Name: Flavobacterium cheniae NJ-26
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 5572

Type strain:

Species: Flavobacterium cheniae

Strain Designation: NJ-26

Culture col. no.: DSM 22462, CGMCC 1.6844, NBRC 103934, CIP 109891, KACC 14365

Strain history: CIP <- 2008, NBRC <- J.H. Qu, China Agric. Univ., Beijing, China: strain NJ-26

NCBI tax ID(s): 295428 (species)

SI-ID 358875

CGMCC 1.6844, NBRC 103934, CIP 109891, KACC 14365, DSM 22462

Section

Flavobacterium cheniae NJ-26 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from sediment of the eutrophicated Guanting Reservoir.

Gram-negative
motile
rod-shaped
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacteroidota*
	Class	*Flavobacteriia*
	Order	*Flavobacteriales*
	Family	*Flavobacteriaceae*
	Genus	*Flavobacterium*
	Species	**Flavobacterium cheniae**
	Full Scientific Name (LPSN)	Flavobacterium cheniae Qu et al. 2008

Information on morphological and physiological properties Morphology

[Ref.: #32498]	Gram stain	negative
[Ref.: #118229]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.998

[Ref.: #32498]	Cell length	1.5-3 µm

[Ref.: #32498]	Cell width	0.3-0.5 µm

[Ref.: #32498]	Cell shape	rod-shaped
[Ref.: #118229]	Cell shape	rod-shaped

[Ref.: #32498]	Motility	yes
[Ref.: #118229]	Motility	no

[Ref.: #32498]	Flagellum arrangement	gliding

[Ref.: #32498]

Pigment production

yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #16351]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #16351]

Culture medium growth

[Ref.: #16351]

Culture medium link

Medium recipe at MediaDive

[Ref.: #16351]

Culture medium composition

expand / minimize

[Ref.: #39837]

Culture medium

MEDIUM 566- Reasoner's 2A agar for Flavobacterium micromati

[Ref.: #39837]

Culture medium growth

[Ref.: #39837]

Culture medium composition

expand / minimize

[Ref.: #118229]

Culture medium

CIP Medium 566

[Ref.: #118229]

Culture medium growth

[Ref.: #118229]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #16351]	growth	25 °C
[Ref.: #32498]	growth	18-32 °C
[Ref.: #32498]	optimum	28 °C
[Ref.: #39837]	growth	25 °C

[Ref.: #16351]	Temperature range	mesophilic
[Ref.: #32498]	Temperature range	mesophilic
[Ref.: #39837]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #32498]			optimum	7.5
[Ref.: #32498]			growth	6.5-8

Information on physiology and metabolism Physiology and metabolism

[Ref.: #32498]	Oxygen tolerance	aerobe
[Ref.: #118229]	Oxygen tolerance	obligate aerobe

[Ref.: #32498]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.999

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #32498]		NaCl		growth	0-0.8	%

[Ref.: #32498]

Observation

aggregates in chains

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #118229]		nitrate ChEBI	-	reduction
[Ref.: #118229]		nitrite ChEBI	-	reduction

	Metabolite production	Metabolite	Production
[Ref.: #32498]		hydrogen sulfide ChEBI	yes
[Ref.: #118229]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #32498]	alkaline phosphatase	+	3.1.3.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #32498]	catalase	+	1.11.1.6
[Ref.: #118229]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	+	3.4.11.3	* from API zym
[Ref.: #32498]	cytochrome oxidase	+	1.9.3.1
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #118229]	oxidase	+
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #118229]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #118229]	12197	-	+	+	+	-	+	+	+	+	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16351]	Sample type/isolated from	sediment of the eutrophicated Guanting Reservoir
[Ref.: #16351]	Geographic location (country and/or sea, region)	Beijing city
[Ref.: #16351]	Country	China
[Ref.: #16351]	Country ISO 3 Code	CHN
[Ref.: #16351]	Continent	Asia

[Ref.: #118229]	Sample type/isolated from	Environment, Sediment, Guanting reservoir
[Ref.: #118229]	Geographic location (country and/or sea, region)	Beijing
[Ref.: #118229]	Country	China
[Ref.: #118229]	Country ISO 3 Code	CHN
[Ref.: #118229]	Continent	Asia
[Ref.: #118229]	Isolation date	2006
* marker position based on {}

Isolation sources categories

#Engineered	#Built environment	#Water reservoir (Aquarium/pool)
#Environmental	#Aquatic	#Sediment

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #16351]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #118229]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #16351]	Flavobacterium cheniae 16S ribosomal RNA gene, partial sequence	EF407880	1410	ENA	295428 tax ID
[Ref.: #20218]	Flavobacterium cheniae gene for 16S rRNA, partial sequence, strain: NBRC 103934	AB682144	1439	ENA	295428 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Flavobacterium cheniae CGMCC 1.6844	GCA_007830415	contig	GenBank	295428 tax ID	*
[Ref.: #66792]	Flavobacterium cheniae DSM 22462	GCA_004363695	scaffold	GenBank	295428 tax ID	*
[Ref.: #66792]	Flavobacterium cheniae strain CGMCC 1.6844	295428.5	wgs	PATRIC	295428 tax ID	*
[Ref.: #66792]	Flavobacterium cheniae strain DSM 22462	295428.4	wgs	PATRIC	295428 tax ID	*
[Ref.: #66792]	Flavobacterium cheniae CGMCC 1.6844	2596583558	draft	JGI IMG/M	295428 tax ID	*
[Ref.: #66792]	Flavobacterium cheniae DSM 22462	2757320365	draft	JGI IMG/M	295428 tax ID	*

[Ref.: #16351]

GC-content

40.6 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Flavobacterium cheniae DSM 22462 NCBI: GCA_004363695.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Flavobacterium cheniae DSM 22462 NCBI: GCA_004363695.1
[Ref.: #69480]	motile	no	88.443	no
[Ref.: #69480]	flagellated	no	95.612	yes
[Ref.: #69480]	gram-positive	no	99.05	yes
[Ref.: #69480]	anaerobic	no	98.68	yes
[Ref.: #69480]	aerobic	yes	85.612	yes
[Ref.: #69480]	halophile	no	92.762	yes
[Ref.: #69480]	spore-forming	no	96.273	yes
[Ref.: #69480]	glucose-util	yes	83.286	no
[Ref.: #69480]	thermophile	no	98.495	yes
[Ref.: #69480]	glucose-ferment	no	91.495	no

Availability in culture collections External links

[Ref.: #16351]

Culture collection no.

DSM 22462, CGMCC 1.6844, NBRC 103934, CIP 109891, KACC 14365

[Ref.: #75055]

SI-ID 358875

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Flavobacterium proteolyticum sp. nov., isolated from aquaculture water.	Du J, Liu Y, Pei T, Li A, Deng MR, Zhu H	Arch Microbiol	10.1007/s00203-021-02744-2	2022	*
Phylogeny	Flavobacterium jumunjinense sp. nov., isolated from a lagoon, and emended descriptions of Flavobacterium cheniae, Flavobacterium dongtanense and Flavobacterium gelidilacus.	Joung Y, Kim H, Joh K	Int J Syst Evol Microbiol	10.1099/ijs.0.045286-0	2013	*
Phylogeny	Flavobacterium aquaticum sp. nov., isolated from a water sample of a rice field.	Subhash Y, Sasikala C, Ramana CV	Int J Syst Evol Microbiol	10.1099/ijs.0.050047-0	2013	*
Phylogeny	Flavobacterium dongtanense sp. nov., isolated from the rhizosphere of a wetland reed.	Xiao YP, Hui W, Lee JS, Lee KC, Quan ZX	Int J Syst Evol Microbiol	10.1099/ijs.0.022301-0	2010	*
Phylogeny	Flavobacterium cheniae sp. nov., isolated from sediment of a eutrophic reservoir.	Qu JH, Li HF, Yang JS, Yuan HL	Int J Syst Evol Microbiol	10.1099/ijs.0.65586-0	2008	*

References

#16351

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22462

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#32498

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #28718 (see below)

#39837 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#75055

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID358875.1 )

#118229 Collection of Institut Pasteur ; Curators of the CIP; CIP 109891

#28718

IJSEM 2186 2008 ( DOI 10.1099/ijs.0.65586-0 , PubMed 18768627 )

* These data were automatically processed and therefore are not curated

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Flavobacterium cheniae DSM 22462

Flavobacterium cheniae DSM 22462

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