Strain identifier

BacDive ID: 6482

Type strain: Yes

Species: Liquorilactobacillus mali

Strain Designation: J12

Strain history: CIP <- 1988, DSM <- ATCC, Lactobacillus yamanashiensis subsp. mali <- J.G. Carr: strain J 12, Lactobacillus mali

NCBI tax ID(s): 1618 (species)

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General

@ref: 8816

BacDive-ID: 6482

DSM-Number: 20444

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, Gram-positive

description: Liquorilactobacillus mali J12 is a mesophilic, Gram-positive bacterium that was isolated from apple juice from cider press.

NCBI tax id

  • NCBI tax id: 1618
  • Matching level: species

strain history

@refhistory
8816<- ATCC <- J.G. Carr, J12
67770ATCC 27053 <-- J. G. Carr J12.
120756CIP <- 1988, DSM <- ATCC, Lactobacillus yamanashiensis subsp. mali <- J.G. Carr: strain J 12, Lactobacillus mali

doi: 10.13145/bacdive6482.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Lactobacillales
  • family: Lactobacillaceae
  • genus: Liquorilactobacillus
  • species: Liquorilactobacillus mali
  • full scientific name: Liquorilactobacillus mali (Carr and Davies 1970) Zheng et al. 2020

  • synonyms

    @refsynonym
    20215Lactobacillus yamanashiensis subsp. mali
    20215Lactobacillus yamanashiensis
    20215Lactobacillus mali

@ref: 8816

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Lactobacillales

family: Lactobacillaceae

genus: Liquorilactobacillus

species: Liquorilactobacillus mali

full scientific name: Liquorilactobacillus mali (Carr and Davies 1970) Zheng et al. 2020

strain designation: J12

type strain: yes

Morphology

cell morphology

  • @ref: 69480
  • gram stain: positive
  • confidence: 100

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8816MRS MEDIUM (DSMZ Medium 11)yeshttps://mediadive.dsmz.de/medium/11Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
34914MEDIUM 41- for Lactobacillus, Leuconostoc, Weissella, Pediococcus, Sporolactobacillus inulinusyesDistilled water make up to (1000.000 ml);Man Rogosa Sharp broth (55.000 g)
120756CIP Medium 41yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=41

culture temp

@refgrowthtypetemperaturerange
8816positivegrowth30mesophilic
34914positivegrowth30mesophilic
67770positivegrowth30mesophilic

Physiology and metabolism

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.986

murein

  • @ref: 8816
  • murein short key: A31
  • type: A1gamma m-Dpm-direct

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-tagatose+builds acid from16443
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371gentiobiose+builds acid from28066
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose+builds acid from27082
68371sucrose+builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose+builds acid from17057
68371salicin+builds acid from17814
68371esculin+builds acid from4853
68371arbutin+builds acid from18305
68371amygdalin+builds acid from27613
68371N-acetylglucosamine+builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-mannitol+builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-sorbose+builds acid from17266
68371D-mannose+builds acid from16024
68371D-fructose+builds acid from15824
68371D-glucose+builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-xylose-builds acid from65327
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754

API 50CHac

@refQGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
8816-----------++++---+---++++++---++------+--+-------
8816-----------+++++--++--++++++---++------+--+-------

Isolation, sampling and environmental information

isolation

@refsample type
8816apple juice from cider press
67770Apple juice from cider press
120756Food, Apple juice from cider press

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Food production
  • Cat3: #Beverage

taxonmaps

  • @ref: 69479
  • File name: preview.99_4379.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_45;96_1207;97_1430;98_2298;99_4379&stattab=map
  • Last taxonomy: Liquorilactobacillus
  • 16S sequence: LC064888
  • Sequence Identity:
  • Total samples: 1920
  • soil counts: 144
  • aquatic counts: 161
  • animal counts: 1322
  • plant counts: 293

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
88161Risk group (German classification)
1207561Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Lactobacillus mali KCTC 3596 = DSM 20444 16S ribosomal RNA gene, partial sequenceM588241502ena1046596
20218Lactobacillus mali gene for 16S rRNA, partial sequence, strain: JCM 1116AB289195633ena1046596
20218Lactobacillus mali gene for 16S rRNA, partial sequence, strain: NBRC 102159AB3263521485ena1046596
67770Lactobacillus mali gene for 16S ribosomal RNA, partial sequence, strain: JCM 1116LC0648881441ena1046596

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Lactobacillus mali KCTC 3596 = DSM 204441046596.6wgspatric1046596
66792Lactobacillus mali KCTC 3596 = DSM 204441046596.4wgspatric1046596
66792Liquorilactobacillus mali KCTC 35962534682326draftimg1046596
66792Liquorilactobacillus mali KCTC 35962513237368draftimg1046596
66792Liquorilactobacillus mali DSM 204442671180581draftimg1046596
67770Liquorilactobacillus mali KCTC 3596 = DSM 20444GCA_000276905contigncbi1046596
67770Liquorilactobacillus mali KCTC 3596 = DSM 20444GCA_001436535scaffoldncbi1046596
67770Liquorilactobacillus mali KCTC 3596 = DSM 20444GCA_000260415contigncbi1046596

GC content

@refGC-contentmethod
881636.9
881632.5
6777036.1genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno52no
motileyes61.992no
flagellatedno82.276no
gram-positiveyes92.181no
anaerobicno91.225no
aerobicno94.846no
halophileyes51.651no
spore-formingno87.26no
thermophileno97.137yes
glucose-utilyes90.569no
glucose-fermentyes82.407no

External links

@ref: 8816

culture collection no.: DSM 20444, ATCC 27053, NCIB 10560, JCM 1116, BCRC 12940, BCRC 14057, CCM 2878, CCUG 30141, CCUG 32228, CIP 103142, KCTC 3596, LMG 6899, NBIMCC 3316, NBRC 102159, NCFB 2168, NCIMB 10560, NRIC 1692, NRRL B-4563, VKM B-1600, NCDO 2168

straininfo link

  • @ref: 75955
  • straininfo: 92312

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny18768596Lactobacillus hordei sp. nov., a bacteriocinogenic strain isolated from malted barley.Rouse S, Canchaya C, van Sinderen DInt J Syst Evol Microbiol10.1099/ijs.0.65584-02008Bacterial Typing Techniques, Bacteriocins/*biosynthesis, Catalase/metabolism, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Genes, rRNA, Hordeum/*microbiology, Lactobacillus/*classification/genetics/*isolation & purification/physiology, Locomotion, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic AcidGenetics
Phylogeny19567555Lactobacillus oeni sp. nov., from wine.Manes-Lazaro R, Ferrer S, Rossello-Mora R, Pardo IInt J Syst Evol Microbiol10.1099/ijs.0.007567-02009Anaerobiosis, Bacterial Typing Techniques, Catalase/metabolism, Cluster Analysis, DNA Fingerprinting/methods, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Lactobacillus/*classification/genetics/*isolation & purification/physiology, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, Polymorphism, Restriction Fragment Length, RNA, Ribosomal, 16S/genetics, Random Amplified Polymorphic DNA Technique, Sequence Analysis, DNA, Spain, Wine/*microbiologyGenetics
Phylogeny19625442Lactobacillus sucicola sp. nov., a motile lactic acid bacterium isolated from oak tree (Quercus sp.) sap.Irisawa T, Okada SInt J Syst Evol Microbiol10.1099/ijs.0.006478-02009DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Lactic Acid/*metabolism, Lactobacillus/classification/genetics/*isolation & purification/metabolism, Molecular Sequence Data, Phylogeny, Quercus/*microbiology, RNA, Ribosomal, 16S/geneticsMetabolism
Genetics21742889Draft genome sequence of Lactobacillus mali KCTC 3596.Kim DW, Choi SH, Kang A, Nam SH, Kim DS, Kim RN, Kim A, Park HSJ Bacteriol10.1128/JB.05686-112011Base Composition, Beverages/microbiology, DNA, Bacterial/*chemistry/*genetics, Food Industry, *Genome, Bacterial, Lactobacillus/*genetics/isolation & purification, Malus/microbiology, Molecular Sequence Data, *Sequence Analysis, DNATranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8816Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 20444)https://www.dsmz.de/collection/catalogue/details/culture/DSM-20444
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
34914Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/14907
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68371Automatically annotated from API 50CH acid
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
75955Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID92312.1StrainInfo: A central database for resolving microbial strain identifiers
120756Curators of the CIPCollection of Institut Pasteur (CIP 103142)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103142