Name: Thermosphaera aggregans M11TL
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 4205

Type strain:

Species: Thermosphaera aggregans

Strain Designation: M11TL

Culture col. no.: DSM 11486

Strain history: <- K. O. Stetter, Univ. Regensburg, Germany; M11TL <- R. Huber and K. O. Stetter {1994}

NCBI tax ID(s): 633148 (strain), 54254 (species)

SI-ID 49120

DSM 11486

Section

Thermosphaera aggregans M11TL is an anaerobe, heterotroph, hyperthermophilic archaeon that was isolated from hot spring.

coccus-shaped
motile
anaerobe
heterotroph
hyperthermophilic
16S sequence
Archaea
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Archaea"
	Phylum	*Thermoproteota*
	Class	*Thermoprotei*
	Order	*Desulfurococcales*
	Family	*Desulfurococcaceae*
	Genus	*Thermosphaera*
	Species	**Thermosphaera aggregans**
	Full Scientific Name (LPSN)	Thermosphaera aggregans Huber et al. 1998

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Gram stain	negative Confidence in %99.964

[Ref.: #23228]	Cell length	0.2-0.8 µm

[Ref.: #23228]	Cell shape	coccus-shaped

[Ref.: #23228]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %92.621

[Ref.: #23228]

Colony color

bluish-green fluorescence at 420 nm

Information on culture and growth conditions Culture and growth conditions

[Ref.: #4384]

Culture medium

THERMOSPHAERA MEDIUM (DSMZ Medium 817)

[Ref.: #4384]

Culture medium growth

[Ref.: #4384]

Culture medium link

Medium recipe at MediaDive

[Ref.: #4384]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #4384]	growth	85 °C
[Ref.: #23228]	growth	67-90 °C
[Ref.: #23228]	optimum	85 °C

[Ref.: #4384]	Temperature range	hyperthermophilic
[Ref.: #23228]	Temperature range	hyperthermophilic
[Ref.: #69480]	Temperature range	thermophilic Confidence in %100

	pH	Kind of pH		pH
[Ref.: #23228]			optimum	6.5
[Ref.: #23228]			growth	5.0-7.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #4384]	Oxygen tolerance	anaerobe
[Ref.: #23228]	Oxygen tolerance	anaerobe
[Ref.: #69480]	Oxygen tolerance	anaerobe Confidence in %100

[Ref.: #23228]

Nutrition type

heterotroph

[Ref.: #23228]	Ability of spore formation	no
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.974

	Metabolite utilization	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23228]		yeast extract	+	growth

	Pathway	Enzyme coverage	Annotated reactions
	Pathway annotation	Computationally determined by
[Ref.: #66794]	dolichol and dolichyl phosphate biosynthesis	100	2 of 2
[Ref.: #66794]	anapleurotic synthesis of oxalacetate	100	1 of 1
[Ref.: #66794]	ribulose monophosphate pathway	100	2 of 2
[Ref.: #66794]	teichoic acid biosynthesis	100	1 of 1
[Ref.: #66794]	UDP-GlcNAc biosynthesis	100	3 of 3
[Ref.: #66794]	starch degradation	90	9 of 10
[Ref.: #66794]	molybdenum cofactor biosynthesis	88.89	8 of 9
[Ref.: #66794]	pantothenate biosynthesis	83.33	5 of 6
[Ref.: #66794]	cellulose degradation	80	4 of 5
[Ref.: #66794]	palmitate biosynthesis	77.27	17 of 22
[Ref.: #66794]	glycolysis	76.47	13 of 17
[Ref.: #66794]	glycogen biosynthesis	75	3 of 4
[Ref.: #66794]	coenzyme A metabolism	75	3 of 4
[Ref.: #66794]	acetate fermentation	75	3 of 4
[Ref.: #66794]	vitamin B1 metabolism	69.23	9 of 13
[Ref.: #66794]	aspartate and asparagine metabolism	66.67	6 of 9
[Ref.: #66794]	enterobactin biosynthesis	66.67	2 of 3
[Ref.: #66794]	acetoin degradation	66.67	2 of 3
[Ref.: #66794]	L-lactaldehyde degradation	66.67	2 of 3
[Ref.: #66794]	photosynthesis	64.29	9 of 14
[Ref.: #66794]	glutamate and glutamine metabolism	64.29	18 of 28
[Ref.: #66794]	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	62.5	5 of 8
[Ref.: #66794]	purine metabolism	61.7	58 of 94
[Ref.: #66794]	glycogen metabolism	60	3 of 5
[Ref.: #66794]	lipoate biosynthesis	60	3 of 5
[Ref.: #66794]	pyrimidine metabolism	60	27 of 45
[Ref.: #66794]	propanol degradation	57.14	4 of 7
[Ref.: #66794]	serine metabolism	55.56	5 of 9
[Ref.: #66794]	d-mannose degradation	55.56	5 of 9
[Ref.: #66794]	NAD metabolism	55.56	10 of 18
[Ref.: #66794]	pentose phosphate pathway	54.55	6 of 11
[Ref.: #66794]	phenylalanine metabolism	53.85	7 of 13
[Ref.: #66794]	polyamine pathway	52.17	12 of 23
[Ref.: #66794]	dTDPLrhamnose biosynthesis	50	4 of 8
[Ref.: #66794]	degradation of sugar alcohols	50	8 of 16
[Ref.: #66794]	suberin monomers biosynthesis	50	1 of 2
[Ref.: #66794]	ketogluconate metabolism	50	4 of 8
[Ref.: #66794]	CDP-diacylglycerol biosynthesis	50	1 of 2
[Ref.: #66794]	sulfopterin metabolism	50	2 of 4
[Ref.: #66794]	cis-vaccenate biosynthesis	50	1 of 2
[Ref.: #66794]	ethanol fermentation	50	1 of 2
[Ref.: #66794]	biotin biosynthesis	50	2 of 4
[Ref.: #66794]	glycine metabolism	50	5 of 10
[Ref.: #66794]	adipate degradation	50	1 of 2
[Ref.: #66794]	Entner Doudoroff pathway	50	5 of 10
[Ref.: #66794]	selenocysteine biosynthesis	50	3 of 6
[Ref.: #66794]	oxidative phosphorylation	46.15	42 of 91
[Ref.: #66794]	urea cycle	46.15	6 of 13
[Ref.: #66794]	lysine metabolism	45.24	19 of 42
[Ref.: #66794]	lipid metabolism	45.16	14 of 31
[Ref.: #66794]	alanine metabolism	44.83	13 of 29
[Ref.: #66794]	valine metabolism	44.44	4 of 9
[Ref.: #66794]	flavin biosynthesis	40	6 of 15
[Ref.: #66794]	factor 420 biosynthesis	40	2 of 5
[Ref.: #66794]	phenol degradation	40	8 of 20
[Ref.: #66794]	myo-inositol biosynthesis	40	4 of 10
[Ref.: #66794]	propionate fermentation	40	4 of 10
[Ref.: #66794]	cysteine metabolism	38.89	7 of 18
[Ref.: #66794]	degradation of hexoses	38.89	7 of 18
[Ref.: #66794]	sulfate reduction	38.46	5 of 13
[Ref.: #66794]	methionine metabolism	38.46	10 of 26
[Ref.: #66794]	gluconeogenesis	37.5	3 of 8
[Ref.: #66794]	C4 and CAM-carbon fixation	37.5	3 of 8
[Ref.: #66794]	tryptophan metabolism	36.84	14 of 38
[Ref.: #66794]	dolichyl-diphosphooligosaccharide biosynthesis	36.36	4 of 11
[Ref.: #66794]	vitamin B6 metabolism	36.36	4 of 11
[Ref.: #66794]	glutathione metabolism	35.71	5 of 14
[Ref.: #66794]	tyrosine metabolism	35.71	5 of 14
[Ref.: #66794]	sulfoquinovose degradation	33.33	1 of 3
[Ref.: #66794]	lipid A biosynthesis	33.33	3 of 9
[Ref.: #66794]	IAA biosynthesis	33.33	1 of 3
[Ref.: #66794]	CO2 fixation in Crenarchaeota	33.33	3 of 9
[Ref.: #66794]	acetyl CoA biosynthesis	33.33	1 of 3
[Ref.: #66794]	glycolate and glyoxylate degradation	33.33	2 of 6
[Ref.: #66794]	formaldehyde oxidation	33.33	1 of 3
[Ref.: #66794]	octane oxidation	33.33	1 of 3
[Ref.: #66794]	arginine metabolism	33.33	8 of 24
[Ref.: #66794]	leucine metabolism	30.77	4 of 13
[Ref.: #66794]	threonine metabolism	30	3 of 10
[Ref.: #66794]	mevalonate metabolism	28.57	2 of 7
[Ref.: #66794]	degradation of pentoses	28.57	8 of 28
[Ref.: #66794]	cardiolipin biosynthesis	28.57	2 of 7
[Ref.: #66794]	ubiquinone biosynthesis	28.57	2 of 7
[Ref.: #66794]	citric acid cycle	28.57	4 of 14
[Ref.: #66794]	ascorbate metabolism	27.27	6 of 22
[Ref.: #66794]	proline metabolism	27.27	3 of 11
[Ref.: #66794]	d-xylose degradation	27.27	3 of 11
[Ref.: #66794]	isoprenoid biosynthesis	26.92	7 of 26
[Ref.: #66794]	peptidoglycan biosynthesis	26.67	4 of 15
[Ref.: #66794]	non-pathway related	26.32	10 of 38
[Ref.: #66794]	ppGpp biosynthesis	25	1 of 4
[Ref.: #66794]	butanoate fermentation	25	1 of 4
[Ref.: #66794]	catecholamine biosynthesis	25	1 of 4
[Ref.: #66794]	isoleucine metabolism	25	2 of 8
[Ref.: #66794]	toluene degradation	25	1 of 4
[Ref.: #66794]	cyclohexanol degradation	25	1 of 4
[Ref.: #66794]	degradation of aromatic, nitrogen containing compounds	25	3 of 12
[Ref.: #66794]	tetrahydrofolate metabolism	21.43	3 of 14
		Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #4384]	Sample type/isolated from	hot spring
[Ref.: #4384]	Geographic location (country and/or sea, region)	Yellowstone Natl. Park, Obsidian Pool
[Ref.: #4384]	Country	USA
[Ref.: #4384]	Country ISO 3 Code	USA
[Ref.: #4384]	Continent	North America
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Thermal spring
#Condition	#Thermophilic (>45°C)	-

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #4384]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #4384]	T.aggregans 16S rRNA gene	X99556	1432	ENA	633148 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Thermosphaera aggregans DSM 11486	GCA_000092185	complete	GenBank	633148 tax ID	*
[Ref.: #66792]	Thermosphaera aggregans M11TL, DSM 11486	646564583	complete	JGI IMG/M	633148 tax ID	*
[Ref.: #66792]	Thermosphaera aggregans DSM 11486	633148.11	complete	PATRIC	633148 tax ID	*

[Ref.: #4384]	GC-content	46 mol%	thermal denaturation, midpoint method (Tm)
[Ref.: #23228]	GC-content	46.0 mol%	Thermal denaturation, fluorometry

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Thermosphaera aggregans DSM 11486 DSM 11486 NCBI: GCA_000092185.1
[Ref.: #69481]	spore-forming	no	86	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Thermosphaera aggregans DSM 11486 DSM 11486 NCBI: GCA_000092185.1
[Ref.: #69480]	motile	no	61.038	yes
[Ref.: #69480]	flagellated	no	91.585	no
[Ref.: #69480]	gram-positive	no	94.866	no
[Ref.: #69480]	anaerobic	yes	99.408	yes
[Ref.: #69480]	aerobic	no	96.832	yes
[Ref.: #69480]	halophile	no	81.821	no
[Ref.: #69480]	spore-forming	no	97.706	no
[Ref.: #69480]	thermophile	yes	100	yes
[Ref.: #69480]	glucose-util	yes	79.719	no
[Ref.: #69480]	glucose-ferment	no	57.34	no

Availability in culture collections External links

[Ref.: #4384]

Culture collection no.

DSM 11486

[Ref.: #73705]

SI-ID 49120

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Complete genome sequence of Thermosphaera aggregans type strain (M11TL).	Spring S, Rachel R, Lapidus A, Davenport K, Tice H, Copeland A, Cheng JF, Lucas S, Chen F, Nolan M, Bruce D, Goodwin L, Pitluck S, Ivanova N, Mavromatis K, Ovchinnikova G, Pati A, Chen A, Palaniappan K, Land M, Hauser L, Chang YJ, Jeffries CC, Brettin T, Detter JC, Tapia R, Han C, Heimerl T, Weikl F, Brambilla E, Goker M, Bristow J, Eisen JA, Markowitz V, Hugenholtz P, Kyrpides NC, Klenk HP	Stand Genomic Sci	10.4056/sigs.821804	2010	*
Phylogeny	Sulfur-inhibited Thermosphaera aggregans sp. nov., a new genus of hyperthermophilic archaea isolated after its prediction from environmentally derived 16S rRNA sequences.	Huber R, Dyba D, Huber H, Burggraf S, Rachel R	Int J Syst Bacteriol	10.1099/00207713-48-1-31	1998	*

References

#4384

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 11486

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#23228

Robert Huber, Diana Dyba, Harald Huber, Siegfried Burggraf, Reinhard Rachel: Sulfur-inhibited Thermosphaera aggregans sp. nov., a new genus of hyperthermophilic archaea isolated after its prediction from environmentally derived 16S rRNA sequences. IJSEM 48: 31 - 38 1998 ( DOI 10.1099/00207713-48-1-31 , PubMed 9542073 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66794

Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#73705

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID49120.1 )

* These data were automatically processed and therefore are not curated

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Thermosphaera aggregans DSM 11486 DSM 11486

Thermosphaera aggregans DSM 11486 DSM 11486

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