Flavobacterium reichenbachii (DSM 21791, CIP 109866, WB 3.2-61)

Name: Flavobacterium reichenbachii (DSM 21791, CIP 109866, WB 3.2-61)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 5577

Type strain

Strain Designation WB3.2-61

Culture col. no. DSM 21791 CIP 109866 WB 3.2-61 WB3.2-61

NCBI tax ID(s) 362418

Special Collections DSMZ CIP Literature strains

Links

Flavobacterium reichenbachii WB3.2-61 is an aerobe, psychrophilic, Gram-negative prokaryote that was isolated from water of a hardwater creek.

Gram-negative motile rod-shaped aerobe psychrophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 363446

DSM 21791,CIP 109866,LMG 25512

@ref 20215

Last LPSN update 2025-12-16 09:48:06

Domain Pseudomonadati

Phylum Bacteroidota

Class Flavobacteriia

Order Flavobacteriales

Family Flavobacteriaceae

Genus Flavobacterium

Species Flavobacterium reichenbachii

Full scientific name Flavobacterium reichenbachii Ali et al. 2009

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Flagellum arrangement	Confidence
29215	negative	6.75 µm	1 µm	rod-shaped	gliding
122150	negative			rod-shaped
125439	negative					99.9
125438						91
125438	negative					98.227

Pigmentation

29215

Productionyes

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
15954	R2A MEDIUM (DSMZ Medium 830)	Medium recipe at MediaDive	Name: R2A MEDIUM (DSMZ Medium 830) Composition: Agar 15.0 g/l Casamino acids 0.5 g/l Starch 0.5 g/l Glucose 0.5 g/l Proteose peptone 0.5 g/l Yeast extract 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
36126	MEDIUM 566- Reasoner's 2A agar for Flavobacterium micromati		Distilled water make up to (1000.000 ml);R2A agar (18.200 g)
122150	CIP Medium 566	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
15954	positive	growth	24	psychrophilic
29215	positive	growth	06-30
29215	positive	optimum	20-26
36126	positive	growth	25	mesophilic

Culture pH

@ref	Ability	Type	PH
29215	positive	optimum	6.2
29215	positive	growth	5.8-8.0

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
29215	aerobe
122150	obligate aerobe
125439	obligate aerobe	96.7

Spore formation

@ref	Spore formation	Confidence
125439		98.5

Halophily

@ref	Salt	Growth	Tested relation	Concentration
29215	NaCl	positive	growth	0-2 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
29215	22599 ChEBI	arabinose	+	carbon source
29215	22653 ChEBI	asparagine	+	carbon source
29215	35391 ChEBI	aspartate	+	carbon source
29215	17057 ChEBI	cellobiose	+	carbon source
29215	4853 ChEBI	esculin	+	hydrolysis
29215	28757 ChEBI	fructose	+	carbon source
29215	33984 ChEBI	fucose	+	carbon source
29215	24175 ChEBI	galacturonate	+	carbon source
29215	17234 ChEBI	glucose	+	carbon source
29215	17716 ChEBI	lactose	+	carbon source
29215	17306 ChEBI	maltose	+	carbon source
29215	37684 ChEBI	mannose	+	carbon source
29215	51850 ChEBI	methyl pyruvate	+	carbon source
29215	506227 ChEBI	N-acetylglucosamine	+	carbon source
122150	17632 ChEBI	nitrate	-	reduction
122150	16301 ChEBI	nitrite	-	reduction
29215	18257 ChEBI	ornithine	+	carbon source
29215	26271 ChEBI	proline	+	carbon source
29215	26986 ChEBI	threonine	+	carbon source
29215	53423 ChEBI	tween 40	+	carbon source
29215	16704 ChEBI	uridine	+	carbon source

Metabolite production

@ref	Chebi-ID	Metabolite	Production
122150	35581 ChEBI	indole

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68382	beta-galactosidase	+	3.2.1.23	from API zym
68382	beta-glucosidase	+	3.2.1.21	from API zym
68382	beta-glucuronidase	+	3.2.1.31	from API zym
29215	catalase	+	1.11.1.6
122150	catalase	+	1.11.1.6
68382	cystine arylamidase	+	3.4.11.3	from API zym
29215	cytochrome oxidase	+	1.9.3.1
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
29215	gelatinase	+
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
122150	oxidase	+
68382	trypsin	-	3.4.21.4	from API zym
122150	urease	-	3.5.1.5
68382	valine arylamidase	+		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
122150	-	+	+	+	-	+	+	+	-	-	+	+	-	+	+	+	+	+	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#River (Creek)

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
15954	water of a hardwater creek	Harz Mountains, Westerhöfer Bach, Westerhof, 40 km north of Göttingen	Germany	DEU	Europe
122150	Environment, Hard water, river	Westerhöfer Bach, Harz mountains	Germany	DEU	Europe

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
15954	1	Risk group (German classification) According to TRBA 466
122150	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM221743v1 assembly for Flavobacterium reichenbachii DSM 21791	scaffold	GCA_002217435	362418.11	8111395754	362418	68.9

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
15954	Flavobacterium reichenbachii partial 16S rRNA gene, type strain WB 3.2-61T	AM177616	1501		362418

GC content

@ref	GC-content (mol%)	Method
15954	34.3	high performance liquid chromatography (HPLC)
29215	34.3

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Flavobacterium reichenbachii DSM 21791
NCBI: GCA_002217435

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	98.50	no
125439	motility	BacteriaNetⓘ	no	68.00	no
125439	gram_stain	BacteriaNetⓘ	negative	99.90	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	96.70	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Flavobacterium reichenbachii DSM 21791
NCBI: GCA_002217435.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	98.23	no
125438	anaerobic	anaerobicⓘ	no	98.16	yes
125438	aerobic	aerobicⓘ	yes	87.02	yes
125438	spore-forming	spore-formingⓘ	no	85.12	no
125438	thermophilic	thermophileⓘ	no	98.42	yes
125438	flagellated	motile2+ⓘ	no	91.00	yes

Literature

Topic	Title	Authors	Journal	DOI	Year
	Phenotypic Characterization and Draft Genome Sequence Analyses of Two Novel Endospore-Forming Sporosarcina spp. Isolated from Canada Goose (Branta canadensis) Feces.	Keshri J, Smith KM, Svendsen MK, Keillor HR, Moss ML, Jordan HJ, Larkin AM, Garrish JK, Line JE, Ball PN, Oakley BB, Seal BS.	Microorganisms	10.3390/microorganisms12010070	2023
	Elucidating Sequence and Structural Determinants of Carbohydrate Esterases for Complete Deacetylation of Substituted Xylans.	Penttinen L, Kouhi V, Faure R, Skarina T, Stogios P, Master E, Jurak E.	Molecules	10.3390/molecules27092655	2022
Phylogeny	Flavobacterium fluviatile sp. nov., isolated from a freshwater creek.	Chen WM, Su CL, Young CC, Sheu SY	Int J Syst Evol Microbiol	10.1099/ijsem.0.002659	2018
Phylogeny	Flavobacterium palustre sp. nov., isolated from wetland soil.	Feng H, Zeng Y, Huang Y	Int J Syst Evol Microbiol	10.1099/ijs.0.000054	2015
Phylogeny	Flavobacterium banpakuense sp. nov., isolated from leaf-and-branch compost.	Kim JJ, Jin HM, Lee HJ, Jeon CO, Kanaya E, Koga Y, Takano K, Kanaya S	Int J Syst Evol Microbiol	10.1099/ijs.0.022467-0	2010
Phylogeny	Flavobacterium rivuli sp. nov., Flavobacterium subsaxonicum sp. nov., Flavobacterium swingsii sp. nov. and Flavobacterium reichenbachii sp. nov., isolated from a hard water rivulet.	Ali Z, Cousin S, Fruhling A, Brambilla E, Schumann P, Yang Y, Stackebrandt E	Int J Syst Evol Microbiol	10.1099/ijs.0.008771-0	2009

References

#15954	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 21791
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#29215	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #25636 (see below)
#36126	; Curators of the CIP;
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68382	Automatically annotated from API zym .
#122150	Collection of Institut Pasteur ; Curators of the CIP; CIP 109866
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Flavobacterium reichenbachii (DSM 21791, CIP 109866, WB 3.2-61)

Name and taxonomic classification

Morphology

Cell morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Metabolite production

Enzymes

API zym

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_002217435

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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