scientific name Schleiferilactobacillus harbinensis (Miyamoto et al. 2006) Zheng et al. 2020

Synonyms (1)

Lactobacillus harbinensis

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
119839	positive	rod-shaped

Colony morphology

60398

Incubation period2 days

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
6594	MRS MEDIUM (DSMZ Medium 11)	Medium recipe at MediaDive	Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
33998	MEDIUM 40- for Lactobacillus and Leuconostoc		Distilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
119839	CIP Medium 40	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
6594	positive	growth	37
33998	positive	growth	37
60398	positive	growth	37
67770	positive	growth	37

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
6594	microaerophile
60398	anaerobe
60398	microaerophile
119839	facultative anaerobe
125439	facultative anaerobe	91.838

Spore formation

@ref	Spore formation	Confidence
125439		99.155

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	+	builds acid from	from API 50CH acid
68371	18305 ChEBI	arbutin	+	builds acid from	from API 50CH acid
68371	17057 ChEBI	cellobiose	+	builds acid from	from API 50CH acid
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	+	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	+	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	-	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68371	4853 ChEBI	esculin	+	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	+	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	+	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
68371	17306 ChEBI	maltose	+	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	+	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	+	builds acid from	from API 50CH acid
119839	17632 ChEBI	nitrate	-	reduction
119839	16301 ChEBI	nitrite	-	reduction
68371	0 ChEBI	Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371	0 ChEBI	Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	+	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	+	builds acid from	from API 50CH acid
68371	17992 ChEBI	sucrose	+	builds acid from	from API 50CH acid
68371	27082 ChEBI	trehalose	+	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Metabolite production

@ref	Chebi-ID	Metabolite	Production
119839	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	+	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	+	3.2.1.31	from API zym
119839	catalase	-	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	-		from API zym
68382	esterase lipase (C 8)	-		from API zym
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
119839	oxidase	-
68382	trypsin	-	3.4.21.4	from API zym
119839	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
119839	-	+	-	-	-	+	-	-	-	-	+	+	+	-	+	+	+	+	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
6594	-	-	-	-	+	-	-	-	-	-	+	+	+	+	-	-	-	-	-	-	-	+	+	+	+	+	+	+	+	-	+	+	+	-	+	+	-	-	-	+	-	-	-	-	-	-	-	-	-	-
6594	-	-	-	-	+	-	-	-	-	-	+	+	+	+	-	-	-	-	-	-	-	+	+	+	+	+	+	+	+	-	+	+	+	+	+	+	+	-	-	+	+	-	+	-	-	-	-	+	-	-
119839	not determinedn.d.	-	-	-	+	-	-	-	-	-	+	+	+	+	-	-	-	-	-	-	-	+/-	+	+	+	+	+	+	+	-	+/-	+	+	+	+	+	+	-	-	+	-	-	-	-	-	-	-	+	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Food production	#Vegetable (incl. Grains)
#Engineered	#Food production	#Fermented

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
6594	chinese traditional fermented vegetable Suan cai	Harbin	China	CHN	Asia
60398	Fermented vegetables 'Suan cai'	Harbin	China	CHN	Asia
67770	Traditional fermented vegetables 'Suan cai'	Harbin	China	CHN	Asia
119839	Food, Traditional fermented vegetables Suan cai		China	CHN	Asia

Taxonmaps

69479

Global distribution of 16S sequence LC519855 (>99% sequence identity) for Schleiferilactobacillus harbinensis from Microbeatlas

Aquatic Samples	395
Soil Samples	104
Animal Samples	981
Plant Samples	58
Total Samples	1538

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
6594	1	Risk group (German classification) According to TRBA 466
119839	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM42588v1 assembly for Schleiferilactobacillus harbinensis DSM 16991	scaffold	GCA_000425885	1122147.3	2523533608	1122147	55.75
67770	ASM143632v1 assembly for Schleiferilactobacillus harbinensis DSM 16991	scaffold	GCA_001436325	1122147.4	2690316249	1122147	40.49

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
6594	Lactobacillus harbinensis gene for 16S rRNA, partial sequence, strain:SBT10908	AB196123	1507	304207
67770	Lactobacillus harbinensis JCM 16178 gene for 16S rRNA, partial sequence	LC519855	1513	304207
124043	Lactobacillus harbinensis gene for 16S rRNA, partial sequence, strain: NBRC 100982.	AB681318	1496	304207

GC content

@ref	GC-content (mol%)	Method
6594	53
67770	53.3	high performance liquid chromatography (HPLC)
67770	53.8	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM42588v1 assembly for Schleiferilactobacillus harbinensis DSM 16991
BV-BRC: 1122147.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	91.84	no
125439	gram_stain	BacteriaNetⓘ	negative	71.82	no
125439	motility	BacteriaNetⓘ	no	62.13	no
125439	spore_formation	BacteriaNetⓘ	no	99.16	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Lactobacillus harbinensis DSM 16991
PATRIC: 1122147.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	86.37	no
125438	anaerobic	anaerobicⓘ	no	81.05	no
125438	spore-forming	spore-formingⓘ	no	77.50	no
125438	aerobic	aerobicⓘ	no	92.83	yes
125438	thermophilic	thermophileⓘ	no	96.44	yes
125438	flagellated	motile2+ⓘ	no	89.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Novel cross-feeding human gut microbes metabolizing tryptophan to indole-3-propionate.	Zund JN, Mujezinovic D, Reichlin M, Pluss S, Caflisch M, Robinson S, Lacroix C, Pugin B.	Gut Microbes	10.1080/19490976.2025.2501195	2025
Genetics	Effects of simulated space environmental conditions on cleanroom microbes.	Cassilly CD, Chander AM, Vaughn JA, Kunstman KJ, Green SJ, Venkateswaran K, Bertone PF, Bahr CW, Marcella SA, Morris HC.	Front Microbiol	10.3389/fmicb.2025.1600106	2025
Genetics	Exploration of Alicyclobacillus spp. Genome in Search of Antibiotic Resistance.	Bucka-Kolendo J, Kiousi DE, Dekowska A, Mikolajczuk-Szczyrba A, Karadedos DM, Michael P, Galanis A, Sokolowska B.	Int J Mol Sci	10.3390/ijms25158144	2024
Metabolism	Roles of human colonic bacteria in pectin utilization and associated cross-feeding networks revealed using synthetic co-cultures.	Solvang M, Farquharson FM, Horgan G, Pisano S, Holck J, Zeuner B, Russell WR, Louis P.	Microbiology (Reading)	10.1099/mic.0.001559	2025
	Draft Genome Sequence of the Ascomycete Xylaria multiplex DSM 110363.	Buttner E, Liers C, Richter A, Hofrichter M, Kellner H.	Microbiol Resour Announc	10.1128/mra.00262-20	2020
Genetics	Genomic analysis of Enterococcus faecium strain RAOG174 associated with acute chorioamnionitis carried antibiotic resistance gene: is it time for precise microbiological identification for appropriate antibiotic use?	Pongchaikul P, Romero R, Mongkolsuk P, Vivithanaporn P, Wongsurawat T, Jenjaroenpun P, Nitayanon P, Thaipisuttikul I, Kamlungkuea T, Singsaneh A, Santanirand P, Chaemsaithong P.	BMC Genomics	10.1186/s12864-023-09511-1	2023
Metabolism	Characterization of canthaxanthin isomers isolated from a new soil Dietzia sp. and their antioxidant activities.	Venugopalan V, Tripathi SK, Nahar P, Saradhi PP, Das RH, Gautam HK.	J Microbiol Biotechnol	10.4014/jmb.1203.03032	2013
Proteome	DnaK duplication and specialization in bacteria correlates with increased proteome complexity.	Pan Z, Zhuo L, Wan T-y, Chen R-y, Li Y-z.	mSystems	10.1128/msystems.01154-23	2024
	MYXO-CTERM sorting tag directs proteins to the cell surface via the type II secretion system.	Sah GP, Cao P, Wall D.	Mol Microbiol	10.1111/mmi.14473	2020
Metabolism	Bioinformatic and Functional Characterization of Hsp70s in Myxococcus xanthus.	Pan Z, Zhang Z, Zhuo L, Wan TY, Li YZ.	mSphere	10.1128/msphere.00305-21	2021
Pathogenicity	Structure of ribosome-bound azole-modified peptide phazolicin rationalizes its species-specific mode of bacterial translation inhibition.	Travin DY, Watson ZL, Metelev M, Ward FR, Osterman IA, Khven IM, Khabibullina NF, Serebryakova M, Mergaert P, Polikanov YS, Cate JHD, Severinov K.	Nat Commun	10.1038/s41467-019-12589-5	2019
	Complete Genome Sequence and Comparative Genomics of a Novel Myxobacterium Myxococcus hansupus.	Sharma G, Narwani T, Subramanian S.	PLoS One	10.1371/journal.pone.0148593	2016
	Lactic acid bacteria and yeast strains isolated from fermented fish (Budu) identified as candidate ruminant probiotics based on in vitro rumen fermentation characteristics.	Ardani LR, Marlida Y, Zain M, Jamsari J, Fassah DM.	Vet World	10.14202/vetworld.2023.395-402	2023
Enzymology	Isolation and Characterization of Lactobacillus spp. from Kefir Samples in Malaysia.	Talib N, Mohamad NE, Yeap SK, Hussin Y, Aziz MNM, Masarudin MJ, Sharifuddin SA, Hui YW, Ho CL, Alitheen NB.	Molecules	10.3390/molecules24142606	2019
	Kefir and Intestinal Microbiota Modulation: Implications in Human Health.	Peluzio MDCG, Dias MME, Martinez JA, Milagro FI.	Front Nutr	10.3389/fnut.2021.638740	2021
	Levilactobacillus yiduensis sp. nov., Isolated from Zha-chili in Yidu County, Hubei Province, China.	Dong Y, Wang Y, Zhao H, Zhang Y, Zhang H, Sun Y, Guo Z.	Curr Microbiol	10.1007/s00284-023-03211-5	2023
Phylogeny	Tumebacillus algifaecis sp. nov., isolated from decomposing algal scum.	Wu YF, Zhang B, Xing P, Wu QL, Liu SJ.	Int J Syst Evol Microbiol	10.1099/ijs.0.000240	2015
Phylogeny	Bifidobacterium aesculapii sp. nov., from the faeces of the baby common marmoset (Callithrix jacchus).	Modesto M, Michelini S, Stefanini I, Ferrara A, Tacconi S, Biavati B, Mattarelli P.	Int J Syst Evol Microbiol	10.1099/ijs.0.056937-0	2014
Phylogeny	Tumebacillus flagellatus sp. nov., an alpha-amylase/pullulanase-producing bacterium isolated from cassava wastewater.	Wang Q, Xie N, Qin Y, Shen N, Zhu J, Mi H, Huang R.	Int J Syst Evol Microbiol	10.1099/ijs.0.045351-0	2013
Phylogeny	Microbacterium immunditiarum sp. nov., an actinobacterium isolated from landfill surface soil, and emended description of the genus Microbacterium.	Krishnamurthi S, Bhattacharya A, Schumann P, Dastager SG, Tang SK, Li WJ, Chakrabarti T.	Int J Syst Evol Microbiol	10.1099/ijs.0.033373-0	2012
Phylogeny	Lysobacter ruishenii sp. nov., a chlorothalonil-degrading bacterium isolated from a long-term chlorothalonil-contaminated soil.	Wang GL, Wang L, Chen HH, Shen B, Li SP, Jiang JD.	Int J Syst Evol Microbiol	10.1099/ijs.0.020990-0	2011
Phylogeny	Tsukamurella soli sp. nov., isolated from soil.	Weon HY, Yoo SH, Anandham R, Schumann P, Kroppenstedt RM, Kwon SW, Stackebrandt E.	Int J Syst Evol Microbiol	10.1099/ijs.0.014852-0	2010
Phylogeny	Tsukamurella carboxydivorans sp. nov., a carbon monoxide-oxidizing actinomycete.	Park SW, Kim SM, Park ST, Kim YM.	Int J Syst Evol Microbiol	10.1099/ijs.0.005959-0	2009
Phylogeny	Lysobacter oryzae sp. nov., isolated from the rhizosphere of rice (Oryza sativa L.).	Aslam Z, Yasir M, Jeon CO, Chung YR.	Int J Syst Evol Microbiol	10.1099/ijs.0.000588-0	2009
Metabolism	Lactobacillus harbinensis sp. nov., consisted of strains isolated from traditional fermented vegetables 'Suan cai' in Harbin, Northeastern China and Lactobacillus perolens DSM 12745.	Miyamoto M, Seto Y, Hao DH, Teshima T, Sun YB, Kabuki T, Yao LB, Nakajima H	Syst Appl Microbiol	10.1016/j.syapm.2005.04.001	2005

References

#6594	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 16991
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#33998	; Curators of the CIP;
#60398	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 54937
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#119839	Collection of Institut Pasteur ; Curators of the CIP; CIP 109152
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Rate our new design

Content

About us

Schleiferilactobacillus harbinensis (DSM 16991, CIP 109152, JCM 16178)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Metabolite utilization

Metabolite production

Enzymes

API zym

API 50CHac

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000425885

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

Help

Social Media