Name: Chryseobacterium molle DW3
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 5699

Type strain:

Species: Chryseobacterium molle

Strain Designation: DW3

Culture col. no.: DSM 18016, CCUG 52547, CIP 110177

Strain history: CIP <- 2010, DSMZ <- A. Lipski, Osnabrück Univ., Osnabrück, Germany: strain DW3

NCBI tax ID(s): 216903 (species)

SI-ID 323956

CCUG 52547, DSM 18016, LMG 25630, CIP 110177

Section

Chryseobacterium molle DW3 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from biofilm of a beer-bottling plant.

Gram-negative
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacteroidota*
	Class	*Flavobacteriia*
	Order	*Flavobacteriales*
	Family	*Weeksellaceae*
	Genus	*Chryseobacterium*
	Species	**Chryseobacterium molle**
	Full Scientific Name (LPSN)	Chryseobacterium molle Herzog et al. 2008

Synonym

Epilithonimonas mollis

Information on morphological and physiological properties Morphology

[Ref.: #32348]	Gram stain	negative
[Ref.: #121004]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.947

[Ref.: #32348]	Cell length	1.3 µm

[Ref.: #32348]	Cell width	0.9 µm

[Ref.: #32348]	Cell shape	rod-shaped
[Ref.: #121004]	Cell shape	rod-shaped

[Ref.: #32348]	Motility	no
[Ref.: #121004]	Motility	no
[Ref.: #69480]	Motility	no Confidence in %94.663

[Ref.: #32348]

Pigment production

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7335]

Culture medium

TRYPTONE SOYA BROTH (TSB) (DSMZ Medium 545)

[Ref.: #7335]

Culture medium growth

[Ref.: #7335]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7335]

Culture medium composition

expand / minimize

[Ref.: #34714]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #34714]

Culture medium growth

[Ref.: #34714]

Culture medium composition

expand / minimize

[Ref.: #121004]

Culture medium

CIP Medium 72

[Ref.: #121004]

Culture medium growth

[Ref.: #121004]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #7335]	growth	28 °C
[Ref.: #32348]	growth	25-37 °C
[Ref.: #34714]	growth	30 °C
[Ref.: #59590]	growth	30 °C

[Ref.: #7335]	Temperature range	mesophilic
[Ref.: #32348]	Temperature range	mesophilic
[Ref.: #34714]	Temperature range	mesophilic
[Ref.: #59590]	Temperature range	mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #32348]	Oxygen tolerance	aerobe
[Ref.: #59590]	Oxygen tolerance	aerobe
[Ref.: #121004]	Oxygen tolerance	facultative anaerobe

[Ref.: #32348]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.994

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #32348]		NaCl		growth	<2	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #32348]	arabinose ChEBI	+	carbon source
[Ref.: #68371]	arbutin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #32348]	esculin ChEBI	+	hydrolysis
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	gluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #32348]	glucose ChEBI	+	carbon source
[Ref.: #68371]	glycerol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	lactose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #32348]	malate ChEBI	+	carbon source
[Ref.: #32348]	maltose ChEBI	+	carbon source
[Ref.: #32348]	mannose ChEBI	+	carbon source
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	melibiose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-mannoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #121004]	nitrate ChEBI	-	reduction
[Ref.: #121004]	nitrite ChEBI	+	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	salicin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	sucrose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	trehalose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #32348]		indole ChEBI	yes
[Ref.: #121004]		indole ChEBI	yes

	Physiological tests	Metabolite	Indole test
[Ref.: #32348]		indole ChEBI	+

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	+	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #32348]	catalase	+	1.11.1.6
[Ref.: #121004]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	+	3.4.11.3	* from API zym
[Ref.: #32348]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #32348]	gelatinase	+
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	+		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #121004]	oxidase	+
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #121004]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #121004]	11715	not determinedn.d.	-	-	-	-	-	+/-	-	-	-	-	+/-	+/-	+/-	-	-	-	-	-	-	-	-	-	+/-	-	+/-	-	+/-	+/-	-	-	-	-	-	-	-	+/-	+/-	-	+/-	-	-	-	-	-	-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #121004]	12415	-	+	+	+	+	+	+	+	+	+	+	+	-	-	-	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7335]	Sample type/isolated from	biofilm of a beer-bottling plant
[Ref.: #7335]	Country	Germany
[Ref.: #7335]	Country ISO 3 Code	DEU
[Ref.: #7335]	Continent	Europe

[Ref.: #59590]	Sample type/isolated from	Steel surface of a beer bottling plant
[Ref.: #59590]	Sampling date	2002-08-01
[Ref.: #59590]	Country	Germany
[Ref.: #59590]	Country ISO 3 Code	DEU
[Ref.: #59590]	Continent	Europe

[Ref.: #121004]	Sample type/isolated from	Biofilm of a beer-bottling plant
[Ref.: #121004]	Country	Germany
[Ref.: #121004]	Country ISO 3 Code	DEU
[Ref.: #121004]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Engineered	#Industrial	#Plant (Factory)
#Environmental	#Biofilm	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AJ534853 (>99% sequence identity) for Chryseobacterium molle from Microbeatlas

Aquatic Samples	164
Soil Samples	26
Animal Samples	254
Plant Samples	36
Total Samples	480

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #7335]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #121004]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #7335]	Chryseobacterium molle partial 16S rRNA gene, type strain DW3T	AJ534853	1421	ENA	216903 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Epilithonimonas mollis DSM 18016	GCA_900142325	contig	GenBank	216903 tax ID	*
[Ref.: #66792]	Chryseobacterium molle strain DSM 18016	216903.4	wgs	PATRIC	216903 tax ID	*
[Ref.: #66792]	Epilithonimonas mollis DSM 18016	2698536696	draft	JGI IMG/M	216903 tax ID	*

[Ref.: #32348]

GC-content

39.2 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Chryseobacterium molle strain DSM 18016 PATRIC: 216903.4
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Chryseobacterium molle strain DSM 18016 PATRIC: 216903.4
[Ref.: #69480]	motile	no	92.377	yes
[Ref.: #69480]	gram-positive	no	97.919	yes
[Ref.: #69480]	anaerobic	no	99.14	yes
[Ref.: #69480]	aerobic	yes	85.154	yes
[Ref.: #69480]	halophile	no	95.417	no
[Ref.: #69480]	spore-forming	no	95.722	yes
[Ref.: #69480]	glucose-util	yes	88.439	yes
[Ref.: #69480]	thermophile	no	99.635	yes
[Ref.: #69480]	flagellated	no	96.686	no
[Ref.: #69480]	glucose-ferment	no	89.383	no

Availability in culture collections External links

[Ref.: #7335]

Culture collection no.

DSM 18016, CCUG 52547, CIP 110177

[Ref.: #75181]

SI-ID 323956

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Chryseobacterium ureilyticum sp. nov., Chryseobacterium gambrini sp. nov., Chryseobacterium pallidum sp. nov. and Chryseobacterium molle sp. nov., isolated from beer-bottling plants.	Herzog P, Winkler I, Wolking D, Kampfer P, Lipski A	Int J Syst Evol Microbiol	10.1099/ijs.0.65362-0	2008	*

References

#7335

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 18016

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#32348

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28579 (see below)

#34714 ; Curators of the CIP;
#59590 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 52547

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68371

Automatically annotated from API 50CH acid .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#75181

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID323956.1 )

#121004 Collection of Institut Pasteur ; Curators of the CIP; CIP 110177

#28579

IJSEM 26 2008 ( DOI 10.1099/ijs.0.65362-0 , PubMed 18175677 )

* These data were automatically processed and therefore are not curated

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Chryseobacterium molle strain DSM 18016

Chryseobacterium molle strain DSM 18016

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