Archive BacDiveID:10.13145/bacdive4205.20230509.8.1

Strain identifier

BacDive ID: 4205

Type strain:

Species: Thermosphaera aggregans

Strain Designation: M11TL

Strain history: <- K. O. Stetter, Univ. Regensburg, Germany; M11TL <- R. Huber and K. O. Stetter {1994}

NCBI tax ID(s): 633148 (strain), 54254 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 4384

BacDive-ID: 4205

DSM-Number: 11486

keywords: genome sequence, 16S sequence, Archaea, anaerobe, heterotroph, hyperthermophilic, motile, coccus-shaped

description: Thermosphaera aggregans M11TL is an anaerobe, heterotroph, hyperthermophilic archaeon that was isolated from hot spring.

NCBI tax id

NCBI tax id	Matching level
633148	strain
54254	species

strain history

@ref: 4384
history: <- K. O. Stetter, Univ. Regensburg, Germany; M11TL <- R. Huber and K. O. Stetter {1994}

doi: 10.13145/bacdive4205.20230509.8.1

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/archaea
keyword: phylum/thermoproteota
domain: Archaea
phylum: Thermoproteota
class: Thermoprotei
order: Desulfurococcales
family: Desulfurococcaceae
genus: Thermosphaera
species: Thermosphaera aggregans
full scientific name: Thermosphaera aggregans Huber et al. 1998

@ref: 4384

domain: Archaea

phylum: Crenarchaeota

class: Thermoprotei

order: Desulfurococcales

family: Desulfurococcaceae

genus: Thermosphaera

species: Thermosphaera aggregans

full scientific name: Thermosphaera aggregans Huber et al. 1998

strain designation: M11TL

type strain: yes

Morphology

cell morphology

@ref	cell length	cell shape	motility	confidence	gram stain
23228	0.2-0.8 µm	coccus-shaped	yes
69480			yes	92.621
69480				99.964	negative

colony morphology

@ref: 23228
colony color: bluish-green fluorescence at 420 nm

Culture and growth conditions

culture medium

@ref: 4384
name: THERMOSPHAERA MEDIUM (DSMZ Medium 817)
growth: yes
link: https://mediadive.dsmz.de/medium/817
composition: Name: THERMOSPHAERA MEDIUM (DSMZ Medium 817) Composition: MgCl2 x 6 H2O 2.16749 g/l Yeast extract 0.985222 g/l Peptone 0.985222 g/l NaCl 0.886699 g/l Na2CO3 0.492611 g/l Na2S x 9 H2O 0.492611 g/l KCl 0.0167488 g/l NH4Cl 0.0118227 g/l CaCl2 x 2 H2O 0.00689655 g/l K2HPO4 x 3 H2O 0.00689655 g/l Sodium resazurin 0.000492611 g/l Pyridoxine hydrochloride 9.85222e-05 g/l Nicotinic acid 4.92611e-05 g/l FeCl3 4.92611e-05 g/l (DL)-alpha-Lipoic acid 4.92611e-05 g/l p-Aminobenzoic acid 4.92611e-05 g/l Calcium D-(+)-pantothenate 4.92611e-05 g/l Thiamine HCl 4.92611e-05 g/l Riboflavin 4.92611e-05 g/l Folic acid 1.97044e-05 g/l Biotin 1.97044e-05 g/l Vitamin B12 9.85222e-07 g/l Distilled water

culture temp

@ref	growth	type	temperature	range	confidence
4384	positive	growth	85	hyperthermophilic
23228	positive	growth	67-90	hyperthermophilic
23228	positive	optimum	85	hyperthermophilic
69480				thermophilic	100

culture pH

@ref	ability	type	pH
23228	positive	growth	5.0-7.0
23228	positive	optimum	6.5

Physiology and metabolism

oxygen tolerance

@ref	oxygen tolerance	confidence
4384	anaerobe
23228	anaerobe
69480	anaerobe	100

nutrition type

@ref: 23228
type: heterotroph

spore formation

@ref	spore formation	confidence
23228	no
69480	no	99.974

metabolite utilization

@ref: 23228
metabolite: yeast extract
utilization activity: +
kind of utilization tested: growth

Isolation, sampling and environmental information

isolation

@ref: 4384
sample type: hot spring
geographic location: Yellowstone Natl. Park, Obsidian Pool
country: USA
origin.country: USA
continent: North America

isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Thermal spring
#Condition	#Thermophilic (>45°C)

Safety information

risk assessment

@ref: 4384
biosafety level: 1
biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@ref: 4384
description: T.aggregans 16S rRNA gene
accession: X99556
length: 1432
database: ena
NCBI tax ID: 633148

Genome sequences

@ref	description	accession	assembly level	database	NCBI tax ID
66792	Thermosphaera aggregans DSM 11486	GCA_000092185	complete	ncbi	633148
66792	Thermosphaera aggregans M11TL, DSM 11486	646564583	complete	img	633148
66792	Thermosphaera aggregans DSM 11486	633148.11	complete	patric	633148

GC content

@ref	GC-content	method
4384	46	thermal denaturation, midpoint method (Tm)
23228	46.0	Thermal denaturation, fluorometry

Genome-based predictions

predictions

trait	prediction	confidence	training_data
spore-forming	no	86	no
motile	no	61.038	yes
flagellated	no	91.585	no
gram-positive	no	94.866	no
anaerobic	yes	99.408	yes
aerobic	no	96.832	yes
halophile	no	81.821	no
spore-forming	no	97.706	no
thermophile	yes	100	yes
glucose-util	yes	79.719	no
glucose-ferment	no	57.34	no

External links

@ref: 4384

culture collection no.: DSM 11486

straininfo link

@ref: 73705
straininfo: 49120

literature

topic	Pubmed-ID	title	authors	journal	DOI	year	mesh	topic2
Phylogeny	9542073	Sulfur-inhibited Thermosphaera aggregans sp. nov., a new genus of hyperthermophilic archaea isolated after its prediction from environmentally derived 16S rRNA sequences.	Huber R, Dyba D, Huber H, Burggraf S, Rachel R	Int J Syst Bacteriol	10.1099/00207713-48-1-31	1998	Bacteriological Techniques, Cell Membrane/chemistry/ultrastructure, DNA, Archaeal/analysis, Desulfurococcaceae/classification/genetics/metabolism, Freeze Fracturing, Lipids/analysis, Molecular Sequence Data, Nucleic Acid Hybridization, Phylogeny, RNA, Archaeal/analysis, RNA, Ribosomal, 16S/analysis, Sulfur/*metabolism, Temperature	Metabolism
Genetics	21304709	Complete genome sequence of Thermosphaera aggregans type strain (M11TL).	Spring S, Rachel R, Lapidus A, Davenport K, Tice H, Copeland A, Cheng JF, Lucas S, Chen F, Nolan M, Bruce D, Goodwin L, Pitluck S, Ivanova N, Mavromatis K, Ovchinnikova G, Pati A, Chen A, Palaniappan K, Land M, Hauser L, Chang YJ, Jeffries CC, Brettin T, Detter JC, Tapia R, Han C, Heimerl T, Weikl F, Brambilla E, Goker M, Bristow J, Eisen JA, Markowitz V, Hugenholtz P, Kyrpides NC, Klenk HP	Stand Genomic Sci	10.4056/sigs.821804	2010

Reference

@id	authors	catalogue	doi/url	title	journal	pubmed
4384	Curators of the DSMZ	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 11486)	https://www.dsmz.de/collection/catalogue/details/culture/DSM-11486
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.		10.1099/ijsem.0.004332	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
23228	Robert Huber, Diana Dyba, Harald Huber, Siegfried Burggraf, Reinhard Rachel		10.1099/00207713-48-1-31	Sulfur-inhibited Thermosphaera aggregans sp. nov., a new genus of hyperthermophilic archaea isolated after its prediction from environmentally derived 16S rRNA sequences	IJSEM 48: 31-38 1998	9542073
66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg		10.1093/nar/gkaa1025	BRENDA, the ELIXIR core data resource in 2021: new developments and updates	Nucleic Acids Res. 49: D498-D508 2020	33211880
69480	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
73705	Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.		10.60712/SI-ID49120.1	StrainInfo: A central database for resolving microbial strain identifiers