Liquorilactobacillus mali (DSM 20444, ATCC 27053, NCIB 10560)

Name: Liquorilactobacillus mali (DSM 20444, ATCC 27053, NCIB 10560)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 6482

Type strain

Strain Designation J12

Culture col. no. DSM 20444 ATCC 27053 NCIB 10560 J12 VKM B-1600 16 more

NCBI tax ID(s) 1618

Special Collections DSMZ CCUG JCM KCTC CIP

History <- ATCC <- J.G. Carr, J12 ATCC 27053 <-- J. G. Carr J12. CIP <- 1988, DSM <- ATCC, Lactobacillus yamanashiensis subsp. mali <- J.G. Carr: strain J 12, Lactobacillus mali

Links

Liquorilactobacillus mali J12 is a mesophilic prokaryote that was isolated from apple juice from cider press.

mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 92312

LMG 6899,ATCC 27053,CCM 2878,CCRC 12940,CIP 103142,DSM 20444,JCM 1116,LMG 11716,NCFB 2168,NCIMB 10560,NCIB 10560,CCUG 30141,NCDO 2168,CCUG 32228,CCRC 14057,VKM B-1600,KCTC 3596,NRRL B-4563,BCRC 12940,BCRC 14057,CCT 2570,NBIMCC 3316,NBRC 102159

@ref 20215

Last LPSN update 2026-02-09 11:19:13

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Lactobacillales

Family Lactobacillaceae

Genus Liquorilactobacillus

Species Liquorilactobacillus mali

Full scientific name Liquorilactobacillus mali (Carr and Davies 1970) Zheng et al. 2020

Synonyms (3)

Lactobacillus yamanashiensis subsp. mali
Lactobacillus mali
Lactobacillus yamanashiensis

BacDive ID	Other strains from **Liquorilactobacillus mali** (7)	Type strain
6483	L. mali 239, DSM 20483, ATCC 27304, JCM 1153, BCRC ...
163410	L. mali JCM 2773
163412	L. mali JCM 2775
164826	L. mali JCM 3821, ATCC 27054, NCIMB 10561
164827	L. mali JCM 3822, ATCC 27055, NCIMB 10562
165855	L. mali JCM 8611
165856	L. mali JCM 8612

Morphology

Cell morphology

@ref	Gram stain	Confidence
125438	positive	91.178

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
8816	MRS MEDIUM (DSMZ Medium 11)	Medium recipe at MediaDive	Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
34914	MEDIUM 41- for Lactobacillus, Leuconostoc, Weissella, Pediococcus, Sporolactobacillus inulinus		Distilled water make up to (1000.000 ml);Man Rogosa Sharp broth (55.000 g)
120756	CIP Medium 41	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
8816	positive	growth	30	mesophilic
34914	positive	growth	30	mesophilic
67770	positive	growth	30	mesophilic

Physiology and metabolism

Murein

@ref	Murein short key	Type
8816	A31	A1gamma m-Dpm-direct

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	+	builds acid from	from API 50CH acid
68371	18305 ChEBI	arbutin	+	builds acid from	from API 50CH acid
68371	17057 ChEBI	cellobiose	+	builds acid from	from API 50CH acid
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	-	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	+	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	+	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	+	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68371	4853 ChEBI	esculin	+	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	+	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	+	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
68371	17306 ChEBI	maltose	-	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	+	builds acid from	from API 50CH acid
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371		Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	+	builds acid from	from API 50CH acid
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
68371	17992 ChEBI	sucrose	+	builds acid from	from API 50CH acid
68371	27082 ChEBI	trehalose	+	builds acid from	from API 50CH acid
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
8816	-	-	-	-	-	-	-	-	-	-	-	+	+	+	+	-	-	-	+	-	-	-	+	+	+	+	+	+	-	-	-	+	+	-	-	-	-	-	-	+	-	-	+	-	-	-	-	-	-	-
8816	-	-	-	-	-	-	-	-	-	-	-	+	+	+	+	+	-	-	+	+	-	-	+	+	+	+	+	+	-	-	-	+	+	-	-	-	-	-	-	+	-	-	+	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Food production	#Beverage

Isolation

@ref	Sample type
8816	apple juice from cider press
67770	Apple juice from cider press
120756	Food, Apple juice from cider press

Taxonmaps

69479

Global distribution of 16S sequence LC064888 (>99% sequence identity) for Liquorilactobacillus from Microbeatlas

Aquatic Samples	161
Soil Samples	144
Animal Samples	1322
Plant Samples	293
Total Samples	1920

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
8816	1	Risk group (German classification) According to TRBA 466
120756	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM26041v1 assembly for Liquorilactobacillus mali KCTC 3596 = DSM 20444	contig	GCA_000260415	1046596.4	2513237368	1046596	51.4
67770	ASM143653v1 assembly for Liquorilactobacillus mali KCTC 3596 = DSM 20444	scaffold	GCA_001436535	1046596.6	2671180581	1046596	34.22
67770	LmaliDSM20444T assembly for Liquorilactobacillus mali KCTC 3596 = DSM 20444	contig	GCA_000276905		2534682326	1046596	25.93

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Lactobacillus mali KCTC 3596 = DSM 20444 16S ribosomal RNA gene, partial sequence	M58824	1502	1046596
20218	Lactobacillus mali gene for 16S rRNA, partial sequence, strain: JCM 1116	AB289195	633	1046596
20218	Lactobacillus mali gene for 16S rRNA, partial sequence, strain: NBRC 102159	AB326352	1485	1046596
67770	Lactobacillus mali gene for 16S ribosomal RNA, partial sequence, strain: JCM 1116	LC064888	1441	1046596

GC content

@ref	GC-content (mol%)	Method
8816	36.9
8816	32.5
67770	36.1	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Liquorilactobacillus mali KCTC 3596 = DSM 20444
NCBI: GCA_001436535

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	87.30	no
125439	gram_stain	BacteriaNetⓘ	variable	74.90	no
125439	motility	BacteriaNetⓘ	yes	72.70	no
125439	spore_formation	BacteriaNetⓘ	yes	70.00	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Lactobacillus mali KCTC 3596 = DSM 20444
PATRIC: 1046596.6

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	91.18	no
125438	anaerobic	anaerobicⓘ	no	82.50	no
125438	spore-forming	spore-formingⓘ	no	72.66	no
125438	aerobic	aerobicⓘ	no	94.19	no
125438	thermophilic	thermophileⓘ	no	91.81	no
125438	flagellated	motile2+ⓘ	yes	56.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Flagellar Genes Are Associated with the Colonization Persistence Phenotype of the Drosophila melanogaster Microbiota.	Morgan SJ, Chaston JM.	Microbiol Spectr	10.1128/spectrum.04585-22	2023
	Heterologous expression and antimicrobial potential of class II bacteriocins.	Elisa Heesemann Rosenkilde C, Olsen Lutzhoft D, Vazquez-Uribe R, Otto Alexander Sommer M.	Gut Microbes	10.1080/19490976.2024.2369338	2024
Transcriptome	Genetic variability of mutans streptococci revealed by wide whole-genome sequencing.	Song L, Wang W, Conrads G, Rheinberg A, Sztajer H, Reck M, Wagner-Dobler I, Zeng AP.	BMC Genomics	10.1186/1471-2164-14-430	2013
	Candida tropicalis, Clavispora lusitaniae, Limosilactobacillus fermentum, Liquorilactobacillus mali, and Leuconostoc pseudomesenteroides are associated with ethanol in Malian traditional fermented milk products.	Sissoko S, Konate S, Armstrong N, Traore I, Kone AK, Djimde A, Thera MA, Million M, Tidjani Alou M.	Microb Pathog	10.1016/j.micpath.2025.107298	2025
	Genomic and functional evaluation of exopolysaccharide produced by Liquorilactobacillus mali t6-52: technological implications.	Wu M, Pakroo S, Nadai C, Molinelli Z, Speciale I, De Castro C, Tarrah A, Yang J, Giacomini A, Corich V.	Microb Cell Fact	10.1186/s12934-024-02431-z	2024
Genetics	Unveiling Bacterial Diversity in Portuguese Red Wine Effluents Through a Metagenomic Approach.	Gomes AG, Sousa AC, Carreira JS, Oliveira A, Justino MC, Santos CA.	Microorganisms	10.3390/microorganisms13092192	2025
	Molecular approaches to uncover phage-lactic acid bacteria interactions in a model community simulating fermented beverages.	Ledormand P, Desmasures N, Bernay B, Goux D, Rue O, Midoux C, Monnet C, Dalmasso M.	Food Microbiol	10.1016/j.fm.2022.104069	2022
Genetics	Multi-platform metagenomic characterization of the microbial community during spontaneous cacao fermentation.	Tigrero-Vaca J, Villavicencio-Vasquez M, Coronel J, Cevallos-Cevallos JM.	Front Bioeng Biotechnol	10.3389/fbioe.2025.1630515	2025
	Leuconostoc mesenteroides and Liquorilactobacillus mali strains, isolated from Algerian food products, are producers of the postbiotic compounds dextran, oligosaccharides and mannitol.	Zarour K, Zeid AF, Mohedano ML, Prieto A, Kihal M, Lopez P.	World J Microbiol Biotechnol	10.1007/s11274-024-03913-3	2024
	Backslopping Time, Rinsing of the Grains During Backslopping, and Incubation Temperature Influence the Water Kefir Fermentation Process.	Laureys D, Leroy F, Vandamme P, De Vuyst L.	Front Microbiol	10.3389/fmicb.2022.871550	2022
	Identification of Key Parameters Inducing Microbial Modulation during Backslopped Kombucha Fermentation.	Daval C, Tran T, Verdier F, Martin A, Alexandre H, Grandvalet C, Tourdot-Marechal R.	Foods	10.3390/foods13081181	2024
	Phages Shape Microbial Dynamics and Metabolism of a Model Community Mimicking Cider, a Fermented Beverage.	Ledormand P, Desmasures N, Schlusselhuber M, Sesboue A, Ledauphin J, Dalmasso M.	Viruses	10.3390/v14102283	2022
Pathogenicity	Phage Encounters Recorded in CRISPR Arrays in the Genus Oenococcus.	Barchi Y, Philippe C, Chaib A, Oviedo-Hernandez F, Claisse O, Le Marrec C.	Viruses	10.3390/v15010015	2022
	Biotechnological potential and genomic analysis of the Leuconostoc mesenteroides F17 and F18 strains isolated from camel milk.	Zeid AF, Zarour K, Mohedano ML, Diez-Ozaeta I, Lopez P.	BMC Microbiol	10.1186/s12866-025-04349-x	2025
	Kombucha tea as an anti-hyperglycemic agent in humans with diabetes - a randomized controlled pilot investigation.	Mendelson C, Sparkes S, Merenstein DJ, Christensen C, Sharma V, Desale S, Auchtung JM, Kok CR, Hallen-Adams HE, Hutkins R.	Front Nutr	10.3389/fnut.2023.1190248	2023
	Microbial Ecology of Artisanal Feta and Kefalograviera Cheeses, Part I: Bacterial Community and Its Functional Characteristics with Focus on Lactic Acid Bacteria as Determined by Culture-Dependent Methods and Phenotype Microarrays.	Tsigkrimani M, Bakogianni M, Paramithiotis S, Bosnea L, Pappa E, Drosinos EH, Skandamis PN, Mataragas M.	Microorganisms	10.3390/microorganisms10010161	2022
	Persistent Neutrophil Infiltration and Unique Ocular Surface Microbiome Typify Dupilumab-Associated Conjunctivitis in Patients with Atopic Dermatitis.	Patra V, Woltsche N, Cerpes U, Bokanovic D, Repelnig M, Joshi A, Perchthaler I, Fischl M, Vocanson M, Bordag N, Durdevic M, Woltsche J, Quehenberger F, Legat F, Wedrich A, Horwath-Winter J, Wolf P.	Ophthalmol Sci	10.1016/j.xops.2023.100340	2024
	Role of Lipoteichoic Acid from the Genus Apilactobacillus in Inducing a Strong IgA Response.	Matsuzaki C, Shiraishi T, Chiou TY, Nakashima Y, Higashimura Y, Yokota SI, Yamamoto K, Takahashi T.	Appl Environ Microbiol	10.1128/aem.00190-22	2022
Enzymology	Methods for Detection, Extraction, Purification, and Characterization of Exopolysaccharides of Lactic Acid Bacteria-A Systematic Review.	Yadav MK, Song JH, Vasquez R, Lee JS, Kim IH, Kang DK.	Foods	10.3390/foods13223687	2024
Genetics	Draft genome sequence of Lactobacillus mali KCTC 3596.	Kim DW, Choi SH, Kang A, Nam SH, Kim DS, Kim RN, Kim A, Park HS	J Bacteriol	10.1128/JB.05686-11	2011
Phylogeny	Sphingomonas panacis sp. nov., isolated from rhizosphere of rusty ginseng.	Singh P, Singh P, Kim YJ, Hoang VA, Farh Mel-A, Yang DC.	Antonie Van Leeuwenhoek	10.1007/s10482-015-0527-y	2015
Phylogeny	Lactobacillus sucicola sp. nov., a motile lactic acid bacterium isolated from oak tree (Quercus sp.) sap.	Irisawa T, Okada S	Int J Syst Evol Microbiol	10.1099/ijs.0.006478-0	2009
Phylogeny	Lactobacillus oeni sp. nov., from wine.	Manes-Lazaro R, Ferrer S, Rossello-Mora R, Pardo I	Int J Syst Evol Microbiol	10.1099/ijs.0.007567-0	2009
Phylogeny	Lactobacillus hordei sp. nov., a bacteriocinogenic strain isolated from malted barley.	Rouse S, Canchaya C, van Sinderen D	Int J Syst Evol Microbiol	10.1099/ijs.0.65584-0	2008

References

#8816	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20444
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#34914	; Curators of the CIP;
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68371	Automatically annotated from API 50CH acid .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#120756	Collection of Institut Pasteur ; Curators of the CIP; CIP 103142
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Liquorilactobacillus mali (DSM 20444, ATCC 27053, NCIB 10560)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Murein

Metabolite utilization

API 50CHac

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000260415

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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