Name: Hugenholtzia roseola CR-155
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 3792

Type strain:

Species: Hugenholtzia roseola

Strain Designation: CR-155

Culture col. no.: DSM 9546, ATCC 23088, IFO 16707, NBRC 16707, CIP 106406, LMG 13856, NCIMB 1433

Strain history: CIP <- 2000, J.F. Bernardet, INRA, Jouy en Josas, France <- ATCC <- R.A. Lewin: strain CR-155

NCBI tax ID(s): 1121903 (strain), 1002 (species)

SI-ID 2842

LMG 3993, ATCC 23088, DSM 9546, LMG 13856, LMG 13168, NCIMB 1433, LMG 13507, CIP 106406, IFO 16707, IFO16486, IFO 16031, NBRC 16486, NBRC 16707

Special Collections

DSMZ
CIP

Section

Hugenholtzia roseola CR-155 is a Gram-negative bacterium that was isolated from hot spring.

Gram-negative
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacteroidota*
	Class	*Cytophagia*
	Order	*Cytophagales*
	Family	*Bernardetiaceae*
	Genus	*Hugenholtzia*
	Species	**Hugenholtzia roseola**
	Full Scientific Name (LPSN)	Hugenholtzia roseola (Lewin 1969) Hahnke et al. 2017

Synonym

Flexibacter roseolus

Information on morphological and physiological properties Morphology

[Ref.: #122257]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %96.978

[Ref.: #122257]	Cell shape	rod-shaped

[Ref.: #122257]	Motility	no

[Ref.: #122257]	Pigment production	no
[Ref.: #122257]	Pigment name	Flexirubin

Information on culture and growth conditions Culture and growth conditions

[Ref.: #3635]

Culture medium

FLEXIBACTER CANADENSIS MEDIUM (DSMZ Medium 357)

[Ref.: #3635]

Culture medium growth

[Ref.: #3635]

Culture medium link

Medium recipe at MediaDive

[Ref.: #3635]

Culture medium composition

expand / minimize

[Ref.: #42266]

Culture medium

MEDIUM 33 - For Flexibacter flexilis and Flexibacter elegans

[Ref.: #42266]

Culture medium growth

[Ref.: #42266]

Culture medium composition

expand / minimize

[Ref.: #122257]

Culture medium

CIP Medium 33

[Ref.: #122257]

Culture medium growth

[Ref.: #122257]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #3635]		growth	30 °C
[Ref.: #42266]		growth	25 °C
[Ref.: #122257]		growth	15-37 °C
[Ref.: #122257]	no	growth	5 °C
[Ref.: #122257]	no	growth	41 °C
[Ref.: #122257]	no	growth	45 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69480]	Ability of spore formation	no Confidence in %91.5
[Ref.: #69481]	Ability of spore formation	no Confidence in %100

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #122257]	esculin ChEBI	-	hydrolysis
[Ref.: #122257]	hippurate ChEBI	-	hydrolysis
[Ref.: #122257]	nitrate ChEBI	-	reduction
[Ref.: #122257]	nitrite ChEBI	-	reduction

	Metabolite production	Metabolite	Production
[Ref.: #122257]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #122257]		acetoin ChEBI	-
[Ref.: #122257]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #122257]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	+	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #122257]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #122257]	catalase	-	1.11.1.6
[Ref.: #68382]	cystine arylamidase	+	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #122257]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #122257]	gelatinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	+		* from API zym
[Ref.: #122257]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #122257]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #122257]	oxidase	+
[Ref.: #122257]	phenylalanine ammonia-lyase	-	4.3.1.24
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #122257]	tryptophan deaminase	-
[Ref.: #122257]	tween esterase	+
[Ref.: #122257]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #122257]	9474	-	+	+	+	+	+	+	+	+	+	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #3635]	Sample type/isolated from	hot spring
[Ref.: #3635]	Country	Costa Rica
[Ref.: #3635]	Country ISO 3 Code	CRI
[Ref.: #3635]	Continent	Middle and South America

[Ref.: #122257]	Sample type/isolated from	Environment, Hot spring
[Ref.: #122257]	Geographic location (country and/or sea, region)	Agua Caliente
[Ref.: #122257]	Country	Costa Rica
[Ref.: #122257]	Country ISO 3 Code	CRI
[Ref.: #122257]	Continent	North America
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Thermal spring
#Condition	#Thermophilic (>45°C)	-

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #3635]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #122257]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #3635]	Hugenholtzia roseola strain NBRC 16707 16S ribosomal RNA, partial sequence	NR_113862	1441	GenBank ENA	1002 tax ID
[Ref.: #20218]	Flexibacter roseolus 16S ribosomal RNA	M58787	1466	GenBank ENA	1002 tax ID	*
[Ref.: #20218]	Flexibacter roseolus gene for 16S rRNA, strain:IFO 16486	AB078062	1461	GenBank ENA	1002 tax ID	*
[Ref.: #20218]	Flexibacter roseolus gene for 16S rRNA, strain:IFO 16707	AB078063	1471	GenBank ENA	1002 tax ID	*
[Ref.: #20218]	Flexibacter roseolus gene for 16S rRNA, partial sequence, strain: NBRC 16486	AB681070	1431	GenBank ENA	1002 tax ID	*
[Ref.: #20218]	Flexibacter roseolus gene for 16S rRNA, partial sequence, strain: NBRC 16707	AB681110	1441	GenBank ENA	1002 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Hugenholtzia roseola DSM 9546	GCA_000422585	scaffold	GenBank ENA	1121903 tax ID	*
[Ref.: #66792]	Hugenholtzia roseola DSM 9546	1121903.5	wgs	PATRIC	1121903 tax ID	*
[Ref.: #66792]	Hugenholtzia roseola DSM 9546	2523533540	draft	JGI IMG/M	1121903 tax ID	*

[Ref.: #3635]

GC-content

42.2 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Hugenholtzia roseola DSM 9546 DSM 9546 NCBI: GCA_000422585.1
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Hugenholtzia roseola DSM 9546 DSM 9546 NCBI: GCA_000422585.1
[Ref.: #69480]	gram-positive	gram-positiveⓘ	no	96.978	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	96.879	no
[Ref.: #69480]	aerobic	aerobicⓘ	yes	78.974	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	91.5	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	84.285	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	87.923	no

Availability in culture collections External links

[Ref.: #3635]

Culture collection no.

DSM 9546, ATCC 23088, IFO 16707, NBRC 16707, CIP 106406, LMG 13856, NCIMB 1433

[Ref.: #73304]

SI-ID 2842

References

#3635

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 9546

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#42266 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#73304

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID2842.1 )

#122257 Collection of Institut Pasteur ; Curators of the CIP; CIP 106406
* These data were automatically processed and therefore are not curated

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Hugenholtzia roseola DSM 9546 DSM 9546

Hugenholtzia roseola DSM 9546 DSM 9546

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