Strain identifier

BacDive ID: 3792

Type strain: Yes

Species: Hugenholtzia roseola

Strain Designation: CR-155

Strain history: CIP <- 2000, J.F. Bernardet, INRA, Jouy en Josas, France <- ATCC <- R.A. Lewin: strain CR-155

NCBI tax ID(s): 1121903 (strain), 1002 (species)

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General

@ref: 3635

BacDive-ID: 3792

DSM-Number: 9546

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, Gram-negative

description: Hugenholtzia roseola CR-155 is a mesophilic, Gram-negative bacterium that was isolated from hot spring.

NCBI tax id

NCBI tax idMatching level
1121903strain
1002species

strain history

@refhistory
3635<- ATCC <- R. A. Lewin, CR-155
122257CIP <- 2000, J.F. Bernardet, INRA, Jouy en Josas, France <- ATCC <- R.A. Lewin: strain CR-155

doi: 10.13145/bacdive3792.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacteroidota
  • domain: Bacteria
  • phylum: Bacteroidota
  • class: Cytophagia
  • order: Cytophagales
  • family: Bernardetiaceae
  • genus: Hugenholtzia
  • species: Hugenholtzia roseola
  • full scientific name: Hugenholtzia roseola (Lewin 1969) Hahnke et al. 2017

  • synonyms

    • @ref: 20215
    • synonym: Flexibacter roseolus

@ref: 3635

domain: Bacteria

phylum: Bacteroidetes

class: Cytophagia

order: Cytophagales

family: Bernardetiaceae

genus: Hugenholtzia

species: Hugenholtzia roseola

full scientific name: Hugenholtzia roseola (Lewin 1969) Hahnke et al. 2017

strain designation: CR-155

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480negative99.897
122257negativerod-shapedno

pigmentation

  • @ref: 122257
  • production: no
  • name: Flexirubin

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3635FLEXIBACTER CANADENSIS MEDIUM (DSMZ Medium 357)yeshttps://mediadive.dsmz.de/medium/357Name: FLEXIBACTER CANADENSIS MEDIUM (DSMZ Medium 357) Composition: Agar 10.0 g/l Tris 1.0 g/l Glucose 1.0 g/l Casamino acids 1.0 g/l Sodium glycerophosphate 0.1 g/l CaCl2 x 2 H2O 0.1 g/l KNO3 0.1 g/l MgSO4 x 7 H2O 0.1 g/l HCl 0.0025 g/l FeCl2 x 4 H2O 0.0015 g/l Thiamine-HCl x 2 H2O 0.001 g/l CoCl2 x 6 H2O 0.00019 g/l MnCl2 x 4 H2O 0.0001 g/l ZnCl2 7e-05 g/l Na2MoO4 x 2 H2O 3.6e-05 g/l NiCl2 x 6 H2O 2.4e-05 g/l H3BO3 6e-06 g/l CuCl2 x 2 H2O 2e-06 g/l Vitamin B12 1e-06 g/l Distilled water
42266MEDIUM 33 - For Flexibacter flexilis and Flexibacter elegansyesDistilled water make up to (1000.000 ml);Magnesium sulphate heptahydrate (0.100 g);Calcium chloride dihydrate (0.100 g);Glucose (1.000 g);Potassium nitrate (0.100 g);Casamino acids (1.000 g);Sodium glycerophosphate hexahydrate (0.100 g);Trizma hydrochlori
122257CIP Medium 33yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=33

culture temp

@refgrowthtypetemperaturerange
3635positivegrowth30mesophilic
42266positivegrowth25mesophilic
122257positivegrowth15-37
122257nogrowth5psychrophilic
122257nogrowth41thermophilic
122257nogrowth45thermophilic

Physiology and metabolism

spore formation

@refspore formationconfidence
69481no100
69480no99.786

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1222574853esculin-hydrolysis
122257606565hippurate-hydrolysis
12225717632nitrate-reduction
12225716301nitrite-reduction

metabolite production

  • @ref: 122257
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12225715688acetoin-
12225717234glucose-

enzymes

@refvalueactivityec
68382alpha-fucosidase-3.2.1.51
68382alpha-mannosidase-3.2.1.24
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382beta-glucosidase-3.2.1.21
68382alpha-glucosidase-3.2.1.20
68382beta-glucuronidase-3.2.1.31
68382beta-galactosidase-3.2.1.23
68382alpha-galactosidase-3.2.1.22
68382naphthol-AS-BI-phosphohydrolase+
68382acid phosphatase+3.1.3.2
68382alpha-chymotrypsin+3.4.21.1
68382trypsin+3.4.21.4
68382cystine arylamidase+3.4.11.3
68382valine arylamidase+
68382leucine arylamidase+3.4.11.1
68382lipase (C 14)+
68382esterase lipase (C 8)+
68382esterase (C 4)+
68382alkaline phosphatase+3.1.3.1
122257oxidase+
122257beta-galactosidase-3.2.1.23
122257alcohol dehydrogenase-1.1.1.1
122257gelatinase-
122257catalase-1.11.1.6
122257tween esterase+
122257gamma-glutamyltransferase-2.3.2.2
122257lysine decarboxylase-4.1.1.18
122257ornithine decarboxylase-4.1.1.17
122257phenylalanine ammonia-lyase-4.3.1.24
122257tryptophan deaminase-
122257urease-3.5.1.5

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122257-+++++++++++--------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentgeographic location
3635hot springCosta RicaCRIMiddle and South America
122257Environment, Hot springCosta RicaCRINorth AmericaAgua Caliente

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Thermal spring
#Condition#Thermophilic (>45°C)

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
36351Risk group (German classification)
1222571Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Flexibacter roseolus 16S ribosomal RNAM587871466ena1002
20218Flexibacter roseolus gene for 16S rRNA, strain:IFO 16486AB0780621461ena1002
20218Flexibacter roseolus gene for 16S rRNA, strain:IFO 16707AB0780631471ena1002
20218Flexibacter roseolus gene for 16S rRNA, partial sequence, strain: NBRC 16486AB6810701431ena1002
20218Flexibacter roseolus gene for 16S rRNA, partial sequence, strain: NBRC 16707AB6811101441ena1002
3635Hugenholtzia roseola strain NBRC 16707 16S ribosomal RNA, partial sequenceNR_1138621441nuccore1002

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Hugenholtzia roseola DSM 9546GCA_000422585scaffoldncbi1121903
66792Hugenholtzia roseola DSM 95461121903.5wgspatric1121903
66792Hugenholtzia roseola DSM 95462523533540draftimg1121903

GC content

  • @ref: 3635
  • GC-content: 42.2

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno90.052no
flagellatedno95.202no
gram-positiveno98.826no
anaerobicno96.545no
aerobicyes73.25no
halophileno77.517no
spore-formingno92.344no
thermophileno96.55yes
glucose-utilyes73.038no
glucose-fermentno90no

External links

@ref: 3635

culture collection no.: DSM 9546, ATCC 23088, IFO 16707, NBRC 16707, CIP 106406, LMG 13856, NCIMB 1433

straininfo link

  • @ref: 73304
  • straininfo: 2842

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3635Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 9546)https://www.dsmz.de/collection/catalogue/details/culture/DSM-9546
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
42266Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18532
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
73304Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID2842.1StrainInfo: A central database for resolving microbial strain identifiers
122257Curators of the CIPCollection of Institut Pasteur (CIP 106406)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106406