Name: Bacillus amyloliquefaciens F
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 598

Type strain:

Species: Bacillus amyloliquefaciens

Strain Designation: F

Culture col. no.: DSM 7, ATCC 23350, CCUG 28519, NRRL B-14393, Fukumoto strain F

Strain history: <- ATCC <- L.L. Campbell, F (Bacillus subtilis)

NCBI tax ID(s): 692420 (strain), 1390 (species)

Other strains from Bacillus amyloliquefaciens

Section

Bacillus amyloliquefaciens F is a spore-forming, psychrophilic, Gram-positive bacterium that produces antibiotic compounds and was isolated from soil and industrial amylase fermentations.

antibiotic compound production
Gram-positive
motile
rod-shaped
spore-forming
psychrophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Bacillus*
	Species	**Bacillus amyloliquefaciens**
	Full Scientific Name (PNU)	Bacillus amyloliquefaciens (ex Fukomoto 1943) Priest et al. 1987

Information on morphological and physiological properties Morphology

[Ref.: #24474]	Gram stain	positive
[Ref.: #24474]	Cell length	1.8-3.0 µm
[Ref.: #24474]	Cell width	0.7-0.9 µm
[Ref.: #24474]	Cell shape	rod-shaped
[Ref.: #24474]	Motility	yes
[Ref.: #24474]	Flagellum arrangement	peritrichous

[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #69480]	Motility	yes Confidence in %91.406

[Ref.: #24474]

Cultivation medium used

medium of Gordon et al.

Information on culture and growth conditions Culture and growth conditions

[Ref.: #24474]

Culture medium

medium of Gordon et al.

[Ref.: #24474]

Culture medium growth

[Ref.: #35323]

Culture medium

MEDIUM 3 - Columbia agar

[Ref.: #35323]

Culture medium growth

[Ref.: #35323]

Culture medium composition

expand / minimize

[Ref.: #2015]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #2015]

Culture medium growth

[Ref.: #2015]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #2015]

Culture medium composition

expand / minimize

	Kind of temperature		Temperature
[Ref.: #2015]		growth	30 °C
[Ref.: #24474]	no	growth	<15 °C
[Ref.: #24474]	no	growth	>50 °C
[Ref.: #24474]		optimum	30-40 °C
[Ref.: #35323]		growth	30 °C

[Ref.: #2015]	Temperature range	mesophilic
[Ref.: #24474]	Temperature range	psychrophilic
[Ref.: #24474]	Temperature range	thermophilic
[Ref.: #35323]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #24474]	Spore description	central or paracentral
[Ref.: #24474]	Type of spore	spore
[Ref.: #24474]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

[Ref.: #2015]	Name of produced compound	alpha amylase
[Ref.: #2015]	Name of produced compound	1-deoxynojirimycin
[Ref.: #2015]	Name of produced compound	inhibitors for glycoside hydrolases
[Ref.: #2015]	Name of produced compound	restriction endonuclease BamFI (BamHI)
[Ref.: #24474]	Name of produced compound	Acetoin

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #24474]		NaCl		growth	5	%(w/v)
[Ref.: #24474]		NaCl		growth	10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #24474]	acetoin ChEBI	+	other
[Ref.: #24474]	adenine ChEBI	-	other
[Ref.: #24474]	adenine ChEBI	-	degradation
[Ref.: #24474]	allantoin ChEBI	-	hydrolysis
[Ref.: #24474]	alpha-D-glucose ChEBI	+	builds acid from
[Ref.: #24474]	alpha-lactose ChEBI	+	builds acid from
[Ref.: #24474]	arbutin ChEBI	+	hydrolysis
[Ref.: #24474]	carboxymethylcellulose ChEBI	-	degradation
[Ref.: #24474]	casein	+	other
[Ref.: #24474]	casein	+	degradation
[Ref.: #24474]	cellobiose ChEBI	+	builds acid from
[Ref.: #24474]	cellulose ChEBI	-	other
[Ref.: #24474]	citrate ChEBI	+	growth
[Ref.: #24474]	citrate ChEBI	+	carbon source
[Ref.: #24474]	dna ChEBI	+/-	degradation
[Ref.: #24474]	elastin ChEBI	+	other
[Ref.: #24474]	elastin ChEBI	+	degradation
[Ref.: #24474]	esculin ChEBI	+	hydrolysis
[Ref.: #24474]	fructose ChEBI	+	builds acid from
[Ref.: #24474]	gelatin ChEBI	+	other
[Ref.: #24474]	glucose ChEBI	+	builds acid from
[Ref.: #24474]	glycerol ChEBI	+	builds acid from
[Ref.: #24474]	guanine ChEBI	-	other
[Ref.: #24474]	guanine ChEBI	-	degradation
[Ref.: #24474]	hypoxanthine ChEBI	-	other
[Ref.: #24474]	hypoxanthine ChEBI	-	degradation
[Ref.: #24474]	lactose ChEBI	+	builds acid from
[Ref.: #24474]	maltose ChEBI	+	builds acid from
[Ref.: #24474]	mannitol ChEBI	+	builds acid from
[Ref.: #24474]	mannose ChEBI	+	builds acid from
[Ref.: #24474]	nitrate ChEBI	+	reduction
[Ref.: #24474]	pectin ChEBI	-	degradation
[Ref.: #24474]	raffinose ChEBI	+	builds acid from
[Ref.: #24474]	salicin ChEBI	+	builds acid from
[Ref.: #24474]	sorbitol ChEBI	+	builds acid from
[Ref.: #24474]	starch ChEBI	+	other
[Ref.: #24474]	starch ChEBI	+	degradation
[Ref.: #24474]	sucrose ChEBI	+	builds acid from
[Ref.: #24474]	testosterone ChEBI	-	other
[Ref.: #24474]	testosterone ChEBI	-	degradation
[Ref.: #24474]	trehalose ChEBI	+	builds acid from
[Ref.: #24474]	tributyrin ChEBI	+	other
[Ref.: #24474]	tributyrin ChEBI	+	degradation
[Ref.: #24474]	tween 20 ChEBI	+	other
[Ref.: #24474]	tween 20 ChEBI	+	degradation
[Ref.: #24474]	tween 40 ChEBI	+	other
[Ref.: #24474]	tween 40 ChEBI	+	degradation
[Ref.: #24474]	tween 60 ChEBI	+	other
[Ref.: #24474]	tween 60 ChEBI	+	degradation
[Ref.: #24474]	tyrosine ChEBI	-	other
[Ref.: #24474]	tyrosine ChEBI	-	degradation
[Ref.: #24474]	urea ChEBI	-	other
[Ref.: #24474]	urea ChEBI	-	hydrolysis
[Ref.: #24474]	xanthine ChEBI	-	other
[Ref.: #24474]	xanthine ChEBI	-	degradation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite
[Ref.: #24474]		acetoin ChEBI

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #24474]		acetoin ChEBI	+

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #24474]		arginine dihydrolase	-	3.5.3.6

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #24474]	Sample type/isolated from	soil and industrial amylase fermentations

[Ref.: #49266]	Sample type/isolated from	Soil

[Ref.: #2015]	Sample type/isolated from	soil
* marker position based on {}

Isolation sources categories

#Engineered	#Industrial	#Industrial production
#Environmental	#Terrestrial	#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence HQ231913 (>99% sequence identity) for Bacillus from Microbeatlas

Aquatic Samples	2854
Soil Samples	3768
Animal Samples	9674
Plant Samples	1905
Total Samples	18201

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #2015]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #2015]	Bacillus amyloliquefaciens gene for 16S rRNA, partial sequence	AB006920	274	ENA	692420 tax ID
[Ref.: #20218]	Zhouia sp. DSM7 16S ribosomal RNA gene, partial sequence	KC439236	910	ENA	1343290 tax ID	*
[Ref.: #20218]	Bacillus amyloliquefaciens strain DSM7 (F) 16S ribosomal RNA gene, partial sequence	AY055225	500	ENA	692420 tax ID	*
[Ref.: #20218]	Bacillus amyloliquefaciens strain MPA 1034 16S ribosomal RNA gene, partial sequence	HQ231913	1448	ENA	1390 tax ID	*
[Ref.: #20218]	B.amyloliquefaciens 16S ribosomal RNA	X60605	1427	ENA	692420 tax ID	*
[Ref.: #20218]	Bacillus amyloliquefaciens clone BamyloDH3R 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	AF478077	408	ENA	692420 tax ID	*
[Ref.: #20218]	Bacillus amyloliquefaciens clone DH-19 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	AF478078	466	ENA	692420 tax ID	*
[Ref.: #20218]	Bacillus amyloliquefaciens clone DH-11-1 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; tRNA-Ile and tRNA-Ala genes, complete sequence; and 23S ribosomal RNA gene, partial sequence	AF478079	584	ENA	692420 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Bacillus amyloliquefaciens DSM 7 = ATCC 23350 DSM 7	GCA_000196735	complete	GenBank	692420 tax ID	*

[Ref.: #24474]	GC-content	44.6 mol%
[Ref.: #2015]	GC-content	46.2 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #2015]	GC-content	44.5 mol%	Buoyant density centrifugation (BD)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Bacillus amyloliquefaciens DSM 7 = ATCC 23350 DSM 7 NCBI: GCA_000196735.1
[Ref.: #69480]	anaerobic	no	99.744	no
[Ref.: #69480]	gram-positive	yes	100	yes
[Ref.: #69480]	spore-forming	yes	100	no
[Ref.: #69480]	thermophile	no	99.53	no
[Ref.: #69480]	motile	yes	91.406	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Bacillus amyloliquefaciens DSM 7 = ATCC 23350 DSM 7 NCBI: GCA_000196735.1
[Ref.: #69481]	spore-forming	yes	100	no

Availability in culture collections External links

[Ref.: #2015]

Culture collection no.

DSM 7, ATCC 23350, CCUG 28519, NRRL B-14393, Fukumoto strain F

[Ref.: #20218]

Associated Passport(s) in StrainInfo

4420, 4417

Literature:

Only first 10 entries are displayed. Click here to see all.

Topic	Title	Authors	Journal	DOI	Year
Metabolism	Comparative Genome Analysis Reveals Phylogenetic Identity of Bacillus velezensis HNA3 and Genomic Insights into Its Plant Growth Promotion and Biocontrol Effects.	Zaid DS, Cai S, Hu C, Li Z, Li Y	Microbiol Spectr	10.1128/spectrum.02169-21	2022	*
Cultivation	Control Efficacy of Bacillus velezensis AFB2-2 against Potato Late Blight Caused by Phytophthora infestans in Organic Potato Cultivation.	Kim MJ, Shim CK, Park JH	Plant Pathol J	10.5423/PPJ.FT.09.2021.0138	2021	*
Cultivation	Triaging of Culture Conditions for Enhanced Secondary Metabolite Diversity from Different Bacteria.	Schwarz J, Hubmann G, Rosenthal K, Lutz S	Biomolecules	10.3390/biom11020193	2021	*
Genetics	Complete Genome of Bacillus velezensis CMT-6 and Comparative Genome Analysis Reveals Lipopeptide Diversity.	Deng Q, Wang R, Sun D, Sun L, Wang Y, Pu Y, Fang Z, Xu D, Liu Y, Ye R, Yin S, Xie S, Gooneratne R	Biochem Genet	10.1007/s10528-019-09927-z	2019	*
Genetics	Genome and transcriptome analysis of surfactin biosynthesis in Bacillus amyloliquefaciens MT45.	Zhi Y, Wu Q, Xu Y	Sci Rep	10.1038/srep40976	2017	*
Enzymology	Characterization of a Mannose-6-Phosphate Isomerase from Bacillus amyloliquefaciens and Its Application in Fructose-6-Phosphate Production.	Sigdel S, Singh R, Kim TS, Li J, Kim SY, Kim IW, Jung WS, Pan CH, Kang YC, Lee JK	PLoS One	10.1371/journal.pone.0131585	2015	*
Genetics	Complete genome sequence of Bacillus amyloliquefaciens TA208, a strain for industrial production of guanosine and ribavirin.	Zhang G, Deng A, Xu Q, Liang Y, Chen N, Wen T	J Bacteriol	10.1128/JB.00440-11	2011	*
Genetics	Genome sequence of B. amyloliquefaciens type strain DSM7(T) reveals differences to plant-associated B. amyloliquefaciens FZB42.	Ruckert C, Blom J, Chen X, Reva O, Borriss R	J Biotechnol	10.1016/j.jbiotec.2011.01.006	2011	*
Phylogeny	Relationship of Bacillus amyloliquefaciens clades associated with strains DSM 7T and FZB42T: a proposal for Bacillus amyloliquefaciens subsp. amyloliquefaciens subsp. nov. and Bacillus amyloliquefaciens subsp. plantarum subsp. nov. based on complete genome sequence comparisons.	Borriss R, Chen XH, Rueckert C, Blom J, Becker A, Baumgarth B, Fan B, Pukall R, Schumann P, Sproer C, Junge H, Vater J, Puhler A, Klenk HP	Int J Syst Evol Microbiol	10.1099/ijs.0.023267-0	2010	*
Metabolism	Phytate utilization by genetically engineered lysine-producing Corynebacterium glutamicum.	Tzvetkov MV, Liebl W	J Biotechnol	10.1016/j.jbiotec.2008.02.005	2008	*
Biotechnology	Cell growth and alpha-amylase production characteristics of Bacillus amyloliquefaciens.	Roychoudhury S, Parulekar SJ, Weigand WA	Biotechnol Bioeng	10.1002/bit.260330209	1989	*
Phylogeny	Bacteriophage SPO2-mediated plasmid transduction in transpositional mutagenesis within the genus Bacillus.	Kopec LK, Yasbin RE, Marrero R	J Bacteriol	10.1128/jb.164.3.1283-1287.1985	1985	*
	Bacillus amyloliquefaciens, Bacillus velezensis, and Bacillus siamensis Form an "Operational Group B. amyloliquefaciens" within the B. subtilis Species Complex.	Fan B, Blom J, Klenk HP, Borriss R	Front Microbiol	10.3389/fmicb.2017.00022	2017	*

References

#2015

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 7

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#24474

F. G. Priest, M. Goodfellow, L. A. Shute, R. C. W. Berkeley: Bacillus amyloliquefaciens sp. nov., nom. rev.. IJSEM 37: 69 - 71 1987 ( DOI 10.1099/00207713-37-1-69 )

#35323 ; Curators of the CIP;
#49266 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 28519

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

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Bacillus amyloliquefaciens DSM 7 = ATCC 23350 DSM 7

Bacillus amyloliquefaciens DSM 7 = ATCC 23350 DSM 7

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