Name: Halomonas halodenitrificans DSM 735
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 6014

Type strain:

Species: Halomonas halodenitrificans

Culture col. no.: DSM 735, ATCC 13511, CCM 286, NCMB 700, CIP 105456, IAM 13950, IFO 14912, NCIMB 700, CECT 5012

Strain history: CIP <- 1998, NCIMB <- M. Ingram <- N.E. Gibbons, Micrococcus halodenitrificans

NCBI tax ID(s): 1121941 (strain), 28252 (species)

SI-ID 35825

ATCC 13511, DSM 735, CCM 286, NCMB 700, CIP 105456, IAM 13950, IFO 14912, NCIMB 700, CECT 5012, NBRC 14912, KCTC 5069

Section

Halomonas halodenitrificans DSM 735 is a mesophilic, Gram-negative, oval-shaped bacterium that was isolated from wiltshire bacon curing brine.

Gram-negative
oval-shaped
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Gammaproteobacteria*
	Order	*Oceanospirillales*
	Family	*Halomonadaceae*
	Genus	*Halomonas*
	Species	**Halomonas halodenitrificans**
	Full Scientific Name (LPSN)	Halomonas halodenitrificans (Robinson and Gibbons 1952) Dobson and Franzmann 1996

	Synonym	Paracoccus halodenitrificans
	Synonym	"Micrococcus* halodenitrificans"*

Information on morphological and physiological properties Morphology

[Ref.: #119801]	Gram stain	negative

[Ref.: #119801]	Cell shape	oval-shaped

[Ref.: #119801]	Motility	no

Information on culture and growth conditions Culture and growth conditions

[Ref.: #379]

Culture medium

MEDIUM 514 PLUS ADDITIONAL SALT (DSMZ Medium 514b)

[Ref.: #379]

Culture medium growth

[Ref.: #379]

Culture medium link

Medium recipe at MediaDive

[Ref.: #379]

Culture medium composition

expand / minimize

[Ref.: #39298]

Culture medium

MEDIUM 192 - for Alteromonas rubra

[Ref.: #39298]

Culture medium growth

[Ref.: #39298]

Culture medium composition

expand / minimize

[Ref.: #119801]

Culture medium

CIP Medium 192

[Ref.: #119801]

Culture medium growth

[Ref.: #119801]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #379]		growth	28 °C
[Ref.: #39298]		growth	25 °C
[Ref.: #119801]		growth	15-30 °C
[Ref.: #119801]	no	growth	5 °C
[Ref.: #119801]	no	growth	37 °C
[Ref.: #119801]	no	growth	41 °C
[Ref.: #119801]	no	growth	45 °C

[Ref.: #379]	Temperature range	mesophilic
[Ref.: #39298]	Temperature range	mesophilic
[Ref.: #119801]	Temperature range	psychrophilic
[Ref.: #119801]	Temperature range	mesophilic
[Ref.: #119801]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #119801]		NaCl		growth	2-10	%
[Ref.: #119801]		NaCl	no	growth	0	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #119801]	esculin ChEBI	-	hydrolysis
[Ref.: #119801]	glucose ChEBI	-	degradation
[Ref.: #119801]	hippurate ChEBI	-	hydrolysis
[Ref.: #119801]	malonate ChEBI	-	assimilation
[Ref.: #119801]	nitrate ChEBI	-	builds gas from
[Ref.: #119801]	nitrate ChEBI	+	reduction
[Ref.: #119801]	nitrite ChEBI	-	builds gas from
[Ref.: #119801]	nitrite ChEBI	-	reduction

	Metabolite production	Metabolite	Production
[Ref.: #119801]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #119801]		acetoin ChEBI	-
[Ref.: #119801]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #119801]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #119801]	amylase	-
[Ref.: #119801]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #119801]	caseinase	-	3.4.21.50
[Ref.: #119801]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	-		* from API zym
[Ref.: #119801]	gamma-glutamyltransferase	+	2.3.2.2
[Ref.: #119801]	gelatinase	-
[Ref.: #119801]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #119801]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #119801]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #119801]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #119801]	oxidase	-
[Ref.: #119801]	phenylalanine ammonia-lyase	-	4.3.1.24
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #119801]	tryptophan deaminase	-
[Ref.: #119801]	tween esterase	-
[Ref.: #119801]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #119801]	1161	not determinedn.d.	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #119801]	8714	-	-	+	-	-	+	-	-	-	-	-	+	-	-	-	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #379]	Sample type/isolated from	wiltshire bacon curing brine
[Ref.: #379]	Country	Canada
[Ref.: #379]	Country ISO 3 Code	CAN
[Ref.: #379]	Continent	North America

[Ref.: #119801]	Sample type/isolated from	Bacon curing brine
[Ref.: #119801]	Geographic location (country and/or sea, region)	Wiltshire
[Ref.: #119801]	Country	United Kingdom
[Ref.: #119801]	Country ISO 3 Code	GBR
[Ref.: #119801]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Engineered	#Food production	#Meat
#Condition	#Saline	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence L04942 (>99% sequence identity) for Halomonas from Microbeatlas

Aquatic Samples	19
Soil Samples	8
Animal Samples	12
Plant Samples	7
Total Samples	46

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #379]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #119801]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #379]	Paracoccus halodenitrificans 16S ribosomal RNA sequence	L04942	1531	ENA	28252 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Halomonas halodenitrificans DSM 735	GCA_000620045	scaffold	GenBank	1121941 tax ID	*
[Ref.: #66792]	Halomonas halodenitrificans DSM 735	1121941.3	wgs	PATRIC	1121941 tax ID	*
[Ref.: #66792]	Halomonas halodenitrificans DSM 735	2556921012	draft	JGI IMG/M	1121941 tax ID	*

[Ref.: #379]

GC-content

65.0 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Halomonas halodenitrificans DSM 735 DSM 735 NCBI: GCA_000620045.1
[Ref.: #69480]	motile	no	74.204	no
[Ref.: #69480]	flagellated	no	93.812	no
[Ref.: #69480]	gram-positive	no	98.925	no
[Ref.: #69480]	anaerobic	no	98.885	no
[Ref.: #69480]	halophile	yes	94.529	no
[Ref.: #69480]	spore-forming	no	96.897	no
[Ref.: #69480]	thermophile	no	99.406	yes
[Ref.: #69480]	glucose-util	yes	87.283	no
[Ref.: #69480]	aerobic	yes	83.399	no
[Ref.: #69480]	glucose-ferment	no	77.61	no

Availability in culture collections External links

[Ref.: #379]

Culture collection no.

DSM 735, ATCC 13511, CCM 286, NCMB 700, CIP 105456, IAM 13950, IFO 14912, NCIMB 700, CECT 5012

[Ref.: #75500]

SI-ID 35825

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Halomonas icarae sp. nov., a moderately halophilic bacterium isolated from beach soil in India.	Pandiyan K, Kushwaha P, Bagul SY, Chakdar H, Madhaiyan M, Krishnamurthi S, Kumar P, Karthikeyan N, Singh A, Kumar M, Singh UB, Saxena AK	Int J Syst Evol Microbiol	10.1099/ijsem.0.004611	2020	*
Metabolism	Chip calorimetry for the monitoring of whole cell biotransformation.	Maskow T, Lerchner J, Peitzsch M, Harms H, Wolf G	J Biotechnol	10.1016/j.jbiotec.2005.10.008	2005	*
Metabolism	Thermokinetic description of anaerobic growth of Halomonas halodenitrificans using a static microcalorimetric ampoule technique.	Maskow T, Babel W	J Biotechnol	10.1016/s0168-1656(02)00341-3	2003	*

References

#379	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 735

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#39298 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#75500

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID35825.1 )

#119801 Collection of Institut Pasteur ; Curators of the CIP; CIP 105456
* These data were automatically processed and therefore are not curated

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Halomonas halodenitrificans DSM 735 DSM 735

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