Name: Limosilactobacillus pontis DSM 8475
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 6562

Type strain:

Species: Limosilactobacillus pontis

Culture col. no.: DSM 8475, ATCC 51518, JCM 11051, LMG 14187, CCUG 33456, LTH 2587, CIP 104232

Strain history: CIP <- 1994, P. Stolz, Inst. für Lebensmitteltechnol., Germany: strain LTH 2587 <- Hohenheim Univ., Germany

NCBI tax ID(s): 1423794 (strain), 35787 (species)

SI-ID 8943

ATCC 51518, CCUG 33456, CIP 104232, JCM 11051, NCIMB 13406, DSM 8475, CCM 4540, LMG14187, KCTC 5074, VTT E-052865

Other strains from Limosilactobacillus pontis

L. pontis DSM 8476, ATCC 51519, LMG 14188, ...
L. pontis Cla-CZ-76, DSM 109116

Section

Limosilactobacillus pontis DSM 8475 is an anaerobe, mesophilic, Gram-positive bacterium that was isolated from rye sourdough.

Gram-positive
rod-shaped
anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Lactobacillales*
	Family	*Lactobacillaceae*
	Genus	*Limosilactobacillus*
	Species	**Limosilactobacillus pontis**
	Full Scientific Name (LPSN)	Limosilactobacillus pontis (Vogel et al. 1994) Zheng et al. 2020

Synonym

Lactobacillus pontis

Information on morphological and physiological properties Morphology

[Ref.: #121939]	Gram stain	positive

[Ref.: #121939]	Cell shape	rod-shaped

[Ref.: #121939]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #3288]

Culture medium

LACTOBACILLUS MEDIUM III (DSMZ Medium 638)

[Ref.: #3288]

Culture medium growth

[Ref.: #3288]

Culture medium link

Medium recipe at MediaDive

[Ref.: #3288]

Culture medium composition

expand / minimize

[Ref.: #38168]

Culture medium

MEDIUM 42 - for Lactobacillus pontis

[Ref.: #38168]

Culture medium growth

[Ref.: #38168]

Culture medium composition

expand / minimize

[Ref.: #121939]

Culture medium

CIP Medium 42

[Ref.: #121939]

Culture medium growth

[Ref.: #121939]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #3288]	growth	30 °C
[Ref.: #38168]	growth	30 °C
[Ref.: #51530]	growth	37 °C
[Ref.: #67770]	growth	30 °C
[Ref.: #121939]	growth	15-45 °C

[Ref.: #3288]	Temperature range	mesophilic
[Ref.: #38168]	Temperature range	mesophilic
[Ref.: #51530]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #51530]	Oxygen tolerance	anaerobe
[Ref.: #121939]	Oxygen tolerance	facultative anaerobe

[Ref.: #3288]	Murein short key	A21.04
[Ref.: #3288]	Murein types	A4ß L-Orn-D-Asp

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	cellobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	esculin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gentiobiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	maltose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #121939]	nitrate ChEBI	-	reduction
[Ref.: #121939]	nitrate ChEBI	+	respiration
[Ref.: #121939]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #121939]		acetoin ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #121939]	alcohol dehydrogenase	+	1.1.1.1
[Ref.: #121939]	catalase	-	1.11.1.6
[Ref.: #121939]	lysine decarboxylase	-	4.1.1.18
[Ref.: #121939]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #121939]	oxidase	-

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #3288]	14260	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
[Ref.: #121939]	9544	not determinedn.d.	-	-	-	-	+	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	+	-	-	-	+/-	-	-	-	-	-	-	-	-	-	-	-	+/-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #3288]	Sample type/isolated from	rye sourdough
[Ref.: #3288]	Geographic location (country and/or sea, region)	Germany, Stuttgart
[Ref.: #3288]	Country	Germany
[Ref.: #3288]	Country ISO 3 Code	DEU
[Ref.: #3288]	Continent	Europe

[Ref.: #51530]	Sample type/isolated from	Sour dough
[Ref.: #51530]	Geographic location (country and/or sea, region)	Stuttgart
[Ref.: #51530]	Country	Germany
[Ref.: #51530]	Country ISO 3 Code	DEU
[Ref.: #51530]	Continent	Europe

[Ref.: #67770]	Sample type/isolated from	Sourdough

[Ref.: #121939]	Sample type/isolated from	Rye sourdough
[Ref.: #121939]	Country	Germany
[Ref.: #121939]	Country ISO 3 Code	DEU
[Ref.: #121939]	Continent	Europe
[Ref.: #121939]	Isolation date	1988
* marker position based on {}

Isolation sources categories

#Engineered	#Food production	#Food
#Host	#Plants	#Herbaceous plants (Grass,Crops)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LC145562 (>99% sequence identity) for Limosilactobacillus from Microbeatlas

Aquatic Samples	1526
Soil Samples	1269
Animal Samples	35132
Plant Samples	754
Total Samples	38681

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #3288]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #121939]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #3288]	L.pontis (LTH 2587) 16S rRNA gene	X76329	1570	ENA	35787 tax ID
[Ref.: #67770]	Lactobacillus pontis gene for 16S ribosomal RNA, partial sequence, strain: JCM 11051	LC145562	1458	ENA	35787 tax ID
[Ref.: #20218]	Lactobacillus pontis 16S rRNA gene, type strain LMG 14187	AJ422032	1480	ENA	35787 tax ID	*
[Ref.: #20218]	Lactobacillus pontis gene for 16S rRNA, partial sequence, strain: JCM 11051	AB289267	731	ENA	35787 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Limosilactobacillus pontis DSM 8475	GCA_001435345	scaffold	GenBank	1423794 tax ID
[Ref.: #66792]	Lactobacillus pontis DSM 8475	1423794.3	wgs	PATRIC	1423794 tax ID	*
[Ref.: #66792]	Limosilactobacillus pontis DSM 8475	2700989158	draft	JGI IMG/M	1423794 tax ID	*

[Ref.: #3288]	GC-content	53 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67770]	GC-content	53.3 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Lactobacillus pontis DSM 8475 PATRIC: 1423794.3
[Ref.: #69480]	motile	no	96.181	no
[Ref.: #69480]	gram-positive	yes	96.693	no
[Ref.: #69480]	anaerobic	no	50	yes
[Ref.: #69480]	halophile	yes	94.029	no
[Ref.: #69480]	spore-forming	no	97.198	no
[Ref.: #69480]	glucose-util	yes	88.84	no
[Ref.: #69480]	thermophile	no	99.771	yes
[Ref.: #69480]	aerobic	no	97.39	yes
[Ref.: #69480]	flagellated	no	98.582	no
[Ref.: #69480]	glucose-ferment	yes	89.004	no

Availability in culture collections External links

[Ref.: #3288]

Culture collection no.

DSM 8475, ATCC 51518, JCM 11051, LMG 14187, CCUG 33456, LTH 2587, CIP 104232

[Ref.: #76034]

SI-ID 8943

Literature:

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Glutathione reductase from Lactobacillus sanfranciscensis DSM20451T: contribution to oxygen tolerance and thiol exchange reactions in wheat sourdoughs.	Jansch A, Korakli M, Vogel RF, Ganzle MG	Appl Environ Microbiol	10.1128/AEM.02322-06	2007	*
Phylogeny	Identification of lactobacilli from sourdough and description of Lactobacillus pontis sp. nov.	Vogel RF, Bocker G, Stolz P, Ehrmann M, Fanta D, Ludwig W, Pot B, Kersters K, Schleifer KH, Hammes WP	Int J Syst Bacteriol	10.1099/00207713-44-2-223	1994	*

References

#3288

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 8475

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#38168 ; Curators of the CIP;
#51530 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 33456

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68371

Automatically annotated from API 50CH acid .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#76034

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID8943.1 )

#121939 Collection of Institut Pasteur ; Curators of the CIP; CIP 104232
* These data were automatically processed and therefore are not curated

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Lactobacillus pontis DSM 8475

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