Name: Corynebacterium pilosum 46 Hara
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 3108

Type strain:

Species: Corynebacterium pilosum

Strain Designation: 46 Hara

Culture col. no.: CCUG 27193, JCM 3714, NCTC 11862, CIP 103422, CCM 6140, DSM 20521, ATCC 29592, CECT 4049, CGMCC 1.1931, IFO 15285, KCTC 3434, LMG 19068, NBRC 15285

Strain history: CIP <- 1990, ATCC <- R. Yanagawa, Japan: strain 46 Hara <- K. Hirato, Japan

NCBI tax ID(s): 1121366 (strain), 35756 (species)

SI-ID 13101

LMG 19068, ATCC 29592, CCM 6140, CCUG 27193, CIP 103422, DSM 20521, IMET 11382, JCM 3714, NCTC 11862, IFO 15285, CECT 4049, KCTC 3434, IFO 15283, NBRC 15285, CGMCC 1.1931

Special Collections

Other strains from Corynebacterium pilosum

Section

Corynebacterium pilosum 46 Hara is a Gram-positive bacterium that was isolated from urine of cow.

Gram-positive
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Mycobacteriales*
	Family	*Corynebacteriaceae*
	Genus	*Corynebacterium*
	Species	**Corynebacterium pilosum**
	Full Scientific Name (LPSN)	Corynebacterium pilosum Yanagawa and Honda 1978 (Approved Lists 1980)

Information on morphological and physiological properties Morphology

[Ref.: #69480]	Motility	no Confidence in %93.5

[Ref.: #18444]	Colony color	Colorless
[Ref.: #18444]	Incubation period	10-14 days
[Ref.: #18444]	Cultivation medium used	ISP 6

[Ref.: #18444]	Multicellular complex forming ability	no
[Ref.: #18444]	Medium Name (multicellularity)	ISP 6

Information on culture and growth conditions Culture and growth conditions

[Ref.: #18444]

Culture medium

ISP 6

[Ref.: #18444]

Culture medium growth

[Ref.: #18444]

Culture medium composition

expand / minimize

[Ref.: #8890]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #8890]

Culture medium growth

[Ref.: #8890]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8890]

Culture medium composition

expand / minimize

[Ref.: #40635]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #40635]

Culture medium growth

[Ref.: #40635]

Culture medium composition

expand / minimize

[Ref.: #121832]

Culture medium

CIP Medium 72

[Ref.: #121832]

Culture medium growth

[Ref.: #121832]

Culture medium link

Medium recipe provided by DSMZ

		Temperature
[Ref.: #8890]	growth	37 °C
[Ref.: #18444]	optimum	37 °C
[Ref.: #40635]	growth	37 °C
[Ref.: #48680]	growth	37 °C
[Ref.: #67770]	growth	37 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #69481]

Ability of spore formation

no Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #18444]		NaCl		maximum	7.5	%

[Ref.: #8890]	Murein short key	A31
[Ref.: #8890]	Murein types	A1gamma m-Dpm-direct

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68368]	amygdalin ChEBI	-	fermentation	* from API 20E
[Ref.: #18444]	arabinose ChEBI	-
[Ref.: #68368]	arginine ChEBI	-	hydrolysis	* from API 20E
[Ref.: #18444]	cellulose ChEBI	-
[Ref.: #68368]	citrate ChEBI	-	assimilation	* from API 20E
[Ref.: #68368]	D-glucose ChEBI	+	fermentation	* from API 20E, API Coryne
[Ref.: #68368]	D-mannitol ChEBI	-	fermentation	* from API 20E, API Coryne
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68379]	esculin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #18444]	fructose ChEBI	+/-
[Ref.: #68368]	gelatin ChEBI	-	hydrolysis	* from API 20E, API Coryne
[Ref.: #18444]	glucose ChEBI	+
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #68368]	L-arabinose ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	L-rhamnose ChEBI	-	fermentation	* from API 20E
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68368]	lysine ChEBI	-	degradation	* from API 20E
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #18444]	mannitol ChEBI	-
[Ref.: #68368]	melibiose ChEBI	-	fermentation	* from API 20E
[Ref.: #18444]	myo-inositol ChEBI	-
[Ref.: #68368]	myo-inositol ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	ornithine ChEBI	-	degradation	* from API 20E
[Ref.: #18444]	raffinose ChEBI	-
[Ref.: #18444]	rhamnose ChEBI	-
[Ref.: #68368]	sorbitol ChEBI	-	fermentation	* from API 20E
[Ref.: #18444]	sucrose ChEBI	-
[Ref.: #68368]	sucrose ChEBI	-	fermentation	* from API 20E
[Ref.: #68368]	tryptophan ChEBI	-	energy source	* from API 20E
[Ref.: #68368]	urea ChEBI	+	hydrolysis	* from API 20E, API Coryne
[Ref.: #18444]	xylose ChEBI	-
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #68368]	acetoin ChEBI	yes	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68368]	indole ChEBI	no	* from API 20E

	Physiological tests	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #68368]		acetoin ChEBI	+		* from API 20E
[Ref.: #68368]		indole ChEBI		-	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #68382]	alkaline phosphatase	-	3.1.3.1	* from API zym, API Coryne
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym, API Coryne
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #68368]	arginine dihydrolase	-	3.5.3.6	* from API 20E
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym, API Coryne, API 20E
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym, API Coryne
[Ref.: #68382]	beta-glucuronidase	+	3.2.1.31	* from API zym
[Ref.: #68379]	catalase	+	1.11.1.6	* from API Coryne
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	-		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #68379]	gelatinase	-		* from API Coryne, API 20E
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #68368]	lysine decarboxylase	-	4.1.1.18	* from API 20E
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #68368]	ornithine decarboxylase	-	4.1.1.17	* from API 20E
[Ref.: #68379]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #68379]	urease	+	3.5.1.5	* from API Coryne, API 20E
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #18444]	34	-	-	-	-	-	-	+	-	-	+	-	+	-	-	-	-	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #18444]	121	-	-	-	-	+	-	-	-	-	+	-	not determinedn.d.	+	-	-	-	-	-	+	-	not determinedn.d.
[Ref.: #8890]	4309	+	+	-	-	+/-	-	-	+/-	-	+	-	-	+	+	-	-	-	-	-	-	+

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #18444]	156	not determinedn.d.	-	-	+	-	+	-	-	+	-	-	-	-	-	+	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #8890]	Sample type/isolated from	urine of cow

[Ref.: #48680]	Sample type/isolated from	Cow
[Ref.: #48680]	Country	Japan
[Ref.: #48680]	Country ISO 3 Code	JPN
[Ref.: #48680]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Urine of cow

[Ref.: #121832]	Sample type/isolated from	Animal, Cow
[Ref.: #121832]	Country	Japan
[Ref.: #121832]	Country ISO 3 Code	JPN
[Ref.: #121832]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Host	#Mammals	#Bovinae (Cow, Cattle)
#Host Body Product	#Fluids	#Urine

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence X81908 (>99% sequence identity) for Corynebacterium pilosum subclade from Microbeatlas

Aquatic Samples	95
Soil Samples	198
Animal Samples	2984
Plant Samples	43
Total Samples	3320

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #18444]	Biosafety level	2	German classification
[Ref.: #8890]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #121832]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Corynebacterium pilosum 16S rRNA gene, strain NCTC 11862	X84246	1440	GenBank ENA	35756 tax ID	*
[Ref.: #20218]	Corynebacterium pilosum gene for 16S rRNA	D37915	1324	GenBank ENA	35756 tax ID	*
[Ref.: #20218]	C.pilosum 16S rRNA gene (ATCC 29592T)	X81908	1381	GenBank ENA	35756 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Corynebacterium pilosum NCTC11862	GCA_900447205	contig	GenBank ENA	35756 tax ID
[Ref.: #67770]	Corynebacterium pilosum DSM 20521	GCA_000373805	scaffold	GenBank ENA	1121366 tax ID
[Ref.: #67770]	Corynebacterium pilosum CIP103422	GCA_001044155	contig	GenBank ENA	35756 tax ID
[Ref.: #66792]	Corynebacterium pilosum strain CIP103422	35756.11	wgs	PATRIC	35756 tax ID	*
[Ref.: #66792]	Corynebacterium pilosum strain NCTC11862	35756.13	wgs	PATRIC	35756 tax ID	*
[Ref.: #66792]	Corynebacterium pilosum DSM 20521	2515154153	draft	JGI IMG/M	1121366 tax ID	*

[Ref.: #8890]	GC-content	59.8 mol%
[Ref.: #67770]	GC-content	62 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67770]	GC-content	60.9 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Corynebacterium pilosum DSM 20521 DSM 20521 NCBI: GCA_000373805.1
[Ref.: #69481]	spore-forming	spore-formingⓘ	no	100	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Corynebacterium pilosum DSM 20521 PATRIC: 1121366.3
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	89.965	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	92.967	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	no	78.912	no
[Ref.: #69480]	aerobic	aerobicⓘ	yes	54.691	no
[Ref.: #69480]	thermophilic	thermophileⓘ	no	96	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	93.5	no

Availability in culture collections External links

[Ref.: #8890]

Culture collection no.

CCUG 27193, JCM 3714, NCTC 11862, CIP 103422, CCM 6140, DSM 20521, ATCC 29592, CECT 4049, CGMCC 1.1931, IFO 15285, KCTC 3434, LMG 19068, NBRC 15285

[Ref.: #72626]

SI-ID 13101

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Draft Genome Sequences of Two Pathogenic Corynebacterial Species Isolated from Cows.	Guimaraes LC, Lopes T, Ramos RT, Carneiro AR, Cavalcante AL, Barreto D, de Sa PC, Veras AA, Rocha FS, Bagano P, Pereira FL, Dorella FA, Leal CA, Carvalho AF, Bizet C, Guiso N, Badell E, Figueiredo HC, Azevedo V, Silva A	J Genomics	10.7150/jgen.14456	2016	*
Phylogeny	Corynebacterium lubricantis sp. nov., isolated from a coolant lubricant.	Kampfer P, Lodders N, Warfolomeow I, Falsen E, Busse HJ	Int J Syst Evol Microbiol	10.1099/ijs.0.006379-0	2009	*

References

#8890

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20521

#18444

Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig .

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#40635 ; Curators of the CIP;
#48680 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 27193

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68368

Automatically annotated from API 20E .

#68379

Automatically annotated from API Coryne .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#72626

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID13101.1 )

#121832 Collection of Institut Pasteur ; Curators of the CIP; CIP 103422
* These data were automatically processed and therefore are not curated

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Corynebacterium pilosum DSM 20521 DSM 20521

Corynebacterium pilosum DSM 20521

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